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1005 results for " HRP conjugated " - showing 350-400
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of A549 cells using anti-MCM5 antibody (AAA19377).Overlay histogram showing A549 cells stained with AAA19377 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- MCM5 Antibody (AAA19377, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MCM5, Monoclonal Antibody (Cat# AAA19377)
Full Name
Anti-MCM5 Antibody (monoclonal, 4G10)
Gene Names
MCM5; CDC46; P1-CDC46
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
SRPK2, Polyclonal Antibody (Cat# AAA31293)
Full Name
Phospho-SRPK2 (Ser494/Ser497) Antibody
Gene Names
SRPK2; SFRSK2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)
EOMES, Polyclonal Antibody (Cat# AAA26886)
Full Name
EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)
Gene Names
Eomes; Tbr2; TBR-2; C77258
Reactivity
Mouse, Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of U20S cells using anti-NFIA antibody (AAA11691).Overlay histogram showing U20S cells stained with AAA11691 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFIA Antibody (AAA11691,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NFIA, Polyclonal Antibody (Cat# AAA11691)
Full Name
Anti-NFIA Antibody
Gene Names
NFIA; CTF; NF1-A; NFI-A; NFI-L; NF-I/A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution of AAA14728. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit . Immunoreactivity seen here is mostly nuclear.)
EOMES, Polyclonal Antibody (Cat# AAA14728)
Full Name
EOMES, NT (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)
Gene Names
EOMES; TBR2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U20S cells using anti-PPID antibody (AAA19159).Overlay histogram showing U20S cells stained with AAA19159 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPID Antibody (AAA19159,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PPID/Cyclophilin 40, Polyclonal Antibody (Cat# AAA19159)
Full Name
Anti-PPID/Cyclophilin 40 Picoband antibody
Gene Names
PPID; CYPD; CYP-40
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HepG2 cells using anti-MERTK antibody (AAA19219).Overlay histogram showing HepG2 cells stained with AAA19219 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MERTK Antibody (AAA19219, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MERTK, Polyclonal Antibody (Cat# AAA19219)
Full Name
Anti-MERTK Antibody
Gene Names
MERTK; MER; RP38; c-mer
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of GALNS using anti-GALNS antibody (AAA19164).GALNS was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GALNS Antibody (AAA19164) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
GALNS, Antibody (Cat# AAA19164)
Full Name
Anti-GALNS Picoband antibody
Gene Names
GALNS; GAS; MPS4A; GalN6S; GALNAC6S
Reactivity
Reacts with: Human, Mouse, Rat
Applications
WB, EIA
Purity
Immunogen affinity purified
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HEPA1-6 cells using anti-Rad9b antibody (AAA19340).Overlay histogram showing HEPA1-6 cells stained with AAA19340 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rad9b Antibody (AAA19340, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rad9b, Polyclonal Antibody (Cat# AAA19340)
Full Name
Anti-Rad9b Antibody
Gene Names
Rad9b; BC021784; A630082N15Rik
Reactivity
Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells with AAA27002 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
SLC26A4, Polyclonal Antibody (Cat# AAA27002)
Full Name
SLC26A4 Antibody
Gene Names
SLC26A4; EVA; PDS; DFNB4; TDH2B
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
JAK3, Polyclonal Antibody (Cat# AAA31405)
Full Name
Phospho-JAK3 (Tyr981) Antibody
Gene Names
JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (100%), Bovine (91%), Horse (100%), Sheep (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (91%), Horse (100%), Sheep (100%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Figure 6. IF analysis of UNG using anti-UNG antibody (AAA19245).UNG was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- UNG Antibody (AAA19245) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
UNG, Polyclonal Antibody (Cat# AAA19245)
Full Name
Anti-UNG Antibody
Gene Names
UNG; DGU; UDG; UNG1; UNG2; HIGM4; HIGM5; UNG15
