Search results for "Control Antibody"

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WB (Western Blot) (ZEB2 antibody - middle region validated by WB using H9 human embryonic stem cells at 1:1000.)

ZEB2, Polyclonal Antibody (Cat# AAA23465)

Full Name

ZEB2 antibody - middle region

Gene Names

ZEB2; SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1

Reactivity

Cow, Guinea Pig, Horse, Human, Mouse, Pig, Rat, Zebrafish

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (GTF2I Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

GTF2I, Polyclonal Antibody (Cat# AAA28707)

Full Name

GTF2I Antibody (C-term)

Gene Names

GTF2I; WBS; DIWS; SPIN; IB291; BAP135; BTKAP1; TFII-I; WBSCR6; GTFII-I

Reactivity

Human (Predicted Reactivity: Rat)

Applications

Immunofluorescence, Immunohistochemistry, Flow Cytometry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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FABP-1/GOT2, Polyclonal Antibody (Cat# AAA19309)

Full Name

Anti-FABP-1/GOT2 Antibody

Gene Names

GOT2; KAT4; KATIV; mitAAT

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western blot analysis of ILK over-expressed 293 cell line, cotransfected with ILK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ILK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

ILK, Monoclonal Antibody (Cat# AAA24245)

Full Name

ILK (Integrin-linked Protein Kinase, 59kD Serine/Threonine-protein Kinase, ILK-1, ILK-2, p59ILK, ILK1, ILK2, DKFZp686F1765) (AP)

Gene Names

ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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ICC (Immunocytochemistry) (ICC staining SHP1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

SHP-1, Polyclonal Antibody (Cat# AAA29937)

Full Name

SHP-1 Antibody

Gene Names

PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IHC

Purity

Peptide affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-NTRK3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Liver)

NTRK3, Polyclonal Antibody (Cat# AAA23541)

Full Name

NTRK3 antibody - C-terminal region

Gene Names

NTRK3; TRKC; GP145-TrkC; gp145(trkC)

Reactivity

Cow, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish

Applications

WB

Purity

Affinity Purified

Pricing

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WB (Western Blot) (WB Suggested Anti-SUV420H1 AntibodyTitration: 1.25 ug/mlPositive Control: Jurkat Whole Cell)

KMT5B, Polyclonal Antibody (Cat# AAA23434)

Full Name

KMT5B Antibody - middle region

Gene Names

KMT5B; CGI85; MRD51; CGI-85; SUV420H1

Reactivity

Dog, Guinea Pig, Horse, Human, Mouse, Rat

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Western blot analysis of ENO3 over-expressed 293 cell line, cotransfected with ENO3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ENO3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

ENO3, Monoclonal Antibody (Cat# AAA24793)

Full Name

ENO3 (Enolase 3, 2-phospho-D-glycerate Hydro-lyase, beta-Enolase, Muscle-specific Enolase, MSE, Skeletal Muscle Enolase) (Biotin)

Gene Names

ENO3; MSE; GSD13

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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GNAS, Polyclonal Antibody (Cat# AAA23504)

Full Name

GNAS antibody - N-terminal region

Gene Names

GNAS; AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; PITA3; C20orf45

Reactivity

Cow, Human, Mouse, Pig, Rabbit, Rat

Applications

IHC, WB

Purity

Protein A purified

Pricing

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WB (Western Blot) (Western blot analysis of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

PGM3, Monoclonal Antibody (Cat# AAA25789)

Full Name

PGM3 (Phosphoacetylglucosamine Mutase, PAGM, Acetylglucosamine Phosphomutase, N-acetylglucosamine-phosphate Mutasem Phosphoglucomutase-3, AGM1, DKFZp434B187) (PE)

Gene Names

PGM3; AGM1; PAGM; IMD23; PGM 3

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of lysate from HepG2 cell line using PPT1 Antibody. AAA28640 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)

PPT1, Monoclonal Antibody (Cat# AAA28640)

Full Name

PPT1 Antibody

Gene Names

PPT1; PPT; CLN1; INCL

Reactivity

Human

Applications

WB, EIA, FC/FACS, IHC

Purity

Purified Mouse Monoclonal Antibody (Mab)

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of SW480 cells with Ret antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Ret, Monoclonal Antibody (Cat# AAA30185)

Full Name

Ret Antibody

Gene Names

RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IF, IHC, IP, FC/FACS

Purity

ProA affinity purified

Pricing

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Application Data (C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).)

FGFR2, Polyclonal Antibody (Cat# AAA26851)

Full Name

FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332) (AP)

Gene Names

FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM

Reactivity

Human, Monkey, Mouse, Rat

Applications

IF, EIA, IHC, WB

Purity

Purified by Protein G Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of 293T cells using anti-OTULIN antibody (AAA19319).Overlay histogram showing 293T cells stained with AAA19319 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OTULIN Antibody (AAA19319, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

OTULIN, Polyclonal Antibody (Cat# AAA19319)

Full Name

Anti-OTULIN Antibody

Gene Names

OTULIN; GUM; FAM105B

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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TAF4, Polyclonal Antibody (Cat# AAA19307)

Full Name

Anti-TAF4 Antibody

Gene Names

TAF4; TAF2C; TAF4A; TAF2C1; TAFII130; TAFII135

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Daudi cells with Tle6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

Tle6, Polyclonal Antibody (Cat# AAA29935)

Full Name

Tle6 Antibody

Gene Names

TLE6; GRG6

Reactivity

Human, Mouse

Applications

WB, ICC, IHC, FC/FACS

Purity

Peptide affinity purified.

Pricing

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WB (Western Blot) (Western blot analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

GATA2, Monoclonal Antibody (Cat# AAA24517)

Full Name

GATA2 (Endothelial Transcription Factor GATA-2, GATA-binding Protein 2, MGC2306) APC

Gene Names

GATA2; DCML; IMD21; NFE1B; MONOMAC

Reactivity

Human

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (TRIB2 monoclonal antibody Western Blot analysis of TRIB2 expression in Jurkat.)

TRIB2, Monoclonal Antibody (Cat# AAA24984)

Full Name

TRIB2 (Tribbles Homolog 2, TRB2, TRB-2, C5FW, GS3955) (Biotin)

Gene Names

TRIB2; C5FW; TRB2; GS3955

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (WB Suggested Anti-CHEK1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Small Intestine)

CHEK1, Polyclonal Antibody (Cat# AAA23414)

Full Name

CHEK1 antibody - middle region

Gene Names

CHEK1; CHK1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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WB (Western Blot) (ALS2CR8 monoclonal antibody, Western Blot analysis of ALS2CR8 expression in Hela NE.)

ALS2CR8, Monoclonal Antibody (Cat# AAA24727)

Full Name

ALS2CR8 (CARF, Amyotrophic Lateral Sclerosis 2 Chromosomal Region Candidate Gene 8 Protein, Calcium-response Factor, Testis Development Protein NYD-SP24, DKFZp667N246, FLJ12675, FLJ21579) (Biotin)

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Nuclear Factor I/C, Monoclonal Antibody (Cat# AAA25773)

Full Name

Nuclear Factor I/C (NFIC, CCAAT-binding Transcription Factor, CTF, Nuclear Factor 1 C-Type, CTF5, MGC20153, NF-I, NF1-C, CTF, TGGCA-Binding Protein, NFI) (PE)

Gene Names

NFIC; CTF; NFI; CTF5; NF-I

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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ICC (Immunocytochemistry) (ICC staining VCP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

VCP, Polyclonal Antibody (Cat# AAA29948)

Full Name

VCP Antibody

Gene Names

VCP; p97; TERA; ALS14; IBMPFD

Reactivity

Human, Mouse, Zebrafish

Applications

WB, ICC, IHC

Purity

Peptide affinity purified

Pricing

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WB (Western Blot) (Western blot analysis of BIRC5 over-expressed 293 cell line, cotransfected with BIRC5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BIRC5 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Survivin, Monoclonal Antibody (Cat# AAA25562)

Full Name

Survivin (Apoptosis Inhibitor 4, API4, Apoptosis Inhibitor Survivin, Baculoviral IAP Repeat Containing Protein 5, BIRC5, EPR-1, IAP4) (HRP)

Gene Names

BIRC5; API4; EPR-1

Reactivity

Human

Applications

EIA, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of ABCF2 over-expressed 293 cell line, cotransfected with ABCF2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ABCF2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

ABCF2, Monoclonal Antibody (Cat# AAA25297)

Full Name

ABCF2 (ATP-binding Cassette Sub-family F Member 2, Iron-inhibited ABC Transporter 2, HUSSY-18, DKFZp586K1823, EST133090, M-ABC1) (HRP)

Gene Names

ABCF2; ABC28; HUSSY18; HUSSY-18; EST133090

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (WB Suggested Anti-HNRPL Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysateHNRNPL is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells)

HNRPL, Polyclonal Antibody (Cat# AAA23474)

Full Name

HNRPL antibody - N-terminal region

Gene Names

HNRNPL; HNRPL; hnRNP-L; P/OKcl.14

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

IF, IP, IHC, WB

Purity

Affinity Purified

Pricing

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SAMHD1, Monoclonal Antibody (Cat# AAA19355)

Full Name

Anti-SAMHD1 Antibody (monoclonal, 3B9)

Gene Names

SAMHD1; DCIP; CHBL2; HDDC1; MOP-5; SBBI88

Reactivity

Human

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western blot analysis of SNX1 over-expressed 293 cell line, cotransfected with SNX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SNX1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

SNX1, Monoclonal Antibody (Cat# AAA25258)

Full Name

SNX1 (Sorting Nexin-1, MGC8664) (FITC)

Gene Names

SNX1; VPS5; HsT17379

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DUSP6 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

DUSP6, Monoclonal Antibody (Cat# AAA24785)

Full Name

DUSP6 (Dual Specificity Protein Phosphatase 6, Dual Specificity Protein Phosphatase PYST1, Mitogen-activated Protein Kinase Phosphatase 3, MAP Kinase Phosphatase 3, MKP3, MKP-3, PYST1) (Biotin)

Gene Names

DUSP6; HH19; MKP3; PYST1

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (ALS2CR8 monoclonal antibody, Western Blot analysis of ALS2CR8 expression in Hela NE.)

ALS2CR8, Monoclonal Antibody (Cat# AAA25320)

Full Name

ALS2CR8 (CARF, Amyotrophic Lateral Sclerosis 2 Chromosomal Region Candidate Gene 8 Protein, Calcium-response Factor, Testis Development Protein NYD-SP24, DKFZp667N246, FLJ12675, FLJ21579) (HRP)

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of NDN over-expressed 293 cell line, cotransfected with NDN Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NDN monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

NDN, Monoclonal Antibody (Cat# AAA25761)

Full Name

NDN (Necdin) (PE)

Gene Names

NDN; PWCR; HsT16328

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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ICC (Immunocytochemistry) (ICC staining HDAC9 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

HDAC9, Monoclonal Antibody (Cat# AAA30407)

Full Name

HDAC9 Antibody

Gene Names

HDAC9; HD7; HD9; HD7b; HDAC; HDRP; MITR; HDAC7; HDAC7B; HDAC9B; HDAC9FL

Reactivity

Human, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Purity

ProA affinity purified

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)

Hsp70/Hsc70, Monoclonal Antibody (Cat# AAA17780)

Full Name

Hsp70/Hsc70 Antibody: Biotin

Gene Names

HSPA2; HSP70

Reactivity

Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast

Applications

WB, IP, IHC

Pricing

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WB (Western Blot) (BUB1B monoclonal antibody Western Blot analysis of BUB1B expression in Hela NE.)

BUBR1, Monoclonal Antibody (Cat# AAA24739)

Full Name

BUBR1 (Mitotic Checkpoint Serine/Threonine-protein Kinase BUB1 beta, MAD3/BUB1-related Protein Kinase, hBUBR1, Mitotic Checkpoint Kinase MAD3L, Protein SSK1, BUB1B, MAD3L, SSK1) (Biotin)

Gene Names

BUB1B; MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon cancer using AAA18859 at dilution of 1:100)

Cellular tumor antigen p53, Polyclonal Antibody (Cat# AAA18859)

Full Name

Rabbit anti-human Cellular tumor antigen p53 polyclonal Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

EIA, WB, IHC

Purity

Caprylic Acid Ammonium Sulfate Precipitation Purified

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HEPA1-6 cells using anti-PAR4/Pawr antibody (AAA19278).Overlay histogram showing HEPA1-6 cells stained with AAA19278 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAR4/Pawr Antibody (AAA19278, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PAR4/Pawr, Polyclonal Antibody (Cat# AAA19278)

Full Name

Anti-PAR4/Pawr Antibody

Gene Names

Pawr; PAR4; Par-4; 2310001G03Rik

Reactivity

Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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CDC27, Polyclonal Antibody (Cat# AAA19285)

Full Name

Anti-CDC27 Antibody

Gene Names

CDC27; APC3; HNUC; NUC2; ANAPC3; CDC27Hs; D0S1430E; D17S978E

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of TAGLN/Transgelin using anti-TAGLN/Transgelin antibody (AAA19286).TAGLN/Transgelin was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TAGLN/Transgelin Antibody (AAA19286) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

TAGLN/Transgelin, Polyclonal Antibody (Cat# AAA19286)

Full Name

Anti-TAGLN/Transgelin Antibody

Gene Names

TAGLN; SM22; SMCC; TAGLN1; WS3-10

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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Application Data (Published customer image: Mouse anti Human CD49d antibody, clone HP2/1 used for binding efficiency determinationImage caption:Binding efficiencies (BE) of different a4beta7 molecules composed of distinct a4 mutants to monoclonal antibodies against a4, beta7 or the a4beta7 heterodimer. Binding efficiency is determined by the ratio between the mean fluorescence of antibody binding to each a4 molecule and of the binding in a mock-transfected cell culture (see Materials and Methods for details). Dark gray bars represent binding to the human (wild type) a4 clone, whereas light gray bars are those of binding to the different a4 mutants (as shown in the x-axis). a4 mutants which included substitutions at codon 201 are boxed. A, binding of anti-a4 2b4 antibody. B, binding of anti-a4 HP2/1 antibody. C, BE of different anti-a4 and beta7 antibodies to the human a4 and the quintuple a4 mutant (5 aa mut). Bars represent the range of standard errors deduced from triplicate experiments. p-values of Student's t tests are shown above each comparison. NS, non-significant (> 0.05).From:Darc M, Hait SH, Soares EA, Cicala C, Seuanez HN, et al. (2011) Polymorphisms in the a4 Integrin of Neotropical Primates: Insights for Binding of Natural Ligands and HIV-1 gp120 to the Human a4beta7. PLoS ONE 6(9): e24461.)

Application Data (Published customer image: Mouse anti Human CD49d antibody, clone HP2/1 used for western blotting and immunoprecipitationImage caption:Tetraspanins CD81, CD82 and CD151 are associated with a4beta1 throughout erythroid maturation and with beta3 in proerythroblasts and basophilic erythroblasts. A. CD81, CD82 and CD151 precipitates from Mn2+-activated proerythroblasts (ProEB, day 5), basophilic (BasoEB, day 8) and polychromatic (PolyEB, day 12) erythroblasts were successively probed with anti-a4, anti-beta1 and anti-beta3 antibodies; tetraspanin controls from each time point are also illustrated. All tetraspanins co-precipitated a4 and beta1 from erythroblasts B. Tetraspanin precipitates from day 6 proerythroblasts (ProEB) solubilised in the presence of EDTA or different cations, and from Mn2+-activated basophilic erythroblasts (BasoEB, day 8) were probed with a mix of antibodies to a5, beta1, beta2 and beta3 integrins while the control samples were probed with the relevant tetraspanin antibodies. For clarity, integrin controls are illustrated for the EDTA blot but were present on all blots. beta1 and beta3 integrins were precipitated well only in the presence of Mn2+. C. CD81 and CD82 precipitates from day 5 proerythroblasts were successively probed with different anti-integrin subunit antibodies and demonstrate co-precipitation of beta1 and beta3 but not a5 or beta2 integrins. D. CD81 (454720) and CD82 (53H5) precipitates from day 6 proerythroblasts (ProEB) and HEL cells (HEL) solubilised in the presence of EDTA, Ca2++Mg2+ or Mn2+ probed with anti-CD82 and anti-CD81 antibodies. Each tetraspanin co-precipitates the other most strongly in the presence of Mn2+ from proerythroblasts while any cation permits co-precipitation in HEL cells. Integrins were analysed on 7.5% gels, tetraspanins on 12% gels; non-reducing conditions. Unless stated, the following clones were used: CD53, MEM-53; CD63, MEM-259; CD81, 454720; CD82, TS82b; CD151, IIG5a; a4, HP2/1; a5, IIA1; aL, TS1/22; aIIb, PAB-1. All day 5 and 6 cultures comprised 90 -95% proerythroblasts; day 8 culture comprised 5% proerythroblasts, 81% basophilic erythroblasts and 14% polychromatic erythroblasts; day 12 culture comprised 41% polychromatic erythroblasts, 15% orthochromatic erythroblasts and 41% reticulocytes. In the day 5 and 6 cultures 15 -34% of cells were GPA+ and 28 -35% of cells were aIIb+. Day 8 and day 12 cultures had 77% and 97% GPA+ cells, respectively, and 9% and 0% aIIb+ cells, respectively.From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, et al. (2013) Tetraspanins CD81 and CD82 Facilitate a4beta1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1. PLoS ONE 8(5): e62654.)

CD49d, Monoclonal Antibody (Cat# AAA12002)

Full Name

MOUSE ANTI HUMAN CD49d

Gene Names

ITGA4; IA4; CD49D

Reactivity

Bovine, Cat, Cynomolgus monkey, Goat, Horse, Llama, Mink, Mustelid, Pig, Rabbit, Rat, Rhesus Monkey

Applications

Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation

Pricing

MARC1, Polyclonal Antibody (Cat# AAA23536)

Full Name

MARC1 Antibody - C-terminal region

Gene Names

MARC1; MOSC1

Reactivity

Tested Reactivity: Human
Predicted Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Pricing

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WB (Western Blot) (CDK2 monoclonal antibody. Western Blot analysis of CDK2 expression in Jurkat.)

CDK2, Monoclonal Antibody (Cat# AAA25052)

Full Name

CDK2 (Cell Division Protein Kinase 2, p33 Protein Kinase) (FITC)

Gene Names

CDK2; CDKN2; p33(CDK2)

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of TCF19 over-expressed 293 cell line, cotransfected with TCF19 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TCF19 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

TCF19, Monoclonal Antibody (Cat# AAA24977)

Full Name

TCF19 (Transcription Factor 19, TCF-19, SC1, SC1-1, Transcription Factor SC1) (Biotin)

Gene Names

TCF19; SC1; TCF-19

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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TRK Fused Gene, Monoclonal Antibody (Cat# AAA30204)

Full Name

TRK Fused Gene Antibody

Gene Names

TFG; TF6; HMSNP; SPG57; TRKT3

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IF, IHC, IP

Purity

ProA affinity purified

Pricing

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WB (Western Blot) (Western blot analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

CDC25C, Monoclonal Antibody (Cat# AAA24754)

Full Name

CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) (Biotin)

Gene Names

CDC25C; CDC25; PPP1R60

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot analysis of TUBA1 expression in transfected 293T cell line by TUBA1 monoclonal antibody. Lane 1: TUBA1 transfected lysate (49.9kD). Lane 2: Non-transfected lysate.)

Tubulin alpha-4A Chain, Monoclonal Antibody (Cat# AAA24988)

Full Name

Tubulin alpha-4A Chain (TUBA4A, Alpha-tubulin 1, Tubulin alpha-1 Chain, TUBA1, Testis-specific alpha-tubulin, Tubulin H2-alpha) (Biotin)

Gene Names

TUBA4A; ALS22; TUBA1; H2-ALPHA

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of POU4F3 over-expressed 293 cell line, cotransfected with POU4F3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POU4F3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

POU4F3, Monoclonal Antibody (Cat# AAA25507)

Full Name

POU4F3 (POU Domain, Class 4, Transcription Factor 3, Brain-specific Homeobox/POU Domain Protein 3C, Brain-3C, Brn-3C, BRN3C, MGC138412) (HRP)

Gene Names

POU4F3; BRN3C; DFNA15; DFNA42; DFNA52

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (PROX-1-S514 Antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

PROX-1-S514, Polyclonal Antibody (Cat# AAA28650)

Full Name

PROX-1-S514 Antibody

Reactivity

Human, mouse

Applications

WB, EIA, IHC, FC/FACS

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)

Hsp70/Hsc70, Monoclonal Antibody (Cat# AAA17784)

Full Name

Hsp70/Hsc70 Antibody: ATTO 390

Gene Names

HSPA2; HSP70

Reactivity

Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast

Applications

WB, IP, IHC

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of PP2A-alpha/PPP2CA using anti-PP2A-alpha/PPP2CA antibody (AAA19366).PP2A-alpha/PPP2CA was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PP2A-alpha/PPP2CA Antibody (AAA19366) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)