Search results for "Cell Culture"

< >

F4/80, Monoclonal Antibody (Cat# AAA12173)

Full Name

RAT ANTI MOUSE F4/80

Gene Names

Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

Applications

Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot

Pricing

< >

<< Previous Next >>

Application Data (Staining of CD209 transfected K562 cells with MOUSE ANTI HUMAN CD209: FITC)

DC-SIGN, Monoclonal Antibody (Cat# AAA26722)

Full Name

DC-SIGN (Dendritic Cell-specific ICAM3 grabbing Non-integrin, C Type Lectin Domain Family 4 Member L, CLEC4L, CD209, CD209 Antigen, CDSIGN, Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1, DC SIGN1, DC-SIGN1, HIV GP120 Binding Protein, MGC129965) (

Reactivity

Human

Applications

Immunofluorescence

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

< >

<< Previous Next >>

ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TPX2 mAb. Nuclear was stained with Hoechst (blue fluorescence). [Lot No. 3164C6a-1])

TPX2, Monoclonal Antibody (Cat# AAA10610)

Full Name

Mouse monoclonal antibody Anti-Human TPX2

Gene Names

TPX2; DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1

Reactivity

Human

Applications

Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry

Pricing

< >

CD11b, Monoclonal Antibody (Cat# AAA12146)

Full Name

MOUSE ANTI RAT CD11b:FITC

Gene Names

ITGAM; CD11B

Applications

Flow Cytometry

Pricing

IL-6, ELISA Kit (Cat# AAA13517)

Full Name

IL-6 Rat ELISA Kit

Gene Names

Il6; ILg6; Ifnb2

Reactivity

Rat

Applications

ELISA

Pricing

Leptin, ELISA Kit (Cat# AAA13511)

Full Name

Leptin Human ELISA Kit

Gene Names

LEP; OB; OBS; LEPD

Reactivity

Human

Applications

ELISA

Pricing

Catalase, Assay Kit (Cat# AAA27850)

Full Name

Catalase Microplate Assay Kit

Reactivity

General

Applications

Functional Assay

Pricing

< >

CD3, Monoclonal Antibody (Cat# AAA12175)

Full Name

RAT ANTI MOUSE CD3

Gene Names

Cd3e; CD3; T3e; AI504783; CD3epsilon

Applications

Immunohistochemistry, Flow Cytometry

Pricing

< >

<< Previous Next >>

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD45: Pacific Blue)

CD45, Monoclonal Antibody (Cat# AAA12202)

Full Name

MOUSE ANTI HUMAN CD45:FITC

Gene Names

PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180

Applications

Flow Cytometry

Pricing

< >

CD8 ALPHA, Monoclonal Antibody (Cat# AAA11885)

Full Name

RAT ANTI MOUSE CD8 ALPHA:FITC

Gene Names

Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331

Applications

Flow Cytometry

Pricing

< >

<< Previous Next >>

Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . Medium power)

CD13, Monoclonal Antibody (Cat# AAA12115)

Full Name

RAT ANTI MOUSE CD13:RPE

Gene Names

Anpep; Apn; AP-M; AP-N; Cd13; P150

Applications

Flow Cytometry

Pricing

Acetyl-Coa Carboxylase (ACC), ELISA Kit (Cat# AAA18940)

Full Name

Rat Acetyl-Coa Carboxylase (ACC) ELISA Kit

Reactivity

Rat

Pricing

< >

<< Previous Next >>

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD312: Alexa Fluor 488)

CD312, Monoclonal Antibody (Cat# AAA12143)

Full Name

MOUSE ANTI HUMAN CD312:RPE

Gene Names

EMR2; CD312

Applications

Flow Cytometry

Pricing

< >

F4/80, Monoclonal Antibody (Cat# AAA12170)

Full Name

RAT ANTI MOUSE F4/80

Gene Names

Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

Applications

Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot

Pricing

Glucose, Assay Kit (Cat# AAA27844)

Full Name

Glucose (Tissue) Microplate Assay Kit

Reactivity

General

Applications

Functional Assay

Pricing

Neurofilament, Light Polypeptide (NEFL), ELISA Kit (Cat# AAA11398)

Full Name

Human Neurofilament, Light Polypeptide (NEFL) ELISA Kit

Reactivity

Human

Pricing

Transcription intermediary factor 1-beta, TRIM28/KAP1/RNF96/TIF1B, ELISA Kit (Cat# AAA22506)

Full Name

Human Transcription intermediary factor 1-beta, TRIM28/KAP1/RNF96/TIF1B ELISA Kit

Gene Names

TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157

Reactivity

Human

Pricing

Insulin (INS), ELISA Kit (Cat# AAA23116)

Full Name

Mouse Insulin (INS) Mini Samples ELISA Kit

Reactivity

Mouse

Applications

ELISA

Pricing

< >

<< Previous Next >>

Application Data (Staining of NALM6 cells with MOUSE ANTI HUMAN CD10:ALEXA 488)

CD10, Monoclonal Antibody (Cat# AAA26713)

Full Name

CD10 (CALLA, CD 10, Common Acute Lymphocytic Leukemia Antigen, Enkephalinase, gp100, Membrane Metalloendopeptidase, MME, Neprilysin, Neutral Endopeptidase, Pmel17) (AP)

Gene Names

MME; NEP; SFE; CD10; CALLA

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

< >

<< Previous Next >>

ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BRD3 mAb. [Lot No. 2088C3a-1])

BRD3, Monoclonal Antibody (Cat# AAA10603)

Full Name

Mouse monoclonal antibody Anti-Human BRD3

Gene Names

BRD3; ORFX; RING3L

Reactivity

Human

Applications

Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry

Pricing

E-Selectin, ELISA Kit (Cat# AAA13507)

Full Name

E-Selectin Human ELISA Kit

Gene Names

SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2

Reactivity

Human

Applications

ELISA

Pricing

Vitamin K1 (VK1), ELISA Kit (Cat# AAA13014)

Full Name

Human Vitamin K1 (VK1) ELISA kit

Reactivity

Human

Pricing

< >

<< Previous Next >>

Application Data (Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD274:RPE)

CD274, Monoclonal Antibody (Cat# AAA26767)

Full Name

CD274 (CD274 Antigen, B7 Homolog 1, B7H1, B7-H1, B7-H, MGC142294, MGC142296, OTTHUMP00000021029, Programmed Cell Death 1 Ligand 1, PDCD1 Ligand 1, PDCD1L1, PDCD1LG1, Programmed Death Ligand 1, PDL1, PD-L1, RGD1566211) (PE)

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

NEDD8-Activating Enzyme E1 Regulatory Subunit (NAE1), ELISA Kit (Cat# AAA31493)

Full Name

Human NEDD8-Activating Enzyme E1 Regulatory Subunit (NAE1) ELISA Kit

Gene Names

NAE1; HPP1; ula-1; APPBP1; A-116A10.1

Reactivity

Human

Pricing

< >

CD11b, Monoclonal Antibody (Cat# AAA11991)

Full Name

MOUSE ANTI HUMAN CD11b

Gene Names

ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6

Reactivity

Baboon, Cynomolgus monkey, Rhesus Monkey

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Pricing

< >

CD11b, Monoclonal Antibody (Cat# AAA11969)

Full Name

MOUSE ANTI RAT CD11b

Gene Names

ITGAM; CD11B

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation

Pricing

Interleukin 10 (IL10), ELISA Kit (Cat# AAA23099)

Full Name

Rat Interleukin 10 (IL10) High Sensitive ELISA Kit

Gene Names

Il10; IL10X

Reactivity

Rat

Applications

ELISA

Pricing

< >

<< Previous Next >>

Application Data (Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B)

MHC CLASS II RT1B, Monoclonal Antibody (Cat# AAA11977)

Full Name

MOUSE ANTI RAT MHC CLASS II RT1B

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

DNA Damage Inducible Transcript 3, ELISA Kit (Cat# AAA17143)

Full Name

Canine DNA Damage Inducible Transcript 3 ELISA Kit

Gene Names

DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153

Reactivity

Canine

Pricing

< >

<< Previous Next >>

Application Data (Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B)

MHC CLASS II RT1B, Monoclonal Antibody (Cat# AAA11976)

Full Name

MOUSE ANTI RAT MHC CLASS II RT1B

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry

Pricing

< >

<< Previous Next >>

ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-PAX8 mAb. [Lot No. PAX8R1-3])

PAX8, Monoclonal Antibody (Cat# AAA10602)

Full Name

Mouse monoclonal antibody Anti-Human PAX8

Reactivity

Human

Applications

Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry

Pricing

platelet activating factor, PAF, ELISA Kit (Cat# AAA15369)

Full Name

Mouse platelet activating factor, PAF ELISA Kit

Reactivity

Mouse

Pricing

Soluble Urokinase Plasminogen Activator Receptor, ELISA Kit (Cat# AAA27436)

Full Name

Mouse Soluble Urokinase Plasminogen Activator Receptor ELISA Kit

Gene Names

PLAUR; CD87; UPAR; URKR; U-PAR

Reactivity

Mouse

Pricing

< >

F4/80, Monoclonal Antibody (Cat# AAA12171)

Full Name

RAT ANTI MOUSE F4/80:RPE

Gene Names

Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

Applications

Flow Cytometry

Pricing

Pregnancy Associated Plasma Protein A (PAPP-A), ELISA Kit (Cat# AAA13070)

Full Name

Human Pregnancy Associated Plasma Protein A (PAPP-A) ELISA KIT

Reactivity

Human

Pricing

Ovalbumin specific Immunoglobulin E, ELISA Kit (Cat# AAA16530)

Full Name

Mouse Ovalbumin specific Immunoglobulin E ELISA Kit

Reactivity

Mouse

Pricing

< >

<< Previous Next >>

Application Data (Staining of NALM6 cells with MOUSE ANTI HUMAN CD10:ALEXA 488)

CD10, Monoclonal Antibody (Cat# AAA26751)

Full Name

CD10 (CALLA, CD 10, Common Acute Lymphocytic Leukemia Antigen, Enkephalinase, gp100, Membrane Metalloendopeptidase, MME, Neprilysin, Neutral Endopeptidase, Pmel17) (HRP)

Gene Names

MME; NEP; SFE; CD10; CALLA

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

< >

<< Previous Next >>

IP (Immunoprecipitation) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)

BCMA, Monoclonal Antibody (Cat# AAA27972)

Full Name

Anti-BCMA antibody (DM5), Rabbit mAb

Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation, Immunofluorescence

Purity

Purified from cell culture supernatant by affinity chromatography

Pricing

IL-13, ELISA Kit (Cat# AAA13516)

Full Name

IL-13 Rat ELISA Kit

Gene Names

IL13; P600; IL-13

Reactivity

Rat

Applications

ELISA

Pricing

Lipopolysaccharide Binding Protein (LBP), RTU ELISA Kit (Cat# AAA23968)

Full Name

Human Lipopolysaccharide Binding Protein (LBP) ELISA Kit

Gene Names

LBP; BPIFD2

Reactivity

Human

Pricing

Regenerating Islet Derived Protein 1 Alpha (REG1a), ELISA Kit (Cat# AAA11179)

Full Name

Human Regenerating Islet Derived Protein 1 Alpha (REG1a) ELISA Kit

Gene Names

REG1A; P19; PSP; PTP; REG; ICRF; PSPS; PSPS1

Reactivity

Human

Pricing

< >

CD11b, Monoclonal Antibody (Cat# AAA12219)

Full Name

RAT ANTI MOUSE CD11b:Low Endotoxin

Gene Names

Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik

Applications

Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry

Pricing

< >

<< Previous Next >>

Application Data (Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4: Alexa Fluor 488)

CD4, Monoclonal Antibody (Cat# AAA12064)

Full Name

RAT ANTI DOG CD4:FITC

Applications

Flow Cytometry

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Sample: Recombinant PRF1, Human;Antibody: Rabbit Anti-Human PRF1 Ab)

Activity (Perforin 1 (PRF1) is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin binds to the target cell's plasma membrane, and oligomerises in a Ca2+ dependent manner to form pores on the target cell. The pore formed allows for the passive diffusion of a family of pro-apoptotic proteases, known as the granzymes, into the target cell. Besides, Calreticulin (CRT) has been identified as an interactor of PRF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PRF1 and recombinant human CRT. Briefly, PRF1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CRT-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PRF1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PRF1 and CRT was shown in Figure 1, and this effect was in a dose dependent manner.The activity of recombinant PRF1 was measured by lysis of erythrocytes using a hemolysis assay. A general procedure is as fllows: two-fold dilute the recombinant human PRF1 with 0.9% NaCl, add 50uL a serial dilution of PRF1, 10uL 0.1M CaCl2 to each well, then add 50uL 0.25% rabbit erythrocyte (RaE) to each well and mixed gently. Add 10uL 0.9% NaCl to reaplace CaCl2 in control wells. The plate is incubated for 20 hours at 37 degree C, 5% CO2. The results are shown in Figure 2. It was obvious that the minimal effective concentration of PRF1 is 12.5ug/mL.)