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Gene Name
Isotype
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Application
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2304 results for "Secondary Antibody" - showing 2000-2050
IF (Immunofluorescence)
(AAA31114 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31114) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)
ARX, Polyclonal Antibody (Cat# AAA31114)
Full Name
ARX Antibody
Gene Names
ARX; ISSX; PRTS; CT121; EIEE1; MRX29; MRX32; MRX33; MRX36; MRX38; MRX43; MRX54; MRX76; MRX87; MRXS1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific)
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with SF2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
SF2, Monoclonal Antibody (Cat# AAA30465)
Full Name
SF2 Antibody
Gene Names
SRSF1; ASF; SF2; SFRS1; SF2p33; SRp30a
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31174 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
SA1, Polyclonal Antibody (Cat# AAA31174)
Full Name
SA1 Antibody
Gene Names
STAG1; SA1; MRD47; SCC3A
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(AAA31129 at 1/100 staining Rat ovary tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IL10, Polyclonal Antibody (Cat# AAA31129)
Full Name
IL10 Antibody
Gene Names
IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31255 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
SAP155, Polyclonal Antibody (Cat# AAA31255)
Full Name
SAP155 Antibody
Gene Names
SF3B1; MDS; PRP10; Hsh155; PRPF10; SAP155; SF3b155
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(Immunofluorescence (IF) staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31125) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
NR0B1, Polyclonal Antibody (Cat# AAA31125)
Full Name
NR0B1 Antibody
Gene Names
NR0B1; AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
peptide affinity purification
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27697)
Full Name
VPS35 Antibody, Clone 10A8: HRP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27702)
Full Name
VPS35 Antibody, Clone 11H10: ATTO 488
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27700)
Full Name
VPS35 Antibody, Clone 11H10
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
eIF2B epsilon, Polyclonal Antibody (Cat# AAA31414)
Full Name
Phospho-eIF2B epsilon (Ser540) Antibody
Gene Names
EIF2B5; CLE; CACH; LVWM; EIF-2B; EIF2Bepsilon
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (80%), Bovine (80%), Horse (80%), Rabbit (80%), Dog (80%), Chicken (80%)
Predicted Reactivity: Pig (80%), Bovine (80%), Horse (80%), Rabbit (80%), Dog (80%), Chicken (80%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31031 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CDC25A, Polyclonal Antibody (Cat# AAA31031)
Full Name
Phospho-CDC25A (Ser124) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(AAA31087 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
CREB, Polyclonal Antibody (Cat# AAA31087)
Full Name
CREB Antibody
Gene Names
CREB1; CREB; CREB-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IF (Immunofluorescence)
(AAA31185 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31185 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Laminin 2 alpha, Polyclonal Antibody (Cat# AAA31185)
Full Name
Laminin 2 alpha Antibody
Gene Names
LAMA2; LAMM; MDC1A
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin .
Pricing
IF (Immunofluorescence)
(AAA31202 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31202 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
GABA A Receptor alpha 5, Polyclonal Antibody (Cat# AAA31202)
Full Name
GABA A Receptor alpha 5 Antibody
Gene Names
GABRA5; EIEE79
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(88%), Chicken(100%)
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(88%), Chicken(100%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry)
(AAA31064 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
PKC theta, Polyclonal Antibody (Cat# AAA31064)
Full Name
Phospho-PKC theta (Ser676) Antibody
Gene Names
PRKCQ; PRKCT; nPKC-theta
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells cells using AAA15913 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Alpha-enolase, Polyclonal Antibody (Cat# AAA15913)
Full Name
Rabbit anti- human Alpha-enolase polyclonal Antibody
Gene Names
ENO1; NNE; PPH; MPB1; ENO1L1; HEL-S-17
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27687)
Full Name
VPS35 Antibody, Clone 8A3: FITC
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31101at 1/100 staining human mammary cancer tissue byIHC-P. The sample was formaldehyde fixed and a heatmediated antigen retrieval step in citrate buffer wasperformed. The sample was then blocked and incubated withthe antibody for 1.5 hours at 22°C. An HRP conjugated goatanti-rabbit antibody was used as the secondary antibody.)
xCT, Polyclonal Antibody (Cat# AAA31101)
Full Name
xCT Antibody
Gene Names
SLC7A11; xCT; CCBR1
Reactivity
Human, Mouse, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27683)
Full Name
VPS35 Antibody, Clone 8A3: ATTO 390
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IF (Immunofluorescence)
(AAA31270 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31270 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Hyaluronan synthase 2, Polyclonal Antibody (Cat# AAA31270)
Full Name
Hyaluronan synthase 2 Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry
Purity
Peptide affinity purification
Pricing
IHC (Immunohistochemistry)
(Figure 3. IHC analysis of SFTPA1 using anti- SFTPA1 antibody (AAA11521).SFTPA1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- SFTPA1 Antibody (AAA11521) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Surfactant Protein A, Polyclonal Antibody (Cat# AAA11521)
Full Name
Polyclonal Anti-SFTPA1 Antibody
Gene Names
Sftpa1; SP-A; Sftp1; Sftp-1
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IHC-P (Immunohistochemistry Paraffin)
(AAA31041 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was use das the secondary antibody.)