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence - anti-mScarlet Ab using hCEC cells transduced with mScarlet-Rab5a; cells were fixed with methanol and anti-mScarlet at 1/250;)
mScarlet, Polyclonal Antibody (Cat# AAA13885)
Full Name
anti-mScarlet
Reactivity
Reacts with Transfected cells proteins
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Immunoelectron Microscopy
Purity
This antibody is epitope-affinity purified from goat antiserum.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of U87 cells using anti-Transketolase/TKT antibody (AAA19254).Overlay histogram showing U87 cells stained with AAA19254 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Transketolase/TKT Antibody (AAA19254,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Transketolase/TKT, Polyclonal Antibody (Cat# AAA19254)
Full Name
Anti-Transketolase/TKT Antibody
Gene Names
TKT; TK; TKT1; HEL107
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(AAA30925 at 1/100 staining human bone tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
MCM5, Polyclonal Antibody (Cat# AAA30925)
Full Name
MCM5 Antibody
Gene Names
MCM5; CDC46; MGORS8; P1-CDC46
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
MLC2, Polyclonal Antibody (Cat# AAA31471)
Full Name
Phospho-MLC2 (Ser15) Antibody
Gene Names
MYL2; MLC2; CMH10
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Predicted Reactivity: Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of Integrin beta 4/ITGB4 using anti-Integrin beta 4/ITGB4 antibody (AAA19269).Integrin beta 4/ITGB4 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Integrin beta 4/ITGB4 Antibody (AAA19269) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
mGluR1/GRM1, Polyclonal Antibody (Cat# AAA19269)
Full Name
Anti-mGluR1/GRM1 Antibody
Gene Names
GRM1; MGLU1; GPRC1A; MGLUR1; SCAR13; PPP1R85
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HL-60 cells using anti-REA/PHB2 antibody (AAA19273).Overlay histogram showing HL-60 cells stained with AAA19273 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-REA/PHB2 Antibody (AAA19273,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
REA/PHB2, Polyclonal Antibody (Cat# AAA19273)
Full Name
Anti-REA/PHB2 Antibody
Gene Names
PHB2; BAP; REA; p22; Bap37; BCAP37; PNAS-141
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-Claudin 3/CLDN3 antibody (AAA19294).Overlay histogram showing MCF-7 cells stained with AAA19294 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Claudin 3/CLDN3 Antibody (AAA19294, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Claudin 3/CLDN3, Polyclonal Antibody (Cat# AAA19294)
Full Name
Anti-Claudin 3/CLDN3 Antibody
Gene Names
CLDN3; RVP1; HRVP1; C7orf1; CPE-R2; CPETR2
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
Application Data
(Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Medium power)
CD3, Monoclonal Antibody (Cat# AAA11839)
Full Name
MOUSE ANTI HUMAN CD3:APC
Gene Names
CD3G; T3G; IMD17; CD3-GAMMA
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Ntera-2 cells stained with CSB-MA888008A0m (red line) at 1:250. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
NANOG, Monoclonal Antibody (Cat# AAA27018)
Full Name
NANOG Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
>95%
Protein G Purified
Protein G Purified
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Smad5, Polyclonal Antibody (Cat# AAA31331)
Full Name
Phospho-Smad5 (Ser463/Ser465) Antibody
Gene Names
SMAD5; DWFC; JV5-1; MADH5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of MCF-7 cells using anti- PCK2 antibody (AAA19384).Overlay histogram showing MCF-7 cells stained with AAA19384 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PCK2 Antibody (AAA19384, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PCK2, Monoclonal Antibody (Cat# AAA19384)
Full Name
Anti-PCK2 Antibody (monoclonal, 3F7)
Gene Names
PCK2; PEPCK; PEPCK2; PEPCK-M
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of HL-60 cells using anti-NDUFB10 antibody (AAA19339).Overlay histogram showing HL-60 cells stained with AAA19339 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFB10 Antibody (AAA19339, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NDUFB10, Polyclonal Antibody (Cat# AAA19339)
Full Name
Anti-NDUFB10 Antibody
Gene Names
NDUFB10; PDSW
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-AML1 (Ser397) in lysates of Jurkat, using Phospho-AML1 (Ser397) Antibody.)
AML1, Polyclonal Antibody (Cat# AAA31153)
Full Name
Phospho-AML1 (Ser397) Antibody
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Figure 5. IHC analysis of GNG4 using anti-GNG4 antibody (AAA19341).GNG4 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG4 Antibody (AAA19341) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
GNG4, Polyclonal Antibody (Cat# AAA19341)
Full Name
Anti-GNG4 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-CHRNA5 antibody (AAA11685).Overlay histogram showing MCF-7 cells stained with AAA11685 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CHRNA5 Antibody (AAA11685,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CHRNA5, Polyclonal Antibody (Cat# AAA11685)
Full Name
Anti-CHRNA5 Antibody
Gene Names
CHRNA5; LNCR2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of A431 cells using anti-UBE2D1/2/3/4 antibody (AAA19298).Overlay histogram showing A431 cells stained with AAA19298 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2D1/2/3/4 Antibody (AAA19298, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
UBE2D1/2/3/4, Polyclonal Antibody (Cat# AAA19298)
Full Name
Anti-UBE2D1/2/3/4 Antibody
Gene Names
UBE2D1; SFT; UBCH5; UBC4/5; UBCH5A; E2(17)KB1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of THP-1 cells using anti-Ku70 antibody (AAA19362).Overlay histogram showing THP-1 cells stained with AAA19362 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Ku70 Antibody (AAA19362, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Ku70, Monoclonal Antibody (Cat# AAA19362)
Full Name
Anti-Ku70 Antibody (monoclonal, 3D7)
Gene Names
XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
FRS2, Polyclonal Antibody (Cat# AAA31412)
Full Name
Phospho-FRS2 (Tyr196) Antibody
Gene Names
FRS2; SNT; SNT1; FRS2A; SNT-1; FRS2alpha
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
HBP1, Polyclonal Antibody (Cat# AAA31326)
Full Name
Phospho-HBP1 (Ser402) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of anti-NEK2 Antibody (Center) in HL60 cell line lysates (35ug/lane). NEK2(arrow) was detected using the purified Pab.)
NEK2, Polyclonal Antibody (Cat# AAA28694)
Full Name
NEK2 Antibody (Center)
Gene Names
NEK2; NLK1; RP67; NEK2A; HsPK21; PPP1R111
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of A549 cells using anti-AGO3 antibody (AAA19290).Overlay histogram showing A549 cells stained with AAA19290 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AGO3 Antibody (AAA19290, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
AGO3, Polyclonal Antibody (Cat# AAA19290)
Full Name
Anti-AGO3 Antibody
Gene Names
AGO3; EIF2C3
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of K562 cells using anti-HNRNPH3 antibody (AAA19338).Overlay histogram showing K562 cells stained with AAA19338 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNRNPH3 Antibody (AAA19338, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HNRNPH3, Polyclonal Antibody (Cat# AAA19338)
Full Name
Anti-HNRNPH3 Antibody
Gene Names
HNRNPH3; 2H9; HNRPH3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
CUTL1, Polyclonal Antibody (Cat# AAA31424)
Full Name
Phospho-CUTL1 (Ser1237) Antibody
Gene Names
CUX1; CDP; CUX; p75; CASP; CDP1; COY1; Clox; p100; p110; p200; CUTL1; GOLIM6; CDP/Cut; Cux/CDP; Nbla10317
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(IHC image diluted at 1:1000 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
PKM, Monoclonal Antibody (Cat# AAA27048)
Full Name
PKM Monoclonal Antibody
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein G purified
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of Raji cells using anti-CD38 antibody (AAA19217).Overlay histogram showing Raji cells stained with AAA19217 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD38 Antibody (AAA19217, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CD38, Polyclonal Antibody (Cat# AAA19217)
Full Name
Anti-CD38 Antibody
Gene Names
CD38; T10; ADPRC 1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of RAW264. 7 cells using anti-TRIM25/EFP antibody (AAA19272).Overlay histogram showing RAW264. 7 cells stained with AAA19272 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM25/EFP Antibody (AAA19272, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TRIM25/EFP, Polyclonal Antibody (Cat# AAA19272)
Full Name
Anti-TRIM25/EFP Antibody
Gene Names
Trim25; EFP; Zfp147; AA960166; AL022677
Reactivity
Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
Application Data
(Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Medium power)
CD3, Monoclonal Antibody (Cat# AAA11974)
Full Name
