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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
1719 results for "FC Antibody" - showing 1550-1600
FCM (Flow Cytometry)
(Flow Cytometric Analysis of Human Raji Cells. using HLA-Mouse Monoclonal Antibody followed by Goat anti-mouse IgG (Blue); Isotype control (Red).)
HLA-A, Monoclonal Antibody (Cat# AAA13821)
Full Name
HLA-A (MHC I) Mouse Monoclonal Antibody
Gene Names
HLA-B; AS; HLAB; Bw-47; Bw-50; SPDA1; B-4901; B-5001; HLA-Cw; HLA-B15; HLA-B39; HLA-B49; HLA-B50; HLA-B55; HLA-B59; HLA-B61; HEL-S-83; HLA-DRB1; HLA-B*45ZJ; HLA-B-3506; HLA-B-3905; HLA-B-5502; HLA-B-5602
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence
Pricing
Application Data
(Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Medium power)
CD1a, Monoclonal Antibody (Cat# AAA11890)
Full Name
MOUSE ANTI HUMAN CD1a:FITC
Gene Names
CD1A; R4; T6; CD1; FCB6; HTA1
Applications
Flow Cytometry
Pricing
Application Data
(Analysis of Protein Array containing >19,000 full-length human proteins using MSH6 Mouse Monoclonal Antibody (MSH6/3086) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
MSH6, Monoclonal Antibody (Cat# AAA23917)
Full Name
MSH6 (DNA Mismatch Repair Protein)
Gene Names
MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
FCM (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of RH35 cells using anti-Podoplanin/gp36/Pdpn antibody (AAA19233).Overlay histogram showing RH35 cells stained with AAA19233 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Podoplanin/gp36/Pdpn Antibody (AAA19233, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Podoplanin/gp36/Pdpn, Polyclonal Antibody (Cat# AAA19233)
Full Name
Anti-Podoplanin/gp36/Pdpn Antibody
Gene Names
Pdpn; T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse Beta-glucan Receptor: FITC)
DECTIN-1, Monoclonal Antibody (Cat# AAA12128)
Full Name
RAT ANTI MOUSE DECTIN-1:Biotin
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
Flow Cytometry
Pricing
Application Data
(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody , red an A and Mouse anti Rat CD8 MCA48), green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA11981)
Full Name
MOUSE ANTI RAT CD8 ALPHA
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA12084)
Full Name
MOUSE ANTI HUMAN CD105:RPE
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12188)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:Biotin
Applications
Flow Cytometry
Pricing
HLA Class 1 Antigen A23,A24, Monoclonal Antibody (Cat# AAA14677)
Full Name
HLA Class 1 Antigen A23,A24
Reactivity
Human
Applications
Cell Typing
Purity
Ascites
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with GLUT1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody.)
GLUT1, Monoclonal Antibody (Cat# AAA11692)
Full Name
Anti-GLUT1 Rabbit Monoclonal Antibody
Gene Names
SLC2A1; CSE; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT-1; SDCHCN; GLUT1DS
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity-chromatography
Pricing
FCM (Flow Cytometry)
(Cell Surface flow analysis of hCD73 in PBMC (Lymphocytes) using 0.2µg/106 cells of CD73 clone (ABM40E2). Green represents isotype control; red represents anti-hCD73 antibody. Goat anti-mouse PE conjugated secondary antibody was used.)
CD73, Monoclonal Antibody (Cat# AAA14868)
Full Name
CD73 Monoclonal Antibody
Gene Names
NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Protein G Chromatography
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD19: RPE- Alexa Fluor 647)
CD19, Monoclonal Antibody (Cat# AAA11870)
Full Name
MOUSE ANTI HUMAN CD19:FITC
Gene Names
CD19; B4; CVID3
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Wilms Tumor Protein, Monoclonal Antibody (Cat# AAA30123)
Full Name
Wilms Tumor Protein Antibody
Gene Names
WT1; GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
Affinity-chromatography
Pricing
FCM (Flow Cytometry)
(Flow cytometry analysis of PARP2 in U87MG cells. The cell was stained with AAA11732 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black))
PARP2, Monoclonal Antibody (Cat# AAA11732)
Full Name
PARP2 antibody
Gene Names
PARP2; ARTD2; ADPRT2; PARP-2; ADPRTL2; ADPRTL3; pADPRT-2
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Pricing
CIAS1/NALP3, Polyclonal Antibody (Cat# AAA11523)
Full Name
Anti-CIAS1/NALP3 antibody
Gene Names
NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL
Reactivity
Human, Rat; Predicted: Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry
Purity
Immunogen affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with MCM3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
MCM3, Monoclonal Antibody (Cat# AAA30464)
Full Name
MCM3 Antibody
Gene Names
MCM3; HCC5; P1.h; RLFB; P1-MCM3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry)
(CD99 Antibody in Immunohistochemistry (IHC (P)) -- Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with CD99 Monoclonal Antibody (12E7).)
CD99 / MIC2, Monoclonal Antibody (Cat# AAA13840)
Full Name
CD99 / MIC2 (Ewings Sarcoma Marker) Mouse Monoclonal Antibody
Gene Names
CD99; MIC2; HBA71; MIC2X; MIC2Y; MSK5X
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
Application Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE)
CD14, Monoclonal Antibody (Cat# AAA12179)
Full Name
MOUSE ANTI HUMAN CD14
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA27016 (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
PD-L1, Monoclonal Antibody (Cat# AAA27016)
Full Name
PD-L1 Monoclonal Antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
>95%, Protein G purified
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing HepG2 cells stained with AAA27041 (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
PKM, Monoclonal Antibody (Cat# AAA27041)
Full Name
PKM Monoclonal Antibody
Reactivity
Human, Rat, Mouse, Rabbit
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein G purified
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HepG2 cells using anti-ATF2 antibody (AAA11634).Overlay histogram showing HepG2 cells stained with AAA11634 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF2 Antibody (AAA11634,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Cyclic AMP-dependent transcription factor ATF-2, Polyclonal Antibody (Cat# AAA11634)
Full Name
Anti-ATF2 Antibody
Gene Names
ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse Beta-glucan Receptor: FITC)
DECTIN-1, Monoclonal Antibody (Cat# AAA12127)
Full Name
RAT ANTI MOUSE DECTIN-1:Biotin
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of 293T cells using anti-ARG2 antibody (AAA19256).Overlay histogram showing 293T cells stained with AAA19256 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARG2 Antibody (AAA19256, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ARG2, Polyclonal Antibody (Cat# AAA19256)
Full Name
Anti-ARG2 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of A549 cells using anti-MDR3 antibody (AAA11652).Overlay histogram showing A549 cells stained with AAA11652 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MDR3 Antibody (AAA11652,1ug/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ABCB4, Polyclonal Antibody (Cat# AAA11652)
Full Name
Anti-ABCB4 Antibody
Gene Names
ABCB4; GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen Affinity Purified
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA11933)
Full Name
MOUSE ANTI HUMAN CD105
Gene Names
ENG; END; HHT1; ORW1
Reactivity
Cynomolgus monkey, Horse, Monkey, Primate, Rhesus Monkey
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12094)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:Biotin
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12196)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12199)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(PYY Antibody (C-term) (AAA28733) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
PYY, Polyclonal Antibody (Cat# AAA28733)
Full Name
PYY Antibody (C-term)
Gene Names
PYY; PYY1; PYY-I
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(The human kidney tissue stained with Mouse GLEPP-1 monoclonal antibody (AAA14075) at 1:100 using AEC chromogen. (Note: formalin-fixed, paraffin-embedded sections need 15 minutes heat-induced epitope retrieval in 10 mM citrate buffer, pH 6.0, and 30 minutes incubation at room temperature with the primary antiibody).)
GLEPP-1, Monoclonal Antibody (Cat# AAA14075)
Full Name
Mouse anti GLEPP-1 Monoclonal Antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
The Mouse IgG is purified by Protein A-Afinity Chromatography according to isotyping.
Pricing
Affinity Assay
(The binding between immobilized S1RBD and the anti-SARS-CoV-2 S1RBD AAA13617.)
COVID 19 S1 Spike RBD Coronavirus, Antibody (Cat# AAA13617)
Full Name
Humanized Monoclonal Antibody against SARS-CoV-2 Spike Protein-1 Receptor-binding Domain (S1 RBD)
Reactivity
Viral
Applications
ELISA
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA28750 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28750, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events wasperformed.)
WNT5A, Polyclonal Antibody (Cat# AAA28750)
Full Name
WNT5A Antibody (Center)
Gene Names
WNT5A; hWNT5A
Reactivity
Human, Mouse, Rat
Predicted: Rabbit
Predicted: Rabbit
Applications
Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 2 ug/ml on h Spleen lysate.)
PD-L1, Monoclonal Antibody (Cat# AAA14873)
Full Name
Monoclonal Antibody to PD-L1 (Clone: ABM4E54)
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
Purified
Protein G Chromatography
Protein G Chromatography
Pricing
Application Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE)
CD14, Monoclonal Antibody (Cat# AAA11998)
Full Name
MOUSE ANTI HUMAN CD14
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of PFA-fixed HePG2 cells using Tumor Necrosis Factor (TNF alpha) Mouse Monoclonal Antibody (TNF706) followed by goat anti-mouse IgG-CF488 (green). Nuclei stained with RedDot.)
TNF-alpha (Tumor Necrosis Factor alpha), Monoclonal Antibody (Cat# AAA13806)
Full Name
TNF-alpha (Tumor Necrosis Factor alpha) Mouse Monoclonal Antibody
Gene Names
TNF; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
Reactivity
Human, Mouse, Rat, Rabbit, Cat, Dog, Zebrafish
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
WB (Western Blot)
(Western blot analysis of SLC10A1 using anti- SLC10A1 antibody (AAA11653).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates (positive control), Lane 2: rat kidney tissue lysates, (negative control)After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1 antigen affinity purified polyclonal antibody (AAA11653) at 0.25ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50KD. The expected band size for SLC10A1 is at 38KD.)
SLC10A1, Polyclonal Antibody (Cat# AAA11653)
Full Name
Anti-SLC10A1 Antibody
Gene Names
Slc10a1; Ntcp
Reactivity
Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Ki67 Mouse Monoclonal Antibody (MKI67/2466). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Ki-67, Monoclonal Antibody (Cat# AAA23909)
Full Name
Ki-67 (Proliferating Cell Marker)
Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human. Others not known.
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA12085)
Full Name
MOUSE ANTI HUMAN CD105
Gene Names
ENG; END; HHT1; ORW1
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11930)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
TCR gamma delta, Monoclonal Antibody (Cat# AAA17922)
Full Name
TCR gamma delta antibody
Reactivity
Recognizes the gamma/delta T-cell receptor (TCR).
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Pricing
T-Cell Receptor gamma delta, Monoclonal Antibody (Cat# AAA14565)
Full Name
Mouse anti T-Cell Receptor gamma delta
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded renal cancer tissues using CD203C mouse mAb with DAB staining.)
CD203C, Monoclonal Antibody (Cat# AAA27937)
Full Name
Mouse Monoclonal Antibody to CD203C
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry
Pricing
FCM (Flow Cytometry)
(Figure-1: Cell surface flowcytometry analysis of CLEC1on human PBMC (Monocytes) using 0.5 ug/10^6 Cells of Anti-CLEC1 antibody. Green represent isotype control and red represent Anti human CLEC1 antibody. Goat anti mouse PE conjugated was used as the secondary antibody.)
CLEC1, Monoclonal Antibody (Cat# AAA14882)
Full Name
Monoclonal Antibody to CLEC1 (Clone: ABM2H82)
Gene Names
CLEC1A; CLEC1; CLEC-1
Reactivity
Human
Applications
Flow Cytometry
Purity
Protein G Chromatography Purified
Pricing
Application Data
CD30, Monoclonal Antibody (Cat# AAA14859)
Full Name
anti-human CD30-R-PE
Reactivity
Human
Applications
Flow Cytometry
Pricing
WB (Western Blot)
(Western blot analysis of C3 expression in HepG2 cell lysate (AAA11693). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (stacking gel) / 90V (resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C3 monoclonal antibody (AAA11693) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (please inquire) with Tanon 5200 system. A specific band was detected for C3.)
C3/Complement C3, Monoclonal Antibody (Cat# AAA11693)
Full Name
Anti-C3/Complement C3 Rabbit Monoclonal Antibody
Gene Names
C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Affinity-chromatography
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with SF2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
SF2, Monoclonal Antibody (Cat# AAA30465)
Full Name
SF2 Antibody
Gene Names
SRSF1; ASF; SF2; SFRS1; SF2p33; SRp30a
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence Analysis of methanol-fixed human tonsil cryosection stained with CF488A CD3e clone RIV9 (green), mounted in EverBrite’ Mounting Medium with DAPI (nuclei, blue).)
CD3, Monoclonal Antibody (Cat# AAA13839)
Full Name
CD3 (T-Cell Marker) Mouse Monoclonal Antibody
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human, Mouse, Rat
Applications
Functional Assay, Flow Cytometry, Immunofluorescence
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating TUBB in Hela whole cell lysateLane 1: Mouse control IgG instead in Hela whole cell lysate.Lane 2: (2ug) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (5ug)For western blotting, the blot was detected at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:5000)
TUBB, Monoclonal Antibody (Cat# AAA28058)
Full Name
TUBB Monoclonal Antibody
Reactivity
Human, Rat, Rabbit, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein A purified
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA12029)
Full Name
MOUSE ANTI HUMAN CD105:RPE
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA12083)
Full Name
MOUSE ANTI HUMAN CD105:FITC
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing