Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
152 results for " regulation" - showing 100-150
WB (Western Blot)
(Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3194).)
Prohibitin, Monoclonal Antibody (Cat# AAA23932)
Full Name
Prohibitin (Mitochondrial Marker)
Gene Names
PHB; PHB1; HEL-215; HEL-S-54e
Reactivity
Human
Applications
WB, IF, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Standard Curve (Sample)
interleukin 2, ELISA Kit (Cat# AAA15403)
Full Name
Rabbit Interleukin 2, IL-2 ELISA Kit
Gene Names
IL2; TCGF
Reactivity
Rabbit
Pricing
WB (Western Blot)
(WB Suggested Anti-ESR2 antibody Titration: 1 ug/mLSample Type: Human liver)
ESR2, Polyclonal Antibody (Cat# AAA23449)
Full Name
ESR2 antibody - N-terminal region
Gene Names
ESR2; Erb; ESRB; ODG8; ESTRB; NR3A2; ER-BETA; ESR-BETA
Reactivity
Human, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-PPP3CA Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Muscle)
PPP3CA, Polyclonal Antibody (Cat# AAA23542)
Full Name
PPP3CA antibody - middle region
Gene Names
PPP3CA; CALN; CCN1; CNA1; CALNA; IECEE; PPP2B; ACCIID; CALNA1; IECEE1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
Standard Curve (Sample)
Sirtuin 1 (SIRT1), ELISA Kit (Cat# AAA22423)
Full Name
Human Sirtuin 1 (SIRT1) ELISA Kit
Gene Names
sirt1; sir2l1; sirtuin
Reactivity
Human
Pricing
Standard Curve (Sample)
Sirtuin 1 (SIRT1), ELISA Kit (Cat# AAA22360)
Full Name
Rat Sirtuin 1 (SIRT1) ELISA Kit
Gene Names
sirt1; sir2l1; sirtuin
Reactivity
Rat
Pricing
WB (Western Blot)
(WB Suggested Anti-PHB Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysatePHB is supported by BioGPS gene expression data to be expressed in Jurkat)
PHB, Polyclonal Antibody (Cat# AAA23420)
Full Name
PHB antibody - C-terminal region
Gene Names
PHB; PHB1; HEL-215; HEL-S-54e
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
WB, IHC
Purity
Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-YAP1 Antibody Titration: 1 ug/mlPositive Control: 721_B cell lysate)
YAP1, Polyclonal Antibody (Cat# AAA23538)
Full Name
YAP1 antibody - C-terminal region
Gene Names
YAP1; YAP; YKI; COB1; YAP2; YAP65
Reactivity
Tested Reactivity: Human
Predicted Reactivity: Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish
Predicted Reactivity: Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of NSF using anti-NSF antibody (AAA19136).NSF was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NSF Antibody (AAA19136) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
NSF, Polyclonal Antibody (Cat# AAA19136)
Full Name
Anti-NSF Picoband antibody
Gene Names
NSF; SKD2; SEC18
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
WB (Western Blot)
(WB Suggested Anti-VEZF1 Antibody Titration: 1 ug/mlPositive Control: 293T cells lysateThere is BioGPS gene expression data showing that VEZF1 is expressed in HEK293T)
VEZF1, Polyclonal Antibody (Cat# AAA23448)
Full Name
VEZF1 antibody - middle region
Gene Names
VEZF1; DB1; ZNF161
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(P15RS monoclonal antibody. Western Blot analysis of P15RS expression in NIH/3T3.)
P15RS, Monoclonal Antibody (Cat# AAA24299)
Full Name
P15RS (RPRD1A, Regulation of Nuclear Pre-mRNA Domain-containing Protein 1A, Cyclin-dependent Kinase Inhibitor 2B-related Protein, p15INK4B-related Protein, FLJ10656, HsT3101, MGC19513) (AP)
Gene Names
RPRD1A; P15RS; HsT3101
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Host: RabbitTarget Name: CELF2Sample Type: JurkatAntibody Dilution: 1.0ug/mlCELF2 is strongly supported by BioGPS gene expression data to be expressed in Jurkat)
CELF2, Polyclonal Antibody (Cat# AAA23473)
Full Name
CELF2 Antibody - N-terminal region
Gene Names
CELF2; ETR3; ETR-3; NAPOR; CELF-2; CUGBP2; BRUNOL3; CUG-BP2
Reactivity
Human
Applications
WB
Purity
Affinity purified
Pricing
WB (Western Blot)
(WB Suggested Anti-RXRA Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Transfected 293T)
RXRA, Polyclonal Antibody (Cat# AAA23431)
Full Name
RXRA antibody - N-terminal region
Gene Names
RXRA; NR2B1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat
Applications
WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-RXRA Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysateRXRA is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells)
RXRA, Polyclonal Antibody (Cat# AAA23509)
Full Name
RXRA antibody - N-terminal region
Gene Names
RXRA; NR2B1
Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Rat
Applications
WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-CREB3L2 Antibody Titration: 1.25ug/mlELISA Titer: 1:12500Positive Control: Jurkat cell lysate)
CREB3L2, Polyclonal Antibody (Cat# AAA23437)
Full Name
CREB3L2 antibody - N-terminal region
Gene Names
CREB3L2; BBF2H7
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Protein A purified
Pricing
WB (Western Blot)
(WB Suggested Anti-OTX2 Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysate)
OTX2, Polyclonal Antibody (Cat# AAA23410)
Full Name
OTX2 antibody - N-terminal region
Gene Names
OTX2; CPHD6; MCOPS5
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
SDS-PAGE
(SDS-PAGE Analysis Purified ATG5 Mouse Monoclonal Antibody (ATG5/2492). Confirmation of Purity and Integrity of Antibody.)
ATG5, Monoclonal Antibody (Cat# AAA23947)
Full Name
ATG5 (Autophagy Marker)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
WB (Western Blot)
(WB Suggested Anti-RPS14 Antibody Titration: 2.5ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysate)
RPS14, Polyclonal Antibody (Cat# AAA23472)
Full Name
RPS14 antibody - middle region
Gene Names
RPS14; S14; EMTB
Reactivity
Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
Applications
WB
Purity
Protein A purified
Pricing
WB (Western Blot)
(WB Suggested Anti-EYA1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain)
EYA1, Polyclonal Antibody (Cat# AAA23409)
Full Name
EYA1 antibody - middle region
Gene Names
EYA1; BOP; BOR; BOS1; OFC1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-THOC1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate)
THOC1, Polyclonal Antibody (Cat# AAA23479)
Full Name
THOC1 Antibody - C-terminal region
Gene Names
THOC1; P84; HPR1; P84N5
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-EED Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:2500Positive Control: OVCAR-3 cell lysateEED is strongly supported by BioGPS gene expression data to be expressed in Human OVCAR3 cells)
EED, Polyclonal Antibody (Cat# AAA23445)
Full Name
EED antibody - N-terminal region
Gene Names
EED; HEED; COGIS; WAIT1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IF, WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-JAZF1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysate)
JAZF1, Polyclonal Antibody (Cat# AAA23453)
Full Name
JAZF1 antibody - N-terminal region
Gene Names
JAZF1; TIP27; ZNF802
Reactivity
Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3225).)
Prohibitin, Monoclonal Antibody (Cat# AAA23933)
Full Name
Prohibitin (Mitochondrial Marker)
Gene Names
PHB; PHB1; HEL-215; HEL-S-54e
Reactivity
Human
Applications
WB, IF, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
WB (Western Blot)
(WB Suggested Anti-NRF1 antibody Titration: 1 ug/mLSample Type: Human heart)
NRF1, Polyclonal Antibody (Cat# AAA23433)
Full Name
NRF1 Antibody - middle region
Gene Names
NRF1; ALPHA-PAL
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
WB, IHC
Purity
Affinity Purified
Pricing
Application Data
(Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.)
HEY1, Polyclonal Antibody (Cat# AAA23413)
Full Name
HEY1 antibody - C-terminal region
Gene Names
HEY1; CHF2; OAF1; HERP2; HESR1; HRT-1; NERP2; hHRT1; BHLHb31
Reactivity
Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Applications
IHC, WB
Purity
Protein A purified
Pricing
WB (Western Blot)
(WB Suggested Anti-ZNF238 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Pancreas)
ZNF238, Polyclonal Antibody (Cat# AAA23447)
Full Name
ZNF238 antibody - N-terminal region
Gene Names
ZBTB18; RP58; MRD22; TAZ-1; ZNF238; C2H2-171
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
alpha 1 Microglobulin, Native Protein (Cat# AAA14346)
Full Name
alpha 1 Microglobulin protein
Gene Names
AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
Reactivity
Human
Purity
> 98% (by SDS-PAGE)< br>Highly Pure
Pricing
FCM (Flow Cytometry)
(MUSK Antibody (AAA28697) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
MUSK, Polyclonal Antibody (Cat# AAA28697)
Full Name
MUSK Antibody
Gene Names
MUSK; CMS9
Reactivity
Human, mouse
Applications
Western Blot, Flow Cytometry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (PAB)
Pricing
FCM (Flow Cytometry)
(Flow Cytometric Analysis of PFA-fixed K562 cells using Calponin-1 Recombinant Mouse Monoclonal Antibody (rCNN1/832) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
Calponin-1, Monoclonal Antibody (Cat# AAA13848)
Full Name
Calponin-1 (Smooth Muscle Marker)
Gene Names
CNN1; SMCC; Sm-Calp; HEL-S-14
Reactivity
Human, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
WB (Western Blot)
(Host: Rabbit Target Name: FOSL1 Sample Tissue: Human HepG2 Whole Cell Antibody Dilution: 1ug/ml)
FOSL1, Polyclonal Antibody (Cat# AAA23383)
Full Name
FOSL1 antibody - middle region
Gene Names
FOSL1; FRA; FRA1; fra-1
Reactivity
Tested Species Reactivity Human, Rat
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(MCOLN1 Antibody (C-term) (AAA28690)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MCOLN1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
MCOLN1, Polyclonal Antibody (Cat# AAA28690)
Full Name
MCOLN1 Antibody (C-term)
Gene Names
MCOLN1; ML4; MG-2; MLIV; MST080; TRPML1; MSTP080; TRP-ML1; TRPM-L1
Reactivity
Human
Predicted Reactivity: Monkey, Mouse
Predicted Reactivity: Monkey, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ICOS-L Mouse Monoclonal Antibody (ICOSL/3111). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
ICOS-L/ICOS Ligand/B7RP-1 (Immuno-Oncology Target), Monoclonal Antibody (Cat# AAA23916)
Full Name
ICOS-L/ICOS Ligand/B7RP-1 (Immuno-Oncology Target)
Gene Names
ICOSLG; B7h; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml
Pricing
FCM (Flow Cytometry)
(WB - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail FC - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail Overlay histogram showing HepG2 cells stained with AAA28757(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28757, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA28757)
Full Name
MCSF Receptor (CSF1R) Antibody (C-term)
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(PCSK9 Antibody (N-term) (Cat. #AAA28774) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell(top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
PCSK9, Polyclonal Antibody (Cat# AAA28774)
Full Name
PCSK9 Antibody (N-term)
Gene Names
PCSK9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Pricing
IHC (Immunohistchemistry)
(AAA31073 at 1/100 staining Carcinoid tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 107°C.)
SIRT3, Polyclonal Antibody (Cat# AAA31073)
Full Name
SIRT3 Antibody
Gene Names
SIRT3; SIR2L3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Peptide ELISA
Purity
Peptide affinity purification
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12081)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
Western Blot
Pricing
Application Data
(AAA31434 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31434 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
Smad3, Polyclonal Antibody (Cat# AAA31434)
Full Name
Phospho-Smad3 (Ser423+Ser425) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
BRK, Polyclonal Antibody (Cat# AAA31371)
Full Name
Phospho-BRK (Tyr447) Antibody
Gene Names
PTK6; BRK
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse (89%)
Predicted Reactivity: Horse (89%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11930)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11864)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
Immunofluorescence
Pricing
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
HRI, Polyclonal Antibody (Cat# AAA28727)
Full Name
HRI Antibody (N-term)
Gene Names
EIF2AK1; HCR; HRI
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12211)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed, paraffin-embedded Rat Heart stained with SUMO-2 Monoclonal Antibody (SUMO2/1199))
SUMO-2, Monoclonal Antibody (Cat# AAA13825)
Full Name
SUMO-2 Mouse Monoclonal Antibody
Gene Names
SUMO3; SMT3A; Smt3B; SMT3H1; SUMO-3
Reactivity
Human, Rat.
Predicted to show a broad species reactivity
Predicted to show a broad species reactivity
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11850)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
Immunohistochemistry, Western Blot
Pricing
Coronavirus, Antigen (Cat# AAA14531)
Full Name
Coronavirus, Canine Antigen
Applications
ELISA
Pricing
IHC (Immunohistochemistry)
(AAA30967 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Lamin A/C, Polyclonal Antibody (Cat# AAA30967)
Full Name
Phospho-Lamin A/C (Ser392) Antibody
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(CHAK1 Antibody (AAA28787) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHAK1 antibody detected the CHAK1 protein (arrow).)
TRPM7 (CHAK1), Polyclonal Antibody (Cat# AAA28787)
Full Name
TRPM7 (CHAK1) Antibody (C-term)
Gene Names
TRPM7; CHAK; CHAK1; ALSPDC; LTRPC7; LTrpC-7; TRP-PLIK
Reactivity
Human, Mouse
Predicted: Rat
Predicted: Rat
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(WB of with THP on 10% 50S gel. 1:250 antibody dilution in OiluObuffer for 1 hour at room temperature. Apparent MW is 95 kDa .)
Progesterone Receptor, Polyclonal Antibody (Cat# AAA14415)
Full Name
Progesterone Receptor Antibody BIOTIN
Gene Names
PGR; PR; NR3C3
Reactivity
Cat, Human, Monkey, Pig
Applications
Immunoprecipitation, Western Blot
Pricing
FCM (Flow Cytometry)
(KRAS Antibody (C-term)(AAA28748) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
KRAS, Polyclonal Antibody (Cat# AAA28748)
Full Name
KRAS Antibody (C-term)
Gene Names
KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
Reactivity
Human, Mouse, Rat (Predicted: Rat)
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data
(Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.)
SLC26A3, Polyclonal Antibody (Cat# AAA23387)
Full Name
SLC26A3 antibody - middle region
Gene Names
SLC26A3; CLD; DRA
Reactivity
Tested Reactivity: Human
Predicted Reactivity: Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep
Predicted Reactivity: Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Pricing