Search results for " nuclear"

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31192 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31192 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Rab4, Polyclonal Antibody (Cat# AAA31192)

Full Name

Rab4 Antibody

Gene Names

RAB4A; RAB4; HRES1; HRES-1; HRES-1/RAB4

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31223 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31223 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

SLIRP, Polyclonal Antibody (Cat# AAA31223)

Full Name

SLIRP Antibody

Gene Names

SLIRP; DC50; PD04872; C14orf156

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(83%), Bovine(83%), Horse(83%), Sheep(83%), Rabbit(83%), Dog(83%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

< >

<< Previous Next >>

Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IRF7, Polyclonal Antibody (Cat# AAA31330)

Full Name

Phospho-IRF7 (Ser471/Ser472) Antibody

Gene Names

IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

ETV2, Polyclonal Antibody (Cat# AAA27019)

Full Name

ETV2 Antibody

Gene Names

ETV2; ER71; ETSRP71

Reactivity

Human

Applications

Immunofluorescence

Purity

>95%, Protein G purified

Pricing

NRF2, Recombinant Protein (Cat# AAA11828)

Full Name

NRF2, 1-605aa, Human, His tag, E Coli

Gene Names

RAB3IL1; GRAB

Applications

SDS-PAGE

Purity

> 85% by SDS-PAGE

Pricing

< >

TOM20, Monoclonal Antibody (Cat# AAA28304)

Full Name

TOM20 Rabbit mAb

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with PrP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Prion Protein (PrP), Monoclonal Antibody (Cat# AAA30117)

Full Name

Prion Protein (PrP) Antibody

Gene Names

PRNP; CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31189 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31189 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Lgi1, Polyclonal Antibody (Cat# AAA31189)

Full Name

Lgi1 Antibody

Gene Names

LGI1; EPT; ETL1; ADLTE; ADPAEF; ADPEAF; IB1099; EPITEMPIN

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31195 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31195 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

P2X3, Polyclonal Antibody (Cat# AAA31195)

Full Name

P2X3 Antibody

Gene Names

P2RX3; P2X3

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(88%), Dog(100%)

Applications

Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31253 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31253 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Connexin 40/GJA5, Polyclonal Antibody (Cat# AAA31253)

Full Name

Connexin 40/GJA5 Antibody

Gene Names

GJA5; CX40; ATFB11

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

< >

<< Previous Next >>

Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Estrogen Receptor, alpha, Monoclonal Antibody (Cat# AAA23915)

Full Name

Estrogen Receptor, alpha (Marker of Estrogen Dependence)

Gene Names

ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1

Reactivity

Human

Applications

Immunofluorescence, Flow Cytometry, Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

< >

Akt, Polyclonal Antibody (Cat# AAA31035)

Full Name

Phospho-Akt (Ser124) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31178 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31178) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

CENPB, Polyclonal Antibody (Cat# AAA31178)

Full Name

CENPB Antibody

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

< >

<< Previous Next >>

Application Data (Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) in tau K18 P301L monomers over time. Thioflavin T ex = 450 nm, em = 485 nm.)

Tau, Active Protein (Cat# AAA27662)

Full Name

Active Human Recombinant Tau (K18), P301L Mutant Protein Monomer

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103

Applications

Western Blot

Purity

Purity: >95%
Purification: Ion-exchange Purified

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31225 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31225) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

KCNK9, Polyclonal Antibody (Cat# AAA31225)

Full Name

KCNK9 Antibody

Gene Names

KCNK9; KT3.2; TASK3; K2p9.1; TASK-3

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Chicken(83%), Xenopus(83%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31193 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31193 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

CHD4, Polyclonal Antibody (Cat# AAA31193)

Full Name

CHD4 Antibody

Gene Names

CHD4; CHD-4; Mi-2b; SIHIWES; Mi2-BETA

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Applications

Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31215 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31215 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

PDE11A, Polyclonal Antibody (Cat# AAA31215)

Full Name

PDE11A Antibody

Gene Names

PDE11A; PPNAD2

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

< >

MAP2, Polyclonal Antibody (Cat# AAA31338)

Full Name

MAP2 Antibody

Gene Names

MAP2; MAP2A; MAP2B; MAP2C

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

Peptide affinity purification

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Western Blot Analysis of human MCF-7 cell lysate using ER-beta1 Mouse Monoclonal Antibody (ERb455).)

