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Type
Reactivity
Gene Name
Isotype
Host
Application
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1507 results for "B cell" - showing 150-200
FCM (Flow Cytometry)
(Flow cytometric analysis of A549 cells with AKT1/2/3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
AKT1/2/3, Monoclonal Antibody (Cat# AAA30101)
Full Name
AKT1/2/3 Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
SDS-PAGE
(Purified ER-beta Mouse Monoclonal Antibody (ESR2/686). Confirmation of Integrity and Purity of Antibody.)
ER-beta1 (Estrogen Receptor beta-1), Monoclonal Antibody (Cat# AAA13846)
Full Name
ER-beta1 (Estrogen Receptor beta-1) Mouse Monoclonal Antibody
Gene Names
ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(AAA31084 at 1/200 staining human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
HSP90B, Polyclonal Antibody (Cat# AAA31084)
Full Name
HSP90B Antibody
Gene Names
HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
Application Data
(Publised customer image:Mouse anti Human CD163 antibody, clone EDHu-1 used for the identification of perivascular macrophages in human brain by immunofluorescence.Image caption:Images demonstrating immunohistological stainings of amylin and double immunofluorescence staining against NG2/amylin, laminin/amylin and CD163/amylin in the hippocampus of the patient with AD and T2D.Amylin cell inclusions are indicated with arrows in (a) and shown in a higher magnification in (b). Pericytes with round cell bodies and NG2-positive coverage of the microvessel surface (green in c), without amylin cell inclusions (red in d), displayed round DAPI-positive cell nuclei (blue in e). The images in (c), (d) and (e) are merged in (f). Cells with more diffuse and weak NG2 staining (indicated by the arrowhead, green in g) and cytosolic amylin cell inclusions (red in h) showed altered cell nuclei (indicated with arrow, blue in i).The adjacent unaffected NG2-positive cell is indicated with an arrowhead in (i). The images in (g), (h) and (i) are merged in (j). Cells enclosed by laminin (green in K) contained amylin grains (red in l) and fragmented DAPI-positive cell nuclei (indicated with an arrow blue in m). The images in (k), (l) and (m) are merged in (n).Loss of NG2 coverage (green in o) was associated with polarized amylin cell inclusion (red in p) and fragmented DAPI-positive cell nuclei (indicated with an arrow, blue in q). The images in (o), (p) and (q) are merged in (r). Staining against macrophage marker CD163 (green in s) did not co-localize with amylin cell inclusions (red in t). The cell nucleus was stained with DAPI (blue in U). The images in (s), (t) and (u) are merged in (v). Scale bars: (a) 50 mum, (b), (c) to (v) 5 mum.From: Schultz, N. et al. (2016).Amylin alters human brain pericyte viability and NG2 expression.J Cereb Blood Flow & Metab. Jun 28 [Epub ahead of print]This is from an open access article distributed under the terms of the Creative Commons Attribution License.)
CD163, Monoclonal Antibody (Cat# AAA12256)
Full Name
Mouse Anti Human CD163: RPE
Gene Names
CD163; M130; MM130; SCARI1
Reactivity
Human
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12182)
Full Name
RAT ANTI MOUSE CD11b:FITC
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human kidney cancer using NFKB2 Antibody at dilution of 1:100 (40x lens).)
NFKB2, Polyclonal Antibody (Cat# AAA10749)
Full Name
NFKB2 Polyclonal Antibody
Gene Names
NFKB2; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot)
(BATF monoclonal antibody Western Blot analysis of BATF expression in Hela NE.)
BATF, Monoclonal Antibody (Cat# AAA24147)
Full Name
BATF (Basic Leucine Zipper Transcriptional Factor ATF-like, B Cell-activating Transcription Factor, SF-HT-activated Gene 2 Protein) (AP)
Gene Names
BATF; SFA2; B-ATF; BATF1; SFA-2
Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12173)
Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human brain section using Pink 1 (AAA28759). AAA28759 was diluted at 1:1000 dilution. A undiluted binotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining)
S100B, Polyclonal Antibody (Cat# AAA28759)
Full Name
S100B Antibody
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human, Mouse, Rat (Predicted: Rabbit)
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of A431 cells using anti-EGFR antibody (AAA19214).Overlay histogram showing A431 cells stained with AAA19214 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EGFR Antibody (AAA19214,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
EGFR, Polyclonal Antibody (Cat# AAA19214)
Full Name
Anti-EGFR Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Anti-TRIM28 rabbit polyclonal antibody at 1:500 dilution Lane A: TRIM28 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:89 kDa Observed band size:115 kDa (We are unsure as to the identity of these extra bands.))
KAP1/TRIM28, Polyclonal Antibody (Cat# AAA27715)
Full Name
Anti-KAP1/TRIM28 Antibody, Rabbit Polyclonal
Gene Names
TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
IF (Immunofluorescence)
(Immunofluorescence-Akt2 Polyclonal Antibody)
Akt2, Polyclonal Antibody (Cat# AAA28287)
Full Name
Akt2 Polyclonal Antibody
Gene Names
AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using XRCC4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
XRCC4, Polyclonal Antibody (Cat# AAA10755)
Full Name
XRCC4 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using ESRRA antibody at 1:1000 dilution.)
ESRRA, Polyclonal Antibody (Cat# AAA30567)
Full Name
ESRRA Polyclonal Antibody
Gene Names
ESRRA; ERR1; ERRa; ESRL1; NR3B1; ERRalpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
Application Data
(Detection limit for recombinant GST tagged PGR is ~0.3ng/ml as a capture antibody.)
Progesterone Receptor, Monoclonal Antibody (Cat# AAA24623)
Full Name
Progesterone Receptor (PgR, PR, Nuclear Receptor Subfamily 3 Group C Member 3, NR3C3) APC
Gene Names
PGR; PR; NR3C3
Reactivity
Human, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Detection limit for recombinant GST tagged STIP1 is 0.03 ng/ml as a capture antibody.)
STIP1, Monoclonal Antibody (Cat# AAA26281)
Full Name
STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (Biotin)
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Applications
Immunofluorescence, Western Blot
Purity
Purified
Pricing
IHC (Immunohistochemistry)
(Immunochemical staining of alpha smooth muscle actin in mouse skeletal muscle with rabbit polyclonal antibody at 1:40000 dilution, formalin-fixed paraffin embedded sections.)
ACTA2, Polyclonal Antibody (Cat# AAA27714)
Full Name
Anti-ACTA2 Antibody, Rabbit Polyclonal
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Pricing
Application Data
(Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. High power)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA12055)
Full Name
RAT ANTI MOUSE CD8 ALPHA:RPE
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Applications
Flow Cytometry
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CD22, Polyclonal Antibody (Cat# AAA31418)
Full Name
Phospho-CD22 (Tyr842) Antibody
Gene Names
CD22; SIGLEC2; SIGLEC-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse (83%)
Predicted Reactivity: Horse (83%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA12146)
Full Name
MOUSE ANTI RAT CD11b:FITC
Gene Names
ITGAM; CD11B
Applications
Flow Cytometry
Pricing
ELISA
(Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA at decreasing concentrations.)
TIGIT, Monoclonal Antibody (Cat# AAA11004)
Full Name
TIGIT Antibody [4A10]
Gene Names
TIGIT; VSIG9; VSTM3; WUCAM
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Protein A purified
Pricing
IHC (Immunohistchemistry)
(Formalin-paraffin human Placenta stained with E-Cadherin MAb (CDH1/1525).)
E-Cadherin/CD324, Monoclonal Antibody (Cat# AAA23896)
Full Name
E-Cadherin/CD324 (Intercellular Junction Marker)
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
Reactivity
Human.
Does not react with Mouse and Rat. Others not known.
Does not react with Mouse and Rat. Others not known.
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(Western blot analysis of HLA-B (arrow) using rabbit polyclonal HLA-B Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-B gene.)
HLA-B, Polyclonal Antibody (Cat# AAA28743)
Full Name
HLA-B Antibody (N-term)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A-431 using Cortactin antibody at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.)
ESRRA, Polyclonal Antibody (Cat# AAA28272)
Full Name
ESRRA Polyclonal Antibody
Gene Names
ESRRA; ERR1; ERRa; ESRL1; NR3B1; ERRalpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
Application Data
(Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. High power)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA11885)
Full Name
RAT ANTI MOUSE CD8 ALPHA:FITC
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Applications
Flow Cytometry
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Smad1/9, Polyclonal Antibody (Cat# AAA31453)
Full Name
Phospho-Smad1/9 (Ser463/Ser465) Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Anti-NFkB p100 Picoband antibody, AAA11631-6.JPGIHC(P): Rat Intestine Tissue)
Nuclear factor NF-kappa-B p100 subunit, Polyclonal Antibody (Cat# AAA11631)
Full Name
Anti-NFkB p100/p52 Antibody
Gene Names
NFKB2; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
Application Data
(Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . Medium power)
CD13, Monoclonal Antibody (Cat# AAA12115)
Full Name
RAT ANTI MOUSE CD13:RPE
Gene Names
Anpep; Apn; AP-M; AP-N; Cd13; P150
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of THP-1 cells using anti-NOX2/gp91phox/CYBB antibody (AAA19218).Overlay histogram showing THP-1 cells stained with AAA19218 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOX2/gp91phox/CYBB Antibody (AAA19218, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NOX2/gp91phox/CYBB, Polyclonal Antibody (Cat# AAA19218)
Full Name
Anti-NOX2/gp91phox/CYBB Antibody
Gene Names
CYBB; CGD; NOX2; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western Blot detection against Immunogen (51.96kD).)
FAM3B, Monoclonal Antibody (Cat# AAA25095)
Full Name
FAM3B (Protein FAM3B, Cytokine-like Protein 2-21, Pancreatic-derived Factor, PANDER, UNQ320/PRO365, PRED44, C21orf76, C21orf11, D21M16SJHU19e) (FITC)
Gene Names
FAM3B; 2-21; ORF9; PANDER; PRED44; C21orf11; C21orf76
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12170)
Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12184)
Full Name
RAT ANTI MOUSE CD11b
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
WB (Western Blot)
(NME2 monoclonal antibody (M11), clone 1E4. Western Blot analysis of NME2 expression in PC-12.)
NME2, Monoclonal Antibody (Cat# AAA25974)
Full Name
NME2 (Non-Metastatic Cells 2, Protein (NM23B) Expressed in, MGC111212, NDPK-B, NDPKB, NM23-H2, NM23B, puf) (AP)
Gene Names
NME2; PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2
Applications
Western Blot
Purity
Purified
Pricing
Application Data
(Detection limit for recombinant GST tagged PGR is ~0.3ng/ml as a capture antibody.)
Progesterone Receptor, Monoclonal Antibody (Cat# AAA24327)
Full Name
Progesterone Receptor (PgR, PR, Nuclear Receptor Subfamily 3 Group C Member 3, NR3C3) (AP)
Gene Names
PGR; PR; NR3C3
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
ELISA
(Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA at decreasing concentrations.)
TIGIT, Monoclonal Antibody (Cat# AAA11006)
Full Name
TIGIT Antibody [4A12]
Gene Names
TIGIT; VSIG9; VSTM3; WUCAM
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Protein A purified
Pricing
WB (Western Blot)
(RUNX1 monoclonal antibody Western Blot analysis of RUNX1 expression in Hela NE.)
RUNX1, Monoclonal Antibody (Cat# AAA25236)
Full Name
RUNX1 (Runt-related Transcription Factor 1, Acute Myeloid Leukemia 1 Protein, Core-binding Factor Subunit alpha-2, CBF-alpha-2, Oncogene AML-1, Polyomavirus Enhancer-binding Protein 2 alpha B Subunit, PEA2-alpha B, PEBP2-alpha B, SL3-3 Enhancer Factor 1 a
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Detection limit for recombinant GST tagged RUNX1 is approximately 1ng/ml as a capture antibody.)
RUNX1, Monoclonal Antibody (Cat# AAA26481)
Full Name
RUNX1 (Runt-Related Transcription Factor 1, AML1, AML1-EVI-1, AMLCR1, CBFA2, EVI-1, PEBP2aB) (HRP)
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug AKT1 antibody . Western blot was performed from the immunoprecipitate using AKT1 antibody at a dilition of 1:1000.)
AKT1, Monoclonal Antibody (Cat# AAA28356)
Full Name
AKT1 Rabbit mAb
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA12045)
Full Name
MOUSE ANTI RAT CD11b:RPE
Gene Names
ITGAM; CD11B
Applications
Flow Cytometry
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPA1 Antibody.)
HNRNPA1, Polyclonal Antibody (Cat# AAA29846)
Full Name
HNRNPA1 Polyclonal Antibody
Gene Names
HNRNPA1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using MMP9 Rabbit pAb (AAA10629) at dilution of 1:100.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
MMP9, Polyclonal Antibody (Cat# AAA10629)
Full Name
MMP9 Polyclonal Antibody
Gene Names
MMP9; GELB; CLG4B; MMP-9; MANDP2
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity purification
Pricing
Application Data
(Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD274:RPE)
CD274, Monoclonal Antibody (Cat# AAA26767)
Full Name
CD274 (CD274 Antigen, B7 Homolog 1, B7H1, B7-H1, B7-H, MGC142294, MGC142296, OTTHUMP00000021029, Programmed Cell Death 1 Ligand 1, PDCD1 Ligand 1, PDCD1L1, PDCD1LG1, Programmed Death Ligand 1, PDL1, PD-L1, RGD1566211) (PE)
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Flow Cytometry
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
Application Data
(ICC image of LGR5 antibody stained NCCIT cells. The secondary antibody (green) was goat anti-rabbit IgG (H+L) FITC conjugated.)
LGR5, Polyclonal Antibody (Cat# AAA17985)
Full Name
LGR5 Antibody
Gene Names
LGR5; FEX; HG38; GPR49; GPR67; GRP49
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(BATF monoclonal antibody Western Blot analysis of BATF expression in Hela NE.)
BATF, Monoclonal Antibody (Cat# AAA24442)
Full Name
BATF (Basic Leucine Zipper Transcriptional Factor ATF-like, B Cell-activating Transcription Factor, SF-HT-activated Gene 2 Protein) APC
Gene Names
BATF; SFA2; B-ATF; BATF1; SFA-2
Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western Blot detection against Immunogen (37.84kD).)
HSPB1, Monoclonal Antibody (Cat# AAA25425)
Full Name
HSPB1 (Heat Shock Protein beta-1, HspB1, Heat Shock 27kD Protein, HSP 27, Stress-responsive Protein 27, SRP27, Estrogen-regulated 24kD Protein, 28kD Heat Shock Protein, HSPB1, HSP27) (HRP)
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Biotin)
CD11b, Monoclonal Antibody (Cat# AAA11991)
Full Name
MOUSE ANTI HUMAN CD11b
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Baboon, Cynomolgus monkey, Rhesus Monkey
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA11969)
Full Name
MOUSE ANTI RAT CD11b
Gene Names
ITGAM; CD11B
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
WB (Western Blot)
(Figure 9 KD Validation in HeLa Cells (Horinaka et al., 2005)HeLa cells were transfected with DR4siRNA or LacZ control siRNA. At 24 h after transfection, the cells were treated with or without 20 μM luteolin for 24 h. Western blot analysis was carried out with anti-DR4 antibodies. DR4 expression was markedly reduced after DR4 knockdown.)
DR4, Polyclonal Antibody (Cat# AAA10950)
Full Name
DR4 Antibody
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Reactivity
Human
Applications
Western Blot
Purity
DR4 Antibody is affinity chromatography purified via peptide column.
Pricing
ICC (Immunocytochemistry)
(ICC staining EGFR in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
EGFR, Monoclonal Antibody (Cat# AAA30010)
Full Name
EGFR Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using SIRT1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
SIRT1, Polyclonal Antibody (Cat# AAA28348)
Full Name
[KO Validated] SIRT1 Rabbit pAb
Gene Names
SIRT1; SIR2L1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity purification
Pricing