STAT3, Polyclonal Antibody (Cat# AAA31041)
Full Name
Phospho-STAT3 (Tyr705) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Peptide ELISA
Purity
Peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
HRI, Polyclonal Antibody (Cat# AAA28727)
Full Name
HRI Antibody (N-term)
Gene Names
EIF2AK1; HCR; HRI
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western blot analysis of AFX phosphorylation expression in serum treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
AFX, Polyclonal Antibody (Cat# AAA31062)
Full Name
Phospho-AFX (Ser197) Antibody
Gene Names
FOXO4; AFX; AFX1; MLLT7
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
CSK, Polyclonal Antibody (Cat# AAA31403)
Full Name
Phospho-CSK (Tyr304) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating TUBB in Hela whole cell lysateLane 1: Mouse control IgG instead in Hela whole cell lysate.Lane 2: (2ug) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (5ug)For western blotting, the blot was detected at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:5000)
TUBB, Monoclonal Antibody (Cat# AAA28058)
Full Name
TUBB Monoclonal Antibody
Reactivity
Human, Rat, Rabbit, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein A purified
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Vimentin, Polyclonal Antibody (Cat# AAA31419)
Full Name
Phospho-Vimentin (Ser39) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12211)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
WB (Western Blot)
(Western blotAll lanes: Ribulose-1,5-bisphosphate carboxylase oxygenase antibody at 5ug/mlLane 1: Ribulose-1,5-bisphosphate carboxylase oxygenase at 0.01ug/mlLane 2: Ribulose-1,5-bisphosphate carboxylase oxygenase at 0.1ug/mlLane 3: Ribulose-1,5-bisphosphate carboxylase oxygenase at 1ug/mlLane 4: Ribulose-1,5-bisphosphate carboxylase oxygenase at 10ug/mlSecondaryGoat polyclonal to rabbit at 1/15000 dilutionPredicted band size: 55kDa, 14kDaObserved band size: 55kDa, 14kDa)
RuBisCO, Polyclonal Antibody (Cat# AAA15912)
Full Name
Rabbit anti-RuBisCO polyclonal Antibody
Reactivity
Spinach
Applications
Western Blot
Purity
>95%, Protein G purified
Pricing
Standard Curve (Sample)
Mouse Anti-Human IgG (Fc) (gamma chain specific), Monoclonal Secondary Antibody (Cat# AAA14898)
Full Name
Mouse Anti-Human IgG (Fc)-APC
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Pricing
IHC (Immunohistchemistry)
(AAA30947 at 1/100 staining rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk1, Polyclonal Antibody (Cat# AAA30947)
Full Name
Phospho-Chk1 (Ser301) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Western blot analysis of lysates from HUVEC and COLO205 cells. The lane on the right is blocked with the synthesized peptide. Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)
FGR, Polyclonal Antibody (Cat# AAA29727)
Full Name
FGR Antibody
Gene Names
FGR; SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antigen Affinity Purification
Pricing
IF (Immunofluorescence)
(AAA30937 staining A-431 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
SYK, Polyclonal Antibody (Cat# AAA30937)
Full Name
Phospho-SYK (Tyr323) Antibody
Gene Names
SYK; p72-Syk
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30938 at 1/100 staining human muscle tumor tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
JAK1, Polyclonal Antibody (Cat# AAA30938)
Full Name
Phospho-JAK1 (Tyr1034/Tyr1035) Antibody
Gene Names
JAK1; JTK3; JAK1A; JAK1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Intracellular flow cytometric analysis of RIG-I in K562 Cell line using 0.5 ug/10^6 cells of Anti-RIGI antibody (ABM4H29). Green represent isotype control and red represent Anti-RIG I antibody (AAA14869). Goat anti-mouse PE conjugate was used as secondary.)
