Search results for " Mouse IgG"

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IHC (Immunohistochemistry-Paraffin) (AAA31201 at 1/100 staining Human lung cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4Â°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

PICALM, Polyclonal Antibody (Cat# AAA31201)

Full Name

PICALM Antibody

Gene Names

PICALM; LAP; CALM; CLTH

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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IHC (Immunohistchemistry-Paraffin) (AAA31252 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4Â°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

KDM5C/Jarid1C/SMCX, Polyclonal Antibody (Cat# AAA31252)

Full Name

KDM5C/Jarid1C/SMCX Antibody

Gene Names

KDM5C; MRXJ; SMCX; MRX13; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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Filamin A, Polyclonal Antibody (Cat# AAA31022)

Full Name

Phospho-Filamin A (Ser2152) Antibody

Gene Names

FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FGS2; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

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IF (Immunofluorescence) (AAA31253 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31253 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Connexin 40/GJA5, Polyclonal Antibody (Cat# AAA31253)

Full Name

Connexin 40/GJA5 Antibody

Gene Names

GJA5; CX40; ATFB11

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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IF (Immunofluorescence) (AAA31189 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31189 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Lgi1, Polyclonal Antibody (Cat# AAA31189)

Full Name

Lgi1 Antibody

Gene Names

LGI1; EPT; ETL1; ADLTE; ADPAEF; ADPEAF; IB1099; EPITEMPIN

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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IF (Immunofluorescence) (AAA31195 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31195 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

P2X3, Polyclonal Antibody (Cat# AAA31195)

Full Name

P2X3 Antibody

Gene Names

P2RX3; P2X3

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(88%), Dog(100%)

Applications

Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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IF (Immunofluorescence) (AAA31116 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31116 1:200) and mouse antibeta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)

PLCB2, Polyclonal Antibody (Cat# AAA31116)

Full Name

PLCB2 Antibody

Gene Names

PLCB2; PLC-beta-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific)

Pricing

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CD4, Monoclonal Antibody (Cat# AAA11995)

Full Name

MOUSE ANTI RAT CD4 (DOMAIN 1)

Gene Names

Cd4; p55; W3/25

Applications

Immunohistochemistry, Flow Cytometry, Immunohistochemistry

Pricing

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IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

ENSA, Polyclonal Antibody (Cat# AAA31358)

Full Name

Phospho-ENSA (Ser67/Ser62) Antibody

Gene Names

ENSA; ARPP-19e

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (AAA31079 at 1/200 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

MCL1, Polyclonal Antibody (Cat# AAA31079)

Full Name

MCL1 Antibody

Gene Names

MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

EZH2, Polyclonal Antibody (Cat# AAA31470)

Full Name

Phospho-EZH2 (Thr367) Antibody

Gene Names

EZH2; WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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CD1a, Monoclonal Antibody (Cat# AAA12200)

Full Name

MOUSE ANTI HUMAN CD1a:RPE

Gene Names

CD1A; R4; T6; CD1; FCB6; HTA1

Applications

Flow Cytometry

Pricing

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Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Estrogen Receptor, alpha, Monoclonal Antibody (Cat# AAA23915)

Full Name

Estrogen Receptor, alpha (Marker of Estrogen Dependence)

Gene Names

ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1

Reactivity

Human

Applications

Immunofluorescence, Flow Cytometry, Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (AAA11663).Lipocalin 2 was detected in frozen section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Lipocalin 2 Antibody (AAA11663) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Lipocalin 2, Polyclonal Antibody (Cat# AAA11663)

Full Name

Anti-Lipocalin 2 Antibody

Gene Names

Lcn2; Sip24

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen Affinity Purified

Pricing

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Staining Procedure (1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides.2. Air dry for 2 hours at 58° C.3. Deparaffinize, dehydrate and rehydrate tissues.4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.5. Any of three heating methods may be used:a. TintoRetriever Pressure Cooker or EquivalentPlace tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. TintoRetriever PT Module or Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.c. Conventional Steamer MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. 8. Wash slides with ImmunoDNA washer or DI water. 9. Continue IHC staining protocol.)

Podoplanin/D2-40, Monoclonal Antibody (Cat# AAA13558)

Full Name

Podoplanin/D2-40

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry

Pricing

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Akt, Polyclonal Antibody (Cat# AAA31035)

Full Name

Phospho-Akt (Ser124) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (Anti-Mesothelin Antibodies - Immunohistochemistry. Immunohistochemistry using anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN. Reprinted with permission fromClin. Cancer Res. 11(16):5840-6.)

MSLN / Mesothelin, Monoclonal Antibody (Cat# AAA12374)

Full Name

Mouse Monoclonal [clone MN-1] (IgG) to Human MSLN / Mesothelin

Gene Names

MSLN; MPF; SMRP

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Protein A Purified

Pricing

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CD8 ALPHA, Monoclonal Antibody (Cat# AAA11983)

Full Name

MOUSE ANTI RAT CD8 ALPHA

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot

Pricing

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DECTIN-1, Monoclonal Antibody (Cat# AAA12134)

Full Name

RAT ANTI MOUSE DECTIN-1

Gene Names

Clec7a; BGR; beta-GR; Clecsf12

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Pricing

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Application Data (Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)

IFN GAMMA, Monoclonal Antibody (Cat# AAA12093)

Full Name

MOUSE ANTI BOVINE INTERFERON GAMMA

Reactivity

Dog, Dolphin, Ferret, Fin Whale, Goat, Horse, Human, Mink, Rabbit, Pig, Sheep.
Based on sequence similarity, is expected to react with: Mustelid
N.B. Antibody reactivity and working conditions may vary between species.

Applications

Flow Cytometry

Pricing

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Application Data (Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)

Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12194)

Full Name

RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

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Application Data (Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)

Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12198)

Full Name

RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE

Applications

Flow Cytometry

Pricing

AP-1, Polyclonal Antibody (Cat# AAA22055)

Full Name

JUN Polyclonal Antibody

Gene Names

AP-1gamma; Ap-1; AP-1; AP-1 gamma; AP-1[gamma]; AP1gamma; AP1gamma1; apl4; cg9113; CG9113; dgamma; DmelCG9113; gamma; gamma-Ada

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

p-PERK, Polyclonal Antibody (Cat# AAA29728)

Full Name

p-PERK (Phospho-Thr 982) Antibody

Gene Names

EIF2AK3; PEK; WRS; PERK

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity purification using immunogen.

Pricing

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FCM (Flow Cytometry) (Human HepG2 cell was fixed with 2% paraformaldehyde (10 min), permeabilized with 0.1% BSA-Triton X-100,then stained with 20ug/ml rabbit Anti-human APOC1 Polyclonal Antibody (Catalog AAA20073, red histogram) or Isotype control antibody (Catalog green histogram), followed by 1ug/ml FITC-conjugated Anti-rabbit IgGSecondary Antibody (Catalog (Immunofluorescence))

Apolipoprotein C1 (APOC1), Antibody (Cat# AAA20073)

Full Name

Polyclonal Antibody to Apolipoprotein C1 (APOC1)

Gene Names

APOC1; Apo-CI; ApoC-I; apo-CIB; apoC-IB

Reactivity

Human, Mouse

Applications

WB, IHC, ICC, IF, FCM

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil.)

CCR2, Monoclonal Antibody (Cat# AAA30174)

Full Name

CCR2 Antibody

Gene Names

CCR2; CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

Affinity-chromatography

Pricing

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CD102, Monoclonal Antibody (Cat# AAA11904)

Full Name

RAT ANTI MOUSE CD102:Low Endotoxin

Gene Names

Icam2; CD102; Ly-60; Icam-2

Applications

Flow Cytometry, Functional Assay, Immunoprecipitation

Pricing

TNFR1/CD120a/TNFRSF1A, Monoclonal Antibody (Cat# AAA27740)

Full Name

TNFR1/CD120a/TNFRSF1A Neutralizing Antibody

Gene Names

TNFRSF1A; FPF; MS5; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1-d2

Applications

Neutralization

Pricing

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IHC (Immunohistchemistry-Paraffin) (AAA31218 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4Â°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Siglec 7, Polyclonal Antibody (Cat# AAA31218)

Full Name

Siglec 7 Antibody

Gene Names

SIGLEC7; p75; QA79; AIRM1; CD328; CDw328; D-siglec; SIGLEC-7; SIGLECP2; SIGLEC19P; p75/AIRM1

Reactivity

Human, Mouse, Rat

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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IHC (Immunohistochemistry) (AAA31119 at 1/100 staining mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.)

GPRC5A, Polyclonal Antibody (Cat# AAA31119)

Full Name

GPRC5A Antibody

Gene Names

GPRC5A; RAI3; TIG1; RAIG1; GPCR5A; PEIG-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunofluorescence, Immunocytochemistry

Purity

Peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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OVOL2, Polyclonal Antibody (Cat# AAA31236)

Full Name

OVOL2 Antibody

Gene Names

OVOL2; CHED; CHED1; CHED2; PPCD1; ZNF339; EUROIMAGE566589

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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IF (Immunofluorescence) (AAA31178 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31178) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

CENPB, Polyclonal Antibody (Cat# AAA31178)

Full Name

CENPB Antibody

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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CD4, Monoclonal Antibody (Cat# AAA11994)

Full Name

MOUSE ANTI RAT CD4 (DOMAIN 1)

Gene Names

Cd4; p55; W3/25

Applications

Immunohistochemistry, Flow Cytometry, Immunohistochemistry

Pricing

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IF (Immunofluorescence) (AAA31193 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31193 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

CHD4, Polyclonal Antibody (Cat# AAA31193)

Full Name

CHD4 Antibody

Gene Names

CHD4; CHD-4; Mi-2b; SIHIWES; Mi2-BETA

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Applications

Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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IF (Immunofluorescence) (AAA31215 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31215 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

PDE11A, Polyclonal Antibody (Cat# AAA31215)

Full Name

PDE11A Antibody

Gene Names

PDE11A; PPNAD2

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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IF (Immunofluorescence) (AAA31225 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31225) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

KCNK9, Polyclonal Antibody (Cat# AAA31225)

Full Name

KCNK9 Antibody

Gene Names

KCNK9; KT3.2; TASK3; K2p9.1; TASK-3

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Chicken(83%), Xenopus(83%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

ZO 1, Polyclonal Antibody (Cat# AAA31075)

Full Name

ZO 1 Antibody

Gene Names

TJP1; ZO-1

Reactivity

Human, Mouse, Rat, Pig, Monkey

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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IF (Immunofluorescence) (AAA31141 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31141 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)

MUC5B, Polyclonal Antibody (Cat# AAA31141)

Full Name

MUC5B Antibody

Gene Names

MUC5B; MG1; MUC5; MUC9; MUC-5B

Reactivity

Human

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

Peptide affinity purification

Pricing

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CD1a, Monoclonal Antibody (Cat# AAA12011)

Full Name

MOUSE ANTI HUMAN CD1a

Gene Names

CD1A; R4; T6; CD1; FCB6; HTA1

Reactivity

Cynomolgus monkey, Dog

Applications

Immunohistochemistry, Flow Cytometry

Pricing

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WB (Western Blot) (Western blot analysis on mouse liver tissue lysate using E-cadherin Antibody)

E-cadherin, Polyclonal Antibody (Cat# AAA30922)

Full Name

E-cadherin Antibody

Gene Names

CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

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MAP2, Polyclonal Antibody (Cat# AAA31338)

Full Name

MAP2 Antibody

Gene Names

MAP2; MAP2A; MAP2B; MAP2C

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

Peptide affinity purification

Pricing

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Application Data (Sandwich ELISA analysis of CD178 binding using Mouse anti Human CD178 as a capture reagent and biotinylated Mouse anti Human CD178 as a detection reagent with purified human CD178 as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Plasma (orange) sample is displayed at 1:2 dilution.)

CD178, Monoclonal Antibody (Cat# AAA11962)

Full Name

MOUSE ANTI HUMAN CD178

Gene Names

FASLG; APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1

Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation

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KIT, Polyclonal Antibody (Cat# AAA31006)

Full Name

Phospho-KIT (Tyr703) Antibody

Gene Names

KIT; PBT; SCFR; C-Kit; CD117; MASTC

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

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IHC (Immunohistchemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IRAK1, Polyclonal Antibody (Cat# AAA31368)

Full Name

Phospho-IRAK1 (Thr209) Antibody

Gene Names

IRAK1; IRAK; pelle

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Horse (100%), Dog (82%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

Application Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12232)

Full Name

RAT ANTI TUBULIN ALPHA:HRP

Applications

Immunohistochemistry, Western Blot

Pricing

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Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

Application Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)