Standard Curve (Sample)
Human thiamin pyrophosphokinase 1 ELISA Kit | TPK1 elisa kit
Human Thiamin pyrophosphokinase 1, TPK1 ELISA Kit
Gene Names
TPK1; PP20; HTPK1; THMD5
Reactivity
Human
Synonyms
thiamin pyrophosphokinase 1; N/A; Human Thiamin pyrophosphokinase 1, TPK1 ELISA Kit; Human Thiamin pyrophosphokinase 1 (TPK1) ELISA kit; HTPK1; PP20; placental protein 20; thiamine diphosphokinase; thiamine kinase; TPK1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human TPK1. No significant cross-reactivity or interference between human TPK1 and analogues was observed.
Sequence Length
243
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
31.2 5 pg/ml -2000 pg/ml.
Sensitivity
The minimum detectable dose of human TPK1 is typically less than 7.8 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TPK1 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPK1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,265 Da
NCBI Official Full Name
thiamin pyrophosphokinase 1 isoform a
NCBI Official Synonym Full Names
thiamin pyrophosphokinase 1
NCBI Official Symbol
TPK1
NCBI Official Synonym Symbols
PP20; HTPK1; THMD5
NCBI Protein Information
thiamin pyrophosphokinase 1; thiamine kinase; placental protein 20; thiamine diphosphokinase
UniProt Protein Name
Thiamin pyrophosphokinase 1
UniProt Gene Name
TPK1
UniProt Synonym Gene Names
hTPK1; PP20
UniProt Entry Name
TPK1_HUMAN
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Product Notes
The Human TPK1 tpk1 (Catalog #AAA112258) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA112258 ELISA Kit recognizes Human TPK1. It is sometimes possible for the material contained within the vial of "thiamin pyrophosphokinase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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