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Reactivity
Gene Name
Isotype
Host
Application
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2671 results for " Lysates" - showing 2200-2250
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of THP-1 cells using CD11a/LFA-1A/ITGAL Rabbit pAb (AAA10672) at dilution of 1:200. Blue: DAPI for nuclear staining.)
ITGAL, Polyclonal Antibody (Cat# AAA10672)
Full Name
ITGAL Polyclonal Antibody
Gene Names
ITGAL; CD11A; LFA-1; LFA1A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human stomach using TFF1 antibody at dilution of 1:200 (40x lens).)
TFF1, Polyclonal Antibody (Cat# AAA10666)
Full Name
TFF1 Polyclonal Antibody
Gene Names
TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using GNA15 antibody (AAA10668) at dilution of 1:100 (40x lens).)
GNA15, Polyclonal Antibody (Cat# AAA10668)
Full Name
GNA15 Polyclonal Antibody
Gene Names
GNA15; GNA16
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat testis using PIBF1 Antibody at dilution of 1:200 (40x lens).)
PIBF1, Antibody (Cat# AAA10724)
Full Name
PIBF1 Polyclonal Antibody
Gene Names
PIBF1; PIBF; CEP90; C13orf24
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(AAA31005 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Tau, Polyclonal Antibody (Cat# AAA31005)
Full Name
Phospho-Tau (Ser262) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
p38 gamma/delta, Polyclonal Antibody (Cat# AAA31407)
Full Name
Phospho-p38 gamma/delta (Tyr185/Tyr182) Antibody
Gene Names
MAPK12; ERK3; ERK6; SAPK3; PRKM12; SAPK-3; P38GAMMA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immuofluorescence)
(Figure 9 Immunofluorescence Validation of ACE2 In Caco2 Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with AAA10928 at 20 ug/mL,followed by goat anti-rabbit IgG secondary antibody at1/500 dilution (green) and DAPI staining (blue). Imageshowing membrane staining on Caco2 cells.)
ACE2, Polyclonal Antibody (Cat# AAA10928)
Full Name
ACE2 Antibody
Gene Names
ACE2; ACEH
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
ACE2 Antibody is affinity chromatography purified via peptide column.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Cleaved-Caspase 1, Polyclonal Antibody (Cat# AAA31336)
Full Name
Cleaved-Caspase 1 (Ala317), p10 Antibody
Gene Names
CASP1; ICE; P45; IL1BC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
WB (Western Blot)
(Western blot analysis of MLZE expression in mouse spleen (A), rat kidney (B), rat spleen (C) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 58 kD))
MLZE, Polyclonal Antibody (Cat# AAA27810)
Full Name
Anti-MLZE Antibody
Gene Names
GSDMC; MLZE
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using MT2A antibody at dilution of 1:200 (40x lens).)
MT2A, Polyclonal Antibody (Cat# AAA10681)
Full Name
MT2A Polyclonal Antibody
Gene Names
MT2A; MT2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
WB (Western Blot)
(PVRL4 Antibody (Center) (AAA28678) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PVRL4 antibody detected the PVRL4 protein (arrow).)
PVRL4, Polyclonal Antibody (Cat# AAA28678)
Full Name
PVRL4 Antibody (Center)
Gene Names
PVRL4; LNIR; PRR4; EDSS1; nectin-4
Reactivity
Human
Applications
Western Blot, Dot Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using BRCA2 antibody (AAA10700) at dilution of 1:100. Blue: DAPI for nuclear staining.)
BRCA2, Polyclonal Antibody (Cat# AAA10700)
Full Name
BRCA2
Gene Names
BRCA2; FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2
Reactivity
Human , Rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using TYR antibody (AAA10704) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
TYR, Polyclonal Antibody (Cat# AAA10704)
Full Name
TYR Polyclonal Antibody
Gene Names
TYR; ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(AAA31063 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
NF kappaB p65, Polyclonal Antibody (Cat# AAA31063)
Full Name
Phospho-NF kappaB p65 (Ser281) Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded Rat testis using LETMD1 Rabbit pAb (AAA10722) at dilution of 1:100 (40x lens).)
LETMD1, Polyclonal Antibody (Cat# AAA10722)
Full Name
LETMD1 Polyclonal Antibody
Gene Names
LETMD1; HCCR1; HCCR-1; HCCR-2; HCRR-2; 1110019O13Rik
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(DDR1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
DDR1, Polyclonal Antibody (Cat# AAA28772)
Full Name
DDR1 Antibody (Center)
Gene Names
DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with TGF-beta (100ng/ml, 30 min), using Phospho-SMAD4 Antibody(upper) or Beta-actin (lower).)
Phospho-SMAD4 (T277), Polyclonal Antibody (Cat# AAA28715)
Full Name
Phospho-SMAD4 (T277) Antibody
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human, Mouse
Predicted Species Reactivity: Rat
Predicted Species Reactivity: Rat
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-FAK (Tyr576) Antibody expression in NIH-3T3 cells lysates.The lane on the right is treated with the antigen-specific peptide.)
FAK, Polyclonal Antibody (Cat# AAA31066)
Full Name
Phospho-FAK (Tyr576) Antibody
Gene Names
PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Paraffin embedded sections of human urothelium were incubated with anti-human Hsp90 (1:100)for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detectedusing Diaminobenzidine (DAB))
Hsp90, Monoclonal Antibody (Cat# AAA11726)
Full Name
Hsp90 antibody
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
Reactivity
Human,
Applications
Western Blot, Immunohistochemistry
Purity
By protein-G affinity chromatography
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
FAK, Polyclonal Antibody (Cat# AAA31446)
Full Name
Phospho-FAK (Ser722) Antibody
Gene Names
PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cells using AHR Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining)
AHR, Polyclonal Antibody (Cat# AAA10754)
Full Name
AHR Polyclonal Antibody
Gene Names
AHR; bHLHe76
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity Purification
Pricing
WB (Western Blot)
(The cell lysates(10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NMNAT-1 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: NMNAT-1 Transfected 293T cell lysate)
NMNAT-1, Monoclonal Antibody (Cat# AAA11734)
Full Name
NMNAT-1
Gene Names
NMNAT1; LCA9; NMNAT; PNAT1
Reactivity
Human
Applications
Western Blot
Purity
Protein-A affinity chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cellsusing Fatty Acid Synthase antibody(AAA10626). Blue: DAPI for nuclear staining)
Fatty Acid Synthase, Polyclonal Antibody (Cat# AAA10626)
Full Name
FASN Polyclonal Antibody
Gene Names
FASN; FAS; OA-519; SDR27X1
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
Purity
Affinity purification
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
alpha Tubulin, Polyclonal Antibody (Cat# AAA31344)
Full Name
Acetyl-alpha Tubulin (Lys40) Antibody
Gene Names
TUBA1B; K-ALPHA-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(All lanes: Anti-Zebrafish wnt8a Antibody (Center) at 1:1000-1:2000 dilutionLane 1: zebrafish muscle lysateLane 2: ZF4 whole cell lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 40 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
wnt8a, Polyclonal Antibody (Cat# AAA28796)
Full Name
Zebrafish wnt8a Antibody (Center)
Gene Names
wnt8a; wnt8; wnt-8; wnt8.1; wu:fa20e02; wu:fe05d07
Reactivity
Zebrafish
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse stomach using ACVR1C antibody at dilution of 1:100 (40x lens).)
ACVR1C, Polyclonal Antibody (Cat# AAA10695)
Full Name
ACVR1C Polyclonal Antibody
Gene Names
ACVR1C; ALK7; ACVRLK7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using DiMethyl-Histone H3-K4 Rabbit pAb (AAA10651) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
H3K4me2, Polyclonal Antibody (Cat# AAA10651)
Full Name
Histone H3K4me2 Polyclonal Antibody
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(TSPAN1 Antibody (Center) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
TSPAN1, Polyclonal Antibody (Cat# AAA28745)
Full Name
TSPAN1 Antibody (Center)
Gene Names
TSPAN1; NET1; TM4C; TM4SF
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
((0.3ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
GPX4, Polyclonal Antibody (Cat# AAA13650)
Full Name
Goat anti-GPX4 (Isoform a and c) Antibody
Gene Names
GPX4; MCSP; SMDS; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
Reactivity
Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow
Applications
Peptide ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with TGF-Beta 1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
TGF-Beta 1, Polyclonal Antibody (Cat# AAA29953)
Full Name
TGF-Beta 1 Antibody
Gene Names
TGFB1; CED; LAP; DPD1; TGFB; TGFbeta
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Peptide affinity purified
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using C-CBL mouse mAb with DAB staining.)
