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Type
Reactivity
Gene Name
Isotype
Host
Application
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3602 results for " Antibodies" - showing 3300-3350
FCM (Flow Cytometry)
(CB2 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
CB2, Polyclonal Antibody (Cat# AAA28672)
Full Name
CB2 Antibody (C-term)
Gene Names
CNR2; CB2; CX5; CB-2
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(Anti-NR6A1 / GCNF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
NR6A1 / GCNF, Polyclonal Antibody (Cat# AAA12361)
Full Name
Rabbit Polyclonal to Human NR6A1 / GCNF
Gene Names
NR6A1; RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications
Immunohistochemistry
Purity
Immunoaffinity Purified
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12188)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:Biotin
Applications
Flow Cytometry
Pricing
Thrombocyte, Polyclonal Antibody (Cat# AAA14283)
Full Name
Anti-Mouse Thrombocyte, Antiserum, (Polyclonal) (rabbit serum)
Reactivity
Mouse
Applications
Agglutination Assay, Invivo Depletion
Pricing
Structure
Digoxin, Monoclonal Antibody (Cat# AAA11716)
Full Name
Digoxin Monoclonal Antibody
Applications
ELISA
Purity
Protein G Purified
Pricing
WB (Western Blot)
(lane C, 293 cell extract controlLane 1, 293 cell expressing HA2 (H5N1)Lane 2, 293 cell expressing HA (H5N1))
HA2 (A/Vietnam/1203/2004)(H5N1), Polyclonal Antibody (Cat# AAA13718)
Full Name
Anti-HA2 (A/Vietnam/1203/2004)(H5N1), rabbit IgG
Applications
Western Blot
Purity
Immunoaffinity chromatography
Pricing
Application Data
H5 (A/Hong Kong/483/97)(H5N1), Polyclonal Antibody (Cat# AAA13731)
Full Name
Anti-H5 (A/Hong Kong/483/97)(H5N1), rabbit IgG
Reactivity
Has not been tested.
Applications
Western Blot
Purity
Immunoaffinity chromatography
Pricing
Myeloperoxidase, Monoclonal Antibody (Cat# AAA13376)
Full Name
MAb to Myeloperoxidase
Gene Names
MPO; MPO
Applications
ELISA
Purity
>90% pure (SDS-PAGE). Protein A chromatography
Pricing
IHC (Immunohistochemistry)
(Figure-8: Immunohistochemical analysis of SLC31A1 in human Stomach tissue using Anti-SLC31A1 antibody (Clone: ABM5D89) at 5 ug/ml.)
SLC31A1, Monoclonal Antibody (Cat# AAA14884)
Full Name
Monoclonal antibody to SLC31A1 (Clone: ABM5D89)
Gene Names
SLC31A1; CTR1; COPT1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Protein G Chromatography
Pricing
IF (Immunofluorescence)
(Leu-Enkephalin is an endogenous opioid peptide that interacts with opioid receptors and produces analgesic effects.Images: Immunohistochemical detection of Leu-Enkephalin in rat ventral periaqueductal grey matter (PAG). Brain was fixed with 4% formaldehyde and cut into 10 ?m thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to Leu-Enkephalin at 10 ?g/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red (pseudo-coloured yellow) conjugated secondary antibodies at 1:200. Tissue was counterstained with DAPI (blue color) to visualize cell nuclei.)
Leu-Enkephalin, Antibody (Cat# AAA14441)
Full Name
Leu-Enkephalin, Affinity Purified Antibody, Rabbit
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry
Pricing
HLA Class 1 Antigen A23,A24, Monoclonal Antibody (Cat# AAA14677)
Full Name
HLA Class 1 Antigen A23,A24
Reactivity
Human
Applications
Cell Typing
Purity
Ascites
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PMS1 antibody (AAA28270) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
PMS1, Polyclonal Antibody (Cat# AAA28270)
Full Name
PMS1 Polyclonal Antibody
Gene Names
PMS1; MLH2; PMSL1; hPMS1; HNPCC3
Applications
Western Blot
Purity
Affinity Purification
Pricing
Thyroid Stimulating Hormone (TSH), Monoclonal Antibody (Cat# AAA13380)
Full Name
MAb to TSH
Applications
ELISA
Purity
>=90% pure (SDS-PAGE). Protein A chromatography. Product is 0.2um filtered.
Pricing
WB (Western Blot)
(RPS6KB1 monoclonal antibody (M03), clone 2C2. Western Blot analysis of RPS6KB1 expression in PC-12.)
RPS6KB1, Monoclonal Antibody (Cat# AAA26369)
Full Name
RPS6KB1 (Ribosomal Protein S6 Kinase, 70kD, Polypeptide 1, PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha) (FITC)
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Applications
Immunofluorescence, Western Blot
Purity
Purified
Pricing
FUSIP1, Monoclonal Antibody (Cat# AAA24513)
Full Name
FUSIP1 (SRSF10, FUSIP1, FUSIP2, SFRS13A, TASR, Serine/Arginine-rich Splicing Factor 10, 40kD SR-repressor Protein, FUS-interacting Serine-arginine-rich Protein 1, Splicing Factor SRp38, Splicing Factor, Arginine/Serine-rich 13A, TLS-associated Protein wit
Gene Names
SRSF10; NSSR; TASR; SRp38; TASR1; TASR2; FUSIP1; FUSIP2; SFRS13; SRrp40; SFRS13A; PPP1R149
Reactivity
Human
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of BMP2 expression in HepG2 (A); HeLa (B) whole cell lysates.)
BMP2, Polyclonal Antibody (Cat# AAA12380)
Full Name
Anti-BMP2 Antibody (C-Terminus) IHC-plus
Gene Names
BMP2; BDA2; BMP2A
Reactivity
Chicken, Zebrafish, Pig, Rabbit, Mouse, Sheep, Bovine, Rat, Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunoaffinity purified
Pricing
IHC (Immunohistochemistry)
(Anti-SLC7A5 antibody IHC of human placenta, terminal villi. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
SLC7A5 / CD98, Polyclonal Antibody (Cat# AAA12328)
Full Name
SLC7A5 / CD98 Light Chain
Gene Names
SLC7A5; E16; CD98; LAT1; 4F2LC; MPE16; hLAT1; D16S469E
Reactivity
Human, Monkey, Dog.
Predicted: Bat, Rabbit
Predicted: Bat, Rabbit
Applications
Immunohistochemistry
Purity
Immunoaffinity Purified
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
eNOS, Polyclonal Antibody (Cat# AAA31394)
Full Name
Phospho-eNOS (Ser1179) Antibody
Gene Names
NOS3; eNOS; ECNOS
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (100%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Double-stranded RNA (dsRNA), Monoclonal ELISA Kit (Cat# AAA14576)
Full Name
Double-stranded RNA (dsRNA) ELISA kit (J2 based)
Reactivity
General
Applications
ELISA
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12094)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:Biotin
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12196)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12199)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
Flow Cytometry
Pricing
Reovirus, Polyclonal Antibody (Cat# AAA14712)
Full Name
Reovirus
Applications
ELISA
Purity
Sterile filtered serum
Pricing
SCCA1, Recombinant Protein (Cat# AAA13527)
Full Name
SCCA1 Protein (Active)
Applications
ELISA, SDS-PAGE
Purity
>95% by SDS-PAGE
Pricing
WB (Western Blot)
(Western Blot Data: C: 293 cell extract controlE: 293 cell expressing gp120 (SIV/mac239))
gp120/160 (SIV/mac239), Polyclonal Antibody (Cat# AAA13713)
Full Name
Anti-gp120/160 (SIV-1/mac239) Rabbit Polyclonal Antibody
Applications
Western Blot
Purity
Immunoaffinity chromatography
Pricing
Mycobacterium tuberculosis CFP10, Monoclonal Antibody (Cat# AAA14662)
Full Name
Mycobacterium tuberculosis CFP10
Applications
ELISA
Purity
Purified by Protein G affinity chromatography from ascites (>95% pure)
Pricing
Human Papilloma Virus Type 18 (HPV) E7 Protein, Monoclonal Antibody (Cat# AAA13368)
Full Name
MAb to HPV 18 (E7)
Applications
Western Blot
Purity
> 95% pure. Protein G Sepharose chromatography. Purity is tested by electrophoresis.
Pricing
Chlamydia trachomatis LPS, Monoclonal Antibody (Cat# AAA13395)
Full Name
MAb to C. trachomatis LPS
Applications
Lateral Flow
Purity
Protein G chromatography
Pricing
Application Data
(Detection of HA-tagged fusion protein in 200,100, and 50 ng of E. coli cell lysate with AAA11721 diluted 1:25,000)
HA HRP, Polyclonal Antibody (Cat# AAA11721)
Full Name
HA Affinity Purified Antibody, HRP Conjugated
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Antibody was purified by peptide affinity chromatography and conjugated to horseradish peroxidase (HRP).
Pricing
Rhinovirus outer capsid Protein 3, Monoclonal Antibody (Cat# AAA13450)
Full Name
Rhinovirus outer capsid Protein 3 (VP3)
Applications
Immunofluorescence
Purity
> 90% pure. Protein A Chromatography.
Pricing
WB (Western Blot)
(Western Blot of Rat brain using AAA14727 (1ug/mL). The signal was developed with IRDyeTM800 conjugated giat anti-rabbit IgG)
Tau, Polyclonal Antibody (Cat# AAA14727)
Full Name
Tau, phosphorylated (Thr217) (Tau Protein, Microtubule-associated Protein, MAP)
Gene Names
tau; BcDNA:RE16764; CG12881; CG31057; CG5606; DmelCG31057; dtau
Reactivity
Human
Applications
Western Blot
Purity
Purified by Affinity chromatography.
Pricing
IHC (Immunohistochemistry)
(Rat Skeletal Muscle- MOTS-c Primary Antibody: AAA14413; 1:50 dilution in IHC Blocking Buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification on Leica DM4000. FFPE section.)
MOTS-c, Polyclonal Antibody (Cat# AAA14413)
Full Name
Rabbit Polyclonal Anti-MOTS-c antibody
Applications
Immunoprecipitation, Western Blot, Dot Blot
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cells, using CYCS antibody.)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26880)
Full Name
AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (MaxLight 650)
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Mouse, Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cell using TYMP antibody. Blue: DAPI for nuclear staining.)
TYMP, Polyclonal Antibody (Cat# AAA29697)
Full Name
TYMP Antibody
Gene Names
TYMP; TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
Mycoplasma hominis, p120, Monoclonal Antibody (Cat# AAA13388)
Full Name
MAb to Mycoplasma hominis
Applications
Immunofluorescence
Purity
>90% pure. Protein A chromatography
Pricing
WB (Western Blot)
(WB: A431 cell lysate was probed with anti-Catenin, beta, active (ABC). Arrow indicates beta-Catenin (92kD))
Catenin, beta, active, Monoclonal Antibody (Cat# AAA14699)
Full Name
Catenin, beta, active (ABC) (BSA, Azide & Glycerol Free)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Purified by Protein G affinity chromatography.
Pricing
Rotavirus NCDV (all antigens), Polyclonal Antibody (Cat# AAA13407)
Full Name
Goat anti Rotavirus
Applications
Immunofluorescence
Purity
> 95% pure. Sodium sulfate precipitation and ion exchange chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 2 ug/ml on h Spleen lysate.)
PD-L1, Monoclonal Antibody (Cat# AAA14873)
Full Name
Monoclonal Antibody to PD-L1 (Clone: ABM4E54)
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
Purified
Protein G Chromatography
Protein G Chromatography
Pricing
FCM (Flow Cytometry)
(PYY Antibody (C-term) (AAA28733) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
PYY, Polyclonal Antibody (Cat# AAA28733)
Full Name
PYY Antibody (C-term)
Gene Names
PYY; PYY1; PYY-I
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IS (Immunostaining)
(Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (AAA14229, red, 1:500) and anti-MAP2 antibody (, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.)
Nestin, Monoclonal Antibody (Cat# AAA14229)
Full Name
Anti-Nestin
Gene Names
NES; Nbla00170
Applications
Western Blot, Immunofluorescence
Purity
Protein G purified culture supernatant
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27671)
Full Name
VPS35 Antibody, Clone 7E4: PerCP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27680)
Full Name
VPS35 Antibody, Clone 5A9: PerCP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IF (Immunofluorescence)
(Figure 2. Indirect immunofluorescence staining of human kidney tissue section with (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.)
vimentin, Monoclonal Recombinant Antibody (Cat# AAA14575)
Full Name
Recombinant rabbit anti vimentin
Reactivity
Human, mouse
Applications
Immunocytochemistry, Immunohistochemistry, Immunoblot
Purity
The purity of the antibody is >98% as determined by SDS-Polyacrylamide gel electrophoresis.
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA28750 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28750, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events wasperformed.)
WNT5A, Polyclonal Antibody (Cat# AAA28750)
Full Name
WNT5A Antibody (Center)
Gene Names
WNT5A; hWNT5A
Reactivity
Human, Mouse, Rat
Predicted: Rabbit
Predicted: Rabbit
Applications
Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of SLC10A1 using anti- SLC10A1 antibody (AAA11653).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates (positive control), Lane 2: rat kidney tissue lysates, (negative control)After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1 antigen affinity purified polyclonal antibody (AAA11653) at 0.25ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50KD. The expected band size for SLC10A1 is at 38KD.)
SLC10A1, Polyclonal Antibody (Cat# AAA11653)
Full Name
Anti-SLC10A1 Antibody
Gene Names
Slc10a1; Ntcp
Reactivity
Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11864)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
Immunofluorescence
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11930)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
IgG, Monoclonal Antibody (Cat# AAA13386)
Full Name
MAb to Human IgG
Applications
Lateral Flow
Purity
97.62 % (Ionic Exchange Chromatography)
Pricing
Application Data
HA1 (A/chicken/MD/MINHMA/2004)(H7N2), Polyclonal Antibody (Cat# AAA13716)
Full Name
Anti-HA1 (A/chicken/MD/MINHMA/2004)(H7N2)
Applications
Western Blot
Purity
Immunoaffinity chromatography
Pricing
C-Reactive Protein, Native Protein (Cat# AAA10843)
Full Name
Human C-Reactive Protein
Gene Names
CRP; PTX1
Purity
Greater than 96.0%.
Pricing