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2304 results for " secondary" - showing 2200-2250


product-image-AAA11603_FCM7.jpg FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Hela cells using anti-Calbindin antibody (AAA11603).Overlay histogram showing Hela cells stained with AAA11603 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calbindin Antibody (AAA11603,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Calbindin, Polyclonal Antibody (Cat# AAA11603)

Full Name
Anti-Calbindin Antibody
Gene Names
CALB1; CALB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
product-image-AAA28729_IHC6.jpg IHC (Immunohistchemistry) (Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (L176), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry;clinical relevance has not been evaluated.)

ATG16L, Polyclonal Antibody (Cat# AAA28729)

Full Name
ATG16L Antibody
Gene Names
ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
Reactivity
Human, mouse
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA12314_WB2.jpg WB (Western Blot) (Western blot of BAFF/BLyS in mouse and human cell lysates. Lane A: mouse testis, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human).)

BAFF, Polyclonal Antibody (Cat# AAA12314)

Full Name
Rabbit Polyclonal (IgG) to Human BAFF
Gene Names
TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Protein G Purified
Pricing
product-image-AAA31042_WB20.jpg WB (Western Blot) (Western blot analysis of STAT3 phosphorylation expression in HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

STAT3, Polyclonal Antibody (Cat# AAA31042)

Full Name
Phospho-STAT3 (Ser727) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA14897_APP.jpg Application Data

Mouse Anti-Human IgG (Fc) (gamma chain specific), Monoclonal Secondary Antibody (Cat# AAA14897)

Full Name
Mouse Anti-Human IgG (Fc)-HRP
Applications
ELISA
Pricing
product-image-AAA26976_WB.jpg WB (Western Blot) (Western BlotPositive WB detected in Recombinant proteinAll lanes: ompK antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionpredicted band size: 44 KDaobserved band size: 44 KDa)

ompK, Polyclonal Antibody (Cat# AAA26976)

Full Name
ompK Antibody
Reactivity
Vibrio parahaemolyticus serotype O3: K6
Applications
Western Blot
Purity
>95%, Protein G purified
Pricing
product-image-AAA30944_IHC13.jpg IHC (Immunohistochemistry) (AAA30944 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

HSP20, Polyclonal Antibody (Cat# AAA30944)

Full Name
Phospho-HSP20 (Ser16) Antibody
Gene Names
HSPB6; HEL55; Hsp20; PPP1R91
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA30932_IF3.jpg IF (Immunofluorescence) (AAA30932 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA30932 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)

TGF beta1, Polyclonal Antibody (Cat# AAA30932)

Full Name
TGF beta1 Antibody
Gene Names
TGFB1; CED; LAP; DPD1; TGFB; TGFbeta
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA30940_IF14.jpg IF (Immunofluorescence) (AAA30940 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

GSK3 beta, Polyclonal Antibody (Cat# AAA30940)

Full Name
Phospho-GSK3 beta (Ser9) Antibody
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
Peptide affinity purification
Pricing
product-image-AAA28087_IF8.jpg IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using GNA13 Polyclonal Antibody (AAA28087) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

GNA13, Polyclonal Antibody (Cat# AAA28087)

Full Name
GNA13 Polyclonal Antibody
Gene Names
GNA13; G13
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
product-image-AAA28798_WB.jpg WB (Western Blot) (Anti-Zebrafish hey2 Antibody (C-term)at 1:2000 dilution + ZF4 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

hey2, Polyclonal Antibody (Cat# AAA28798)

Full Name
Zebrafish hey2 Antibody (C-term)
Gene Names
hey2; grl; zgc:136746
Reactivity
Zebrafish
Applications
Western Blot
Pricing
product-image-AAA26962_IHC3.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human pancreatic tissue using AAA26962 at dilution 1:100)

BTNL2, Polyclonal Antibody (Cat# AAA26962)

Full Name
BTNL2 Antibody
Gene Names
BTNL2; SS2; BTN7; BTL-II; HSBLMHC1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
>95%, Protein G purified
Pricing
product-image-AAA26933_IF5.jpg IF (Immunofluorescence) (Immunofluorescent analysis of Hela cells cells using AAA26933 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

S1PR1, Polyclonal Antibody (Cat# AAA26933)

Full Name
S1PR1 Antibody
Gene Names
S1PR1; EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
product-image-AAA26963_IF4.jpg IF (Immunofluorescence) (Immunofluorescent analysis of HepG2 cells using AAA26963 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

PRDX2, Polyclonal Antibody (Cat# AAA26963)

Full Name
PRDX2 Antibody
Gene Names
PRDX2; PRP; TSA; PRX2; PTX1; TPX1; NKEFB; PRXII; TDPX1; NKEF-B; HEL-S-2a
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
product-image-AAA28691_WB6.jpg WB (Western Blot) (DRD2 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DRD2 antibody detected the DRD2 protein (arrow).)

DRD2, Polyclonal Antibody (Cat# AAA28691)

Full Name
DRD2 Antibody (C-term)
Gene Names
DRD2; D2R; D2DR
Reactivity
Human, mouse
Applications
Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA28228_IF6.jpg IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cell using MMP16 antibody (AAA28228) at dilution of 1:100. Blue: DAPI for nuclear staining)

MMP16, Polyclonal Antibody (Cat# AAA28228)

Full Name
MMP16 Polyclonal Antibody
Gene Names
MMP16; MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
product-image-AAA14911_ELISA.jpg ELISA (ELISA plate was coated with purified human IgG, IgM, and IgA, cynomolgus IgG, and rhesus IgG. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Monkey ads-BIOT (AAA14911) followed by Streptavidin-HRP (please inquire).)

Goat Anti-Human IgG, Monkey ads, Secondary Antibody (Cat# AAA14911)

Full Name
Goat Anti-Human IgG, Monkey ads-BIOT
Reactivity
Human
Applications
ELISA, FLISA
Purity
Affinity chromatography on Human IgG covalently linked to agarose.
Pricing
product-image-AAA26965_IF2.jpg IF (Immunofluorescence) (Immunofluorescent analysis of Hela cells using AAA26965 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

FGFR1, Polyclonal Antibody (Cat# AAA26965)

Full Name
FGFR1 Antibody
Gene Names
FGFR1; CEK; FLG; HH2; OGD; ECCL; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
product-image-AAA26929_WB4.jpg WB (Western Blot) (Immunoprecipitating CA1 in K562 whole cell lysateLane 1: Rabbit control IgG instead of AAA26929 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA26929 (8ug) + K562 whole cell lysate (500ug)Lane 3: K562 whole cell lysate (20ug))

Carbonic anhydrase 1, Polyclonal Antibody (Cat# AAA26929)

Full Name
Rabbit anti-human Carbonic anhydrase 1 polyclonal Antibody
Gene Names
CA1; CAB; CA-I; Car1; HEL-S-11
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
>95%, Protein G purified
Pricing
product-image-AAA13670_FCM4.jpg FCM (Flow Cytometry) (AAA13670 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)

NRF2, Polyclonal Antibody (Cat# AAA13670)

Full Name
Goat anti-NRF2 (aa445-458) Antibody
Gene Names
NFE2L2; NRF2; HEBP1
Reactivity
Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
product-image-AAA14609_IA3.jpg IA (Immuno Assay) (used as capture antibody. Different concentrations were tested (0.5-2 ug/ml).)

Calprotectin, Monoclonal Antibody (Cat# AAA14609)

Full Name
Calprotectin, Human, mAb 27E10, Biotin
Reactivity
Mouse: No; Rhesus Monkey: Yes (subpopulations of macrophages)
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoassay, Immunoprecipitation, Western Blot
Purity
Protein G
Pricing
product-image-AAA28739_FCM2.jpg FCM (Flow Cytometry) (RGS1 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

RGS1, Polyclonal Antibody (Cat# AAA28739)

Full Name
RGS1 Antibody (N-term)
Gene Names
RGS1; 1R20; BL34; IER1; IR20; HEL-S-87
Reactivity
Human
Applications
Western Blot, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA28761_FCM3.jpg FCM (Flow Cytometry) (ENOB Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

ENOB, Polyclonal Antibody (Cat# AAA28761)

Full Name
ENOB Antibody (Center)
Gene Names
ENO3; MSE; GSD13
Reactivity
Human, Mouse, Rat (Predicted Reactivity: Bovine, Pig)
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Pricing
product-image-AAA27673_IP7.png IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27673)

Full Name
VPS35 Antibody, Clone 5A9
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
product-image-AAA30980_WB11.jpg WB (Western Blot) (Western blot analysis of c-Jun phosphorylation expression in UV treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

c-Jun, Polyclonal Antibody (Cat# AAA30980)

Full Name
Phospho-c-Jun (Ser63) Antibody
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA14574_SDS_PAGE11.jpg SDS-PAGE (Figure 11. Non-reduced and reduced SDS-PAGE of showing its purity.)

vimentin, Monoclonal Recombinant Antibody (Cat# AAA14574)

Full Name
Recombinant mouse anti vimentin
Reactivity
Human, mouse
Applications
Immunocytochemistry, Immunohistochemistry, Immunoblot
Purity
The purity of the antibody is >98% as determined by SDS-Polyacrylamide gel electrophoresis.
Pricing
product-image-AAA27679_IP7.png IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27679)

Full Name
VPS35 Antibody, Clone 5A9: HRP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
product-image-AAA28799_FCM3.jpg FCM (Flow Cytometry) (SLC5A12 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

SLC5A12, Polyclonal Antibody (Cat# AAA28799)

Full Name
SLC5A12 Antibody (C-term)
Gene Names
SLC5A12; SMCT2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Pricing
product-image-AAA30988_WB6.jpg WB (Western Blot) (Western blot analysis of Phospho-Tyrosine Hydroxylase (Ser19) expression in various lysates)

Tyrosine Hydroxylase, Polyclonal Antibody (Cat# AAA30988)

Full Name
Phospho-Tyrosine Hydroxylase (Ser19) Antibody
Gene Names
TH; TYH; DYT14; DYT5b
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA28779_WB6.jpg WB (Western Blot) (All lanes : Anti-hDCAMKL1-V705 at 1:8000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lane 3: Rat brain lysate Lane 4: TT whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST)

DCAMKL1, Polyclonal Antibody (Cat# AAA28779)

Full Name
DCAMKL1 Antibody (C-term)
Gene Names
DCLK1; CL1; DCLK; CLICK1; DCDC3A; DCAMKL1
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA27665_IP7.png IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27665)

Full Name
VPS35 Antibody, Clone 7E4: ATTO 390
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
product-image-AAA27666_IP7.png IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27666)

Full Name
VPS35 Antibody, Clone 7E4: ATTO 488
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
product-image-AAA27667_IP7.png IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27667)

Full Name
VPS35 Antibody, Clone 7E4: ATTO 594
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
product-image-AAA27668_IP7.png IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27668)

Full Name
VPS35 Antibody, Clone 7E4: Biotin
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
product-image-AAA27684_IP7.png IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27684)

Full Name
VPS35 Antibody, Clone 8A3: ATTO 488
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
product-image-AAA28712_IF7.jpg IF (Immunofluorescence) (LGR5/GPR49 Antibody (loop2) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).)

LGR5/GPR49, Polyclonal Antibody (Cat# AAA28712)

Full Name
LGR5/GPR49 Antibody (loop2)
Gene Names
LGR5; FEX; HG38; GPR49; GPR67; GRP49
Reactivity
Human, mouse, rat
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA30997_WB11.jpg WB (Western Blot) (Western blot analysis of Phospho-Tau (Ser235) expression in various lysates)

Tau, Polyclonal Antibody (Cat# AAA30997)

Full Name
Phospho-Tau (Ser235) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA28748_FCM5.jpg FCM (Flow Cytometry) (KRAS Antibody (C-term)(AAA28748) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

KRAS, Polyclonal Antibody (Cat# AAA28748)

Full Name
KRAS Antibody (C-term)
Gene Names
KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
Reactivity
Human, Mouse, Rat (Predicted: Rat)
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
product-image-AAA11670_IHC7.jpg IHC (Immunohistochemistry) (Figure 7. IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (AAA11670).Lipocalin 2 was detected in frozen section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Lipocalin 2 Antibody (AAA11670) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Lipocalin 2, Polyclonal Antibody (Cat# AAA11670)

