Sequence Length
41
Preparation and Storage
Store at 4 degree C
Related Product Information for TCGF elisa kit
Mouse IL-2 (aka TCGF) is a 149 amino acid 15 kDa disulfide-linked monomeric glycosylate Type I cytokine. IL-2 induces proliferation of a variety of lymphocytes including CD4+ and CD8+ T cells, natural killer cells, and B cells. Serum IL-2 levels are elevated in several cancers and autoimmune conditions and lymphocyte secretion is decreased in HIV patients. IL-2 binds to the IL-2R receptor which is composed of three subunits of varying affinity. The sensitive quantitative measurement of total mouse IL-2 antigen in plasma, serum, cell culture media, or other biological fluids samples is easily performed with this 96 well strip format ELISA kit. The assay measures total mouse IL-2 in the 0.01-10 ng/ml range. Samples giving mouse IL-2 levels above 10 ng/ml should be diluted in culture media or blocking buffer before use. Mouse IL-2 will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated anti-mouse IL-2 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of IL-2 in the samples. A standard calibration curve is prepared using dilutions of purified IL-2 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Product Categories/Family for TCGF elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
4,762 Da
NCBI Official Full Name
IL-2, partial
NCBI Official Synonym Full Names
interleukin 2
NCBI Official Symbol
IL2
NCBI Official Synonym Symbols
IL-2; TCGF; lymphokine
NCBI Protein Information
interleukin-2
UniProt Protein Name
IL-2 protein
UniProt Gene Name
IL-2
UniProt Entry Name
O95974_HUMAN
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Product Notes
The TCGF il-2 (Catalog #AAA37852) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IL-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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