Monocyte Chemotactic Protein 4 (MCP4) Active Protein | MCP4 active protein

Active Monocyte Chemotactic Protein 4 (MCP4)

Cat. Num. AAA146618

• Gene Names: CCL13; NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13
• Applications: Activity Assay, Cell Culture
• Purity: > 95%
• Synonyms: Monocyte Chemotactic Protein 4 (MCP4); N/A; Active Monocyte Chemotactic Protein 4 (MCP4); CCL13; SCYA13; NCC1; SCYL1; CKb10; Chemokine C-C-Motif Ligand 13; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 13; CK-beta-10; Monocyte chemoattractant 4; MCP4 active protein

• Host: Yeast
• Purity/Purification: > 95%
• Form/Format: Freeze-dried powder
• Sequence Length: 98

• Applicable Applications for MCP4 active protein: Activity Assay, Cell Culture
• Fragment: Phe17~Thr98
• Tag: N-terminal His Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Organism Species: Human
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Application Data

Application Data

Application Data

SDS-PAGE

Activity

(CCL13 (C-C motif chemokine 13) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, which belongs to the CC chemokine subfamily. It has been reported that CCL13 can induce chemotactic migration of THP-1 cells. Therefore, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of CCL13 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension, 106 cells/mL in RPMI 1640 with 0.5% FBS) and CCL13 (50ng/mL and 100ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37oC with 5% CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).Result: CCL13 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen to count the migrated cells at high magnification (×400) and the statistical data was shown in Figure 2.)

Product Categories/Family for MCP4 active protein

Active Protein

NCBI and Uniprot Product Information

• NCBI GI #: 4885587
• NCBI GeneID: 6357
• NCBI Accession #: NP_005399.1
• NCBI GenBank Nucleotide #: NM_005408.2
• UniProt Accession #: Q99616; O95689; Q6ICQ6
• Molecular Weight: 10.7kDa; 11kDa
• NCBI Official Full Name: C-C motif chemokine 13
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 13
• NCBI Official Symbol: CCL13
• NCBI Official Synonym Symbols: NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13
• NCBI Protein Information: C-C motif chemokine 13
• UniProt Protein Name: C-C motif chemokine 13
• UniProt Gene Name: CCL13
• UniProt Synonym Gene Names: MCP4; NCC1; SCYA13; MCP-4