c-Cbl, Monoclonal Antibody (Cat# AAA14105)
Full Name
Anti-c-Cbl
Gene Names
CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry
Purity
Ascitic Fluid
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-p73 (Tyr99) expression in Mouse lung lysate)
p73, Polyclonal Antibody (Cat# AAA30950)
Full Name
Phospho-p73 (Tyr99) Antibody
Gene Names
TP73; P73
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31008 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Bcr, Polyclonal Antibody (Cat# AAA31008)
Full Name
Phospho-Bcr (Tyr360) Antibody
Gene Names
BCR; ALL; CML; PHL; BCR1; D22S11; D22S662
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of Cyclin D1 Antibody (S90) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
Cyclin D1, Polyclonal Antibody (Cat# AAA28781)
Full Name
Cyclin D1 Antibody (S90)
Gene Names
CCND1; BCL1; PRAD1; U21B31; D11S287E
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from Hela and MCF cells using NRF2 Mouse mAb at 1:1000 dilution. Predicted band size: 68KDa. Observed band size: 68kDa.)
NRF2, Monoclonal Antibody (Cat# AAA14162)
Full Name
Anti-NRF2 Mouse mAb
Gene Names
NFE2L2; NRF2; HEBP1
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Pricing
ICC (Immunocytochemistry)
(ICC staining U1A in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
U1A, Monoclonal Antibody (Cat# AAA30476)
Full Name
U1A Antibody
Gene Names
SNRPA; U1A; Mud1; U1-A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
IF (Immunofluorescence)
(AAA31472 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (#AAA31472) and rabbit anti-beta tubulin Ab (#) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-mouse IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)
Claudin 18.2, Monoclonal Antibody (Cat# AAA31472)
Full Name
Claudin 18.2 Mouse monoclonal antibody
Gene Names
CLDN18; SFTA5; SFTPJ; DKFZp564B2062
Reactivity
Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Affinity-chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of FAP alpha staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
FAP alpha, Polyclonal Antibody (Cat# AAA27804)
Full Name
Anti-FAP alpha Antibody
Gene Names
FAP; FAPA; SIMP; DPPIV
Reactivity
Human, Mouse, Rat, Bovine
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
FCM (Flow Cytometry)
(ENOA Antibody (N-term) (Catalog Number: AAA28805) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
ENOA, Polyclonal Antibody (Cat# AAA28805)
Full Name
ENOA Antibody (N-term)
Gene Names
ENO1; NNE; PPH; MPB1; ENO1L1; HEL-S-17
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Monkey
Predicted: Bovine, Monkey
Applications
Immunofluorescence, Western Blot, Flow Cytometry
Purity
Purified Rabbit Polyclnal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Figure 8 Immunofluorescence Validation of JMJD3 in K562 Cells Immunofluorescent analysis of 4% paraformaldehyde fixed K562 cells labeling JMJD3 with AAA10925 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).)
JMJD3, Antibody (Cat# AAA10925)
Full Name
JMJD3 Antibody
Gene Names
KDM6B; JMJD3
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
JMJD3 Antibody is affinity chromatography purified via peptide column.
Pricing
Application Data
(Immunofluorescence staining of Autophagy SQSTM1 (p62) Antibody (C-term) (AAA28682) on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.)
SQSTM1 (p62), Polyclonal Antibody (Cat# AAA28682)
Full Name
SQSTM1 (p62) Antibody (C-term)
Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B; FTDALS3
Reactivity
Human,Mouse,Rat
Predicted: Rat
Predicted: Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of CD122 staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
CD122, Polyclonal Antibody (Cat# AAA27806)
Full Name
Anti-CD122 Antibody
Gene Names
IL2RB; CD122; IL15RB; P70-75
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of APP/Protease Nexin II in U87MG cells. The cell was stained with AAA11729 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
APP, Monoclonal Antibody (Cat# AAA11729)
Full Name
APP antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence
Purity
By protein-G affinity chromatography
Pricing
IHC (Immunohistochemistry)
(AAA30960 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk2, Polyclonal Antibody (Cat# AAA30960)
Full Name
Phospho-Chk2 (Thr68) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(PE conjugatedMouse anti Human CD169 antibody, clone 7-239 used to block CD169 function on myeloid cells.Image caption:Siglec-1 mediates HIV-1 uptake into a storage compartment and enhances HIV-1 trans-infection specially in IFN?-treated monocytes and DCs. A. Uptake of HIV-1NL4–3 by different myeloid cells exposed to IFN?. Cells were cultured with HIV-1 to measure p24Gag by ELISA. Mean values and SEM from four experiments include cells from 12 donors. B. Fold change in HIV-1NL4–3 uptake of cells treated with bafilomycin A1 compared to untreated cells. Mean values and SEM include cells from three donors. C. Relative uptake of HIV-1NL4–3 by IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 uptake by mock-treated cells (set at 100%). Mean values and SEM from two experiments include cells from six donors. D. Confocal microscopy analysis of different IFN?-treated myeloid cells pulsed with HIV-1Cherry and stained for Siglec-1 (Alexa 488), HLA-DR (Alexa 647) and DAPI. (Top) Representative viral pattern for each kind of myeloid cell analyzed, showing maximum fluorescence intensity of four channels. (Bottom) Percentage of myeloid cells with distinct viral patterns: random distribution, polarized accumulation, and sac-like compartment formation, as illustrated in the left drawing. Mean values of 50 cells from two different donors are shown. E. HIV-1 transmission from IFN?-treated myeloid cells to a luciferase reporter CD4+ cell line. HIV-1 infection was determined by induced luciferase activity in relative light units (RLUs). Mean values and SEM from four experiments include cells from 12 donors. F. Relative HIV-1 transmission from IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 trans-infected by mock-treated cells. Mean values and SEM from two experiments include cells from six donors. Statistical differences were assessed with a paired t test in A and E, and with a one sample t-test in B, C and F.From: Pino M, Erkizia I, Benet S, Erikson E, Fernández-Figueras MT, Guerrero D, Dalmau J, Ouchi D, Rausell A, Ciuffi A, Keppler OT, Telenti A, Kräusslich HG, Martinez-Picado J, Izquierdo-Useros N.HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells.Retrovirology. 2015 May 7;12:37.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
CD169, Monoclonal Antibody (Cat# AAA12264)
Full Name
Mouse Anti Human CD169
Gene Names
SIGLEC1; SN; CD169; SIGLEC-1
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Purity
>95% by SDS PAGE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
Standard Curve (Sample)
BDNF, ELISA Kit (Cat# AAA13518)
Full Name
BDNF Mouse ELISA Kit
Reactivity
Mouse
Applications
ELISA
Pricing
IF (Immunofluorescence)
(AAA31033 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CDC25B, Polyclonal Antibody (Cat# AAA31033)
Full Name
Phospho-CDC25B (Ser323) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human lymph reacted with CD19 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
CD19, Polyclonal Antibody (Cat# AAA28685)
Full Name
CD19 Antibody (N-term)
Gene Names
CD19; B4; CVID3
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunoflouorescence)
(AAA31011 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
ATF2, Polyclonal Antibody (Cat# AAA31011)
Full Name
Phospho-ATF2 (Thr69/51) Antibody
Gene Names
ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(Western blot analysis of CD107b on J774 cell lysate probed with Rat anti Mouse CD107b at 1/500, 1/1000, 1/2000. Rat anti tubulin alpha is included as a loading control. Detection is with Goat anti Rat IgG:Dylight®)
TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12010)
Full Name
RAT ANTI TUBULIN ALPHA
Applications
Immunohistochemistry, Immunofluorescence, Radioimmunoassay, Western Blot
Pricing