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Application
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2304 results for " secondary" - showing 1450-1500
IHC (Immunohistochemistry)
(Figure 8. IHC analysis of HBD using anti-HBD antibody (AAA19142).HBD was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HBD Antibody (AAA19142) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
HBD, Polyclonal Antibody (Cat# AAA19142)
Full Name
Anti-HBD Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3225).)
Prohibitin, Monoclonal Antibody (Cat# AAA23933)
Full Name
Prohibitin (Mitochondrial Marker)
Gene Names
PHB; PHB1; HEL-215; HEL-S-54e
Reactivity
Human
Applications
WB, IF, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of K562 cells using anti-PDIA6 antibody (AAA19282).Overlay histogram showing K562 cells stained with AAA19282 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDIA6 Antibody (AAA19282, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PDIA6, Polyclonal Antibody (Cat# AAA19282)
Full Name
Anti-PDIA6 Antibody
Gene Names
PDIA6; P5; ERP5; TXNDC7
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-NRF1 antibody Titration: 1 ug/mLSample Type: Human heart)
NRF1, Polyclonal Antibody (Cat# AAA23433)
Full Name
NRF1 Antibody - middle region
Gene Names
NRF1; ALPHA-PAL
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
WB, IHC
Purity
Affinity Purified
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Calretinin Mouse Monoclonal Antibody (CALB2/2602). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Calretinin/Calbindin 2, Monoclonal Antibody (Cat# AAA23938)
Full Name
Calretinin/Calbindin 2 (Mesothelioma Marker)
Gene Names
CALB2; CR; CAL2; CAB29
Reactivity
Human
Applications
WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of K562 cells using anti-MCM6 antibody (AAA19372).Overlay histogram showing K562 cells stained with AAA19372 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- MCM6 Antibody (AAA19372, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MCM6, Monoclonal Antibody (Cat# AAA19372)
Full Name
Anti-MCM6 Antibody (monoclonal, 10I9)
Gene Names
MCM6; Mis5; P105MCM; MCG40308
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
ROR-gamma/RORC, Monoclonal Antibody (Cat# AAA23951)
Full Name
ROR-gamma/RORC (RAR-related Orphan Receptor C)
Gene Names
RORC; TOR; RORG; RZRG; IMD42; NR1F3; RZR-GAMMA
Reactivity
Human
Applications
FC, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
anti-Rabbit IgG+HRP, Secondary Antibody (Cat# AAA13854)
Full Name
anti-Rabbit IgG+HRP
Reactivity
Rabbit
Applications
Western Blot, Immunohistochemistry
Purity
Goats were immunized with highly purified Rabbit IgG
Affinity purified and conjugated with horseradish peroxidase (HRP)
Affinity purified and conjugated with horseradish peroxidase (HRP)
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Cytokeratin 15, Monoclonal Antibody (Cat# AAA23923)
Full Name
Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker)
Gene Names
KRT15; K15; CK15; K1CO
Reactivity
Human
Applications
FC/FACS, IF, WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of MED15 using anti-MED15 antibody (AAA19169).MED15 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED15 Antibody (AAA19169) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
MED15/Pcqap, Polyclonal Antibody (Cat# AAA19169)
Full Name
Anti-MED15/Pcqap Picoband Antibody
Gene Names
MED15; TIG1; CAG7A; CTG7A; PCQAP; TIG-1; TNRC7; ARC105
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using CAIX-Monospecific Mouse Monoclonal Antibody (CA9/3406). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Renal Cell Carcinoma, Monoclonal Antibody (Cat# AAA23952)
Full Name
Renal Cell Carcinoma (Carbonic Anhydrase IX)
Gene Names
CA9; MN; CAIX
Reactivity
Human
Applications
FC/FACS, IHC, WB
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Goat Anti-Human IgG, Monkey ads, Secondary Antibody (Cat# AAA14913)
Full Name
Goat Anti-Human IgG, Monkey ads-UNLB
Applications
ELISA, FLISA
Purity
Affinity chromatography on human IgG covalently linked to agarose
Pricing
Goat F(ab')2 Anti-Human IgG (gamma chain specific), Polyclonal Secondary Antibody (Cat# AAA14907)
Full Name
Goat F(ab')2 Anti-Human IgG-FITC
Applications
Flow Cytometry
Pricing
Goat anti Human IgG Fc, Secondary Antibody (Cat# AAA14316)
Full Name
Goat anti Human IgG Fc
Reactivity
Minimal cross-reaction to Bovine, Horse, and Mouse Serum Proteins.
Purity
Human IgG Fc antibody was purified by affinity chromatography.
Pricing
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power)
CD169, Monoclonal Antibody (Cat# AAA11973)
Full Name
MOUSE ANTI RAT CD169
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
Application Data
(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power)
CD169, Monoclonal Antibody (Cat# AAA11972)
Full Name
MOUSE ANTI RAT CD169
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using SMO Polyclonal Antibody (AAA10769) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
SMO, Polyclonal Antibody (Cat# AAA10769)
Full Name
smo
Gene Names
SMO; Gx; SMOH; FZD11
Applications
Western Blot
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling PIEZO1 with AAA13800 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
PIEZO1, Monoclonal Antibody (Cat# AAA13800)
Full Name
PIEZO1 Mouse mAb
Gene Names
PIEZO1; DHS; Mib; LMPH3; FAM38A
Reactivity
Homo Sapiens
Applications
Immunoprecipitation, Immunofluorescence, Flow Cytometry
Pricing
WB (Western Blot)
(Fig1: Western blot analysis of Annexin A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: A431 cell lysateLane 2: Rat uterus tissue lysate)
Annexin A1 Autoantibody (Anti-ANXA1), ELISA Kit (Cat# AAA10593)
Full Name
Human Annexin A1 Autoantibody (Anti-ANXA1) ELISA Kit
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-251MG cells using VHL Polyclonal Antibody (AAA10644) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
VHL, Polyclonal Antibody (Cat# AAA10644)
Full Name
VHL Polyclonal Antibody
Gene Names
VHL; RCA1; VHL1; pVHL; HRCA1
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(AAA31072 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
LKB1, Polyclonal Antibody (Cat# AAA31072)
Full Name
Phospho-LKB1 (Ser428) Antibody
Gene Names
STK11; PJS; LKB1; hLKB1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human rectal cancer using MMP25 antibody at dilution of 1:200 (40x lens).)
MMP25, Polyclonal Antibody (Cat# AAA10688)
Full Name
MMP25 Polyclonal Antibody
Gene Names
MMP25; MMP20; MMPL1; MMP-25; MMP20A; MT6MMP; MTMMP6; MT-MMP6; MT6-MMP; MT-MMP 6
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IF (Immunoflouorescence)
(Immunofluorescence analysis of HeLa cells using DiMethyl-Histone H3-K27 Rabbit pAb (AAA10663) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
H3K27me2, Antibody (Cat# AAA10663)
Full Name
Histone H3K27me2 Polyclonal Antibody
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Immunocytochemistry, Dot Blot, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of GPR84 expression in A431 whole cell lysates)
GPR84, Polyclonal Antibody (Cat# AAA31105)
Full Name
GPR84 Antibody
Gene Names
GPR84; EX33; GPCR4
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(Figure 8 Immunohistochemistry Validation of ATF6 in Rat Brain Tissue Immunohistochemical analysis of paraffin-embedded Rat brain tissue using anti-ATF6 antibody (AAA10937) at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
ATF6, Polyclonal Antibody (Cat# AAA10937)
Full Name
ATF6 Antibody
Gene Names
ATF6; ATF6A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
ATF6 Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
(Western blot of STAG2 expression in HeLa nuclear extract with STAG2 monoclonal antibody, clone 3C6.)
STAG2, Monoclonal Antibody (Cat# AAA12354)
Full Name
Mouse Monoclonal [clone 3C6] (IgG1,k) to Human STAG2
Gene Names
STAG2; SA2; SA-2; SCC3B; bA517O1.1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified from ascites by Protein A
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human brain using Myelin Basic Protein Rabbit pAb (AAA10723) at dilution of 1:100 (40x lens).)
MBP, Polyclonal Antibody (Cat# AAA10723)
Full Name
MBP Polyclonal Antibody
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human trachea using ATPIF1 antibody at dilution of 1:100 (40x lens).)
ATPIF1, Polyclonal Antibody (Cat# AAA10772)
Full Name
ATPIF1 Polyclonal Antibody
Gene Names
ATPIF1; IP; ATPI; ATPIP
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using BMPR1A antibody (AAA10766) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced KitExposure Time: 90s)
BMPR1A, Polyclonal Antibody (Cat# AAA10766)
Full Name
BMPR1A Polyclonal Antibody
Gene Names
BMPR1A; ALK3; SKR5; CD292; ACVRLK3; 10q23del
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using MID1 antibody (AAA28180). Blue: DAPI for nuclear staining.)
MID1, Polyclonal Antibody (Cat# AAA28180)
Full Name
MID1 Polyclonal Antibody
Gene Names
MID1; OS; FXY; OSX; OGS1; XPRF; BBBG1; GBBB1; MIDIN; RNF59; ZNFXY; TRIM18
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using CYP24A1 antibody at dilution of 1:200 (40x lens).)
CYP24A1, Polyclonal Antibody (Cat# AAA10721)
Full Name
CYP24A1
Gene Names
CYP24A1; CP24; HCAI; CYP24; P450-CC24
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of U2OS cells using TOMM40 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)
TOMM40, Polyclonal Antibody (Cat# AAA10745)
Full Name
TOMM40 Polyclonal Antibody
Gene Names
TOMM40; TOM40; PEREC1; C19orf1; PER-EC1; D19S1177E
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(EIF2S1 Antibody (N-term) (Cat. #AAA28744)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF2S1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
EIF2S1, Polyclonal Antibody (Cat# AAA28744)
Full Name
EIF2S1 Antibody (N-term)
Gene Names
EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
Reactivity
Mouse (Predicted Reactivity: Zebrafish, Bovine, Chicken, Drosophila, Pig, Rat, Yeast)
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(Figure 12 Immunohistochemistry Validation of PD-L1in Human Tumors (Gadiot et al., 2011)Immunohistochemical analysis of patient tumors labeling PD-L1 with anti-PD-L1 antibodies (AAA10941). Several anti-PD-L1 antibodies were tested for staining, “Only 1 antibody gave no background staining and was competitively blocked by the addition of PD-L1Fc protein (AAA10941)”.)
PDL-1, Polyclonal Antibody (Cat# AAA10941)
Full Name
PDL-1 Antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
PD-L1 Antibody is affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-emebedded human placenta using H3R17me2a antibody (AAA10654) at dilution of 1:100 (40x lens).)
H3R17me2a, Antibody (Cat# AAA10654)
Full Name
Histone H3R17me2a Polyclonal Antibody
Gene Names
HIST1H3J; H3/j; H3FJ
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(AAA31248 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31248 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PDE1C, Polyclonal Antibody (Cat# AAA31248)
Full Name
PDE1C Antibody
Gene Names
PDE1C; Hcam3; DFNA74; hCam-3; cam-PDE 1C
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Horse(100%), Rabbit(88%), Dog(100%)
Predicted Reactivity: Pig(88%), Horse(100%), Rabbit(88%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Endothelin 1 Rabbit pAb (A0686) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
EDN1, Antibody (Cat# AAA10719)
Full Name
EDN1 Polyclonal Antibody
Gene Names
EDN1; ET1; QME; PPET1; ARCND3; HDLCQ7
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Ephrin A5 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Ephrin A5, Polyclonal Antibody (Cat# AAA27798)
Full Name
Anti-Ephrin A5 Antibody
Gene Names
EFNA5; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Rabbit
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Flow Cytometry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded Human lung cancer using CEBPA Rabbit pAb (AAA10634) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
C/EBPalpha, Polyclonal Antibody (Cat# AAA10634)
Full Name
C/EBPalpha
Gene Names
CEBPA; CEBP; C/EBP-alpha
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using NGF antibody (AAA10624) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure Time: 180s)
NGF, Polyclonal Antibody (Cat# AAA10624)
Full Name
NGF Polyclonal Antibody
Gene Names
NGF; NGFB; HSAN5; Beta-NGF
Applications
Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of MUC2 using HT-29 whole cell lysates)
MUC2, Polyclonal Antibody (Cat# AAA31137)
Full Name
MUC2 Antibody
Gene Names
MUC2; MLP; SMUC; MUC-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain issue labeling NPTX2 with AAA10933 at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (red) and DAPI staining (blue).)
NPTX2, Polyclonal Antibody (Cat# AAA10933)
Full Name
NPTX2 Antibody
Gene Names
NPTX2; NP2; NARP; NP-II
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
NPTX2 Antibody is affinity chromatography purified via peptide column.
Pricing
ELISA
(ELISA analysis was performed by loading 100 uL per well of an anti-Human Fab (CH1) mAb at a concentration of 5, 2.5, 1.25, 0.625, 0.3125, 0.15625, 0.078, and 0.039 ug/mL across 100 ng per well of a human antibody pre-coated plate. The plate was incubated for 1 hour at 37°C. Detection was performed using TMB Substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.)
Fab (CH1), Monoclonal Secondary Antibody (Cat# AAA27977)
Full Name
Anti-Human Fab (CH1) mAb, HRP
Applications
Western Blot
Pricing
Goat F(ab) Anti-Human IgG (gamma chain specific), Secondary Antibody (Cat# AAA14905)
Full Name
Goat Fab Anti-Human IgG-UNLB
Reactivity
Human
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of mouse brain cells using GFAP Rabbit pAb (AAA10625) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
GFAP, Polyclonal Antibody (Cat# AAA10625)
Full Name
GFAP Polyclonal Antibody
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of Mouse leg bone using CAMP Rabbit pAb (AAA10689) at dilution of 1:100. Blue: DAPI for nuclear staining.)
CAMP, Polyclonal Antibody (Cat# AAA10689)
Full Name
CAMP Polyclonal Antibody
Gene Names
CAMP; LL37; CAP18; CRAMP; HSD26; CAP-18; FALL39; FALL-39
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western BlotPositive WB detected in: U87 whole cell lysateAll lanes: ATAT1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 45, 46, 47, 38, 37, 36, 34 kDaObserved band size: 47 kDa)
ATAT1, Polyclonal Antibody (Cat# AAA27001)
Full Name
ATAT1 Antibody
Gene Names
ATAT1; TAT; MEC17; C6orf134; Nbla00487; alpha-TAT; alpha-TAT1
Reactivity
Human
Applications
Western Blot
Purity
>95%, Protein G purified
Pricing
IHC (Immunohistochemistry)
(Anti-TCR Gamma/Delta antibody IHC of mouse spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
TCR Gamma+TCR Delta, Monoclonal Antibody (Cat# AAA12360)
Full Name
Armenian Hamster Monoclonal [clone GL-3] (IgG) to Mouse TCR Gamma+TCR Delta
Reactivity
Mouse
Applications
Immunohistochemistry, Flow Cytometry
Purity
Protein G Purified
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human stomach using OLR1 Antibody at dilution of 1:200 (40x lens).)
OLR1, Polyclonal Antibody (Cat# AAA10753)
Full Name
OLR1 Polyclonal Antibody
Gene Names
OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
ED-B domain of fibronectin, Monoclonal Recombinant Antibody (Cat# AAA14185)
Full Name
Anti-ED-B domain of fibronectin [A2]
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein A affinity purified
Pricing