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Reactivity
Gene Name
Isotype
Host
Application
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370 results for "Normal Serum" - showing 350-370
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U251 cells using anti-PNP antibody (AAA11680).Overlay histogram showing U251 cells stained with AAA11680 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNP Antibody (AAA11680,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PNP, Polyclonal Antibody (Cat# AAA11680)
Full Name
Anti-PNP Antibody
Gene Names
PNP; NP; PUNP; PRO1837
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of p53 phosphorylation expression in HU treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
p53, Polyclonal Antibody (Cat# AAA30977)
Full Name
Phospho-p53 (Ser15) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
PTH [1-34] (Human) IgG fraction, Polyclonal Antibody (Cat# AAA13481)
Full Name
PTH [1-34] (Human) IgG fraction
Applications
Immunocytochemistry, Western Blot
Pricing
IF (Immunofluorescence)
(AAA31211 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31211 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
SEPT4, Polyclonal Antibody (Cat# AAA31211)
Full Name
SEPT4 Antibody
Gene Names
SEPTIN4; H5; ARTS; MART; SEP4; CE5B3; SEPT4; PNUTL2; hucep-7; BRADEION; C17orf47; hCDCREL-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using Phospho-PDK1 (Ser241) Antibody.)
PDK1, Polyclonal Antibody (Cat# AAA30952)
Full Name
Phospho-PDK1 (Ser241) Antibody
Gene Names
PDPK1; PDK1; PDPK2; PDPK2P; PRO0461
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing U87 cells stained with AAA28063 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
CD9, Monoclonal Antibody (Cat# AAA28063)
Full Name
CD9 Monoclonal Antibody
Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
>95%, Protein G purified
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HL-60 cells using anti-METTL3 antibody (AAA19247).Overlay histogram showing HL-60 cells stained with AAA19247 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-METTL3 Antibody (AAA19247, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
METTL3, Polyclonal Antibody (Cat# AAA19247)
Full Name
Anti-METTL3 Antibody
Gene Names
METTL3; M6A; IME4; Spo8; MT-A70
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(AAA30967 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Lamin A/C, Polyclonal Antibody (Cat# AAA30967)
Full Name
Phospho-Lamin A/C (Ser392) Antibody
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
HIV1 p24, Recombinant Protein (Cat# AAA14383)
Full Name
HIV1 p24 protein
Purity
> 90% pure by SDS-PAGE
Purification: Agrose Chromatography
Purification: Agrose Chromatography
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of Hela cells using anti-Calbindin antibody (AAA11603).Overlay histogram showing Hela cells stained with AAA11603 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calbindin Antibody (AAA11603,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Calbindin, Polyclonal Antibody (Cat# AAA11603)
Full Name
Anti-Calbindin Antibody
Gene Names
CALB1; CALB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
SDS-PAGE
Monkey IgG (whole molecule), Immunoglobulin (Cat# AAA14398)
Full Name
Monkey IgG (whole molecule)
Applications
Immunohistochemistry, Western Blot
Purity
Monkey IgG (whole molecule) was purified by delipidation, salt fractionation and ion exchange chromatography.
Pricing
Application Data
CEA, Native Protein (Cat# AAA14367)
Full Name
CEA protein
Gene Names
CEACAM6; NCA; CEAL; CD66c
Applications
Control
Purity
>95% by SDS-PAGE and electrophoresis
Pricing
IA (Immuno Assay)
(used as capture antibody. Different concentrations were tested (0.5-2 ug/ml).)
Calprotectin, Monoclonal Antibody (Cat# AAA14609)
Full Name
Calprotectin, Human, mAb 27E10, Biotin
Reactivity
Mouse: No; Rhesus Monkey: Yes (subpopulations of macrophages)
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoassay, Immunoprecipitation, Western Blot
Purity
Protein G
Pricing
Rabbit anti Monkey IgG Fab, Polyclonal Secondary Antibody (Cat# AAA14570)
Full Name
Rabbit anti Monkey IgG Fab
Reactivity
Monkey
Applications
Immunofluorescence, Dot Blot, Immunoblot
Purity
Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of K562 cells using anti-CASP3 antibody (AAA11633).Overlay histogram showing K562 cells stained with AAA11633 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CASP3 Antibody (AAA11633,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Caspase-3, Polyclonal Antibody (Cat# AAA11633)
Full Name
Anti-Caspase-3 Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
Alpha Fetoprotein, Monoclonal Antibody (Cat# AAA14623)
Full Name
mAb anti-Human Alpha Fetoprotein
Gene Names
AFP; AFPD; FETA; HPAFP
Reactivity
Human. Others not tested
Applications
Western Blot
Purity
Protein G affinity purified
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U87 cells using anti-JUNB antibody (AAA19249)Overlay histogram showing U87 cells stained with AAA19249 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JUNB Antibody (AAA19249, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
JUNB, Polyclonal Antibody (Cat# AAA19249)
Full Name
Anti-JUNB Antibody
Gene Names
JUNB; AP-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of C6 cells using anti-KCNQ2 antibody. Overlay histogram showing C6 cells stained with (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNQ2 Antibody (1 ug/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
KCNQ2, Polyclonal Antibody (Cat# AAA19238)
Full Name
Anti-KCNQ2 Antibody
Gene Names
KCNQ2; EBN; BFNC; EBN1; ENB1; BFNS1; EIEE7; HNSPC; KV7.2; KCNA11; KVEBN1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of METTL3 using anti-METTL3 antibody (AAA19248).METTL3 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL3 Antibody (AAA19248) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
METTL3, Polyclonal Antibody (Cat# AAA19248)
Full Name
Anti-METTL3 Antibody
Gene Names
METTL3; M6A; IME4; Spo8; MT-A70
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
Serum, Animal Biological Fluid (Cat# AAA14387)
Full Name
Normal Donkey Serum
Reactivity
Donkey
Pricing