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183 results for " Conjugated Secondary Antibodies" - showing 150-183
WB (Western Blot)
(Western blot analysis of SLC10A1 using anti- SLC10A1 antibody (AAA11653).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates (positive control), Lane 2: rat kidney tissue lysates, (negative control)After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1 antigen affinity purified polyclonal antibody (AAA11653) at 0.25ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50KD. The expected band size for SLC10A1 is at 38KD.)
SLC10A1, Polyclonal Antibody (Cat# AAA11653)
Full Name
Anti-SLC10A1 Antibody
Gene Names
Slc10a1; Ntcp
Reactivity
Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11864)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
Immunofluorescence
Pricing
WB (Western Blot)
(Western Blot analysis for galactocerebroside in PC12 lysate. PC12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with AAA14685 at 1:500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein Galactocerebroside (~75kD).)
Galactocerebroside, Monoclonal Antibody (Cat# AAA14685)
Full Name
Mouse anti-Bovine Galactocerebroside
Applications
Immunohistochemistry, Immunocytochemistry
Purity
Purified by Protein A affinity chromatography.
Pricing
WB (Western Blot)
(All lanes : Anti-ERVK-7 Antibody (N-Term) at 1:1000-1:2000 dilutionLane 1: Raji whole cell lysateLane 2: human lung lysateLane 3: human testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 67 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
ERVK-7, Polyclonal Antibody (Cat# AAA28797)
Full Name
ERVK-7 Antibody (N-Term)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(Flow Cytometry detection of FOLR1 in MCF-7 human cell line using AAA14691. MCF-7 human breast cancer cell line was stained with AAA14691 (filled histogram) or isotype control antibody (open histogram), followed byPhycoerythrin-conjugated Anti-MouseIgG Secondary Antibody.)
FOLR1, Monoclonal Antibody (Cat# AAA14691)
Full Name
FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)
Gene Names
FOLR1; FBP; FOLR
Reactivity
Human
Applications
Flow Cytometry, Western Blot, Immunocytochemistry
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Pricing
Goat anti Human IgG IgA IgM (Fc specific), Polyclonal Secondary Antibody (Cat# AAA14569)
Full Name
Goat anti Human IgG IgA IgM (Fc specific), conjugated with FITC
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Purity
The IgG (7S) fraction is isolated and purified from hyperimmune antisera with strong precipitating activity and contains the bulk of the antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodi
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term) (Cat.#AAA28756), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
EN1 (Engrailed 1), Polyclonal Antibody (Cat# AAA28756)
Full Name
EN1 (Engrailed 1) Antibody (N-term)
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
anti-Human IgG+HRP, Polyclonal Secondary Antibody (Cat# AAA13852)
Full Name
Goat anti-Human IgG (H+L) +HRP
Reactivity
Human
Applications
Western Blot
Pricing
anti-Mouse IgG+HRP, Secondary Antibody (Cat# AAA13853)
Full Name
anti-Mouse IgG+HRP
Reactivity
Mouse
Applications
Western Blot
Pricing
WB (Western Blot)
(Western blot: primary antibody, anti-envelope (Zika virus) rabbit polyclonal antibody (1:2000 dilution); secondary antibody, HRP-conjugated goat anti-rabbit IgG (1:4000)A, full length envelope protein of Zika virus)
Envelope (E) (Zika Virus), Antibody (Cat# AAA13695)
Full Name
Anti-Envelope (E) (Zika Virus) Polyclonal Antibody, Rabbit Serum
Applications
Western Blot
Pricing
FCM (Flow Cytometry)
(ENAM Antibody (C-term) (AAA28699) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
ENAM, Polyclonal Antibody (Cat# AAA28699)
Full Name
ENAM Antibody (C-term)
Gene Names
ENAM; ADAI; AI1C; AIH2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(AAA28674 staining GLUL in Human liver tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
GLUL, Polyclonal Antibody (Cat# AAA28674)
Full Name
GLUL Antibody (N-term)
Gene Names
GLUL; GS; GLNS; PIG43; PIG59
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Chicken, Monkey, Pig)
Applications
Western Blot, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(Western blot analysis of lysates from 293T cell line human brain and mouse brain tissue lysate(from left to right), using STRADA Antibody (C-term). AAA28673 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
STRADA, Polyclonal Antibody (Cat# AAA28673)
Full Name
STRADA Antibody (C-term)
Gene Names
STRADA; LYK5; PMSE; Stlk; STRAD; NY-BR-96
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Applications
Western Blot, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(RSPO2 Antibody (C-term) (AAA28708) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
RSPO2, Polyclonal Antibody (Cat# AAA28708)
Full Name
RSPO2 Antibody (C-term)
Gene Names
RSPO2; CRISTIN2
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Westen Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11850)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
Immunohistochemistry, Western Blot
Pricing
IgG, Polyclonal Secondary Antibody (Cat# AAA14879)
Full Name
APC conjugated Goat anti Rabbit IgG (H+L)
Applications
Immunofluorescence, Flow Cytometry
Purity
Affinity Chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescence (IFC) analysis of PC3 cells using AAA14697 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Ribonuclease inhibitor 1, Monoclonal Antibody (Cat# AAA14697)
Full Name
Ribonuclease inhibitor 1 (RNH1)
Gene Names
RNH1; RAI; RNH; MGC4569; MGC18200; MGC54054
Applications
Immunofluorescence, Western Blot
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
WB (Western Blot)
(Anti-SLC6A19 Antibody (C-Term) at 1:2000 dilution + 293 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 71 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
SLC6A19, Polyclonal Antibody (Cat# AAA28802)
Full Name
SLC6A19 Antibody (C-Term)
Gene Names
SLC6A19; HND; B0AT1
Reactivity
Human
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data
(Overlay histogram showing U-2OS cells stained with AP10510c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated n 2% bovine serum albumin to block non-specificprotein-protein interactions followed by the antibody (AP10510c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used wasGoat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events wasperformed)
CF150, Polyclonal Antibody (Cat# AAA28789)
Full Name
CF150 Antibody (Center)
Gene Names
MB21D1; cGAS; h-cGAS; C6orf150
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(PLA2G2D Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
PLA2G2D, Polyclonal Antibody (Cat# AAA28718)
Full Name
PLA2G2D Antibody (C-term)
Gene Names
PLA2G2D; SPLASH; sPLA2S; PLA2IID; sPLA2-IID
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(SERPINH1 Antibody (Center) (Cat. #AAA28730) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SERPINH1, Polyclonal Antibody (Cat# AAA28730)
Full Name
SERPINH1 Antibody (Center)
Gene Names
SERPINH1; CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(TMPRSS2 Antibody (N-term) (RB18784) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TMPRSS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
TMPRSS2, Polyclonal Antibody (Cat# AAA28791)
Full Name
TMPRSS2 Antibody (N-term)
Gene Names
TMPRSS2; PP9284; PRSS10
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (L176), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry;clinical relevance has not been evaluated.)
ATG16L, Polyclonal Antibody (Cat# AAA28729)
Full Name
ATG16L Antibody
Gene Names
ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
Reactivity
Human, mouse
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(DRD2 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DRD2 antibody detected the DRD2 protein (arrow).)
DRD2, Polyclonal Antibody (Cat# AAA28691)
Full Name
DRD2 Antibody (C-term)
Gene Names
DRD2; D2R; D2DR
Reactivity
Human, mouse
Applications
Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(RGS1 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
RGS1, Polyclonal Antibody (Cat# AAA28739)
Full Name
RGS1 Antibody (N-term)
Gene Names
RGS1; 1R20; BL34; IER1; IR20; HEL-S-87
Reactivity
Human
Applications
Western Blot, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(ENOB Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
ENOB, Polyclonal Antibody (Cat# AAA28761)
Full Name
ENOB Antibody (Center)
Gene Names
ENO3; MSE; GSD13
Reactivity
Human, Mouse, Rat (Predicted Reactivity: Bovine, Pig)
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Pricing
FCM (Flow Cytometry)
(SLC5A12 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SLC5A12, Polyclonal Antibody (Cat# AAA28799)
Full Name
SLC5A12 Antibody (C-term)
Gene Names
SLC5A12; SMCT2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Pricing
SDS-PAGE
(Figure 11. Non-reduced and reduced SDS-PAGE of showing its purity.)
vimentin, Monoclonal Recombinant Antibody (Cat# AAA14574)
Full Name
Recombinant mouse anti vimentin
Reactivity
Human, mouse
Applications
Immunocytochemistry, Immunohistochemistry, Immunoblot
Purity
The purity of the antibody is >98% as determined by SDS-Polyacrylamide gel electrophoresis.
Pricing
IF (Immunofluorescence)
(LGR5/GPR49 Antibody (loop2) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).)
LGR5/GPR49, Polyclonal Antibody (Cat# AAA28712)
Full Name
LGR5/GPR49 Antibody (loop2)
Gene Names
LGR5; FEX; HG38; GPR49; GPR67; GRP49
Reactivity
Human, mouse, rat
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(KRAS Antibody (C-term)(AAA28748) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
KRAS, Polyclonal Antibody (Cat# AAA28748)
Full Name
KRAS Antibody (C-term)
Gene Names
KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
Reactivity
Human, Mouse, Rat (Predicted: Rat)
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(Flow Cytometry analyis of SSTR2 in human cell line. Human breast cancer cell line was stained with AAA14651 (filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 secondary antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.)
Somatostatin Receptor Type 2, Monoclonal Antibody (Cat# AAA14651)
Full Name
Somatostatin Receptor Type 2 (SSTr2)
Gene Names
SSTR2; SSTR2
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Flow Cytometry
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Pricing
FCM (Flow Cytometry)
(BCORL1 Antibody (N-term) (AAA28773) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
BCORL1, Polyclonal Antibody (Cat# AAA28773)
Full Name
BCORL1 Antibody (N-term)
Gene Names
BCORL1; BCoR-L1; CXorf10
Reactivity
Human
Applications
Flow Cytometry, Western Blot, Immunohistochemistry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(SCNN1A Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SCNN1A, Polyclonal Antibody (Cat# AAA28784)
Full Name
SCNN1A Antibody (Center)
Gene Names
SCNN1A; BESC2; ENaCa; SCNEA; SCNN1; ENaCalpha
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing