Search results for "Cell Culture"

BDNF, ELISA Kit (Cat# AAA13497)

Full Name

BDNF Human ELISA Kit

Reactivity

Human

Applications

ELISA

Pricing

M-CSF, ELISA Kit (Cat# AAA13520)

Full Name

M-CSF Human ELISA Kit

Reactivity

Human

Applications

ELISA

Pricing

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Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)

MARCO, Monoclonal Antibody (Cat# AAA12099)

Full Name

RAT ANTI MOUSE MARCO

Gene Names

Marco; Ly112; Scara2; AI323439

Applications

Immunohistochemistry, Immunofluorescence, Western Blot

Pricing

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE)

CD14, Monoclonal Antibody (Cat# AAA11841)

Full Name

MOUSE ANTI HUMAN CD14:APC

Applications

Flow Cytometry

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27689)

Full Name

VPS35 Antibody, Clone 8A3: PerCP

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

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DECTIN-1, Monoclonal Antibody (Cat# AAA12136)

Full Name

RAT ANTI MOUSE DECTIN-1:RPE

Gene Names

Clec7a; BGR; beta-GR; Clecsf12

Applications

Flow Cytometry

Pricing

Stromal cell Derived factor 1Alpha, ELISA Kit (Cat# AAA16170)

Full Name

Human Stromal cell Derived factor 1Alpha ELISA Kit

Gene Names

CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12

Reactivity

Human

Pricing

Clara Cell Protein 16 (CC16), ELISA Kit (Cat# AAA23117)

Full Name

Mouse Clara Cell Protein 16 (CC16) Mini Samples ELISA Kit

Gene Names

Scgb1a1; UG; UGB; Utg; CC10; CC16; CCSP; PCB-BP

Reactivity

Mouse

Applications

ELISA

Pricing

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CD107b, Monoclonal Antibody (Cat# AAA12260)

Full Name

Mouse Anti Dog CD107b

Applications

Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Pricing

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Application Data (Staining of human peripheral blood granulocytes with CD18: Alexa Fluor 647)

CD18, Monoclonal Antibody (Cat# AAA11881)

Full Name

RAT ANTI HUMAN CD18:FITC

Gene Names

ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1

Applications

Flow Cytometry

Pricing

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Application Data (Staining of human peripheral blood granulocytes with CD18: Alexa Fluor 647)

CD18, Monoclonal Antibody (Cat# AAA11987)

Full Name

RAT ANTI HUMAN CD18

Gene Names

ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Pricing

NP (H5N1) (A/Indonesia/5/2005), Recombinant Protein (Cat# AAA13755)

Full Name

NP (H5N1) (A/Indonesia/5/2005)

Applications

Western Blot

Purity

> 95% (SDS-PAGE)

Pricing

S100 Calcium Binding Protein A12, Recombinant Protein (Cat# AAA10893)

Full Name

Recombinant Human S100 Calcium Binding Protein A12

Gene Names

S100A12; p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27682)

Full Name

VPS35 Antibody, Clone 8A3

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

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DECTIN-1, Monoclonal Antibody (Cat# AAA12131)

Full Name

RAT ANTI MOUSE DECTIN-1:FITC

Gene Names

Clec7a; BGR; beta-GR; Clecsf12

Applications

Flow Cytometry

Pricing

H5 (H5N1) (A/Anhui/1/2005), Recombinant Protein (Cat# AAA13760)

Full Name

H5 (H5N1) (A/Anhui/1/2005) (aa 18-530)

Applications

Western Blot

Purity

> 95% pure by 10% SDS-PAGE gel

Pricing

Nuclei, Monoclonal Antibody (Cat# AAA14228)

Full Name

Anti-Nuclei

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry

Pricing

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Application Data (Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:FITC)

CD61, Monoclonal Antibody (Cat# AAA11873)

Full Name

HAMSTER ANTI MOUSE CD61:FITC

Gene Names

Itgb3; CD61; GP3A; INGRB3

Applications

Flow Cytometry

Pricing

MDA5/NF-kBT, Cell Line (Cat# AAA14874)

Full Name

MDA5/NF-kB Leeporter Luciferase Reporter-HEK293T Cell Line

Applications

Functional Assay

Pricing

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Macrophage HAM-56, Monoclonal Antibody (Cat# AAA13562)

Full Name

Macrophage HAM-56

Reactivity

Human, Monkey

Applications

Immunohistochemistry

Pricing

gp120 (HIV-1/Clade B), Recombinant Protein (Cat# AAA13743)

Full Name

gp120 (HIV-1/Clade B) (amino acid 24-518)

Applications

Western Blot

Purity

> 95% purity (SDS-PAGE)

Pricing

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Application Data (Fig.2: CGE analysis of purified EQ-8D11-C1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2ug of purified EQ-8D11-C1 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by CGE (Fig.2).)

Ovine CD34, Monoclonal Antibody (Cat# AAA14097)

Full Name

anti-ovine CD34 monoclonal antibody

Applications

Flow Cytometry, Competitive ELISA

Purity

Protein G purified

Pricing

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DECTIN-1, Monoclonal Antibody (Cat# AAA12133)

Full Name

RAT ANTI MOUSE DECTIN-1:FITC

Gene Names

Clec7a; BGR; beta-GR; Clecsf12

Applications

Flow Cytometry

Pricing

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DECTIN-1, Monoclonal Antibody (Cat# AAA12135)

Full Name

RAT ANTI MOUSE DECTIN-1:RPE

Gene Names

Clec7a; BGR; beta-GR; Clecsf12

Applications

Flow Cytometry

Pricing

Carbonic Anhydrase IX, Monoclonal Antibody (Cat# AAA14680)

Full Name

Carbonic Anhydrase IX (CA9) (PE)

Gene Names

CA9; MN; CAIX

Applications

Flow Cytometry

Purity

Affinity Purified
Purified by Protein A affinity chromatography from hybridoma culture supernant.

Pricing

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CD8 ALPHA, Monoclonal Antibody (Cat# AAA11982)

Full Name

MOUSE ANTI RAT CD8 ALPHA

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry

Pricing

HA1 (H1N1)(A/PR/8/34), Recombinant Protein (Cat# AAA13748)

Full Name

HA1 (H1N1) (aa 18-345)(A/PR/8/34)

Applications

Western Blot

Purity

> 95% by SDS PAGE

Pricing

C Reactive Protein (CRP), ELISA Kit (Cat# AAA23109)

Full Name

Human C Reactive Protein (CRP) High Sensitive ELISA Kit

Gene Names

CRP; PTX1

Reactivity

Human

Applications

ELISA

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27694)

Full Name

VPS35 Antibody, Clone 10A8: ATTO 594

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE)

CD14, Monoclonal Antibody (Cat# AAA11997)

Full Name

MOUSE ANTI HUMAN CD14

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Pricing

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CD4, Monoclonal Antibody (Cat# AAA12071)

Full Name

MOUSE ANTI HUMAN CD4:FITC

Gene Names

CD4; CD4mut

Reactivity

Human

Applications

Flow Cytometry

Pricing

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DECTIN-1, Monoclonal Antibody (Cat# AAA12126)

Full Name

RAT ANTI MOUSE DECTIN-1

Gene Names

Clec7a; BGR; beta-GR; Clecsf12

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Pricing

thyroxine, T4, ELISA Kit (Cat# AAA29737)

Full Name

Rat thyroxine, T4 ELISA Kit

Reactivity

Rat

Pricing

CD29, Monoclonal Antibody (Cat# AAA14855)

Full Name

anti-human CD29-Purified Preservative Free

Gene Names

ITGB1; CD29

Reactivity

Human, Pig, Mouse

Applications

Flow Cytometry, Western Blot

Purity

Purified Preservative Free

Pricing

CD4, Monoclonal Antibody (Cat# AAA14857)

Full Name

anti-human CD4-Purified Preservative Free

Gene Names

CD4; CD4mut

Applications

Flow Cytometry

Pricing

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CD4, Monoclonal Antibody (Cat# AAA11995)

Full Name

MOUSE ANTI RAT CD4 (DOMAIN 1)

Gene Names

Cd4; p55; W3/25

Applications

Immunohistochemistry, Flow Cytometry, Immunohistochemistry

Pricing

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Staining Procedure (1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides.2. Air dry for 2 hours at 58° C.3. Deparaffinize, dehydrate and rehydrate tissues.4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.5. Any of three heating methods may be used:a. TintoRetriever Pressure Cooker or EquivalentPlace tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. TintoRetriever PT Module or Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.c. Conventional Steamer MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. 8. Wash slides with ImmunoDNA washer or DI water. 9. Continue IHC staining protocol.)

Podoplanin/D2-40, Monoclonal Antibody (Cat# AAA13558)

Full Name

Podoplanin/D2-40

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry

Pricing

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CD8 ALPHA, Monoclonal Antibody (Cat# AAA11983)

Full Name

MOUSE ANTI RAT CD8 ALPHA

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot

Pricing

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DECTIN-1, Monoclonal Antibody (Cat# AAA12134)

Full Name

RAT ANTI MOUSE DECTIN-1

Gene Names

Clec7a; BGR; beta-GR; Clecsf12

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Pricing

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Application Data (Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)

IFN GAMMA, Monoclonal Antibody (Cat# AAA12093)

Full Name

MOUSE ANTI BOVINE INTERFERON GAMMA

Reactivity

Dog, Dolphin, Ferret, Fin Whale, Goat, Horse, Human, Mink, Rabbit, Pig, Sheep.
Based on sequence similarity, is expected to react with: Mustelid
N.B. Antibody reactivity and working conditions may vary between species.

Applications

Flow Cytometry

Pricing

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Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD195:FITC)

CD195, Monoclonal Antibody (Cat# AAA11952)

Full Name

RAT ANTI HUMAN CD195

Gene Names

CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5

Reactivity

Human

Applications

Flow Cytometry

Pricing

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CD102, Monoclonal Antibody (Cat# AAA11904)

Full Name

RAT ANTI MOUSE CD102:Low Endotoxin

Gene Names

Icam2; CD102; Ly-60; Icam-2

Applications

Flow Cytometry, Functional Assay, Immunoprecipitation

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27705)

Full Name

VPS35 Antibody, Clone 11H10: FITC

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Purity

Protein G Purified

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27685)

Full Name

VPS35 Antibody, Clone 8A3: ATTO 594

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

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CD4, Monoclonal Antibody (Cat# AAA11994)

Full Name

MOUSE ANTI RAT CD4 (DOMAIN 1)

Gene Names

Cd4; p55; W3/25

Applications

Immunohistochemistry, Flow Cytometry, Immunohistochemistry

Pricing

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Application Data (Sandwich ELISA analysis of CD178 binding using Mouse anti Human CD178 as a capture reagent and biotinylated Mouse anti Human CD178 as a detection reagent with purified human CD178 as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Plasma (orange) sample is displayed at 1:2 dilution.)

CD178, Monoclonal Antibody (Cat# AAA11962)

Full Name

MOUSE ANTI HUMAN CD178

Gene Names

FASLG; APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1

Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation

Pricing

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Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

Application Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12232)

Full Name

RAT ANTI TUBULIN ALPHA:HRP

Applications

Immunohistochemistry, Western Blot

Pricing

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Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

Application Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)