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368 results for " Isotype control" - showing 350-368
WB (Western Blot)
(All lanes : Anti-LAG3 Antibody (Center) at 1:1000 dilutionLane 1: human ovary lysateLane 2: mouse liver lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 57 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
LAG3, Polyclonal Antibody (Cat# AAA28766)
Full Name
LAG3 Antibody (Center)
Gene Names
LAG3; CD223
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of Hela cells using anti-Calbindin antibody (AAA11603).Overlay histogram showing Hela cells stained with AAA11603 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calbindin Antibody (AAA11603,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Calbindin, Polyclonal Antibody (Cat# AAA11603)
Full Name
Anti-Calbindin Antibody
Gene Names
CALB1; CALB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
CD45, Monoclonal Antibody (Cat# AAA14270)
Full Name
Anti-Mouse CD45, Purified (Clone IBL-5/25) (rat IgG1)
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
Purified from ascitic fluid via Protein G Chromatography
Pricing
IA (Immuno Assay)
(used as capture antibody. Different concentrations were tested (0.5-2 ug/ml).)
Calprotectin, Monoclonal Antibody (Cat# AAA14609)
Full Name
Calprotectin, Human, mAb 27E10, Biotin
Reactivity
Mouse: No; Rhesus Monkey: Yes (subpopulations of macrophages)
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoassay, Immunoprecipitation, Western Blot
Purity
Protein G
Pricing
FCM (Flow Cytometry)
(Flow Cytometry analyis of SSTR2 in human cell line. Human breast cancer cell line was stained with AAA14651 (filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 secondary antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.)
Somatostatin Receptor Type 2, Monoclonal Antibody (Cat# AAA14651)
Full Name
Somatostatin Receptor Type 2 (SSTr2)
Gene Names
SSTR2; SSTR2
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Flow Cytometry
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Pricing
WB (Western Blot)
(Western Blot Analysis of human Small Intestine tissue lysate using CDH17 Mouse Monoclonal Antibody (CDH17/2618).)
Cadherin 17/LI Cadherin, Monoclonal Antibody (Cat# AAA23911)
Full Name
Cadherin 17/LI Cadherin (Liver-Intestine Marker)
Gene Names
CDH17; HPT1; CDH16; HPT-1
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of K562 cells using anti-CASP3 antibody (AAA11633).Overlay histogram showing K562 cells stained with AAA11633 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CASP3 Antibody (AAA11633,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Caspase-3, Polyclonal Antibody (Cat# AAA11633)
Full Name
Anti-Caspase-3 Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(SCNN1A Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SCNN1A, Polyclonal Antibody (Cat# AAA28784)
Full Name
SCNN1A Antibody (Center)
Gene Names
SCNN1A; BESC2; ENaCa; SCNEA; SCNN1; ENaCalpha
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Figure-4-Cell surface flow analysis of Rabbit IgG Isotype control in 3T3 Cell line using 0.5/10^6 cells. Black represent cell alone, grey represent secondary antibody control and orange represent Rabbit IgG Isotype with anti-rabbit secondary antibody conjugated with PE (Cat No. AAA14878).)
IgG, Polyclonal Isotype Control (Cat# AAA14878)
Full Name
Rabbit IgG Isotype Control
Applications
Flow Cytometry
Purity
Affinity Chromatography
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Annexin A1, Monoclonal Antibody (Cat# AAA23918)
Full Name
Annexin A1/(Hairy Cell Leukemia Marker)
Gene Names
ANXA1; ANX1; LPC1
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing HeLa cells stained with AAA28782(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28782, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
HNRNPR, Polyclonal Antibody (Cat# AAA28782)
Full Name
HNRNPR Antibody (N-term)
Gene Names
HNRNPR; HNRPR; hnRNP-R
Reactivity
Human
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescence image of HeLa Cells using S100A4 Mouse Monoclonal Antibody (S100A4/1482) followed by goat anti-mouse IgG-CF488 (green). Reddot is used to label the nuclei Red.)
S100A4, Monoclonal Antibody (Cat# AAA23892)
Full Name
S100A4 (Marker of Tumor Metastasis)
Gene Names
S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Laminin Receptor/RPSA, Monoclonal Antibody (Cat# AAA23908)
Full Name
Laminin Receptor/RPSA (Marker of Metastatic Potential)
Gene Names
RPSA; SA; LBP; LRP; p40; 67LR; ICAS; lamR; 37LRP; LAMBR; LAMR1; LRP/LR; LBP/p40; NEM/1CHD4
Reactivity
Human. Others not known.
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U87 cells using anti-JUNB antibody (AAA19249)Overlay histogram showing U87 cells stained with AAA19249 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JUNB Antibody (AAA19249, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
JUNB, Polyclonal Antibody (Cat# AAA19249)
Full Name
Anti-JUNB Antibody
Gene Names
JUNB; AP-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Staining of C57BL/6 splenocytes with 0.25 ug of Mouse IgG2a kappa Isotype Control (FITC) (open histogram) or 0.25 ug of Qa-2 antibody (FITC) (filled histogram). Total viable cells were used for analysis.)
Qa2, Monoclonal Antibody (Cat# AAA17929)
Full Name
Qa2 antibody (FITC)
Gene Names
H2-Q7; Q9; Ped; Qa7; Qa-2; Qa-7; H-2Q7
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of C6 cells using anti-KCNQ2 antibody. Overlay histogram showing C6 cells stained with (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNQ2 Antibody (1 ug/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
KCNQ2, Polyclonal Antibody (Cat# AAA19238)
Full Name
Anti-KCNQ2 Antibody
Gene Names
KCNQ2; EBN; BFNC; EBN1; ENB1; BFNS1; EIEE7; HNSPC; KV7.2; KCNA11; KVEBN1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of METTL3 using anti-METTL3 antibody (AAA19248).METTL3 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL3 Antibody (AAA19248) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
METTL3, Polyclonal Antibody (Cat# AAA19248)
Full Name
Anti-METTL3 Antibody
Gene Names
METTL3; M6A; IME4; Spo8; MT-A70
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
Application Data
(Flow Cytometry analysis of intracellular staining in mouse fibroblast cell line L929 using AAA14733 (filled histogram) or with isotype control (open histogram).)
Actin, alpha, Smooth Muscle, Monoclonal Antibody (Cat# AAA14733)
Full Name
Actin, alpha, Smooth Muscle (PE)
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Human
Applications
Flow Cytometry
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Pricing