Full Name
Anti-Lipocalin 2 Antibody
Gene Names
Lcn2; 24p3; Sip24; AW212229
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
product-image-AAA23914_APP6.jpg Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/1904) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Estrogen Receptor, alpha, Monoclonal Antibody (Cat# AAA23914)

Full Name
Estrogen Receptor, alpha (Marker of Estrogen Dependence)
Gene Names
ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
product-image-AAA30930_IHC9.jpg IHC (Immunohistchemistry) (Phospho-ERK1/2 (Thr202/Tyr204) Antibody for IHC in human testis)

ERK1/2, Polyclonal Antibody (Cat# AAA30930)

Full Name
Phospho-ERK1/2 (Thr202/Tyr204) Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA11648_IF7.jpg IF (Immunofluorescence) (IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-Band 3 Antibody overnight at 4 degree C. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

Band 3, Polyclonal Antibody (Cat# AAA11648)

Full Name
Anti-Band 3 Antibody
Gene Names
SLC4A1; DI; FR; SW; WD; WR; AE1; CHC; SAO; WD1; BND3; EPB3; SPH4; CD233; EMPB3; RTA1A
Reactivity
Human, Mouse , Rat.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing

Rabbit anti Monkey IgG Fab, Polyclonal Secondary Antibody (Cat# AAA14570)

Full Name
Rabbit anti Monkey IgG Fab
Reactivity
Monkey
Applications
Immunofluorescence, Dot Blot, Immunoblot
Purity
Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Pricing
product-image-AAA30946_IHC8.jpg IHC (Immunohistochemistry) (AAA30946 at 1/100 staining rat cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Chk1, Polyclonal Antibody (Cat# AAA30946)

Full Name
Phospho-Chk1 (Ser286) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA28762_IHC4.jpg IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human testis tissue reacted with ACE2 (SARS Receptor) Antibody (C-term) (AAA28762), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

ACE2 (SARS Receptor), Polyclonal Antibody (Cat# AAA28762)

Full Name
ACE2 (SARS Receptor) Antibody (C-term)
Gene Names
ACE2; ACEH
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA14902_APP.jpg Application Data (Human peripheral blood lymphocytes were stained with Goat F(ab’)2 Anti-Human IgD-AF488 and Mouse Anti-Human CD19-PE.)

Goat F(ab')2 Anti-Human IgD (delta chain specific), Polyclonal Secondary Antibody (Cat# AAA14902)

Full Name
Goat F(ab')2 Anti-Human IgD-UNLB
Reactivity
Human
Applications
Flow Cytometry
Purity
Gel filtration chromatography of pepsin digested antibody.
Pricing
product-image-AAA28222_WB.jpg WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SLC34A2 antibody (AAA28222) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

SLC34A2, Polyclonal Antibody (Cat# AAA28222)

Full Name
SLC34A2 Polyclonal Antibody
Gene Names
SLC34A2; NPTIIb; NAPI-3B; NAPI-IIb
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Pricing
product-image-AAA30965_IHC13.jpg IHC (Immunohistochemistry) (AAA30965 at 1/100 staining mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Myc, Polyclonal Antibody (Cat# AAA30965)

Full Name
Phospho-Myc (Ser62) Antibody
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA28220_IF7.jpg IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using FRMD6 antibody (AAA28220) at dilution of 1:100. Blue: DAPI for nuclear staining.)

FRMD6, Polyclonal Antibody (Cat# AAA28220)

Full Name
FRMD6 Polyclonal Antibody
Gene Names
FRMD6; EX1; Willin; C14orf31; c14_5320
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
product-image-AAA26912_IP6.jpg IP (Immunoprecipitation) (Immunoprecipitating GPT in Mouse liver tissueLane 1: Rabbit control IgG instead of AAA26912 U in Mouse liver tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: AAA26912 (8?g) + Mouse liver tissue (500?g)Lane 3: Mouse liver tissue (10?g))

Alanine aminotransferase 1, Polyclonal Antibody (Cat# AAA26912)

Full Name
Rabbit anti-human Alanine aminotransferase 1 polyclonal Antibody
Gene Names
GPT; AAT1; ALT1; GPT1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
>95%, Protein G purified
Pricing

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