ER-beta1 (Estrogen Receptor beta-1), Monoclonal Antibody (Cat# AAA13814)

Full Name

ER-beta1 (Estrogen Receptor beta-1) Mouse Monoclonal Antibody

Gene Names

ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IRAK1, Polyclonal Antibody (Cat# AAA31368)

Full Name

Phospho-IRAK1 (Thr209) Antibody

Gene Names

IRAK1; IRAK; pelle

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Horse (100%), Dog (82%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

< >

<< Previous Next >>

Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

Application Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12232)

Full Name

RAT ANTI TUBULIN ALPHA:HRP

Applications

Immunohistochemistry, Western Blot

Pricing

< >

<< Previous Next >>

Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

Application Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12009)

Full Name

RAT ANTI TUBULIN ALPHA

Applications

Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western Blot

Pricing

< >

RECA-1, Monoclonal Antibody (Cat# AAA12018)

Full Name

MOUSE ANTI RAT RECA-1

Applications

Immunohistochemistry, Immunofluorescence

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of Rat lung tissue using Nrf2 Polyclonal Antibody at dilution of 1:200.)

Nrf2, Polyclonal Antibody (Cat# AAA22058)

Full Name

Nrf2 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

Application Data (Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.)

FGF21, Polyclonal Antibody (Cat# AAA23563)

Full Name

FGF21 antibody - N-terminal region

Reactivity

Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry: Lung tissue (FFPE) stained with Rabbit anti-ER81 (Cat# AAA14073) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)

ER81, Polyclonal Antibody (Cat# AAA14073)

Full Name

Rabbit anti ER81 Polyclonal Antibody

Gene Names

ETV1; ER81

Reactivity

Mouse, Human, Rat

Applications

Flow Cytometry, Immunohistochemistry, Western Blot, Immunoprecipitation

Purity

Rabbit IgG is purified by Epitope Affinity Purification

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using PP2A-B56Î´/PR61Î´/PP2A-B56Î´/PR61Î´/PPP2R5D antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

PP2A-B56delta/PR61delta/PPP2R5D, Polyclonal Antibody (Cat# AAA28319)

Full Name

PP2A-B56delta/PR61delta/PPP2R5D Rabbit pAb

Gene Names

PPP2R5D; B56D

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

AKAP9/AKAP450/CG-NAP, Polyclonal Antibody (Cat# AAA13648)

Full Name

Goat anti-AKAP9/AKAP450/CG-NAP Antibody

Gene Names

AKAP9; LQT11; PRKA9; AKAP-9; CG-NAP; YOTIAO; AKAP350; AKAP450; PPP1R45; HYPERION; MU-RMS-40.16A

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse

Applications

Peptide ELISA, Western Blot, Immunofluorescence

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

NFkappaB p65, Monoclonal Antibody (Cat# AAA29642)

Full Name

NFkappaB p65 Mouse Monoclonal Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Rat, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification using immunogen.

Pricing

< >

Nesprin 1, Polyclonal Antibody (Cat# AAA31245)

Full Name

Nesprin 1 Antibody

Gene Names

SYNE1; 8B; AMCM; CPG2; ARCA1; EDMD4; KASH1; MYNE1; Nesp1; SCAR8; C6orf98; dJ45H2.2

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Horse(88%), Sheep(100%), Dog(88%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

< >

<< Previous Next >>

IHC (Immunohistochemistry) (AAA31121 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

FABP4, Polyclonal Antibody (Cat# AAA31121)

Full Name

FABP4 Antibody

Gene Names

FABP4; aP2; ALBP; AFABP; A-FABP; HEL-S-104

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31246 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31246) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

TROY, Polyclonal Antibody (Cat# AAA31246)

Full Name

TROY Antibody

Gene Names

TNFRSF19; TAJ; TROY; TRADE; TAJ-alpha

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Horse(88%), Sheep(86%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

< >

Hhip, Polyclonal Antibody (Cat# AAA31254)

Full Name

Hhip Antibody

Gene Names

HHIP; HIP

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry

Purity

Peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

< >

Exportin-5, Polyclonal Antibody (Cat# AAA31213)

Full Name

Exportin-5 Antibody

Gene Names

XPO5; exp5

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31272 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31272 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

DERL2, Polyclonal Antibody (Cat# AAA31272)

Full Name

DERL2 Antibody

Gene Names

DERL2; FLANa; F-LANa; CGI-101; F-LAN-1; derlin-2; DERtrin-2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31230 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31230 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

ACTR1A, Polyclonal Antibody (Cat# AAA31230)

Full Name

ACTR1A Antibody

Gene Names

ACTR1A; ARP1; Arp1A; CTRN1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

< >

OCT4, Monoclonal Antibody (Cat# AAA27015)

Full Name

OCT4 Monoclonal Antibody

Gene Names

POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

>95%
Protein G Purified

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (AAA31177 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31177 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Proteasome 20S LMP7, Polyclonal Antibody (Cat# AAA31177)

Full Name

Proteasome 20S LMP7 Antibody

Gene Names

PSMB8; JMP; ALDD; LMP7; NKJO; D6S216; PRAAS1; PSMB5i; RING10; D6S216E

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Horse(86%), Rabbit(100%), Dog(88%)

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using AAA28677 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

ATG7, Polyclonal Antibody (Cat# AAA28677)

Full Name

ATG7 Antibody (C-term)

Gene Names

ATG7; GSA7; APG7L; APG7-LIKE

Reactivity

Human, Mouse
Predicted reactivity: Chicken, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

p53, Polyclonal Antibody (Cat# AAA30976)

Full Name

Phospho-p53 (Ser392) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

< >

<< Previous Next >>

WB (Western Blot) (TGFBR2 Antibody western blot of mouse lung tissue lysates (35 ug/lane). The TGFBR2 antibody detected the TGFBR2 protein (arrow).)

TGFBR2, Polyclonal Antibody (Cat# AAA21269)

Full Name

Anti-TGFBR2 Antibody (aa13-40) IHC-plus

Gene Names

TGFBR2; AAT3; FAA3; LDS2; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TGFR-2; TGFbeta-RII

Reactivity

Mouse, Human

Applications

Immunohistochemistry, Immunofluorescence, Western Blot

Purity

Protein A purified

Pricing

< >

RECA-1, Monoclonal Antibody (Cat# AAA12019)

Full Name

MOUSE ANTI RAT RECA-1

Applications

Immunohistochemistry, Immunofluorescence

Pricing

< >

CD163, Monoclonal Antibody (Cat# AAA11943)

Full Name

MOUSE ANTI HUMAN CD163

Gene Names

CD163; M130; MM130

Reactivity

Guinea Pig, Pig, Rhesus Monkey, Sheep

Applications

Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoassay, Western Blot

Pricing

NCOR1, Monoclonal Antibody (Cat# AAA14104)

Full Name

Anti-NCOR1 Mouse mAb

Gene Names

NCOR1; N-CoR; TRAC1; N-CoR1; hN-CoR; PPP1R109

Reactivity

Human

Applications

Immunohistochemistry

Purity

Ascetic Fluid

Pricing

< >

PPAR-gamma, Polyclonal Antibody (Cat# AAA29649)

Full Name

PPAR-gamma (Phospho-Ser112) Polyclonal Antibody

Gene Names

PPARG; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescent analysis using the Antibody at 1:50 dilution)

MGEA5, Monoclonal Antibody (Cat# AAA30518)

Full Name

MGEA5 Antibody

Gene Names

MGEA5; OGA; MEA5; NCOAT

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence

Purity

Affinity-chromatography

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with Tyrosine Hydroxylase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was use as the secondary antibody)

Tyrosine Hydroxylase, Monoclonal Antibody (Cat# AAA30209)

Full Name

Tyrosine Hydroxylase Antibody

Gene Names

TH; TYH; DYT14; DYT5b

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Fig.6. Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1, PCNA Monoclonal Antibod y(1D7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)