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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
370 results for " F" - showing 350-370
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11850)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
Immunohistochemistry, Western Blot
Pricing
Reactivity Chart (RC)
TagRFP, Polyclonal Antibody (Cat# AAA13884)
Full Name
anti-TagRFP
Reactivity
Transfected cells proteins
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Immunoelectron Microscopy
Purity
This antibody is epitope-affinity purified from goat antiserum
Pricing
Application Data
(ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Fab Anti-Human IgG-HRP (AAA14906))
Goat F(ab) Anti-Human IgG (gamma chain specific), Secondary Antibody (Cat# AAA14906)
Full Name
Goat Fab Anti-Human IgG-HRP
Reactivity
Human
Applications
ELISA
Pricing
Application Data
(Anti-TurboRFP Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
TurboRFP, Polyclonal Antibody (Cat# AAA13881)
Full Name
anti-TurboRFP
Reactivity
Transfected cells proteins
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Immunoelectron Microscopy
Purity
This antibody is epitope-affinity purified from goat antiserum.
Pricing
IEM (Immunogold Electron Microscopy)
(Immunogold labeling of RPE, in vivo injected with mCherry expressing vector)
mCherry, Polyclonal Antibody (Cat# AAA13867)
Full Name
mCherry Polyclonal Antibody
Reactivity
Transfected cells proteins
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, Immunohistochemistry, Immunoelectron Microscopy
Pricing
IHC (Immunohistochemistry)
(Anti- TGM2 Picoband antibody, AAA11646, IHC(F)IHC(F): Human Placenta Tissue)
TGM2, Polyclonal Antibody (Cat# AAA11646)
Full Name
Anti-TGM2 Antibody
Gene Names
TGM2; TGC; TG(C)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H3, Polyclonal Antibody (Cat# AAA31353)
Full Name
Acetyl-Histone H3 (Lys122) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%)
Predicted Reactivity: Bovine (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistchemistry)
(Anti- SFTP A1/2 Picoband antibody, AAA11644, IHC(F)IHC(F): Mouse Lung Tissue)
SFTPA1/2, Polyclonal Antibody (Cat# AAA11644)
Full Name
Anti-SFTPA1/2 Antibody
Gene Names
SFTPA2; PSAP; PSPA; SP-A; SPA2; PSP-A; SFTP1; SPAII; COLEC5; SFTPA2B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
IA (Immuno Assay)
(used as capture antibody. Different concentrations were tested (0.5-2 ug/ml).)
Calprotectin, Monoclonal Antibody (Cat# AAA14609)
Full Name
Calprotectin, Human, mAb 27E10, Biotin
Reactivity
Mouse: No; Rhesus Monkey: Yes (subpopulations of macrophages)
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoassay, Immunoprecipitation, Western Blot
Purity
Protein G
Pricing
WB (Western Blot)
(Western Blot Analysis of Human Kidney lysate using Calnexin Mouse Monoclonal Antibody (CANX/1541).)
Calnexin, Monoclonal Antibody (Cat# AAA23894)
Full Name
Calnexin (Endoplasmic Reticulum Marker)
Gene Names
CANX; CNX; P90; IP90
Reactivity
Human.
Does not react with rat. Others not known.
Does not react with rat. Others not known.
Applications
Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(All lanes : Anti-hDCAMKL1-V705 at 1:8000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lane 3: Rat brain lysate Lane 4: TT whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
DCAMKL1, Polyclonal Antibody (Cat# AAA28779)
Full Name
DCAMKL1 Antibody (C-term)
Gene Names
DCLK1; CL1; DCLK; CLICK1; DCDC3A; DCAMKL1
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-Band 3 Antibody overnight at 4 degree C. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Band 3, Polyclonal Antibody (Cat# AAA11648)
Full Name
Anti-Band 3 Antibody
Gene Names
SLC4A1; DI; FR; SW; WD; WR; AE1; CHC; SAO; WD1; BND3; EPB3; SPH4; CD233; EMPB3; RTA1A
Reactivity
Human, Mouse , Rat.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
Rabbit anti Monkey IgG Fab, Polyclonal Secondary Antibody (Cat# AAA14570)
Full Name
Rabbit anti Monkey IgG Fab
Reactivity
Monkey
Applications
Immunofluorescence, Dot Blot, Immunoblot
Purity
Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Pricing
Application Data
(Human peripheral blood lymphocytes were stained with Goat F(ab’)2 Anti-Human IgD-AF488 and Mouse Anti-Human CD19-PE.)
Goat F(ab')2 Anti-Human IgD (delta chain specific), Polyclonal Secondary Antibody (Cat# AAA14902)
Full Name
Goat F(ab')2 Anti-Human IgD-UNLB
Reactivity
Human
Applications
Flow Cytometry
Purity
Gel filtration chromatography of pepsin digested antibody.
Pricing
FCM (Flow Cytometry)
(Flow Cytometry analyis of SSTR2 in human cell line. Human breast cancer cell line was stained with AAA14651 (filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 secondary antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.)
Somatostatin Receptor Type 2, Monoclonal Antibody (Cat# AAA14651)
Full Name
Somatostatin Receptor Type 2 (SSTr2)
Gene Names
SSTR2; SSTR2
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Flow Cytometry
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Pricing
IHC (Immunohistchemistry)
(HC analysis of Aquaporin 4 using anti-Aquaporin 4 antibody (AAA11657). Aquaporin 4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Aquaporin 4 Antibody (AAA11657) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # please inquire) with DAB as the chromogen.)
Aquaporin 4, Polyclonal Antibody (Cat# AAA11657)
Full Name
Anti-Aquaporin 4 Antibody
Gene Names
AQP4; MIWC; WCH4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
Application Data
(Published customer image: Mouse anti Pig IgM antibody, clone K52 1C3 used for thetection of IgM expressing cels in pig jejenum by immunofluorescence.Image caption:Colonisation expands IgM and IgA-producing mucosal plasma cells. (A) IgA staining in jejunal mucosa from a GF pig and (B) a colonised pig at 21 days of age. Sections were stained with antibodies to IgA (red) and capillary endothelium (MIL11; in blue). In both (A) and (B), (i) shows the two-colour image; (ii) shows the original greyscale image for the red channel (IgA); (iii) shows the original greyscale image for the blue channel (endothelium). Scale bar represents 10 um. (C) IgM staining in jejunal mucosa from a GF pig and (D) a colonised pig at 21 days of age. Sections were stained with antibodies to IgM (red). Scale bar represents 10 um. (E) Area of IgM and (F) IgA staining in jejunal sections from piglets at birth (open diamonds) and GF piglets (open squares) and colonised piglets (black squares) at 5 and 21days of age (see Table 1 for numbers of piglets in each experiment). * p)
IgM, Monoclonal Antibody (Cat# AAA12250)
Full Name
MOUSE ANTI PIG IgM
Reactivity
Pig
Applications
Flow Cytometry, Immunohistochemistry
Pricing
ApolipoproteinB-100, Antibody (Cat# AAA13602)
Full Name
FITC-conjugated Goat Anti-Human ApolipoproteinB-100
Gene Names
APOB; FLDB; LDLCQ4
Applications
Immunofluorescence
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of paraformaldehyde-fixed Ramos cells using CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.)
CD22/BL-CAM, Monoclonal Antibody (Cat# AAA23895)
Full Name
CD22/BL-CAM (B-Cell Marker)
Gene Names
CD22; SIGLEC2; SIGLEC-2
Reactivity
Human. Others not known.
Applications
Western Blot, Immunohistochemistry
Pricing
HLA DQ, Monoclonal Antibody (Cat# AAA14564)
Full Name
Mouse anti Human HLA DQ
Reactivity
Human, Swine
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Pricing