RIG I /DDx58, Monoclonal Antibody (Cat# AAA14869)
Full Name
Monoclonal Antibody to RIG-I (Clone: ABM4H29)
Gene Names
DDX58; RIGI; RIG-I; RLR-1; SGMRT2
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
Protein G chromatography
Pricing
IHC (Immunohistochemistry)
(AAA30953 at 1/100 staining rat ovarian tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
MAP3K7, Polyclonal Antibody (Cat# AAA30953)
Full Name
Phospho-MAP3K7 (Thr187) Antibody
Gene Names
MAP3K7; CSCF; FMD2; TAK1; MEKK7; TGF1a
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30945 at 1/100 staining rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk1, Polyclonal Antibody (Cat# AAA30945)
Full Name
Phospho-Chk1 (Ser296) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Flow cytometry analysis of Podoplanin in HeLa cells. The cell was stained with (AAA11728) at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
PDPN, Monoclonal Antibody (Cat# AAA11728)
Full Name
PDPN antibody
Gene Names
PDPN; T1A; GP36; GP40; Gp38; OTS8; T1A2; TI1A; T1A-2; AGGRUS; HT1A-1; PA2.26
Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry, Immunofluorescence
Purity
By protein-G affinity chromatography
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of BCOX1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
BCOX1, Polyclonal Antibody (Cat# AAA27816)
Full Name
Anti-BCOX1 Antibody
Gene Names
KIAA0100; BCOX; BCOX1; CT101
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Immunogen affinity chromatography
Pricing
IF (Immunofluorescence)
(AAA30936 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
NF kappaB p65, Polyclonal Antibody (Cat# AAA30936)
Full Name
Phospho-NF kappaB p65 (Ser536) Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using AAA28679 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
HK1 (Hexokinase), Polyclonal Antibody (Cat# AAA28679)
Full Name
HK1 (Hexokinase) Antibody (N-term)
Gene Names
HK1; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Jurkat cells, using CD3D Antibody.)
CD3D, Monoclonal Antibody (Cat# AAA30236)
Full Name
CD3D Antibody
Gene Names
CD3D; T3D; IMD19; CD3-DELTA
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
Affinity - chromatography.
Pricing
IHC (Immunohistochemistry)
(Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.)
ITGAM/CD11B, Polyclonal Antibody (Cat# AAA13654)
Full Name
Goat anti-ITGAM/CD11B Antibody
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Tested: Human
Expected from sequence similarity: Human
Expected from sequence similarity: Human
Applications
Peptide ELISA, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
(Western Blot using anti-polysialic acid antibody Rat brain lysate was resolved on a 10% SDS PAGE gel and blots probed with at 2 µg/ml before being detected by a secondary antibody.)
Polysialic acid, Recombinant Antibody (Cat# AAA14183)
Full Name
Anti-Polysialic acid [735]
Reactivity
Human, Mouse, Rat
Applications
Immunoblot, Immunoprecipitation, Western Blot, Flow Cytometry, Immunohistochemistry
Purity
Protein A Affinity Purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with p70 S6 Kinase beta antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
p70S6 Kinase beta, Polyclonal Antibody (Cat# AAA29952)
Full Name
p70 S6 Kinase beta Antibody
Gene Names
RPS6KB2; KLS; SRK; S6K2; STK14B; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
Peptide affinity purified
Pricing
WB (Western Blot)
(Figure-6: Western blot analysis of Caspase-8. Anti-Caspase-8 antibody (Clone: ABM14C1) was used at 4 ug/ml on EL-4 lysate.)
Caspase-8, Monoclonal Antibody (Cat# AAA14877)
Full Name
Monoclonal Antibody to Caspase-8 (Clone: ABM14C1)
Gene Names
CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry
Purity
Purified
Protein G Chromatography
Protein G Chromatography
Pricing
IHC (Immunohistochemistry)
(Immunofluorescence staining of HepG2 cells with AAA27007 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
ZDHHC14, Polyclonal Antibody (Cat# AAA27007)
Full Name
ZDHHC14 Antibody
Gene Names
ZDHHC14; NEW1CP
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
IHC (Immunohistchemistry)
(AAA31065 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Dynamin-1, Polyclonal Antibody (Cat# AAA31065)
Full Name
Phospho-Dynamin-1 (Ser774) Antibody
Gene Names
DNM1; DNM; EIEE31
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western Blot analysis for galactocerebroside in PC12 lysate. PC12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with AAA14685 at 1:500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein Galactocerebroside (~75kD).)
Galactocerebroside, Monoclonal Antibody (Cat# AAA14685)
Full Name
Mouse anti-Bovine Galactocerebroside
Applications
Immunohistochemistry, Immunocytochemistry
Purity
Purified by Protein A affinity chromatography.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with CD86 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
CD86, Monoclonal Antibody (Cat# AAA30048)
Full Name
CD86 Antibody
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing