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Reactivity
Gene Name
Isotype
Host
Application
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5246 results for " Immunohistochemistry" - showing 4350-4400
WB (Western Blot)
(Western blot analysis of extracts from A431, using Phospho-SHP-2 (Tyr62) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
SHP-2, Polyclonal Antibody (Cat# AAA31155)
Full Name
Phospho-SHP-2 (Tyr62) Antibody
Gene Names
PTPN11; CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(CCR7 Antibody (N-term) (AAA28701) flow cytometric analysis of 293 cells (right histogram) compared to a negative controlcell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
CCR7, Polyclonal Antibody (Cat# AAA28701)
Full Name
CCR7 Antibody (N-term)
Gene Names
CCR7; BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(AAA30956 staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA30956 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
MITF, Polyclonal Antibody (Cat# AAA30956)
Full Name
Phospho-MITF (Ser180/73) Antibody
Gene Names
MITF; MI; WS2; CMM8; WS2A; COMMAD; bHLHe32
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity puriification
Pricing
IHC (Immunohistochemistry)
(Western Blot;Sample: Mouse Small intestine lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse DEFa1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Western Blot))
Defensin Alpha 1, Polyclonal Antibody (Cat# AAA20957)
Full Name
Polyclonal Antibody to Defensin Alpha 1, Neutrophil (DEFa1)
Gene Names
Defa1; Defcr; Defcr1
Reactivity
Mouse
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IHC (Immunohistochemistry)
(IHC (1:4000, 200X) analysis of p16 expression in HCC with Anti-p16 (AAA17979))
p16, Monoclonal Antibody (Cat# AAA17979)
Full Name
p16 Antibody
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Applications
Immunohistochemistry
Pricing
Cross Linked C-Telopeptide Of Type I Collagen (CTXI), Monoclonal Antibody (Cat# AAA21150)
Full Name
Monoclonal Antibody to Cross Linked C-Telopeptide Of Type I Collagen (CTXI)
Reactivity
Human, Dog, Bovine, Chicken
Applications
Western blotting: 0.01-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Purity
Protein A + Protein G affinity chromatography
Pricing
p16 - ink4a, Monoclonal Antibody (Cat# AAA13606)
Full Name
p16 - ink4a
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Protein A/G Chromatography
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue AAA29660 using at dilution 1/40.)
PRKD1, Polyclonal Antibody (Cat# AAA29660)
Full Name
PRKD1 Antibody
Gene Names
PRKD1; PKD; PKCM; PRKCM; PKC-MU
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-PSamples: Mouse Lung TissuePrimary Ab: 10ug/ml Rabbit Anti-MousePLA2R1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry))
Phospholipase A2 Receptor 1, Polyclonal Antibody (Cat# AAA20865)
Full Name
Polyclonal Antibody to Phospholipase A2 Receptor 1 (PLA2R1)
Gene Names
Pla2r1; Pla2r; Pla2-r; Pla2g1br
Reactivity
Mouse
Applications
WB, IHC, ICC, IP
Purity
Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
WB (Western Blot)
(Western blot analysis of various lysates, using NACA Rabbit pAb (AAA28217) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.)
NACA, Antibody (Cat# AAA28217)
Full Name
NACA Polyclonal Antibody
Gene Names
NACA; HSD48; NACA1; skNAC; NAC-alpha
Applications
Western Blot
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded colon cancer tissues using ASS1 mouse mAb with DAB staining.)
ASS1, Monoclonal Antibody (Cat# AAA14112)
Full Name
Anti-ASS1 Mouse mAb
Gene Names
ASS1; ASS; CTLN1
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Pricing
b2-Microglobulin, Monoclonal Antibody (Cat# AAA14654)
Full Name
b2-Microglobulin (B2M)
Gene Names
HLA-G; MHC-G
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence
Purity
Purified
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of Filaggrin staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Filaggrin, Polyclonal Antibody (Cat# AAA27815)
Full Name
Anti-Filaggrin Antibody
Gene Names
FLG; ATOD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
Application Data
(The indicated His-PGK1 proteins immobilized on Ni-NTAagarose beads was incubated with DYKDDDDK-ARD1 and Ac-CoA, followed by incubation with purified GST-Beclin1 or GST-Beclin1 S30A, inthe presence of [γ-32P] ATP. Autoradiography was performed.)
Beclin1 (Phospho-Ser30), Polyclonal Antibody (Cat# AAA29760)
Full Name
Beclin1 (Phospho-Ser30) Antibody
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of ZNT1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
ZNT1, Polyclonal Antibody (Cat# AAA27809)
Full Name
Anti-ZNT1 Antibody
Gene Names
SLC30A1; ZNT1; ZRC1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by affinity chromatography.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TWIST1 mouse mAb with DAB staining.)