MOUSE ANTI HUMAN CD3
Gene Names
CD3G; T3G; IMD17; CD3-GAMMA
Applications
Immunohistochemistry, Flow Cytometry
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of K562 cells using anti-IRF3 antibody (AAA19216).Overlay histogram showing K562 cells stained with AAA19216 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IRF3 Antibody (AAA19216, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IRF3, Polyclonal Antibody (Cat# AAA19216)
Full Name
Anti-IRF3 Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 13. Flow Cytometry analysis of A431 cells using anti-LSM8 antibody (AAA19337).Overlay histogram showing A431 cells stained with AAA19337 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LSM8 Antibody (AAA19337, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
LSM8, Polyclonal Antibody (Cat# AAA19337)
Full Name
Anti-LSM8 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of SiHa cells using anti-Aconitase 1/ACO1 antibody (AAA19267).Overlay histogram showing SiHa cells stained with AAA19267 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aconitase 1/ACO1 Antibody (AAA19267,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Aconitase 1/ACO1, Polyclonal Antibody (Cat# AAA19267)
Full Name
Anti-Aconitase 1/ACO1 Antibody
Gene Names
ACO1; IRP1; ACONS; IREB1; IREBP; IREBP1
Reactivity
Human, Mouse, Monkey, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of lysates from 293T, Hela cell line (from left to right), using GLS Antibody (C-term). AAA28657 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
GLS, Polyclonal Antibody (Cat# AAA28657)
Full Name
GLS Antibody (C-term)
Gene Names
GLS; GAC; GAM; KGA; GLS1; AAD20
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IP (Immunoprecipitation)
(NES was immunoprecipitated using: Lane A:0.5 mg U251MG Whole Cell Lysate 4 uL anti-NES mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NES mouse monoclonal antibody,at 1:100 dilution Secondary antibody: Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 177 kDa Observed band size :177 kDa)
Nestin, Monoclonal Antibody (Cat# AAA27730)
Full Name
Anti-Nestin Antibody, Mouse Monoclonal
Gene Names
NES; Nbla00170
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
Protein A
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of PC-3 cells using anti-MitoNEET/CISD1 antibody (AAA19293).Overlay histogram showing PC-3 cells stained with AAA19293 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MitoNEET/CISD1 Antibody (AAA19293,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MitoNEET/CISD1, Polyclonal Antibody (Cat# AAA19293)
Full Name
Anti-MitoNEET/CISD1 Antibody
Gene Names
CISD1; ZCD1; MDS029; C10orf70; mitoNEET
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 6. IF analysis of GRSF1 using anti- GRSF1 antibody (AAA19315).GRSF1 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- GRSF1 Antibody (AAA19315) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
GRSF1, Polyclonal Antibody (Cat# AAA19315)
Full Name
Anti-GRSF1 Antibody
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of U251 cells using anti-VPRBP/DCAF1 antibody (AAA19343).Overlay histogram showing U251 cells stained with AAA19343 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VPRBP/DCAF1 Antibody (AAA19343, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
VPRBP/DCAF1, Polyclonal Antibody (Cat# AAA19343)
Full Name
Anti-VPRBP/DCAF1 Antibody
Gene Names
DCAF1; RIP; VPRBP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of A431 cells using anti-TIMM8A/DDP antibody (AAA19317).Overlay histogram showing A431 cells stained with AAA19317 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIMM8A/DDP Antibody (AAA19317, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TIMM8A/DDP, Polyclonal Antibody (Cat# AAA19317)
Full Name
Anti-TIMM8A/DDP Antibody
Gene Names
TIMM8A; DDP; MTS; DDP1; DFN1; TIM8
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of K562 cells using anti-HP1 alpha/CBX5 antibody (AAA19266).Overlay histogram showing K562 cells stained with AAA19266 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HP1 alpha/CBX5 Antibody (AAA19266,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HP1 alpha/CBX5, Polyclonal Antibody (Cat# AAA19266)
Full Name
Anti-HP1 alpha/CBX5 Antibody
Gene Names
CBX5; HP1; HP1A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Flow cytometry analysis of GOT1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
GOT1, Monoclonal Antibody (Cat# AAA11733)
Full Name
GOT1 antibody
Gene Names
GOT1; AST1; cCAT; GIG18; cAspAT; ASTQTL1
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Pricing