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Application
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3248 results for " Other" - showing 3000-3050
SDS-PAGE
Zika Virus Envelope, Recombinant Protein (Cat# AAA13452)
Full Name
Zika Virus Envelope Recombinant
Applications
Chemiluminescence Immunoassay, Lateral Flow
Purity
> 80% Pure (SDS-PAGE). Affinity Chromatography
Pricing
Knockout Validation
(Knockout Validation: Lane 1: Wild-type PC3 cell lysate; Lane 2: ANTXR2 knockout PC3 cell lysate; Predicted MW: 54,53,43,35kd Observed MW: 58kd Primary Ab: 1ug/ml Rabbit Anti-Human ANTXR2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Anthrax Toxin Receptor 2, Polyclonal Antibody (Cat# AAA21007)
Full Name
Anthrax Toxin Receptor 2 (ANTXR2) Polyclonal Antibody
Gene Names
ANTXR2; HFS; ISH; JHF; CMG2; CMG-2
Reactivity
Human, Rat, Pig
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
Affinity Chromatography
Pricing
IHC (Immunohistochemistry)
(Figure 3. IHC analysis of SFTPA1 using anti- SFTPA1 antibody (AAA11521).SFTPA1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- SFTPA1 Antibody (AAA11521) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Surfactant Protein A, Polyclonal Antibody (Cat# AAA11521)
Full Name
Polyclonal Anti-SFTPA1 Antibody
Gene Names
Sftpa1; SP-A; Sftp1; Sftp-1
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
HRI, Polyclonal Antibody (Cat# AAA28727)
Full Name
HRI Antibody (N-term)
Gene Names
EIF2AK1; HCR; HRI
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12211)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
parvovirus B19 IgM, B19IgM, ELISA Kit (Cat# AAA29410)
Full Name
Human parvovirus B19 IgM, B19IgM ELISA Kit
Reactivity
Human
Pricing
H1 (A/California/04/2009)(H1N1), Polyclonal Antibody (Cat# AAA13734)
Full Name
Anti-H1 (aa 200-260)(A/California/04/2009)(H1N1)
Applications
Western Blot
Purity
Purity: >95%
Purification: Immunoaffinity chromatography
Purification: Immunoaffinity chromatography
Pricing
M2 (A/Brisbane/10/2007)(H3N2), Polyclonal Antibody (Cat# AAA13736)
Full Name
Anti-M2 (A/Brisbane/10/2007)(H3N2), antigen (SLLTEVETP IRNEWGCRCN DSSD)
Applications
Western Blot
Purity
Immunoaffinity chromatography
Pricing
Flavivirus, Group, Monoclonal Antibody (Cat# AAA14673)
Full Name
Flavivirus, Group
Applications
Immunofluorescence
Purity
Affinity Purified
Purified by Protein A affinity chromatography from ascites.
Purified by Protein A affinity chromatography from ascites.
Pricing
Sm/RNP Antigen, Native Protein (Cat# AAA13421)
Full Name
Sm/RNP Antigen
Applications
ELISA
Purity
>90% pure (SDS-PAGE Electrophoresis)
Pricing
Bacillus cereus/Bacillus subtilis, Spores, Polyclonal Antibody (Cat# AAA14710)
Full Name
Rabbit anti-Bacillus cereus/Bacillus subtilis, Spores
Applications
Immunofluorescence, Fluorescence Microscopy
Purity
Purified IgG fraction to a purity of > 95%.
Pricing
Pan pLDH, Monoclonal Antibody (Cat# AAA13403)
Full Name
MAb to Pan pLDH
Applications
Lateral Flow
Purity
>90% pure (SDS-PAGE). Protein G Chromatography
Pricing
Growth Hormone, Recombinant, Recombinant Protein (Cat# AAA13447)
Full Name
hGH, Recombinant
Gene Names
GH1; GH; GHN; GH-N; hGH-N; IGHD1B
Purity
>98% pure (RP-HPLC and SDS-PAGE). Purified by chromatographic techniques. Product is sterile filtered.
Pricing
Defensin, beta-2 (a.a. 4-41), Monoclonal Antibody (Cat# AAA13383)
Full Name
MAb to beta-Defensin-2 (a.a. 4-41)
Gene Names
Defb2; BD-2; MGC129140; MGC129141
Applications
ELISA
Purity
Purified, Lyophilized
Purification: Protein G Chromatography
Purification: Protein G Chromatography
Pricing
Creatine Kinase MM (CK-MM), Antibody (Cat# AAA13411)
Full Name
Goat anti CK-MM
Gene Names
CKM; CKMM; M-CK
Applications
Lateral Flow
Purity
Immunoaffinity column chromatography
Pricing
Rhinovirus outer capsid protein 3, Monoclonal Antibody (Cat# AAA13449)
Full Name
Rhinovirus outer capsid protein 3 (VP3)
Applications
Immunofluorescence
Purity
> 90% pure. Protein A Chromatography
Pricing
CA 125, Monoclonal Antibody (Cat# AAA14303)
Full Name
CA 125 antibody
Applications
Immunoblot, Immunoassay
Pricing
COVID 19 Spike S1 Protein Humanized Coronavirus, Monoclonal Antibody (Cat# AAA13537)
Full Name
Anti-SARS-CoV-2 S1 / COVID-19, Humanized antibody (Detection Ab)
Reactivity
Viral
Applications
Lateral Flow
Purity
Purity: >95% by HPLC & SDS-PAGE
Purification: Protein A purified
Purification: Protein A purified
Pricing
WB (Western Blot)
(Raji cells were subjected to SDS PAGE followed by western blot with AAA27490 (IL22RA2 Antibody) at dilution of 1:1000)
IL22RA2, Monoclonal Antibody (Cat# AAA27490)
Full Name
IL22RA2 Mouse Monoclonal
Gene Names
IL22RA2; CRF2X; CRF2-10; CRF2-S1; IL-22BP; IL-22RA2; ZCYTOR16; IL-22R-alpha-2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purification: Protein A+G purification
Purity: > = 95% as determined by SDS-PAGE
Purity: > = 95% as determined by SDS-PAGE
Pricing
COVID 19 Spike RBD IgG Humanized Coronavirus, Monoclonal Antibody (Cat# AAA13540)
Full Name
Anti-SARS-CoV-2 Spike RBD IgG, Humanized antibody (Positive Control)
Reactivity
Human
Applications
Lateral Flow
Purity
Purity: >95% by HPLC & SDS-PAGE
Purification: Protein A purified
Purification: Protein A purified
Pricing
HLA Class 1 Antigen A30,A31, Monoclonal Antibody (Cat# AAA14656)
Full Name
Mouse Anti-Human HLA Class 1 Antigen A30,A31 (R0.87)
Gene Names
HLA-A; HLAA
Reactivity
Human
Applications
Cell Typing
Purity
Ascites
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin embedded rat thymus sections using AAA14692 at a 1:250 dilution and detected by Alexa-488, which appears fluorescent green. 8-oxo-dG is present in the cytoplasm and nucleus of most but not all cells. Sections were counter-stained with 7-Aminoactinomycin D (7AAD), which labels nuclear DNA. Images were captured at 40X magnification.)
8-Hydroxy-2'-deoxyguanosine, Monoclonal Antibody (Cat# AAA14692)
Full Name
Mouse anti-8-Hydroxy-2'-deoxyguanosine (8-oxo-dG)
Applications
Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Purity
Purified from ascites.
Pricing
WB (Western Blot)
(Anti-Caspase-1(P20) antibody, AAA11584, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Spleen Tissue LysateLane 3: Mouse Brain Tissue LysateLane 4: Mouse Spleen Tissue LysateLane 5: Mouse Testis Tissue Lysate)
Caspase-1(P20), Polyclonal Antibody (Cat# AAA11584)
Full Name
Polyclonal Anti-CASP1 (p20) Antibody
Gene Names
Casp1; ICE; Il1bc
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Pricing
CRP, Polyclonal Antibody (Cat# AAA14448)
Full Name
Goat anti-Dog CRP - Antiserum
Gene Names
CRP; PTX1; MGC88244; MGC149895
Reactivity
Dog
Applications
ELISA
Pricing
WB (Western Blot)
(Western Blot analysis for galactocerebroside in PC12 lysate. PC12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with AAA14685 at 1:500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein Galactocerebroside (~75kD).)
Galactocerebroside, Monoclonal Antibody (Cat# AAA14685)
Full Name
Mouse anti-Bovine Galactocerebroside
Applications
Immunohistochemistry, Immunocytochemistry
Purity
Purified by Protein A affinity chromatography.
Pricing
FCM (Flow Cytometry)
(Flow Cytometry detection of FOLR1 in MCF-7 human cell line using AAA14691. MCF-7 human breast cancer cell line was stained with AAA14691 (filled histogram) or isotype control antibody (open histogram), followed byPhycoerythrin-conjugated Anti-MouseIgG Secondary Antibody.)
FOLR1, Monoclonal Antibody (Cat# AAA14691)
Full Name
FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)
Gene Names
FOLR1; FBP; FOLR
Reactivity
Human
Applications
Flow Cytometry, Western Blot, Immunocytochemistry
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Pricing
WB (Western Blot)
(All lanes : Anti-ERVK-7 Antibody (N-Term) at 1:1000-1:2000 dilutionLane 1: Raji whole cell lysateLane 2: human lung lysateLane 3: human testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 67 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
ERVK-7, Polyclonal Antibody (Cat# AAA28797)
Full Name
ERVK-7 Antibody (N-Term)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Goat anti Human IgG IgA IgM (Fc specific), Polyclonal Secondary Antibody (Cat# AAA14569)
Full Name
Goat anti Human IgG IgA IgM (Fc specific), conjugated with FITC
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Purity
The IgG (7S) fraction is isolated and purified from hyperimmune antisera with strong precipitating activity and contains the bulk of the antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodi
Pricing
WB (Western Blot)
(Figure 6. Western blot analysis of Adiponectin using anti-Adiponectin antibody (AAA11602).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Testis Tissue Lysate,Lane 2: Rat Spleen Tissue Lysate,Lane 3: Rat Skeletal Muscle Tissue Lysate,Lane 4: Rat Cardiac Muscle Tissue Lysate,Lane 5: Rat Liver Tissue Lysate,After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Adiponectin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Adiponectin at approximately 26KD. The expected band size for Adiponectin is at 26KD.)
Adiponectin, Polyclonal Antibody (Cat# AAA11602)
Full Name
Anti-Adiponectin antibody
Gene Names
Adipoq; Acdc; Acrp30
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
Insulin-like growth factors 1, ELISA Kit (Cat# AAA13215)
Full Name
Fish Insulin-like growth factors 1 (IGF-1) ELISA Kit
Reactivity
Fish
Pricing
Application Data
SAPOSIN (PSAP), Antibody (Cat# AAA14090)
Full Name
Rabbit anti Saposin antibody
Reactivity
Human
Applications
Western Blot
Purity
The Rabbit IgG is purified by specific Epitope Affinity Purification.
Pricing
WB (Western Blot)
(Western Blot Analysis using AAA14655: Lane 1: MW Marker; Lane 2: DnaK active recombinant protein, Lane 3: E. coli cell lysate.)
DnaK, Monoclonal Antibody (Cat# AAA14655)
Full Name
DnaK
Reactivity
E Coli
Applications
Western Blot
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
pf LDH, Monoclonal Antibody (Cat# AAA13405)
Full Name
MAb to pf LDH
Applications
Lateral Flow
Purity
< 90% pure (SDS-PAGE). Protein G Chromatography
Pricing
Goat Anti-Human IgG (γ chain specific), Secondary Antibody (Cat# AAA14903)
Full Name
Goat Anti-Human IgG-AP
Reactivity
Human
Applications
Flow Cytometry
Purity
Affinity chromatography on human IgG covalently linked to agarose
Pricing
Application Data
(Human peripheral blood lymphocytes were stained with Goat F(ab')2 antihuman IgM-PE and mouse anti-human CD19-FITC .)
Goat F(ab')2 Anti-Human IgM, Secondary Antibody (Cat# AAA14912)
Full Name
Goat F(ab')2 Anti-Human IgM-HRP
Reactivity
Human
Applications
Flow Cytometry
Pricing
Retinoic Acid Receptor Responder 2, Recombinant Protein (Cat# AAA10868)
Full Name
Recombinant Human Retinoic Acid Receptor Responder 2
Gene Names
RARRES2; TIG2; HP10433
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Human Papilloma Virus Type 18, Antigen (Cat# AAA13435)
Full Name
HPV18 L1 Protein, Recombinant
Applications
Immunoblot
Purity
>90% pure (SDS-PAGE). Purification from broken yeast cells by the following scheme: clarification,ammonium sulfate precipitation, ultrafiltration, cation exchange chromatography, zonal centrifugation, size exclusion chromatography and filtration.
Pricing
Hepatitis B Surface Antigen (HBsAg) (ad/ay), Monoclonal Antibody (Cat# AAA13387)
Full Name
MAb to HBsAg (ad/ay)
Applications
Lateral Flow
Purity
Ion exchange chromatography
Pricing
CA 125, Monoclonal Antibody (Cat# AAA14299)
Full Name
CA 125 antibody
Reactivity
CA 125 100%
Epitope specificity: "M-11 like" Group B
Epitope specificity: "M-11 like" Group B
Applications
ELISA
Pricing
Gram Positive Bacteria, Monoclonal Antibody (Cat# AAA13365)
Full Name
MAb to Gram Positive Bacteria
Applications
Immunofluorescence
Purity
>90% pure. Protein A chromatography
Pricing
IHC (Immunohistochemistry)
(Mast Cell Tryptase was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- Mast Cell Tryptase Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
Mast Cell Tryptase, Polyclonal Antibody (Cat# AAA11674)
Full Name
Anti-Mast Cell Tryptase Antibody
Gene Names
TPSAB1; TPS1; TPS2; TPSB1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: IGSF16 knockout HepG2 cell lysate; Predicted MW: 25kd Observed MW: 37kd Primary Ab: 2ug/ml Rabbit Anti-Human IGSF16 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Immunoglobulin Superfamily, Member 16, Polyclonal Antibody (Cat# AAA21021)
Full Name
Immunoglobulin Superfamily, Member 16 (IGSF16) Polyclonal Antibody
Gene Names
CD300C; LIR; CLM-6; CMRF35; IGSF16; CMRF-35; CMRF35A; CMRF-35A; CMRF35A1; CMRF35-A1
Reactivity
Human, Rat, Pig
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
Affinity Chromatography
Pricing
WB (Western Blot)
(Western blot analysis of LoVo cell lysate, using PIBF1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
PIBF1, Polyclonal Antibody (Cat# AAA31130)
Full Name
PIBF1 Antibody
Gene Names
PIBF1; PIBF; CEP90; JBTS33; C13orf24
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of Chk2 phosphorylation expression in Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Chk2, Polyclonal Antibody (Cat# AAA30957)
Full Name
Phospho-Chk2 (Thr387) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U20S cells using anti-Calpastatin antibody (AAA11679).Overlay histogram showing U20S cells stained with AAA11679 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calpastatin Antibody (AAA11679,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Calpastatin, Polyclonal Antibody (Cat# AAA11679)
Full Name
Anti-Calpastatin Antibody
Gene Names
CAST; BS-17; PLACK
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U251 cells using anti-PNP antibody (AAA11680).Overlay histogram showing U251 cells stained with AAA11680 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNP Antibody (AAA11680,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PNP, Polyclonal Antibody (Cat# AAA11680)
Full Name
Anti-PNP Antibody
Gene Names
PNP; NP; PUNP; PRO1837
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 7. IF analysis of CD68 using anti- CD68 antibody CD68 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti- CD68 Antibody overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
CD68, Polyclonal Antibody (Cat# AAA11512)
Full Name
Anti-CD68 antibody
Gene Names
Cd68; Lamp4; gp110; Scard1
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11850)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
Immunohistochemistry, Western Blot
Pricing
streptolysin O IgG, ASO antibody IgG, ELISA Kit (Cat# AAA19124)
Full Name
Human Anti-streptolysin O IgG, ASO antibody IgG ELISA Kit
Reactivity
Human
Pricing
Troponin I, Native Protein (Cat# AAA13523)
Full Name
Troponin I Protein (Active)
Applications
ELISA
Pricing