TWIST1, Monoclonal Antibody (Cat# AAA14132)
Full Name
Anti-TWIST1 Mouse mAb
Gene Names
TWIST1; CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry
Pricing
IHC (Immunohistochemistry)
(Anti-beta-Actin antibody (monoclonal), AAA11508, IHC(P)IHC(P): Human Mammary Cancer Tissue)
beta-Actin, Monoclonal Antibody (Cat# AAA11508)
Full Name
Anti-beta-Actin antibody (monoclonal)
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Rat, Pig, Monkey, Rabbit, Chicken
Applications
Western Blot, Immunohistochemistry
Pricing
FCM (Flow Cytometry)
(Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 ug/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.)
TIGIT, Monoclonal Antibody (Cat# AAA11005)
Full Name
TIGIT Antibody [4A11]
Gene Names
TIGIT; VSIG9; VSTM3; WUCAM
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Protein A purified
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA28788 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28788, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was Rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
IDUA, Polyclonal Antibody (Cat# AAA28788)
Full Name
IDUA Antibody (Center)
Gene Names
IDUA; IDA; MPS1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31243 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
ADCY10, Polyclonal Antibody (Cat# AAA31243)
Full Name
ADCY10 Antibody
Gene Names
ADCY10; SAC; HCA2; SACI; Sacy; hsAC; HEL-S-7a
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Bovine(88%), Sheep(88%), Rabbit(88%), Dog(88%)
Predicted Reactivity: Pig(88%), Bovine(88%), Sheep(88%), Rabbit(88%), Dog(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells with AAA26952 at 1:333, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
XKR8, Polyclonal Antibody (Cat# AAA26952)
Full Name
XKR8 Antibody
Gene Names
XKR8; XRG8; hXkr8
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
WB (Western Blot)
(Western blot analysis of FKHRL1 phosphorylation expression in serum treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
FKHRL1, Polyclonal Antibody (Cat# AAA30954)
Full Name
Phospho-FKHRL1 (Ser253) Antibody
Gene Names
FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31197 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31197 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
CXCL16, Polyclonal Antibody (Cat# AAA31197)
Full Name
CXCL16 Antibody
Gene Names
CXCL16; SRPSOX; CXCLG16; SR-PSOX
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(AAA31014 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Histone H2A.X, Polyclonal Antibody (Cat# AAA31014)
Full Name
Phospho-Histone H2A.X (Ser139) Antibody
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(AAA31110 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
CHSY1, Polyclonal Antibody (Cat# AAA31110)
Full Name
CHSY1 Antibody
Gene Names
CHSY1; CHSY; CSS1; TPBS; ChSy-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry)
(AAA30928 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Nrf2, Polyclonal Antibody (Cat# AAA30928)
Full Name
Nrf2 Antibody
Gene Names
NFE2L2; NRF2; HEBP1; IMDDHH
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
Application Data
(The cancer cell lines were stably expressing DYKDDDDK-tagged RIP3 and RIP expression was detected by anti-RIP3 antibodies (AAA10922) in RIP3-overexpressed cells.)
RIP3, Polyclonal Antibody (Cat# AAA10922)
Full Name
RIP3 Antibody
Gene Names
Ripk3; Rip3; AW107945; 2610528K09Rik
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
RIP3 Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
GPCR GPR43 / FFAR2, Polyclonal Antibody (Cat# AAA14408)
Full Name
GPCR GPR43 Antibody
Gene Names
FFAR2; FFA2R; GPR43
Reactivity
Human, Mouse, Rat
Applications
Dot Blot, Immunohistochemistry, Immunoprecipitation, Western Blot
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human testis tissue using AAA26950 at dilution of 1:100)
FLVCR2, Polyclonal Antibody (Cat# AAA26950)
Full Name
FLVCR2 Antibody
Gene Names
FLVCR2; CCT; EPV; PVHH; MFSD7C; C14orf58; FLVCRL14q
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohistochemistry-Paraffin)
(Immunohistochemical analysis of paraffin-embedded H. liver section using TYK2 Antibody (C-term) . AAA28807 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
TYK2, Polyclonal Antibody (Cat# AAA28807)
Full Name
TYK2 Antibody (C-term)
Gene Names
TYK2; JTK1; IMD35
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(Flow cytometry analysis of LIGHT overexpressing HEK293 cells using LIGHT antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: LIGHT overexpressing HEK293 cells.)
LIGHT, Monoclonal Antibody (Cat# AAA11009)
Full Name
LIGHT Antibody [7B9H9]
Gene Names
TNFSF14; LTg; CD258; HVEML; LIGHT
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Protein A purified
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of TLR2 in normal human prostate tissue using TLR2 antibody (Clone: ABM3A87) at 5 ug/ml.)
TLR2, Monoclonal Antibody (Cat# AAA14864)
Full Name
TLR2 Monoclonal Antibody
Gene Names
TLR2; TIL4; CD282
Reactivity
Mouse, Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot, Immunofluorescence
Purity
Protein G Chromatography
Pricing
IHC (Immunohistochemistry)
(ERV3 Antibody (C-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ERV3 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.)
