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391 results for " Isotype" - showing 350-391
FCM (Flow Cytometry)
(Intracellular flow cytometric analysis of RIG-I in K562 Cell line using 0.5 ug/10^6 cells of Anti-RIGI antibody (ABM40B5). Green represent isotype control and red represent Anti-RIG I antibody (AAA14865). Goat anti-mouse PE conjugate was used as secondary.)
RIG-I, Monoclonal Antibody (Cat# AAA14865)
Full Name
RIG-I Monoclonal Antibody
Gene Names
DDX58; RIGI; RIG-I; RLR-1; SGMRT2
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
Protein G Chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescence Analysis of methanol-fixed human tonsil cryosection stained with CF488A CD3e clone RIV9 (green), mounted in EverBrite’ Mounting Medium with DAPI (nuclei, blue).)
CD3, Monoclonal Antibody (Cat# AAA13839)
Full Name
CD3 (T-Cell Marker) Mouse Monoclonal Antibody
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human, Mouse, Rat
Applications
Functional Assay, Flow Cytometry, Immunofluorescence
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating TUBB in Hela whole cell lysateLane 1: Mouse control IgG instead in Hela whole cell lysate.Lane 2: (2ug) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (5ug)For western blotting, the blot was detected at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:5000)
TUBB, Monoclonal Antibody (Cat# AAA28058)
Full Name
TUBB Monoclonal Antibody
Reactivity
Human, Rat, Rabbit, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein A purified
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA12029)
Full Name
MOUSE ANTI HUMAN CD105:RPE
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA12083)
Full Name
MOUSE ANTI HUMAN CD105:FITC
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA11865)
Full Name
MOUSE ANTI HUMAN CD105:FITC
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(Intracellular flow cytometric analysis of RIG-I in K562 Cell line using 0.5 ug/10^6 cells of Anti-RIGI antibody (ABM4H29). Green represent isotype control and red represent Anti-RIG I antibody (AAA14869). Goat anti-mouse PE conjugate was used as secondary.)
RIG I /DDx58, Monoclonal Antibody (Cat# AAA14869)
Full Name
Monoclonal Antibody to RIG-I (Clone: ABM4H29)
Gene Names
DDX58; RIGI; RIG-I; RLR-1; SGMRT2
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
Protein G chromatography
Pricing
WB (Western Blot)
(Figure-6: Western blot analysis of Caspase-8. Anti-Caspase-8 antibody (Clone: ABM14C1) was used at 4 ug/ml on EL-4 lysate.)
Caspase-8, Monoclonal Antibody (Cat# AAA14877)
Full Name
Monoclonal Antibody to Caspase-8 (Clone: ABM14C1)
Gene Names
CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry
Purity
Purified
Protein G Chromatography
Protein G Chromatography
Pricing
FCM (Flow Cytometry)
(Flow Cytometry detection of FOLR1 in MCF-7 human cell line using AAA14691. MCF-7 human breast cancer cell line was stained with AAA14691 (filled histogram) or isotype control antibody (open histogram), followed byPhycoerythrin-conjugated Anti-MouseIgG Secondary Antibody.)
FOLR1, Monoclonal Antibody (Cat# AAA14691)
Full Name
FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)
Gene Names
FOLR1; FBP; FOLR
Reactivity
Human
Applications
Flow Cytometry, Western Blot, Immunocytochemistry
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of Dectin-2 in Renal Cell Carcinoma using Dectin-2 antibody (Clone: ABM2H28) at 5 ug/ml.)
Dectin-2, Monoclonal Antibody (Cat# AAA14862)
Full Name
Dectin-2 Monoclonal Antibody
Gene Names
CLEC6A; CLEC4N; CLECSF10
Reactivity
Human, Mouse
Applications
Western Blot, Flow Cytometry, Immunohistochemistry
Purity
Protein G Chromatography
Pricing
FCM (Flow Cytometry)
(Flow Cytometric Analysis of Human Raji cells using HLA-DP (MHC II) Monoclonal Antibody (SPM421) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).)
HLA-DP, Antibody (Cat# AAA13830)
Full Name
HLA-DP (MHC II) Mouse Monoclonal Antibody
Gene Names
HLA-DPB1; DPB1; HLA-DP; HLA-DPB; HLA-DP1B
Reactivity
Human. Others not known.
Applications
Flow Cytometry, Immunofluorescence
Pricing
IHC (Immunohistochemistry)
(Western blot analysis of lysates from 293T cell line human brain and mouse brain tissue lysate(from left to right), using STRADA Antibody (C-term). AAA28673 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
STRADA, Polyclonal Antibody (Cat# AAA28673)
Full Name
STRADA Antibody (C-term)
Gene Names
STRADA; LYK5; PMSE; Stlk; STRAD; NY-BR-96
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Applications
Western Blot, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U20S cells using anti-Calpastatin antibody (AAA11679).Overlay histogram showing U20S cells stained with AAA11679 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calpastatin Antibody (AAA11679,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Calpastatin, Polyclonal Antibody (Cat# AAA11679)
Full Name
Anti-Calpastatin Antibody
Gene Names
CAST; BS-17; PLACK
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U251 cells using anti-PNP antibody (AAA11680).Overlay histogram showing U251 cells stained with AAA11680 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNP Antibody (AAA11680,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PNP, Polyclonal Antibody (Cat# AAA11680)
Full Name
Anti-PNP Antibody
Gene Names
PNP; NP; PUNP; PRO1837
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IgG, Polyclonal Secondary Antibody (Cat# AAA14879)
Full Name
APC conjugated Goat anti Rabbit IgG (H+L)
Applications
Immunofluorescence, Flow Cytometry
Purity
Affinity Chromatography
Pricing
Infectious bronchitis virus, Monoclonal Antibody (Cat# AAA14852)
Full Name
Monoclonal mouse anti-infectious bronchitis virus (IBV)
Applications
Western Blot
Purity
Protein A chromatography
Pricing
FCM (Flow Cytometry)
(Flow Cytometric Analysis of PFA-fixed Raji cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).)
Human Nuclear Antigen, Monoclonal Antibody (Cat# AAA13804)
Full Name
Human Nuclear Antigen
Reactivity
Human and Non-human primates. Does not react with mouse, rat, and chicken. Others not known.
Applications
Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry
Pricing
Application Data
(Overlay histogram showing U-2OS cells stained with AP10510c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated n 2% bovine serum albumin to block non-specificprotein-protein interactions followed by the antibody (AP10510c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used wasGoat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events wasperformed)
CF150, Polyclonal Antibody (Cat# AAA28789)
Full Name
CF150 Antibody (Center)
Gene Names
MB21D1; cGAS; h-cGAS; C6orf150
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immmunohistochemistry)
(PLA2G7 Antibody (Center) (Cat. #AAA28698)IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PLA2G7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
PLA2G7, Polyclonal Antibody (Cat# AAA28698)
Full Name
PLA2G7 Antibody (Center)
Gene Names
PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing U87 cells stained with AAA28063 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
CD9, Monoclonal Antibody (Cat# AAA28063)
Full Name
CD9 Monoclonal Antibody
Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
>95%, Protein G purified
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HL-60 cells using anti-METTL3 antibody (AAA19247).Overlay histogram showing HL-60 cells stained with AAA19247 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-METTL3 Antibody (AAA19247, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
METTL3, Polyclonal Antibody (Cat# AAA19247)
Full Name
Anti-METTL3 Antibody
Gene Names
METTL3; M6A; IME4; Spo8; MT-A70
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of Hela cells using anti-Calbindin antibody (AAA11603).Overlay histogram showing Hela cells stained with AAA11603 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calbindin Antibody (AAA11603,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Calbindin, Polyclonal Antibody (Cat# AAA11603)
Full Name
Anti-Calbindin Antibody
Gene Names
CALB1; CALB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(All lanes : Anti-LAG3 Antibody (Center) at 1:1000 dilutionLane 1: human ovary lysateLane 2: mouse liver lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 57 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
LAG3, Polyclonal Antibody (Cat# AAA28766)
Full Name
LAG3 Antibody (Center)
Gene Names
LAG3; CD223
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
CD45, Monoclonal Antibody (Cat# AAA14270)
Full Name
Anti-Mouse CD45, Purified (Clone IBL-5/25) (rat IgG1)
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
Purified from ascitic fluid via Protein G Chromatography
Pricing
IA (Immuno Assay)
(used as capture antibody. Different concentrations were tested (0.5-2 ug/ml).)
Calprotectin, Monoclonal Antibody (Cat# AAA14609)
Full Name
Calprotectin, Human, mAb 27E10, Biotin
Reactivity
Mouse: No; Rhesus Monkey: Yes (subpopulations of macrophages)
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoassay, Immunoprecipitation, Western Blot
Purity
Protein G
Pricing
Rabbit anti Monkey IgG Fab, Polyclonal Secondary Antibody (Cat# AAA14570)
Full Name
Rabbit anti Monkey IgG Fab
Reactivity
Monkey
Applications
Immunofluorescence, Dot Blot, Immunoblot
Purity
Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Pricing
FCM (Flow Cytometry)
(Flow Cytometry analyis of SSTR2 in human cell line. Human breast cancer cell line was stained with AAA14651 (filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 secondary antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.)
Somatostatin Receptor Type 2, Monoclonal Antibody (Cat# AAA14651)
Full Name
Somatostatin Receptor Type 2 (SSTr2)
Gene Names
SSTR2; SSTR2
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Flow Cytometry
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Pricing
WB (Western Blot)
(Western Blot Analysis of human Small Intestine tissue lysate using CDH17 Mouse Monoclonal Antibody (CDH17/2618).)
Cadherin 17/LI Cadherin, Monoclonal Antibody (Cat# AAA23911)
Full Name
Cadherin 17/LI Cadherin (Liver-Intestine Marker)
Gene Names
CDH17; HPT1; CDH16; HPT-1
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of K562 cells using anti-CASP3 antibody (AAA11633).Overlay histogram showing K562 cells stained with AAA11633 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CASP3 Antibody (AAA11633,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Caspase-3, Polyclonal Antibody (Cat# AAA11633)
Full Name
Anti-Caspase-3 Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(SCNN1A Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SCNN1A, Polyclonal Antibody (Cat# AAA28784)
Full Name
SCNN1A Antibody (Center)
Gene Names
SCNN1A; BESC2; ENaCa; SCNEA; SCNN1; ENaCalpha
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Figure-4-Cell surface flow analysis of Rabbit IgG Isotype control in 3T3 Cell line using 0.5/10^6 cells. Black represent cell alone, grey represent secondary antibody control and orange represent Rabbit IgG Isotype with anti-rabbit secondary antibody conjugated with PE (Cat No. AAA14878).)
IgG, Polyclonal Isotype Control (Cat# AAA14878)
Full Name
Rabbit IgG Isotype Control
Applications
Flow Cytometry
Purity
Affinity Chromatography
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Annexin A1, Monoclonal Antibody (Cat# AAA23918)
Full Name
Annexin A1/(Hairy Cell Leukemia Marker)
Gene Names
ANXA1; ANX1; LPC1
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing HeLa cells stained with AAA28782(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28782, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
HNRNPR, Polyclonal Antibody (Cat# AAA28782)
Full Name
HNRNPR Antibody (N-term)
Gene Names
HNRNPR; HNRPR; hnRNP-R
Reactivity
Human
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescence image of HeLa Cells using S100A4 Mouse Monoclonal Antibody (S100A4/1482) followed by goat anti-mouse IgG-CF488 (green). Reddot is used to label the nuclei Red.)
S100A4, Monoclonal Antibody (Cat# AAA23892)
Full Name
S100A4 (Marker of Tumor Metastasis)
Gene Names
S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
Cancer Antigen 19-9 (CA19-9), Monoclonal Antibody (Cat# AAA17954)
Full Name
Cancer Antigen 19-9 (CA19-9) Antibody-BIOTIN
Applications
Immunohistochemistry
Purity
Protein A chromatography
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Laminin Receptor/RPSA, Monoclonal Antibody (Cat# AAA23908)
Full Name
Laminin Receptor/RPSA (Marker of Metastatic Potential)
Gene Names
RPSA; SA; LBP; LRP; p40; 67LR; ICAS; lamR; 37LRP; LAMBR; LAMR1; LRP/LR; LBP/p40; NEM/1CHD4
Reactivity
Human. Others not known.
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U87 cells using anti-JUNB antibody (AAA19249)Overlay histogram showing U87 cells stained with AAA19249 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JUNB Antibody (AAA19249, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
JUNB, Polyclonal Antibody (Cat# AAA19249)
Full Name
Anti-JUNB Antibody
Gene Names
JUNB; AP-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Staining of C57BL/6 splenocytes with 0.25 ug of Mouse IgG2a kappa Isotype Control (FITC) (open histogram) or 0.25 ug of Qa-2 antibody (FITC) (filled histogram). Total viable cells were used for analysis.)
Qa2, Monoclonal Antibody (Cat# AAA17929)
Full Name
Qa2 antibody (FITC)
Gene Names
H2-Q7; Q9; Ped; Qa7; Qa-2; Qa-7; H-2Q7
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of C6 cells using anti-KCNQ2 antibody. Overlay histogram showing C6 cells stained with (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNQ2 Antibody (1 ug/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
KCNQ2, Polyclonal Antibody (Cat# AAA19238)
Full Name
Anti-KCNQ2 Antibody
Gene Names
KCNQ2; EBN; BFNC; EBN1; ENB1; BFNS1; EIEE7; HNSPC; KV7.2; KCNA11; KVEBN1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of METTL3 using anti-METTL3 antibody (AAA19248).METTL3 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL3 Antibody (AAA19248) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
METTL3, Polyclonal Antibody (Cat# AAA19248)
Full Name
Anti-METTL3 Antibody
Gene Names
METTL3; M6A; IME4; Spo8; MT-A70
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
Application Data
(Flow Cytometry analysis of intracellular staining in mouse fibroblast cell line L929 using AAA14733 (filled histogram) or with isotype control (open histogram).)
Actin, alpha, Smooth Muscle, Monoclonal Antibody (Cat# AAA14733)
Full Name
Actin, alpha, Smooth Muscle (PE)
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Human
Applications
Flow Cytometry
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
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