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3170 results for " Monoclonal Antibodies" - showing 2900-2950
Application Data
(Staining of human peripheral blood granulocytes with CD18: Alexa Fluor 647)
CD18, Monoclonal Antibody (Cat# AAA11881)
Full Name
RAT ANTI HUMAN CD18:FITC
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
Flow Cytometry
Pricing
Application Data
(Staining of human peripheral blood granulocytes with CD18: Alexa Fluor 647)
CD18, Monoclonal Antibody (Cat# AAA11987)
Full Name
RAT ANTI HUMAN CD18
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
Prekallikrein Heavy Chain, Monoclonal Antibody (Cat# AAA14668)
Full Name
Prekallikrein Heavy Chain (Kininogenin, Fletcher factor, KLKB1, KLK3)
Gene Names
KLKB1; PPK; KLK3
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified / Purified by Protein G chromatography.
Pricing
Application Data
(Detection limit for recombinant GST tagged PGR is approximately 0.03ng/ml as a capture antibody.)
PGR, Monoclonal Antibody (Cat# AAA26192)
Full Name
PGR (Progesterone Receptor, NR3C3, PR) (Biotin)
Gene Names
PGR; PR; NR3C3
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27682)
Full Name
VPS35 Antibody, Clone 8A3
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
FCM (Flow Cytometry)
(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FOLH1 antibody, and then analyzed by flow cytometry.)
FOLH1 / PSMA, Monoclonal Antibody (Cat# AAA12376)
Full Name
Anti-FOLH1 / PSMA Antibody (clone 3H5) IHC-plus
Gene Names
FOLH1; PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
Reactivity
Mouse, Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Protein A/G purified
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD40:RPE)
CD40, Monoclonal Antibody (Cat# AAA12090)
Full Name
MOUSE ANTI HUMAN CD40
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry
Pricing
WB (Western Blot)
(SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in IMR-32.)
SMAD1, Monoclonal Antibody (Cat# AAA25927)
Full Name
SMAD1 (SMAD Family Member 1, BSP1, JV4-1, JV41, MADH1, MADR1) (AP)
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Applications
Immunohistochemistry, Western Blot
Purity
Purified
Pricing
WB (Western Blot)
(SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in IMR-32.)
SMAD1, Monoclonal Antibody (Cat# AAA26193)
Full Name
SMAD1 (SMAD Family Member 1, BSP1, JV4-1, JV41, MADH1, MADR1) (Biotin)
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Pricing
Application Data
(Detection limit for recombinant GST tagged PLK1 is ~1ng/ml as a capture antibody.)
PLK1, Monoclonal Antibody (Cat# AAA24321)
Full Name
PLK1 (STPK13, Polo-like Kinase 1, Serine/Threonine-protein Kinase 13, Serine/Threonine-protein Kinase PLK1, PLK, PLK-1) (AP)
Gene Names
PLK1; PLK; STPK13
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:FITC)
CD61, Monoclonal Antibody (Cat# AAA11873)
Full Name
HAMSTER ANTI MOUSE CD61:FITC
Gene Names
Itgb3; CD61; GP3A; INGRB3
Applications
Flow Cytometry
Pricing
IHC (Immunohistochemistry)
(ICH of Macrophage HAM-56 on an FFPE Liver Tissue)
Macrophage HAM-56, Monoclonal Antibody (Cat# AAA13562)
Full Name
Macrophage HAM-56
Reactivity
Human, Monkey
Applications
Immunohistochemistry
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD40:RPE)
CD40, Monoclonal Antibody (Cat# AAA11867)
Full Name
MOUSE ANTI HUMAN CD40:FITC
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
Flow Cytometry
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD40:RPE)
CD40, Monoclonal Antibody (Cat# AAA11936)
Full Name
MOUSE ANTI HUMAN CD40
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Reactivity
Dog
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12197)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
Flow Cytometry
Pricing
Endogenous Retrovirus Type K, Envelope Protein, Monoclonal Antibody (Cat# AAA14674)
Full Name
Endogenous Retrovirus Type K, Envelope Protein (HERV K)
Applications
ELISA
Purity
Purified by Protein G affinity chromatography.
Pricing
Application Data
Glutathione IgG1, Monoclonal Antibody (Cat# AAA14178)
Full Name
anti-Glutathione monoclonal antibody IgG1
Applications
Western Blot, Immunoprecipitation
Purity
Protein A purified.
Pricing
FCM (Flow Cytometry)
(FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)
STIP1, Monoclonal Antibody (Cat# AAA26408)
Full Name
STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (FITC)
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Pricing
IHC (Immunohistochemistry)
(IHC staining of human cerebellar neurons using AAA14700.)
SNAP 25, 26-27kD, Monoclonal Antibody (Cat# AAA14700)
Full Name
SNAP 25, 26-27kD (Synaptosomal-associated Protein)
Reactivity
Rat, Hamster Gerbil and Porcine
Applications
Western Blot, Immunohistochemistry
Purity
Purified by Protein A affinity chromatography from tissue culture supernatent
Pricing
FCM (Flow Cytometry)
(Detection of Carbonic Anhydrase IX/CA9 in U‑87 MG Human Cell Line by Flow Cytometry using AAA14680.)
Carbonic Anhydrase IX, Monoclonal Antibody (Cat# AAA14680)
Full Name
Carbonic Anhydrase IX (CA9) (PE)
Gene Names
CA9; MN; CAIX
Applications
Flow Cytometry
Purity
Affinity Purified
Purified by Protein A affinity chromatography from hybridoma culture supernant.
Purified by Protein A affinity chromatography from hybridoma culture supernant.
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD40:RPE)
CD40, Monoclonal Antibody (Cat# AAA12089)
Full Name
MOUSE ANTI HUMAN CD40:RPE
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12193)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
Flow Cytometry
Pricing
HBsAg, Monoclonal Antibody (Cat# AAA14624)
Full Name
mAb anti-HBsAg, RM-1
Reactivity
Human hepatitis B virus. Others not tested.
Applications
ELISA
Purity
Protein G affinity purified
Pricing
WB (Western Blot)
(SPP1 monoclonal antibody (M09), clone 3E11. Western Blot analysis of SPP1 expression in NIH/3T3 (Cat # L018V1).)
SPP1, Monoclonal Antibody (Cat# AAA26437)
Full Name
SPP1 (Secreted Phosphoprotein 1, BNSP, BSPI, ETA-1, MGC110940, OPN) (HRP)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Applications
Western Blot
Purity
Purified
Pricing
Plasmodium falciparum (Malaria) Histidine-Rich Protein 2 (HRP-2), Monoclonal Antibody (Cat# AAA13396)
Full Name
MAb to P. falciparum HRP-2
Applications
Lateral Flow
Purity
Protein A chromatography
Pricing
Morphine, Monoclonal Antibody (Cat# AAA13372)
Full Name
MAb to Morphine
Applications
Lateral Flow
Purity
Purified, Protein G chromatography
Pricing
IHC (Immunohistochemistry)
(High magnification: Paraffin-embedded human skin tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution of AAA14653. Cells lining up under the epidermis consist of numerous mast cells (The dermis has a moderate diffuse inflammatory cell infiltrate).)
Mast Cell Chymase, Monoclonal Antibody (Cat# AAA14653)
Full Name
Mast Cell Chymase
Gene Names
CMA1; CYH; MCT1; chymase; MGC119890; MGC119891
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified by Protein G affinity chromatography
Pricing
ERO1L, Monoclonal Antibody (Cat# AAA24499)
Full Name
ERO1L (ERO1-like Protein alpha, ERO1-L, ERO1-L-alpha, Endoplasmic Oxidoreductin-1-like Protein, Oxidoreductin-1-L-alpha, UNQ434/PRO865) APC
Gene Names
ERO1A; ERO1L; ERO1-L; ERO1LA; Ero1alpha; ERO1-alpha; ERO1-L-alpha
Reactivity
Human, Rat
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27694)
Full Name
VPS35 Antibody, Clone 10A8: ATTO 594
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Pacific Blue)
CD4, Monoclonal Antibody (Cat# AAA12071)
Full Name
MOUSE ANTI HUMAN CD4:FITC
Gene Names
CD4; CD4mut
Reactivity
Human
Applications
Flow Cytometry
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD40:RPE)
CD40, Monoclonal Antibody (Cat# AAA12031)
Full Name
MOUSE ANTI HUMAN CD40:RPE
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12192)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
Flow Cytometry
Pricing
Application Data
Fumonisin, Monoclonal Antibody (Cat# AAA11720)
Full Name
Fumonisin Monoclonal Antibody
Applications
ELISA
Pricing
FCM (Flow Cytometry)
(FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)
STIP1, Monoclonal Antibody (Cat# AAA26275)
Full Name
STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (Biotin)
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Pricing
WB (Western Blot)
(SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in IMR-32 (Cat # L008V1).)
SMAD1, Monoclonal Antibody (Cat# AAA26459)
Full Name
SMAD1 (SMAD Family Member 1, BSP1, JV4-1, JV41, MADH1, MADR1) (HRP)
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Pricing
Aggrecan, N-terminal neoepitope DIPEN, Monoclonal Antibody (Cat# AAA13857)
Full Name
Monocloncal Antibody DIPEN (Clone BC4)
Gene Names
ACAN; AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified on protein G
Pricing
WB (Western Blot)
(Anti-Mesothelin Antibodies - Immunohistochemistry. Immunohistochemistry using anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN. Reprinted with permission fromClin. Cancer Res. 11(16):5840-6.)
MSLN / Mesothelin, Monoclonal Antibody (Cat# AAA12374)
Full Name
Mouse Monoclonal [clone MN-1] (IgG) to Human MSLN / Mesothelin
Gene Names
MSLN; MPF; SMRP
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Protein A Purified
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12093)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA
Reactivity
Dog, Dolphin, Ferret, Fin Whale, Goat, Horse, Human, Mink, Rabbit, Pig, Sheep.
Based on sequence similarity, is expected to react with: Mustelid
N.B. Antibody reactivity and working conditions may vary between species.
Based on sequence similarity, is expected to react with: Mustelid
N.B. Antibody reactivity and working conditions may vary between species.
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12194)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12198)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
Flow Cytometry
Pricing
WB (Western Blot)
(The following image is the result of using mAb 5B8 ascites at 1:5000 dilution to detect Abeta40 and Abeta42 on Western blot. Lane 1: Abeta42, Lane 2: Abeta40.)
amyloid beta peptide N-terminal, Monoclonal Antibody (Cat# AAA14629)
Full Name
mAb anti-human amyloid beta peptide N-terminus
Reactivity
Human
Applications
Western Blot
Purity
Protein G affinity purified
Pricing
Aggrecan, C-terminal neoepitope NITEGE, Monoclonal Antibody (Cat# AAA13855)
Full Name
Monoclonal Antibody NITEGE (Clone BC13)
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified on protein G
Pricing
CD105, Monoclonal Antibody (Cat# AAA14292)
Full Name
CD105 antibody (biotin)
Reactivity
To be determined by end-user.
Applications
Flow Cytometry, Immunohistochemistry
Purity
>95% pure
CD105 antibody (biotin) was purified by Protein A chromatography.
CD105 antibody (biotin) was purified by Protein A chromatography.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil using AAA14660 and following by peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of mast cells.)
Mast Cell Chymase, Monoclonal Antibody (Cat# AAA14660)
Full Name
Mast Cell Chymase (BSA & Azide Free)
Gene Names
CMA1; CYH; MCT1; chymase; MGC119890; MGC119891
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified by Protein G affinity chromatography.
Pricing
WB (Western Blot)
(HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in PC-12.)
HSPA1L, Monoclonal Antibody (Cat# AAA24833)
Full Name
HSPA1L (Heat Shock 70kD Protein 1-like, HSP70-Hom, Heat shock 70kD protein 1L, Heat Shock 70kD Protein 1-Hom) (Biotin)
Gene Names
HSPA1L; HSP70T; hum70t; HSP70-1L; HSP70-HOM
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27705)
Full Name
VPS35 Antibody, Clone 11H10: FITC
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27685)
Full Name
VPS35 Antibody, Clone 8A3: ATTO 594
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12195)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
Application Data
(Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detaction of aromatase in human tissues by Immunohistochemistry on paraffin sectionsImage caption:Morphology and P450 arom immunoreactivity of tumoral region in human testis with seminoma. A-B: Haematoxylin-eosin staining. C-D: Strong P450 arom immunoreactivity in cytoplasm of neoplastic cells (Nc) and unstained lymphocytes (L). Insert: absorption control. Scale bars: A, 20 um; B-C, 12.5 um; D, 5 um.From: Rago V, Romeo F, Aquila S, Montanaro D, And S, Carpino A. Cytochrome P450 aromatase expression in human seminoma. Reprod Biol Endocrinol. 2005 Dec 22;3:72.)
CYTOCHROME P450 AROMATASE, Monoclonal Antibody (Cat# AAA12113)
Full Name
MOUSE ANTI HUMAN CYTOCHROME P450 AROMATASE
Gene Names
CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12081)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
Western Blot
Pricing