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384 results for " Flow Cytometry Antibodies" - showing 300-350
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA28788 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28788, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was Rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
IDUA, Polyclonal Antibody (Cat# AAA28788)
Full Name
IDUA Antibody (Center)
Gene Names
IDUA; IDA; MPS1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 ug/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.)
TIGIT, Monoclonal Antibody (Cat# AAA11005)
Full Name
TIGIT Antibody [4A11]
Gene Names
TIGIT; VSIG9; VSTM3; WUCAM
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Protein A purified
Pricing
HLA Class 1 Antigen B17,B35,B44(X-Reactive), Monoclonal Antibody (Cat# AAA14659)
Full Name
HLA Class 1 Antigen B17,B35,B44(X-Reactive)
Reactivity
Human
Purity
Ascites
Pricing
FCM (Flow Cytometry)
(BDKRB1 Antibody (Center) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
BDK_1, Polyclonal Antibody (Cat# AAA28760)
Full Name
BDK_1 Antibody (Center)
Gene Names
BDKRB1; B1R; BKR1; B1BKR; BKB1R; BDKRB2; BRADYB1
Reactivity
Human
Applications
Western Blot, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data
(Staining of human peripheral blood granulocytes with CD18: Alexa Fluor 647)
CD18, Monoclonal Antibody (Cat# AAA11881)
Full Name
RAT ANTI HUMAN CD18:FITC
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
Flow Cytometry
Pricing
Application Data
(Staining of human peripheral blood granulocytes with CD18: Alexa Fluor 647)
CD18, Monoclonal Antibody (Cat# AAA11987)
Full Name
RAT ANTI HUMAN CD18
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
FCM (Flow Cytometry)
(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FOLH1 antibody, and then analyzed by flow cytometry.)
FOLH1 / PSMA, Monoclonal Antibody (Cat# AAA12376)
Full Name
Anti-FOLH1 / PSMA Antibody (clone 3H5) IHC-plus
Gene Names
FOLH1; PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
Reactivity
Mouse, Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Protein A/G purified
Pricing
FCM (Flow Cytometry)
(WB - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail FC - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail Overlay histogram showing HepG2 cells stained with AAA28757(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28757, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA28757)
Full Name
MCSF Receptor (CSF1R) Antibody (C-term)
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD40:RPE)
CD40, Monoclonal Antibody (Cat# AAA12090)
Full Name
MOUSE ANTI HUMAN CD40
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry
Pricing
Application Data
(Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:FITC)
CD61, Monoclonal Antibody (Cat# AAA11873)
Full Name
HAMSTER ANTI MOUSE CD61:FITC
Gene Names
Itgb3; CD61; GP3A; INGRB3
Applications
Flow Cytometry
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NANOG Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NANOG primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NANOG immunoreactivity is localized to Nucleus significantly.)
NANOG, Polyclonal Antibody (Cat# AAA28706)
Full Name
NANOG Antibody (N-term)
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD40:RPE)
CD40, Monoclonal Antibody (Cat# AAA11867)
Full Name
MOUSE ANTI HUMAN CD40:FITC
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
Flow Cytometry
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD40:RPE)
CD40, Monoclonal Antibody (Cat# AAA11936)
Full Name
MOUSE ANTI HUMAN CD40
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Reactivity
Dog
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12197)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)
STIP1, Monoclonal Antibody (Cat# AAA26408)
Full Name
STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (FITC)
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Pricing
WB (Western Blot)
(Anti-Desmoglein 2 antibody, AAA11514, Western blottingAll lanes: Anti Desmoglein 2 (AAA11514) at 0.5ug/mlLane 1: HT1080 Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: SW620 Whole Cell Lysate at 40ugLane 4: SCG Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 122KDObserved bind size: 122KD)
Desmoglein 2, Polyclonal Antibody (Cat# AAA11514)
Full Name
Polyclonal Anti-Desmoglein 2, DSG2
Gene Names
DSG2; HDGC; CDHF5; ARVC10; ARVD10; CMD1BB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
FCM (Flow Cytometry)
(Detection of Carbonic Anhydrase IX/CA9 in U‑87 MG Human Cell Line by Flow Cytometry using AAA14680.)
Carbonic Anhydrase IX, Monoclonal Antibody (Cat# AAA14680)
Full Name
Carbonic Anhydrase IX (CA9) (PE)
Gene Names
CA9; MN; CAIX
Applications
Flow Cytometry
Purity
Affinity Purified
Purified by Protein A affinity chromatography from hybridoma culture supernant.
Purified by Protein A affinity chromatography from hybridoma culture supernant.
Pricing
FCM (Flow Cytometry)
(PCSK9 Antibody (N-term) (Cat. #AAA28774) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell(top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
PCSK9, Polyclonal Antibody (Cat# AAA28774)
Full Name
PCSK9 Antibody (N-term)
Gene Names
PCSK9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD40:RPE)
CD40, Monoclonal Antibody (Cat# AAA12089)
Full Name
MOUSE ANTI HUMAN CD40:RPE
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12193)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
Flow Cytometry
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Pacific Blue)
CD4, Monoclonal Antibody (Cat# AAA12071)
Full Name
MOUSE ANTI HUMAN CD4:FITC
Gene Names
CD4; CD4mut
Reactivity
Human
Applications
Flow Cytometry
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD40:RPE)
CD40, Monoclonal Antibody (Cat# AAA12031)
Full Name
MOUSE ANTI HUMAN CD40:RPE
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12192)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)
STIP1, Monoclonal Antibody (Cat# AAA26275)
Full Name
STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (Biotin)
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12093)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA
Reactivity
Dog, Dolphin, Ferret, Fin Whale, Goat, Horse, Human, Mink, Rabbit, Pig, Sheep.
Based on sequence similarity, is expected to react with: Mustelid
N.B. Antibody reactivity and working conditions may vary between species.
Based on sequence similarity, is expected to react with: Mustelid
N.B. Antibody reactivity and working conditions may vary between species.
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12194)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12198)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
Flow Cytometry
Pricing
CD105, Monoclonal Antibody (Cat# AAA14292)
Full Name
CD105 antibody (biotin)
Reactivity
To be determined by end-user.
Applications
Flow Cytometry, Immunohistochemistry
Purity
>95% pure
CD105 antibody (biotin) was purified by Protein A chromatography.
CD105 antibody (biotin) was purified by Protein A chromatography.
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12195)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
Application Data
(Fig-5: Cell Surface staining of human ACE2 in the ACE2/HEK293 stable cell line. ATTO 488-conjugated anti-hACE2 antibody (clone AC18F; was used at 1ug/ 1x10^6 cells (Red). ATTO 488-conjugated mouse IgG1 was used as isotype control at 1ug/ 1x10^6 cells (Green).)
ACE2, Cell Line (Cat# AAA14888)
Full Name
ACE2/HEK293 Stable Cell Line
Gene Names
ACE2; ACEH
Applications
Functional Assay
Pricing
WB (Western Blot)
(HHLA2 Antibody (N-term) western blot analysis in MDA-MB453,CEM,HL-60,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HHLA2 antibody detected the HHLA2 protein (arrow).)
HHLA2, Polyclonal Antibody (Cat# AAA28722)
Full Name
HHLA2 Antibody (N-term)
Gene Names
HHLA2; B7H7; B7-H7
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12095)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:FITC
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12096)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:RPE
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 488)
CD81, Monoclonal Antibody (Cat# AAA11869)
Full Name
HAMSTER ANTI MOUSE CD81:FITC
Gene Names
Cd81; Tapa1; Tapa-1; Tspan28
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 488)
CD81, Monoclonal Antibody (Cat# AAA12033)
Full Name
HAMSTER ANTI MOUSE CD81:RPE
Gene Names
Cd81; Tapa1; Tapa-1; Tspan28
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12190)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12189)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:Low Endotoxin
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC AAA12191)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12191)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(PE conjugated polystyrene beads were stained with (filled histogram) or rabbit IgG, FITC isotype control (open histogram).)
R-PE, Antibody (Cat# AAA14282)
Full Name
Purified Rabbit Anti-R-PE, Polyclonal Antibody
Applications
Flow Cytometry, Immunohistochemistry
Pricing
FCM (Flow Cytometry)
(CB2 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
CB2, Polyclonal Antibody (Cat# AAA28672)
Full Name
CB2 Antibody (C-term)
Gene Names
CNR2; CB2; CX5; CB-2
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12188)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:Biotin
Applications
Flow Cytometry
Pricing
HLA Class 1 Antigen A23,A24, Monoclonal Antibody (Cat# AAA14677)
Full Name
HLA Class 1 Antigen A23,A24
Reactivity
Human
Applications
Cell Typing
Purity
Ascites
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12094)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:Biotin
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12196)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12199)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(PYY Antibody (C-term) (AAA28733) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
PYY, Polyclonal Antibody (Cat# AAA28733)
Full Name
PYY Antibody (C-term)
Gene Names
PYY; PYY1; PYY-I
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 2 ug/ml on h Spleen lysate.)
PD-L1, Monoclonal Antibody (Cat# AAA14873)
Full Name
Monoclonal Antibody to PD-L1 (Clone: ABM4E54)
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
Purified
Protein G Chromatography
Protein G Chromatography
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA28750 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28750, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events wasperformed.)
WNT5A, Polyclonal Antibody (Cat# AAA28750)
Full Name
WNT5A Antibody (Center)
Gene Names
WNT5A; hWNT5A
Reactivity
Human, Mouse, Rat
Predicted: Rabbit
Predicted: Rabbit
Applications
Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of SLC10A1 using anti- SLC10A1 antibody (AAA11653).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates (positive control), Lane 2: rat kidney tissue lysates, (negative control)After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1 antigen affinity purified polyclonal antibody (AAA11653) at 0.25ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50KD. The expected band size for SLC10A1 is at 38KD.)
SLC10A1, Polyclonal Antibody (Cat# AAA11653)
Full Name
Anti-SLC10A1 Antibody
Gene Names
Slc10a1; Ntcp
Reactivity
Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11930)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing