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Type
Reactivity
Gene Name
Isotype
Host
Application
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158 results for " Myeloid" - showing 100-150
Application Data
(Detection limit for recombinant GST tagged RUNX1 is approximately 1ng/ml as a capture antibody.)
RUNX1, Monoclonal Antibody (Cat# AAA26613)
Full Name
RUNX1 (Runt-Related Transcription Factor 1, AML1, AML1-EVI-1, AMLCR1, CBFA2, EVI-1, PEBP2aB) (PE)
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1
Applications
IF, IHC, WB
Purity
Purified
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of CD13/ANPEP using anti-CD13/ANPEP antibody (AAA19371).CD13/ANPEP was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CD13/ANPEP Antibody (AAA19371) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
CD13/ANPEP, Monoclonal Antibody (Cat# AAA19371)
Full Name
Anti-CD13/ANPEP Antibody (monoclonal, 5B9)
Gene Names
ANPEP; APN; CD13; LAP1; P150; PEPN; GP150
Reactivity
Human, Rat, Monkey
Applications
WB, IHC-P
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with RUNX1+RUNX2+RUNX3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
RUNX1+RUNX2+RUNX3, Monoclonal Antibody (Cat# AAA30202)
Full Name
RUNX1+RUNX2+RUNX3 Antibody
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, IP, FC/FACS
Purity
ProA affinity purified
Pricing
Standard Curve (Sample)
Procalcitonin (PCT), ELISA Kit (Cat# AAA12785)
Full Name
Mouse Procalcitonin (PCT) ELISA Kit
Gene Names
S100a9; Mrp14
Reactivity
Mouse
Pricing
Standard Curve (Sample)
Induced myeloid leukemia cell differentiation protein Mcl-1, ELISA Kit (Cat# AAA17552)
Full Name
Human Induced myeloid leukemia cell differentiation protein Mcl-1 ELISA Kit
Gene Names
MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
Reactivity
Human
Pricing
Standard Curve (Sample)
Runt-Related Transcription Factor 1, ELISA Kit (Cat# AAA23695)
Full Name
Human Runt-Related Transcription Factor 1 ELISA Kit
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
Reactivity
Human
Pricing
Application Data
(Detection limit for recombinant GST tagged IFI16 is ~0.3ng/ml as a capture antibody.)
IFI16, Monoclonal Antibody (Cat# AAA24244)
Full Name
IFI16 (Interferon gamma-inducible Protein 16, Gamma-interferon-inducible Protein 16, Ifi-16, IFNGIP1, Interferon-inducible Myeloid Differentiation Transcriptional Activator, MGC9466, PYHIN2) (AP)
Gene Names
IFI16; PYHIN2; IFNGIP1
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Detection limit for recombinant GST tagged IFI16 is ~0.3ng/ml as a capture antibody.)
IFI16, Monoclonal Antibody (Cat# AAA24540)
Full Name
IFI16 (Interferon gamma-inducible Protein 16, Gamma-interferon-inducible Protein 16, Ifi-16, IFNGIP1, Interferon-inducible Myeloid Differentiation Transcriptional Activator, MGC9466, PYHIN2) APC
Gene Names
IFI16; PYHIN2; IFNGIP1
Reactivity
Human
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Standard Curve (Sample)
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell. [antibody concentration 10 ug/ml])
RUNX2, Monoclonal Antibody (Cat# AAA26111)
Full Name
RUNX2 (Runt-Related Transcription Factor 2, AML3, CBFA1, CCD, CCD1, MGC120022, MGC120023, OSF2, PEA2aA, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1) (APC)
Gene Names
RUNX2; CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1
Applications
IF, IHC, WB
Purity
Purified
Pricing
Standard Curve (Sample)
Interferon Gamma Inducible Protein 16, ELISA Kit (Cat# AAA16171)
Full Name
Human Interferon Gamma Inducible Protein 16 ELISA Kit
Gene Names
IFI16; PYHIN2; IFNGIP1
Reactivity
Human
Pricing
Standard Curve (Sample)
defensin, alpha 1, ELISA Kit (Cat# AAA15540)
Full Name
Human defensin, alpha 1, DEFA1 ELISA Kit
Gene Names
DEFA1B; HP1; HP-1; HNP-1
Reactivity
Human
Pricing
RUNX2, Monoclonal Antibody (Cat# AAA24349)
Full Name
RUNX2 (Runt-related Transcription Factor 2, Core-binding Factor Subunit alpha-1, CBF-alpha-1, Acute Myeloid Leukemia 3 Protein, Oncogene AML-3, Polyomavirus Enhancer-binding Protein 2 alpha A Subunit, PEBP2-alpha A, PEA2-alpha A, SL3-3 Enhancer Factor 1 a
Gene Names
RUNX2; CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4: Alexa Fluor 488)
CD4, Monoclonal Antibody (Cat# AAA12065)
Full Name
RAT ANTI DOG CD4:RPE
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image:RPE conjugated Mouse anti Canine CD4 antibody, clone YKIC302.9used for the assessment of CD4 levels on canine cells by flow cytometry.Image caption:Immunophenotypic profile of tumor infiltrating lymphocyte in canine mammary carcinomas. Analysis of tumor infiltrating T-cells, B-lymphocytes and T-cell subsets from MC-BMT or MC (A), further subcategorized according to the absence (-) or presence (+) of lymph node metastasis (-) (B). Lymphocyte populations and subsets were identified by flow cytometric immunostaining as described in Material and Methods. Data were expressed as percentage of positive cells within gated lymphocytes and CD4+/CD8+ T-cell ratio. Significant differences at p < 0.05 are highlighted by asterisk.From: From: Estrela-Lima et al.BMC Cancer 2010 10:256.)
CD4, Monoclonal Antibody (Cat# AAA12279)
Full Name
RAT ANTI DOG CD4: RPE-Cy7
Applications
Flow Cytometry
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
Application Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE)
CD14, Monoclonal Antibody (Cat# AAA11884)
Full Name
MOUSE ANTI HUMAN CD14:FITC
Applications
Flow Cytometry
Pricing
Application Data
(Mouse TREM2 transfected cells stained with Rat anti Mouse TREM2 followed by Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed,))
TREM-2, Monoclonal Antibody (Cat# AAA12242)
Full Name
RAT ANTI MOUSE TREM-2:Low Endotoxin
Gene Names
Trem2; TREM-2; Trem2a; Trem2b; Trem2c
Applications
Flow Cytometry, Functional Assay
Pricing
Application Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE)
CD14, Monoclonal Antibody (Cat# AAA12178)
Full Name
MOUSE ANTI HUMAN CD14:APC
Applications
Flow Cytometry
Pricing
WB (Western Blot)
(Figure-1: Western blot analysis of MYADM. Anti-MYADM antibody (AAA14880) was used at 1 ug/ml on h Lung lysate.)
MYADM, Polyclonal Antibody (Cat# AAA14880)
Full Name
Polyclonal Antibody to MYADM
Gene Names
MYADM; SB135
Reactivity
Human
Applications
Western Blot
Purity
Purified
Protein A Chromatography
Protein A Chromatography
Pricing
WB (Western Blot)
(Gel: 12% SDS-PAGE Lysate: 40 μg Lane 1-2: Hepg2 and 293T cell Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds)
TREM1, Polyclonal Antibody (Cat# AAA29725)
Full Name
TREM1 Antibody
Gene Names
TREM1; CD354; TREM-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Pricing
Application Data
(PE conjugatedMouse anti Human CD169 antibody, clone 7-239 used to block CD169 function on myeloid cells.Image caption:Siglec-1 mediates HIV-1 uptake into a storage compartment and enhances HIV-1 trans-infection specially in IFN?-treated monocytes and DCs. A. Uptake of HIV-1NL4–3 by different myeloid cells exposed to IFN?. Cells were cultured with HIV-1 to measure p24Gag by ELISA. Mean values and SEM from four experiments include cells from 12 donors. B. Fold change in HIV-1NL4–3 uptake of cells treated with bafilomycin A1 compared to untreated cells. Mean values and SEM include cells from three donors. C. Relative uptake of HIV-1NL4–3 by IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 uptake by mock-treated cells (set at 100%). Mean values and SEM from two experiments include cells from six donors. D. Confocal microscopy analysis of different IFN?-treated myeloid cells pulsed with HIV-1Cherry and stained for Siglec-1 (Alexa 488), HLA-DR (Alexa 647) and DAPI. (Top) Representative viral pattern for each kind of myeloid cell analyzed, showing maximum fluorescence intensity of four channels. (Bottom) Percentage of myeloid cells with distinct viral patterns: random distribution, polarized accumulation, and sac-like compartment formation, as illustrated in the left drawing. Mean values of 50 cells from two different donors are shown. E. HIV-1 transmission from IFN?-treated myeloid cells to a luciferase reporter CD4+ cell line. HIV-1 infection was determined by induced luciferase activity in relative light units (RLUs). Mean values and SEM from four experiments include cells from 12 donors. F. Relative HIV-1 transmission from IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 trans-infected by mock-treated cells. Mean values and SEM from two experiments include cells from six donors. Statistical differences were assessed with a paired t test in A and E, and with a one sample t-test in B, C and F.From: Pino M, Erkizia I, Benet S, Erikson E, Fernández-Figueras MT, Guerrero D, Dalmau J, Ouchi D, Rausell A, Ciuffi A, Keppler OT, Telenti A, Kräusslich HG, Martinez-Picado J, Izquierdo-Useros N.HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells.Retrovirology. 2015 May 7;12:37.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
CD169, Monoclonal Antibody (Cat# AAA12264)
Full Name
Mouse Anti Human CD169
Gene Names
SIGLEC1; SN; CD169; SIGLEC-1
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Purity
>95% by SDS PAGE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
Application Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE)
CD14, Monoclonal Antibody (Cat# AAA12054)
Full Name
MOUSE ANTI HUMAN CD14:RPE
Applications
Flow Cytometry
Pricing
Application Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE)
CD14, Monoclonal Antibody (Cat# AAA11841)
Full Name
MOUSE ANTI HUMAN CD14:APC
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(Flow Cytometric Analysis of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).)
CD15/FUT4, Monoclonal Antibody (Cat# AAA13849)
Full Name
CD15/FUT4 (Reed-Sternberg Cell Marker)
Gene Names
FUT4; LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
Reactivity
Human, Mouse, Rat. Others not known.
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ICOS-L Mouse Monoclonal Antibody (ICOSL/3111). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
ICOS-L/ICOS Ligand/B7RP-1 (Immuno-Oncology Target), Monoclonal Antibody (Cat# AAA23916)
Full Name
ICOS-L/ICOS Ligand/B7RP-1 (Immuno-Oncology Target)
Gene Names
ICOSLG; B7h; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD40:RPE)
CD40, Monoclonal Antibody (Cat# AAA12090)
Full Name
MOUSE ANTI HUMAN CD40
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Anti- S100A9 Picoband antibody, AAA11645, IHC(P)IHC(P): Rat Spleen Tissue)
S100A9, Polyclonal Antibody (Cat# AAA11645)
Full Name
Anti-S100A9 Antibody
Gene Names
S100a9; p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen Affinity Purified
Pricing
Application Data
(AAA31372 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31372 1:200) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
RUNX2, Polyclonal Antibody (Cat# AAA31372)
Full Name
Phospho-RUNX2 (S340) Antibody
Gene Names
RUNX2; CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD40:RPE)
CD40, Monoclonal Antibody (Cat# AAA11867)
Full Name
MOUSE ANTI HUMAN CD40:FITC
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
Flow Cytometry
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD40:RPE)
CD40, Monoclonal Antibody (Cat# AAA11936)
Full Name
MOUSE ANTI HUMAN CD40
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Reactivity
Dog
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD40:RPE)
CD40, Monoclonal Antibody (Cat# AAA12089)
Full Name
MOUSE ANTI HUMAN CD40:RPE
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
Flow Cytometry
Pricing
IHC (Immunohistchemistry-Paraffin)
(AAA31234 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
KMT2A/MLL, Polyclonal Antibody (Cat# AAA31234)
Full Name
KMT2A/MLL Antibody
Gene Names
KMT2A; HRX; MLL; MLL1; TRX1; ALL-1; CXXC7; HTRX1; MLL1A; WDSTS
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(100%), Dog(100%), Chicken(88%)
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(100%), Dog(100%), Chicken(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
Application Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE)
CD14, Monoclonal Antibody (Cat# AAA11997)
Full Name
MOUSE ANTI HUMAN CD14
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD40:RPE)
CD40, Monoclonal Antibody (Cat# AAA12031)
Full Name
MOUSE ANTI HUMAN CD40:RPE
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
Flow Cytometry
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31201 at 1/100 staining Human lung cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
PICALM, Polyclonal Antibody (Cat# AAA31201)
Full Name
PICALM Antibody
Gene Names
PICALM; LAP; CALM; CLTH
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of PFA-fixed Raji cells. CD79a Mouse Monoclonal Antibody (JCB117) followed by goat anti-mouse IgG-CF488. Nuclei counterstained with RedDot.)
CD79a, Monoclonal Antibody (Cat# AAA23891)
Full Name
CD79a (B-Cell Marker) Mouse Monoclonal Antibody
Gene Names
CD79A; IGA; MB-1
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
IHC (Immunohistochemistry)
(AAA31079 at 1/200 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
MCL1, Polyclonal Antibody (Cat# AAA31079)
Full Name
MCL1 Antibody
Gene Names
MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
Application Data
(Mouse anti Human CD14 antibody, clone Tük4 used to identify bovine monocytes in the skin and subcutaneous tissue of adjuvant injected calves by immunofluorescence.Image caption:Cellular recruitment to skin and subcutaneous tissues. (A) HE stained sections of skin with subcutaneous tissue from the side injected with adjuvant and the contralateral side, at 24 h post-injection. Scale bars: 200 ?m. (B) Enlargement of outlined areas in A, as indicated. Scale bars: 20 ?m. (C) Immunofluorescent labeling of subcutaneous tissue on the injected side with antibody against CD14 (green). Scale bar: 20 ?m.From: Lund H, Boysen P, Åkesson CP, Lewandowska-Sabat AM and Storset AK (2016)Transient Migration of Large Numbers of CD14++ CD16+ Monocytes to the Draining Lymph Node after Onset of Inflammation.Front. Immunol. 7:322.This is from an open access article distributed under the terms of the Creative Commons Attribution License.)
CD14, Monoclonal Antibody (Cat# AAA12266)
Full Name
Mouse Anti Human CD14: Amethyst Orange
Reactivity
Human
Applications
Flow Cytometry
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
Application Data
(PE conjugatedMouse anti Human CD169 antibody, clone 7-239 used to block CD169 function on myeloid cells.Image caption:Siglec-1 mediates HIV-1 uptake into a storage compartment and enhances HIV-1 trans-infection specially in IFN?-treated monocytes and DCs. A. Uptake of HIV-1NL4–3 by different myeloid cells exposed to IFN?. Cells were cultured with HIV-1 to measure p24Gag by ELISA. Mean values and SEM from four experiments include cells from 12 donors. B. Fold change in HIV-1NL4–3 uptake of cells treated with bafilomycin A1 compared to untreated cells. Mean values and SEM include cells from three donors. C. Relative uptake of HIV-1NL4–3 by IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 uptake by mock-treated cells (set at 100%). Mean values and SEM from two experiments include cells from six donors. D. Confocal microscopy analysis of different IFN?-treated myeloid cells pulsed with HIV-1Cherry and stained for Siglec-1 (Alexa 488), HLA-DR (Alexa 647) and DAPI. (Top) Representative viral pattern for each kind of myeloid cell analyzed, showing maximum fluorescence intensity of four channels. (Bottom) Percentage of myeloid cells with distinct viral patterns: random distribution, polarized accumulation, and sac-like compartment formation, as illustrated in the left drawing. Mean values of 50 cells from two different donors are shown. E. HIV-1 transmission from IFN?-treated myeloid cells to a luciferase reporter CD4+ cell line. HIV-1 infection was determined by induced luciferase activity in relative light units (RLUs). Mean values and SEM from four experiments include cells from 12 donors. F. Relative HIV-1 transmission from IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 trans-infected by mock-treated cells. Mean values and SEM from two experiments include cells from six donors. Statistical differences were assessed with a paired t test in A and E, and with a one sample t-test in B, C and F.From: Pino M, Erkizia I, Benet S, Erikson E, Fernández-Figueras MT, Guerrero D, Dalmau J, Ouchi D, Rausell A, Ciuffi A, Keppler OT, Telenti A, Kräusslich HG, Martinez-Picado J, Izquierdo-Useros N.HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells.Retrovirology. 2015 May 7;12:37.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
CD169, Monoclonal Antibody (Cat# AAA12265)
Full Name
Mouse Anti Human CD169: RPE
Gene Names
SIGLEC1; SN; CD169; SIGLEC-1
Reactivity
Human
Applications
Flow Cytometry
Purity
>95% by SDS PAGE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
Application Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . Low power)
CD163, Monoclonal Antibody (Cat# AAA11943)
Full Name
MOUSE ANTI HUMAN CD163
Gene Names
CD163; M130; MM130
Reactivity
Guinea Pig, Pig, Rhesus Monkey, Sheep
Applications
Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoassay, Western Blot
Pricing
Application Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE)
CD14, Monoclonal Antibody (Cat# AAA12179)
Full Name
MOUSE ANTI HUMAN CD14
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
IF (Immunofluorescence)
(AAA31227 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31227 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
SET, Polyclonal Antibody (Cat# AAA31227)
Full Name
SET Antibody
Gene Names
SET; 2PP2A; IGAAD; MRD58; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA
Reactivity
Human, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
Application Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE)
CD14, Monoclonal Antibody (Cat# AAA11998)
Full Name
MOUSE ANTI HUMAN CD14
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
WB (Western Blot)
(Western blot analysis of AFX phosphorylation expression in serum treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
AFX, Polyclonal Antibody (Cat# AAA31062)
Full Name
Phospho-AFX (Ser197) Antibody
Gene Names
FOXO4; AFX; AFX1; MLLT7
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of FOXO4 phosphorylation expression in EGF treated HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
FOXO4, Polyclonal Antibody (Cat# AAA31061)
Full Name
Phospho-FOXO4 (Thr451) Antibody
Gene Names
FOXO4; AFX; AFX1; MLLT7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Flow Cytometric Analysis of Human Raji cells using HLA-DP (MHC II) Monoclonal Antibody (SPM421) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).)
HLA-DP, Antibody (Cat# AAA13830)
Full Name
HLA-DP (MHC II) Mouse Monoclonal Antibody
Gene Names
HLA-DPB1; DPB1; HLA-DP; HLA-DPB; HLA-DP1B
Reactivity
Human. Others not known.
Applications
Flow Cytometry, Immunofluorescence
Pricing
FCM (Flow Cytometry)
(Flow Cytometric Analysis of PFA-fixed Raji cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).)
Human Nuclear Antigen, Monoclonal Antibody (Cat# AAA13804)
Full Name
Human Nuclear Antigen
Reactivity
Human and Non-human primates. Does not react with mouse, rat, and chicken. Others not known.
Applications
Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry
Pricing
SDS-PAGE
S100A8, Recombinant Protein (Cat# AAA11783)
Full Name
S100A8, 1- 93aa, Human, E Coli
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody)
MYD88, Polyclonal Antibody (Cat# AAA13652)
Full Name
Goat anti-MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Peptide ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
Application Data
(Staining of human peripheral blood monocytes probed with Mouse anti Human CD14:FITC)
CD14, Monoclonal Antibody (Cat# AAA12224)
Full Name
MOUSE ANTI HUMAN CD14:Biotin
Applications
Flow Cytometry
Pricing