ERV3, Polyclonal Antibody (Cat# AAA28716)
Full Name
ERV3 Antibody (C-Term)
Gene Names
ERV3-1; ERV3; ERVR; envR; ERV-R; HERVR; HERV-R
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(HMGB1 was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- HMGB1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
HMGB1, Polyclonal Antibody (Cat# AAA11677)
Full Name
Anti-HMGB1 Antibody
Gene Names
HMGB1; HMG1; HMG3; HMG-1; SBP-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
MOR1, Polyclonal Antibody (Cat# AAA14412)
Full Name
MOR1 Antibody
Gene Names
OPRM1; MOP; MOR; LMOR; MOR1; OPRM; M-OR-1
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Pricing
IHC (Immunohistchemistry-Paraffin)
(AAA31276 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
SCAMP2, Polyclonal Antibody (Cat# AAA31276)
Full Name
SCAMP2 Antibody
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Collagen 4 alpha 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Collagen 4 alpha 5, Polyclonal Antibody (Cat# AAA27790)
Full Name
Anti-Collagen 4 alpha 5 Antibody
Gene Names
COL4A5; ATS; ASLN; CA54
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohistochemistry)
(AAA13663 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
P2RX7/P2X7 receptor, Polyclonal Antibody (Cat# AAA13663)
Full Name
Goat anti-P2RX7/P2X7 receptor Antibody
Gene Names
P2RX7; P2X7
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Peptide ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistchemistry)
(Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.)
c-FOS, Antibody (Cat# AAA13673)
Full Name
Goat anti-c-FOS (aa283-295) Antibody
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
Applications
Peptide ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with NuMA Monoclonal Antibody (A73-B/D12))
Nuclear Mitotic Apparatus Protein (NuMA), Monoclonal Antibody (Cat# AAA13819)
Full Name
Nuclear Mitotic Apparatus Protein (NuMA) Mouse Monoclonal Antibody
Gene Names
NUMA1; NUMA; NMP-22
Reactivity
Human (Others not known)
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
WB (Western Blot)
(Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B)(N-term). U251 cells(right) were treated with Chloroquine (50 uM,16h). AAA28778 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).)
LC3, Polyclonal Antibody (Cat# AAA28778)
Full Name
LC3 Antibody (APG8B) (N-term)
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, rat (Predicted Reactivity: Bovine)
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western blot analysis of JunD phosphorylation expression in 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
JunD, Polyclonal Antibody (Cat# AAA31019)
Full Name
Phospho-JunD (Ser255) Antibody
Gene Names
JUND; AP-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31224 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31224 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
CASC5, Polyclonal Antibody (Cat# AAA31224)
Full Name
CASC5 Antibody
Gene Names
KNL1; D40; CT29; Spc7; CASC5; MCPH4; hKNL-1; AF15Q14; PPP1R55; hSpc105
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Horse(88%), Rabbit(86%)
Predicted Reactivity: Pig(88%), Horse(88%), Rabbit(86%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA29758 staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
STING, Polyclonal Antibody (Cat# AAA29758)
Full Name
STING (Phospho-Ser366) Antibody
Gene Names
TMEM173; ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING
Reactivity
Human, Mouse
Applications
Westen Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of EGFR phosphorylation expression in 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
EGFR, Polyclonal Antibody (Cat# AAA30962)
Full Name
Phospho-EGFR (Ser695) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(Western blot analysis of recombinant EYFP protein with AAA27910 diluted at 1:10,000.)
EYFP, Monoclonal Antibody (Cat# AAA27910)
Full Name
EYFP Mouse Monoclonal Antibody (Mix-mA)
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Pricing
WB (Western Blot)
(Jurkat cells were subjected to SDS PAGE followed by western blot with AAA27492 (SELP Antibody) at dilution of 1:500)
P-selectin, Monoclonal Antibody (Cat# AAA27492)
Full Name
P-selectin Mouse Monoclonal
Gene Names
SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot, Immunoprecipitation
Purity
>95% as determined by SDS-PAGE
Protein A+G purification
Protein A+G purification
Pricing
IHC (Immunohistochemistry)
(Anti-IL-25 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IL25 / IL17E, Monoclonal Antibody (Cat# AAA12340)
Full Name
Mouse Monoclonal [clone 68C1039.2] (IgG1) to Human IL25 / IL17E
Gene Names
IL25; IL17E
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Protein G Purified
Pricing
IHC (Immunohistochemistry)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Liver X Receptor Beta, Antibody (Cat# AAA21042)
Full Name
Polyclonal Antibody to Liver X Receptor Beta (LXRb)
Gene Names
Nr1h2; UR; LXR; Unr; LXRB; NER1; OR-1; Unr2; RIP15; LXRBSV; LXRbeta; AI194859
Reactivity
Mouse, Human, Rat, Pig
Applications
Westen Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human small intestine using AAA26955 at dilution 1:100)
PPM1D, Polyclonal Antibody (Cat# AAA26955)
Full Name
PPM1D Antibody
Gene Names
PPM1D; WIP1; PP2C-DELTA
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing