Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
155 results for " NF " - showing 100-150
Standard Curve (Sample)
Interleukin 33, IL-33, ELISA Kit (Cat# AAA11316)
Full Name
Canine Interleukin 33, IL-33 ELISA Kit
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Reactivity
Canine
Pricing
Standard Curve (Sample)
NF-kappaB p65, ELISA Kit (Cat# AAA21580)
Full Name
Rat NF-kappaB p65(Nuclear Factor-kappaB P65) ELISA Kit
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Reactivity
Rat
Pricing
Application Data
(Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
YY1, Monoclonal Antibody (Cat# AAA26041)
Full Name
YY1 (YY1 Transcription Factor, DELTA, INO80S, NF-E1, UCRBP, YIN-YANG-1) (APC)
Gene Names
YY1; DELTA; NF-E1; UCRBP; GADEVS; INO80S; YIN-YANG-1
Applications
IF, IHC, WB
Purity
Purified
Pricing
Application Data
(Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
YY1, Monoclonal Antibody (Cat# AAA26575)
Full Name
YY1 (YY1 Transcription Factor, DELTA, INO80S, NF-E1, UCRBP, YIN-YANG-1) (PE)
Gene Names
YY1; DELTA; NF-E1; UCRBP; GADEVS; INO80S; YIN-YANG-1
Applications
IF, IHC, WB
Purity
Purified
Pricing
Standard Curve (Sample)
Neurofilament light polypeptide (NEFL), ELISA Kit (Cat# AAA27232)
Full Name
Rat Neurofilament light polypeptide (NEFL) ELISA Kit
Gene Names
NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
Reactivity
Rat
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using PEX14 antibody. Blue: DAPI for nuclear staining.)
PEX14, Polyclonal Antibody (Cat# AAA28157)
Full Name
PEX14
Gene Names
PEX14; NAPP2; PBD13A; Pex14p; dJ734G22.2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Pricing
Application Data
(Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
YY1, Monoclonal Antibody (Cat# AAA26308)
Full Name
YY1 (YY1 Transcription Factor, DELTA, INO80S, NF-E1, UCRBP, YIN-YANG-1) (FITC)
Gene Names
YY1; DELTA; NF-E1; UCRBP; GADEVS; INO80S; YIN-YANG-1
Applications
IF, IHC, WB
Purity
Purified
Pricing
SDS-PAGE
Neurofilament light polypeptide (Nefl), Recombinant Protein (Cat# AAA18436)
Full Name
Recombinant Mouse Neurofilament light polypeptide (Nefl)
Gene Names
Nefl; Nfl; NF-L; NF68; CMT2E; AI847934
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Standard Curve (Sample)
Cytochrome P450 3A4, ELISA Kit (Cat# AAA22704)
Full Name
Human Cytochrome P450 3A4 (CYP3A4) ELISA Kit
Gene Names
CYP3A4; HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1
Reactivity
Human
Pricing
Standard Curve (Sample)
Nuclear factor erythroid 2-related factor 2, NRF2, ELISA Kit (Cat# AAA19022)
Full Name
Mouse Nuclear factor erythroid 2-related factor 2, NRF2 ELISA Kit
Gene Names
NFE2L2; NRF2; HEBP1; IMDDHH
Reactivity
Mouse
Pricing
Standard Curve (Sample)
Neurofilament, Light Polypeptide (NEFL), ELISA Kit (Cat# AAA13905)
Full Name
Mouse Neurofilament, Light Polypeptide (NEFL) ELISA Kit
Gene Names
Nefl; Nfl; NF-L; NF68; CMT2E; AI847934
Reactivity
Mouse
Pricing
Standard Curve (Sample)
interleukin 33, ELISA Kit (Cat# AAA15607)
Full Name
Human Interleukin 33, IL-33 ELISA Kit
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Reactivity
Human
Pricing
Standard Curve (Sample)
Interleukin 33 (IL33), ELISA Kit (Cat# AAA20610)
Full Name
Rat Interleukin 33 (IL33) ELISA Kit
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Reactivity
Rat
Pricing
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])
NFKB1, Monoclonal Antibody (Cat# AAA26094)
Full Name
NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1, DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NFKB-p105, NFKB-p50, p105, p50) (APC)
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1
Applications
IF, IHC, WB, Cell
Purity
Purified
Pricing
Standard Curve (Sample)
Interleukin 33 (IL-33), ELISA Kit (Cat# AAA12426)
Full Name
Human Interleukin 33 (IL-33) ELISA Kit
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Reactivity
Human
Pricing
Application Data
(Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
YY1, Monoclonal Antibody (Cat# AAA26042)
Full Name
YY1 (YY1 Transcription Factor, DELTA, INO80S, NF-E1, UCRBP, YIN-YANG-1) (APC)
Gene Names
YY1; DELTA; NF-E1; UCRBP; GADEVS; INO80S; YIN-YANG-1
Applications
IF, IHC, WB
Purity
Purified
Pricing
Standard Curve (Sample)
Interleukin 12, ELISA Kit (Cat# AAA16316)
Full Name
Rabbit Interleukin 12 ELISA Kit
Gene Names
IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
Reactivity
Rabbit
Pricing
Application Data
(Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
YY1, Monoclonal Antibody (Cat# AAA26573)
Full Name
YY1 (YY1 Transcription Factor, DELTA, INO80S, NF-E1, UCRBP, YIN-YANG-1) (PE)
Gene Names
YY1; DELTA; NF-E1; UCRBP; GADEVS; INO80S; YIN-YANG-1
Applications
IF, IHC, WB
Purity
Purified
Pricing
Application Data
(Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.)
NFKB1, Monoclonal Antibody (Cat# AAA26565)
Full Name
NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1, DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NFKB-p105, NFKB-p50, p105, p50) (PE)
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1
Applications
IF, IHC, WB
Purity
Purified
Pricing
Standard Curve (Sample)
Interleukin 33/ST2, ELISA Kit (Cat# AAA16681)
Full Name
Human Interleukin 33/ST2 ELISA Kit
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26; DKFZp586H0523; RP11-575C20.2
Reactivity
Human
Pricing
SDS-PAGE
(SDS-PAGE analysis of recombinant IL-33 fragment on Coomassie Blue-stained 4-20% gradient gel.)
IL-33, Recombinant Protein (Cat# AAA10966)
Full Name
IL-33 Recombinant Protein
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Applications
Western Blot
Purity
~95%
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using RELB mouse mAb with DAB staining.)
RELB, Monoclonal Antibody (Cat# AAA14155)
Full Name
Anti-RELB Mouse mAb
Gene Names
RELB; IREL; I-REL; REL-B
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Flow Cytometry
Purity
Ascitic Fluid
Pricing
ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CARD8 mAb. [Lot No. 2108C2a-1])
CARD8, Monoclonal Antibody (Cat# AAA10604)
Full Name
Mouse monoclonal antibody Anti-Human CARD8
Gene Names
CARD8; NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Flow Cytometry, Immunocytochemistry
Purity
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Pricing
IF (Immunofluorescence)
(AAA31063 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
NF kappaB p65, Polyclonal Antibody (Cat# AAA31063)
Full Name
Phospho-NF kappaB p65 (Ser281) Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from Hela and MCF cells using NRF2 Mouse mAb at 1:1000 dilution. Predicted band size: 68KDa. Observed band size: 68kDa.)
NRF2, Monoclonal Antibody (Cat# AAA14162)
Full Name
Anti-NRF2 Mouse mAb
Gene Names
NFE2L2; NRF2; HEBP1
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Anti-NF200 antibody (monoclonal), AAA11507, IHC(P)IHC(P): Rat Brain Tissue)
NF200, Monoclonal Antibody (Cat# AAA11507)
Full Name
Anti-NF200 antibody (monoclonal)
Gene Names
NEFH; NFH
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(AAA30928 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Nrf2, Polyclonal Antibody (Cat# AAA30928)
Full Name
Nrf2 Antibody
Gene Names
NFE2L2; NRF2; HEBP1; IMDDHH
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
Application Data
(Fig-1: Induction of MDA5 activity by poly(I: C) prepacked with lipofectamine in MDA5 Leeporter HEK293T cells.)
MDA5/NF-kBT, Cell Line (Cat# AAA14874)
Full Name
MDA5/NF-kB Leeporter Luciferase Reporter-HEK293T Cell Line
Applications
Functional Assay
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis using the Antibody at 1:50 dilution.)
NRF2, Monoclonal Antibody (Cat# AAA29775)
Full Name
NRF2 (Phospho-S40) Antibody
Gene Names
NFE2L2; NRF2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
S100A9, Monoclonal Antibody (Cat# AAA13837)
Full Name
S100A9 (Macrophage Marker) Mouse Monoclonal Antibody
Gene Names
S100A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
Reactivity
Human and Rat. Others not tested.
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
Application Data
(Published customer image: Effect of clustered ephrin-A1-Fc on vascular formation in the rat striatum. Clustered ephrin-A1-Fc was injected into the lesioned side of the lateral ventricle in the unilaterally lesioned rats. Brains taken 6 weeks after injection were sectioned coronally and stained for GFAP (green) and RECA-1 (red) and with DAPI (nuclei; blue). The rectangular insets are shown in Fig. 8B. Scale bar: 100 um.From: Jing X, Miwa H, Sawada T, Nakanishi I, Kondo T, et al. (2012) Ephrin-A1-Mediated Dopaminergic Neurogenesis and Angiogenesis in a Rat Model of Parkinson's Disease. PLoS ONE 7(2): e32019.)
RECA-1, Monoclonal Antibody (Cat# AAA12018)
Full Name
MOUSE ANTI RAT RECA-1
Applications
Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IRAK1, Polyclonal Antibody (Cat# AAA31368)
Full Name
Phospho-IRAK1 (Thr209) Antibody
Gene Names
IRAK1; IRAK; pelle
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse (100%), Dog (82%)
Predicted Reactivity: Horse (100%), Dog (82%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12081)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
Western Blot
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of Rat lung tissue using Nrf2 Polyclonal Antibody at dilution of 1:200.)
Nrf2, Polyclonal Antibody (Cat# AAA22058)
Full Name
Nrf2 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
NFkappaB p65, Monoclonal Antibody (Cat# AAA29642)
Full Name
NFkappaB p65 Mouse Monoclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification using immunogen.
Pricing
Application Data
(Published customer image: Effect of clustered ephrin-A1-Fc on vascular formation in the rat striatum. Clustered ephrin-A1-Fc was injected into the lesioned side of the lateral ventricle in the unilaterally lesioned rats. Brains taken 6 weeks after injection were sectioned coronally and stained for GFAP (green) and RECA-1 (red) and with DAPI (nuclei; blue). The rectangular insets are shown in Fig. 8B. Scale bar: 100 um.From: Jing X, Miwa H, Sawada T, Nakanishi I, Kondo T, et al. (2012) Ephrin-A1-Mediated Dopaminergic Neurogenesis and Angiogenesis in a Rat Model of Parkinson's Disease. PLoS ONE 7(2): e32019.)
RECA-1, Monoclonal Antibody (Cat# AAA12019)
Full Name
MOUSE ANTI RAT RECA-1
Applications
Immunohistochemistry, Immunofluorescence
Pricing
Application Data
(AAA31382 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (AAA31382 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
NF kappaB p105/p50, Polyclonal Antibody (Cat# AAA31382)
Full Name
Phospho-NF kappaB p105/p50 (Ser923) Antibody
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IRAK1, Polyclonal Antibody (Cat# AAA31367)
Full Name
Phospho-IRAK1 (Thr100) Antibody
Gene Names
IRAK1; IRAK; pelle
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Fig-3: CpG ODNs did not show any TLR4 agonist activity. TLR4/IL-8Leeporter™ HeLa cells were treated with various CpG ODNs at 100 ug/mlas well as a positive TLR4 agonist, LPS, at 10 ng/ml for 16 h, and luciferase activitywas then analyzed.)
TLR9, Ligand (Cat# AAA14883)
Full Name
CpG ODN (2006), TLR9 ligand (Class B)
Gene Names
TLR9; CD289
Reactivity
Human
Applications
Functional Assay
Pricing
IHC (Immunohistochemistry)
(AAA31013 at 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
GATA1, Polyclonal Antibody (Cat# AAA31013)
Full Name
Phospho-GATA1 (Ser142) Antibody
Gene Names
GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
ATF6, Polyclonal Antibody (Cat# AAA31376)
Full Name
Phospho-ATF6 (Thr166) Antibody
Gene Names
ATF6; ATF6A
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Horse (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Horse (100%), Rabbit (100%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11930)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11864)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
Immunofluorescence
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12211)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
IF (Immunofluorescence)
(AAA30936 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
NF kappaB p65, Polyclonal Antibody (Cat# AAA30936)
Full Name
Phospho-NF kappaB p65 (Ser536) Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
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IHC (Immunohistochemistry)
(AAA30934 at 1/100 staining human TB tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
I kappaB alpha, Polyclonal Antibody (Cat# AAA30934)
Full Name
Phospho-I kappaB alpha (Ser32/Ser36) Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PAR4, Polyclonal Antibody (Cat# AAA31375)
Full Name
Phospho-PAR4 (Thr163) Antibody
Gene Names
PAWR; PAR4; Par-4
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Product Limitations
GATA3, Monoclonal Antibody (Cat# AAA13571)
Full Name
GATA3
Gene Names
GATA3; cGATA-3
Reactivity
Human
Predicted: Rat
Predicted: Rat
Applications
Immunohistochemistry
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11850)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
Immunohistochemistry, Western Blot
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with TNF using NFkappaB-p65 (Phospho-Ser276) Antibody.)
NFkappaB-p65, Polyclonal Antibody (Cat# AAA29703)
Full Name
NFkappaB-p65(Phospho-Ser276) Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
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