Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
287 results for " Viral" - showing 200-250
SDS-PAGE
(4-12% Bis-Tris NuPAGE gel showing the following lanes:1- MW standard;2- IgG3 (5 ug reduced/heated);3- IgG3 (10 ug reduced/heated);4- IgG3 (20 ug reduced/heated);5- IgG3 (5 ug non-reduced/no heat);6- IgG3 (10 ug non-reduced/no heat);7- IgG3 (20 ug non-reduced/no heat))
Human IgG3 protein, Immunoglobulin (Cat# AAA14399)
Full Name
Human IgG3 protein
Purity
> 95% pure by SDS-PAGE
Pricing
SDS-PAGE
(SDS-PAGE (4-20%): purified full-length spike (aa 16-1208) protein (SARS-CoV-2))
COVID 19 Spike Protein Coronavirus, Recombinant Protein (Cat# AAA13788)
Full Name
Spike Protein of SARS-CoV-2 (COVID-19)
Applications
Western Blot
Purity
>95% (by SDS PAGE)
Pricing
SDS-PAGE
(SDS-PAGE: purified VIsE protein of Borrelia burgdorferi from 293 cells)
VlsE protein of Borrelia burgdorferi, Recombinant Protein (Cat# AAA13692)
Full Name
Recombinant VlsE protein of Borrelia burgdorferi
Applications
Western Blot
Purity
>= 95% (by SDS-PAGE)
Pricing
Application Data
(SDS-PAGE Data: purified HA1 (H5N1)(A/Hong Kong/156/97) protein)
H5 (H5N1) (A/Hong Kong/156/97), Recombinant Protein (Cat# AAA13749)
Full Name
HA (18-346)(H5N1)(A/Hong Kong/156/97)
Applications
Western Blot
Purity
> 95% pure by 10% SDS-PAGE gel
Pricing
Dengue Virus Subtype 4 Envelope 45kDa, Recombinant Protein (Cat# AAA10879)
Full Name
Recombinant Dengue Virus Subtype 4 Envelope 45kDa
Purity
Protein is >95% pure as determined by 12% PAGE (coomassie staining).
Purified by proprietary chromatographic technique.
Purified by proprietary chromatographic technique.
Pricing
IHC (Immunohistchemistry)
(Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.)
c-FOS, Antibody (Cat# AAA13673)
Full Name
Goat anti-c-FOS (aa283-295) Antibody
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
Applications
Peptide ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
(Western blot analysis of EGFR phosphorylation expression in 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
EGFR, Polyclonal Antibody (Cat# AAA30962)
Full Name
Phospho-EGFR (Ser695) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
SDS-PAGE
(SDS-PAGE: purified full length envelope protein of Zika virus)
Zika Virus Envelope Protein, Recombinant Protein (Cat# AAA13773)
Full Name
Recombinant envelope protein of Zika virus
Applications
Western Blot
Purity
>95% purity
Pricing
SDS-PAGE
(SDS-PAGE: purified HA (A/Hong Kong/45/2019)(H3N2) protein)
HA (A/Hong kong/45/2019) (H3N2)-like virus, Recombinant Protein (Cat# AAA13781)
Full Name
HA (A/Hong kong/45/2019) (H3N2)-like virus
Applications
Western Blot
Purity
>95% pure by 12% SDS PAGE gel
Pricing
Application Data
(The HAV Antigen can be used in a direct ELISA for the detection of either IgG or IgM antibodies. The HAV antigen has demonstrated reactivity with antibodies of individuals vaccinated with the HAVRIX vaccine.)
Hepatitis A Virus, pHM175, Infectious Disease (Cat# AAA14849)
Full Name
Hepatitis A Virus, pHM175 (HAV)
Applications
Western Blot
Purity
Purified
Pricing
COVID 19 Nucleocapsid (NP) Humanized Coronavirus, Monoclonal Antibody (Cat# AAA13544)
Full Name
Anti-SARS-CoV-2 Nucleocapsid / COVID-19, Humanized antibody
Reactivity
Viral
Applications
Lateral Flow
Purity
Purity: >95% by HPLC & SDS-PAGE
Purification: Protein A purified
Purification: Protein A purified
Pricing
IHC (Immunohistochemistry)
(AAA31083 at 1/100 staining human breast tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 92)
EGFR, Polyclonal Antibody (Cat# AAA31083)
Full Name
EGFR Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
Application Data
(SDS-PAGE Purified NP (H5N1)(A/Indonesia/5/05) protein.)
NP (H5N1) (A/Indonesia/5/2005), Recombinant Protein (Cat# AAA13755)
Full Name
NP (H5N1) (A/Indonesia/5/2005)
Applications
Western Blot
Purity
> 95% (SDS-PAGE)
Pricing
Sequence
Membrane Glycoprotein G1 of Phlebovirus, Recombinant Protein (Cat# AAA13772)
Full Name
Membrane Glycoprotein G1 of Phlebovirus (SFTS virus HN6)
Applications
Western Blot
Purity
> 95% purity by SDS-PAGE gel
Pricing
Application Data
c-Src (Tyr-215), Polyclonal Antibody (Cat# AAA14100)
Full Name
c-Src (Tyr-215), phospho-specific [Conserved site]
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, Rat, Mouse
Applications
Western Blot
Pricing
IF (Immunofluorescence)
(AAA31071 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
HER4, Polyclonal Antibody (Cat# AAA31071)
Full Name
Phospho-HER4 (Tyr1284) Antibody
Gene Names
ERBB4; HER4; ALS19; p180erbB4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(SDS-PAGE: purified H5 (H5N1) (A/Anhui/1/2005) protein (reducing condition))
H5 (H5N1) (A/Anhui/1/2005), Recombinant Protein (Cat# AAA13760)
Full Name
H5 (H5N1) (A/Anhui/1/2005) (aa 18-530)
Applications
Western Blot
Purity
> 95% pure by 10% SDS-PAGE gel
Pricing
COVID 19 Nucleocapsid (NP) IgM Humanized Coronavirus, Monoclonal Antibody (Cat# AAA13546)
Full Name
Anti-SARS-CoV-2 Nucleocapsid IgM/COVID-19, Humanized antibody (Positive Control)
Reactivity
Viral
Applications
Lateral Flow
Purity
Purity: >95% by HPLC & SDS-PAGE
Purification: Protein A purified
Purification: Protein A purified
Pricing
Affinity Assay
(The binding between immobilized S1RBD and the anti-SARS-CoV-2 S1RBD AAA13616)
COVID 19 S1 Spike RBD Coronavirus, Antibody (Cat# AAA13616)
Full Name
Humanized Monoclonal Antibody against SARS-CoV-2 Spike Protein-1 Receptor-binding Domain (S1 RBD)
Reactivity
Viral
Applications
ELISA
Pricing
Application Data
(SDS-PAGE(12%): purified E2 (HCV)(aa 383-663), subtype 1b)
E2 (HCV), Recombinant Protein (Cat# AAA13742)
Full Name
E2 (HCV) (amino acid 383-663), subtype 1b
Applications
Western Blot
Purity
> = 96% purity
Pricing
SDS-PAGE
(SDS-PAGE: purified HIV-1 gp120 (Bal) protein)
gp120 (HIV-1/Clade B), Recombinant Protein (Cat# AAA13743)
Full Name
gp120 (HIV-1/Clade B) (amino acid 24-518)
Applications
Western Blot
Purity
> 95% purity (SDS-PAGE)
Pricing
PDGF, Recombinant Protein (Cat# AAA12302)
Full Name
RECOMBINANT HUMAN PDGF BB HOMODIMER
Gene Names
PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
Applications
Functional Assay, Western Blot
Purity
>98% by SDS PAGE and HPLC analysis
Pricing
SDS-PAGE
(SDS-PAGE: purified HA1 (H1N1)(A//Puerto Rico/8/1934) protein)
HA1 (H1N1)(A/PR/8/34), Recombinant Protein (Cat# AAA13748)
Full Name
HA1 (H1N1) (aa 18-345)(A/PR/8/34)
Applications
Western Blot
Purity
> 95% by SDS PAGE
Pricing
CMV Pp65 (UL83), Recombinant Protein (Cat# AAA14179)
Full Name
CMV Pp65 (UL83) recombinant antigen, full length
Reactivity
Strongly reacts with human CMV positive serum.
Applications
Western Blot
Purity
>90% pure; Purity of proteins is evaluated by SDS-PAGE, by measuring optical density at 280nm and by method of Bradford et al.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
IRF7, Polyclonal Antibody (Cat# AAA31330)
Full Name
Phospho-IRF7 (Ser471/Ser472) Antibody
Gene Names
IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
SDS-PAGE
(SDS-PAGE: purified HIV-1 gp120 (HXBc2) (Clade B) protein)
gp120 (HXBc2) (HIV-1/Clade B), Recombinant Protein (Cat# AAA13751)
Full Name
gp120 (HXBc2) (HIV-1/Clade B) (aa 34-518)
Applications
Western Blot
Purity
> 95% purity (SDS PAGE).
Pricing
SDS-PAGE
(SDS-PAGE: purified HA (H3N2)(A/Texas/50/2012) protein)
HA1 (A/Texas/50/2012)(H3N2), Recombinant Protein (Cat# AAA13765)
Full Name
HA1(H3N2)(A/Texas/50/2012)(aa 17-529)
Applications
Western Blot
Purity
>95% pure by 10% SDSPAGE gel
Pricing
WB (Western Blot)
(Western blot analysis of Akt phosphorylation expression in B2C Cell line lysates, The lane on the right is treated with the antigen-specific peptide.)
Akt, Polyclonal Antibody (Cat# AAA31035)
Full Name
Phospho-Akt (Ser124) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
SDS-PAGE
(SDS-PAGE:purified HA(aa 18-529)(A/Michigan/45/2015)(H1N1) protein;A, 1ug HA; B, 2ug HA)
Hemagglutinin HA (A/Michigan/45/2015) (H1N1), Recombinant Protein (Cat# AAA13696)
Full Name
Hemagglutinin HA (A/Michigan/45/2015) (H1N1) (aa 18-529)
Applications
Western Blot
Purity
>95% pure by 12% SDS PAGE gel
Pricing
SDS-PAGE
M1 (A/California/04/2009)(H1N1), Recombinant Protein (Cat# AAA13771)
Full Name
M1 (A/California/04/2009)(H1N1)
Applications
Western Blot
Purity
>95% pure by 10% SDS PAGE gel
Pricing
CYTOMEGALOVIRUS, Antigen (Cat# AAA12310)
Full Name
NATIVE CYTOMEGALOVIRUS
Applications
ELISA
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-KIT (Tyr703) expression in various lysates)
KIT, Polyclonal Antibody (Cat# AAA31006)
Full Name
Phospho-KIT (Tyr703) Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117; MASTC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12081)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
Western Blot
Pricing
Dengue Virus Subtype 1 Envelope 45kDa, Recombinant Protein (Cat# AAA10878)
Full Name
Recombinant Dengue Virus Subtype 1 Envelope 45kDa
Applications
Immunoassay
Purity
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Purified by proprietary chromatographic technique.
Purified by proprietary chromatographic technique.
Pricing
HBV core, Recombinant Protein (Cat# AAA14181)
Full Name
HBV core recombinant antigen HBcAg a.a 1 to a.a 183 of HBV core antigen 18 kDa
Applications
Immunoprecipitation, Western Blot, Lateral Flow
Purity
>95% pure: Purity of proteins is evaluated by SDS-PAGE, by measuring optical density at 280nm and by method of Bradford et al.
Pricing
LCMV, Monoclonal Antibody (Cat# AAA14287)
Full Name
LCMV antibody
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Culture supernatant
Pricing
HBcAg, Recombinant Protein (Cat# AAA14357)
Full Name
HBcAg protein
Applications
Western Blot
Purity
>95 % (by SDS-PAGE)
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
NFkappaB p65, Monoclonal Antibody (Cat# AAA29642)
Full Name
NFkappaB p65 Mouse Monoclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification using immunogen.
Pricing
IHC-P (Immunohistochemistry-Paraffin)
(At 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediatedantigen retrieval step in citrate buffer was performed. Thetissue was then blocked and incubated with the antibody for1.5 hours at 22°C. An HRP conjugated goat anti-rabbitantibody was used as the secondary antibody.)
c-Kit, Polyclonal Antibody (Cat# AAA31007)
Full Name
Phospho-c-Kit (Tyr721) Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117; MASTC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
Application Data
(PE conjugatedMouse anti Human CD169 antibody, clone 7-239 used to block CD169 function on myeloid cells.Image caption:Siglec-1 mediates HIV-1 uptake into a storage compartment and enhances HIV-1 trans-infection specially in IFN?-treated monocytes and DCs. A. Uptake of HIV-1NL4–3 by different myeloid cells exposed to IFN?. Cells were cultured with HIV-1 to measure p24Gag by ELISA. Mean values and SEM from four experiments include cells from 12 donors. B. Fold change in HIV-1NL4–3 uptake of cells treated with bafilomycin A1 compared to untreated cells. Mean values and SEM include cells from three donors. C. Relative uptake of HIV-1NL4–3 by IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 uptake by mock-treated cells (set at 100%). Mean values and SEM from two experiments include cells from six donors. D. Confocal microscopy analysis of different IFN?-treated myeloid cells pulsed with HIV-1Cherry and stained for Siglec-1 (Alexa 488), HLA-DR (Alexa 647) and DAPI. (Top) Representative viral pattern for each kind of myeloid cell analyzed, showing maximum fluorescence intensity of four channels. (Bottom) Percentage of myeloid cells with distinct viral patterns: random distribution, polarized accumulation, and sac-like compartment formation, as illustrated in the left drawing. Mean values of 50 cells from two different donors are shown. E. HIV-1 transmission from IFN?-treated myeloid cells to a luciferase reporter CD4+ cell line. HIV-1 infection was determined by induced luciferase activity in relative light units (RLUs). Mean values and SEM from four experiments include cells from 12 donors. F. Relative HIV-1 transmission from IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 trans-infected by mock-treated cells. Mean values and SEM from two experiments include cells from six donors. Statistical differences were assessed with a paired t test in A and E, and with a one sample t-test in B, C and F.From: Pino M, Erkizia I, Benet S, Erikson E, Fernández-Figueras MT, Guerrero D, Dalmau J, Ouchi D, Rausell A, Ciuffi A, Keppler OT, Telenti A, Kräusslich HG, Martinez-Picado J, Izquierdo-Useros N.HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells.Retrovirology. 2015 May 7;12:37.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
CD169, Monoclonal Antibody (Cat# AAA12265)
Full Name
Mouse Anti Human CD169: RPE
Gene Names
SIGLEC1; SN; CD169; SIGLEC-1
Reactivity
Human
Applications
Flow Cytometry
Purity
>95% by SDS PAGE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various celllines, using Phospho-Src (Tyr529) Antibody.)
Src, Polyclonal Antibody (Cat# AAA31009)
Full Name
Phospho-Src (Tyr530) Antibody
Gene Names
SRC; ASV; SRC1; THC6; c-SRC; p60-Src
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
SDS-PAGE
(SDS-PAGE: Envelope (West Nile Virus) protein)
E (DIII) (WNV), Recombinant Protein (Cat# AAA13741)
Full Name
West Nile Virus E (DIII) Protein
Applications
Western Blot
Purity
> 95% purity (SDS-PAGE)
Pricing
Double-stranded RNA (dsRNA), Monoclonal ELISA Kit (Cat# AAA14576)
Full Name
Double-stranded RNA (dsRNA) ELISA kit (J2 based)
Reactivity
General
Applications
ELISA
Pricing
SDS-PAGE
(4-20% SDS-PAGE: purified gH/gp42(EBV) protein complex)
EBV (HHV-4) gH/gp42, Recombinant Protein (Cat# AAA13703)
Full Name
EBV (HHV-4) gH/gp42 Protein Complex
Applications
Western Blot
Purity
>95% pure by 10% SDS-PAGE gel
Pricing
HIV-1 tat, Recombinant Protein (Cat# AAA14180)
Full Name
HIV-1 TAT antigen (recombinant) full length 14 kDa
Purity
> 95% pure
Purity of proteins is evaluated by SDS-PAGE by measuring optimal density at 280 nm.
Purity of proteins is evaluated by SDS-PAGE by measuring optimal density at 280 nm.
Pricing
Affinity Assay
(The binding between immobilized S1RBD and the anti-SARS-CoV-2 S1RBD AAA13617.)
COVID 19 S1 Spike RBD Coronavirus, Antibody (Cat# AAA13617)
Full Name
Humanized Monoclonal Antibody against SARS-CoV-2 Spike Protein-1 Receptor-binding Domain (S1 RBD)
Reactivity
Viral
Applications
ELISA
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various tissue sample, using Src Antibody.)
Src, Polyclonal Antibody (Cat# AAA31086)
Full Name
Src Antibody
Gene Names
SRC; ASV; SRC1; THC6; c-SRC; p60-Src
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of EGFR phosphorylation expression in TSA treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
EGFR, Polyclonal Antibody (Cat# AAA30963)
Full Name
Phospho-EGFR (Ser1026) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30920 at 1/200 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Akt, Polyclonal Antibody (Cat# AAA30920)
Full Name
Phospho-Akt(Ser473) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11864)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
Immunofluorescence
Pricing