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Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
683 results for " D" - showing 100-150
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
H2B, Polyclonal Antibody (Cat# AAA31348)
Full Name
Acetyl-H2B (Lys5) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Standard Curve (Sample)
Beta-Galactosidase, Assay Kit (Cat# AAA27852)
Full Name
Beta-Galactosidase Microplate Assay Kit
Gene Names
GLB1; EBP; ELNR1; MPS4B
Reactivity
General
Applications
Functional Assay
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse stomach using CHRFAM7A antibody at dilution of 1:100 (40x lens).)
CHRFAM7A, Polyclonal Antibody (Cat# AAA28208)
Full Name
CHRFAM7A Polyclonal Antibody
Gene Names
CHRFAM7A; D-10; CHRNA7; CHRNA7-DR1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Cytokeratin 18 was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- Cytokeratin 18 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
Cytokeratin 18, Polyclonal Antibody (Cat# AAA11682)
Full Name
Anti-Cytokeratin 18 Antibody
Gene Names
KRT18; K18; CK-18; CYK18
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12162)
Full Name
RAT ANTI MOUSE F4/80:Biotin
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12161)
Full Name
RAT ANTI MOUSE F4/80:APC
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12172)
Full Name
RAT ANTI MOUSE F4/80:RPE
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry, Immunofluorescence
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12217)
Full Name
RAT ANTI MOUSE F4/80:APC
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p)
CD68, Monoclonal Antibody (Cat# AAA12105)
Full Name
RAT ANTI MOUSE CD68:FITC
Gene Names
Cd68; Lamp4; gp110; Scard1
Applications
Flow Cytometry
Pricing
Standard Curve (Sample)
platelet derived growth factor DD, ELISA Kit (Cat# AAA18173)
Full Name
Rat Platelet-derived growth factor DD, PDGF-DD ELISA Kit
Reactivity
Rat
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H2B, Polyclonal Antibody (Cat# AAA31351)
Full Name
Phospho-Histone H2B (Tyr43) Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA11971)
Full Name
MOUSE ANTI RAT CD11b
Gene Names
ITGAM; CD11B
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-GBA antibody. Counter stained with hematoxylin.)
GBA, Monoclonal Antibody (Cat# AAA30312)
Full Name
GBA Antibody
Gene Names
GBA; GCB; GBA1; GLUC
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
ProA affinity purified
Pricing
IHC (Immunohistchemistry)
((3.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
KCNN4/KCa3.1, Antibody (Cat# AAA13678)
Full Name
Goat Anti-KCNN4/KCa3.1 Antibody
Gene Names
KCNN4; IK; IK1; SK4; DHS2; KCA4; hSK4; IKCA1; hKCa4; KCa3.1; hIKCa1
Reactivity
Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog
Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Peptide ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
(Anti-HA Tag mouse monoclonal antibody at 1:1000 dilutionLane A: HA-GST (Recombinant protein)(30ng)Lane B: GST-HA (Recombinant protein)(10ng)Lane C: HA-ARG1-myc transfected 293 cell lysate (2ug)Lane D: HA-mFABP4-myc transfected 293 cell lysate (2ug)Lane E: myc-mFABP4-HA transfected 293 cell lysate (0.5ug)Lane F: myc-ARG1-HA transfected 293 cell lysate (2ug)SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)
HA, Monoclonal Antibody (Cat# AAA27712)
Full Name
Anti-HA tag Antibody, Mouse Monoclonal
Reactivity
HA tag
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H2A, Polyclonal Antibody (Cat# AAA31347)
Full Name
Acetyl-Histone H2A (Lys5) Antibody
Gene Names
HIST1H2AB; H2A/m; H2AFM
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
ICC (Immunocytochemistry)
(ICC staining Histone H3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Histone H3, Monoclonal Antibody (Cat# AAA30242)
Full Name
Histone H3 Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Chromatin Immunoprecipitation
Purity
ProA affinity purified
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation analysis extracts of 293 cell line, using TriMethyl-Histone H3-K4 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
H3K4me3, Antibody (Cat# AAA10662)
Full Name
Histone H3K4me3 Polyclonal Antibody
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
WB (Western Blot)
(SMARCD2 monoclonal antibody (M02), clone 2B2. Western Blot analysis of SMARCD2 expression in Raw 264.7.)
SMARCD2, Monoclonal Antibody (Cat# AAA25973)
Full Name
SMARCD2 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of chromatin, Subfamily d, Member 2, BAF60B, CRACD2, PRO2451, Rsc6p) (AP)
Gene Names
SMARCD2; SGD2; Rsc6p; BAF60B; CRACD2; PRO2451
Applications
Western Blot
Purity
Purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with TCF7L2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
TCF7L2, Monoclonal Antibody (Cat# AAA30140)
Full Name
TCF7L2 Antibody
Gene Names
TCF7L2; TCF4; TCF-4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
WB (Western Blot)
(SMARCD2 monoclonal antibody (M02), clone 2B2. Western Blot analysis of SMARCD2 expression in Raw 264.7.)
SMARCD2, Monoclonal Antibody (Cat# AAA26372)
Full Name
SMARCD2 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of chromatin, Subfamily d, Member 2, BAF60B, CRACD2, PRO2451, Rsc6p) (FITC)
Gene Names
SMARCD2; SGD2; Rsc6p; BAF60B; CRACD2; PRO2451
Applications
Immunofluorescence, Western Blot
Purity
Purified
Pricing
WB (Western Blot)
(SMARCD2 monoclonal antibody (M02), clone 2B2. Western Blot analysis of SMARCD2 expression in Raw 264.7.)
SMARCD2, Monoclonal Antibody (Cat# AAA26239)
Full Name
SMARCD2 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of chromatin, Subfamily d, Member 2, BAF60B, CRACD2, PRO2451, Rsc6p) (Biotin)
Gene Names
SMARCD2; SGD2; Rsc6p; BAF60B; CRACD2; PRO2451
Applications
Immunofluorescence, Western Blot
Purity
Purified
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of C2C12 cells, either LY294002-treated (left) or insulin-treated (right), using AAA14695 (green). Actin filaments have been labeled with DY554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)
Akt, pan, Monoclonal Antibody (Cat# AAA14695)
Full Name
Akt, pan (Rac PKa, PKBa)
Gene Names
Akt1; akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq;
Reactivity
Human, Monkey, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Immunofluorescence
Purity
Ascites
Ascites
Ascites
Pricing
Application Data
(Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p)
CD68, Monoclonal Antibody (Cat# AAA12103)
Full Name
RAT ANTI MOUSE CD68:Biotin
Gene Names
Cd68; Lamp4; gp110; Scard1
Applications
Flow Cytometry
Pricing
Application Data
(Detection limit for recombinant GST tagged PEPD is ~0.1ng/ml as a capture antibody.)
PEPD, Monoclonal Antibody (Cat# AAA25196)
Full Name
PEPD (Xaa-Pro Dipeptidase, X-Pro Dipeptidase, Proline Dipeptidase, Prolidase, Imidodipeptidase, Peptidase D, PRD, MGC10905) (FITC)
Gene Names
PEPD; PROLIDASE
Reactivity
Human
Applications
Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Published customer imageSLAMF3 blockade in human hepatocytes is associated with lower susceptibility to HCV. (A) SLAMF3 was stained in primary human hepatocytes (PHHs) and cells from the Huh-7 human hepatoma cell line with a specific antibody (HLy9.1.25 clone; grey) and an isotype-matched control (empty). One of four independent experiments is shown. Huh-7 cells were transfected with scrambled control (sc) siRNA or three specific siRNAs (#1, #2 and #3) targeting SLAMF3, prior to infection with HCVcc; siRNA efficiency was checked by quantifying SLAMF3 mRNA (B) and the CD81 expression level (C) by flow cytometry analysis at 48 h post-transfection. Results are presented as the mean +/-SD (n = 3). Intracellular viral RNA was quantified at 72 h p.i. (D) and the infection was measured at 72 h p.i. by focus-forming units FFUs counting (E) (as inhibition percent; mean of three independent experiments; error bars: SD. **p)
CD229, Monoclonal Antibody (Cat# AAA11951)
Full Name
MOUSE ANTI HUMAN CD229
Gene Names
LY9; hly9; mLY9; CD229; SLAMF3
Applications
Flow Cytometry, Immunoprecipitation
Pricing
Standard Curve (Sample)
25-hydroxy vitamin D (25 (OH) D), ELISA Kit (Cat# AAA12830)
Full Name
Chicken 25-hydroxy vitamin D (25 (OH) D) ELISA Kit
Reactivity
Chicken
Pricing
Application Data
(Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p)
CD68, Monoclonal Antibody (Cat# AAA12110)
Full Name
RAT ANTI MOUSE CD68
Gene Names
Cd68; Lamp4; gp110; Scard1
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
PKC delta, Polyclonal Antibody (Cat# AAA31409)
Full Name
Phospho-PKC delta (Ser664) Antibody
Gene Names
PRKCD; MAY1; PKCD; nPKC-delta
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (82%), Bovine (91%), Horse (91%), Sheep (91%), Dog (91%), Chicken (91%), Xenopus (82%)
Predicted Reactivity: Pig (82%), Bovine (91%), Horse (91%), Sheep (91%), Dog (91%), Chicken (91%), Xenopus (82%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Medium power)
CD314, Monoclonal Antibody (Cat# AAA11960)
Full Name
MOUSE ANTI HUMAN CD314
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
WB (Western Blot)
(Anti-ANP32A rabbit polyclonal antibody at 1:500 dilution Lane A: ANP32A konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa)
ANP32A, Polyclonal Antibody (Cat# AAA27748)
Full Name
Anti-ANP32A Antibody, Rabbit Polyclonal
Gene Names
ANP32A; LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
Application Data
(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta5 antibody followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power))
ESTROGEN RECEPTOR BETA 5, Monoclonal Antibody (Cat# AAA12216)
Full Name
MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 5
Gene Names
ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12185)
Full Name
RAT ANTI MOUSE CD11b
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12183)
Full Name
RAT ANTI MOUSE CD11b:FITC
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
Application Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . Low power)
CD163, Monoclonal Antibody (Cat# AAA12100)
Full Name
MOUSE ANTI HUMAN CD163
Gene Names
CD163; M130; MM130
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoassay, Immunohistochemistry, Western Blot
Pricing
IHC (Immunohistochemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA29653 (DRD1 Antibody) at dilution 1/50, on the right is treated with the synthetic peptide.)
DRD1, Polyclonal Antibody (Cat# AAA29653)
Full Name
DRD1 Antibody
Gene Names
DRD1; DADR; DRD1A
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
Application Data
(Detection limit for recombinant GST tagged PEPD is ~0.1ng/ml as a capture antibody.)
PEPD, Monoclonal Antibody (Cat# AAA24604)
Full Name
PEPD (Xaa-Pro Dipeptidase, X-Pro Dipeptidase, Proline Dipeptidase, Prolidase, Imidodipeptidase, Peptidase D, PRD, MGC10905) APC
Gene Names
PEPD; PROLIDASE
Reactivity
Human
Applications
Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
MARK2, Polyclonal Antibody (Cat# AAA31392)
Full Name
Phospho-MARK2 (Thr596) Antibody
Gene Names
MARK2; EMK1; EMK-1; PAR-1; Par1b; Par-1b
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
CD11b, Monoclonal Antibody (Cat# AAA12187)
Full Name
RAT ANTI MOUSE CD11b:Biotin
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA11876)
Full Name
MOUSE ANTI RAT CD11b:FITC
Gene Names
ITGAM; CD11B
Applications
Flow Cytometry
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12169)
Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot
Pricing
IHC (Immunohistochemistry)
(AAA30929 at 1/200 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Histone H3, Polyclonal Antibody (Cat# AAA30929)
Full Name
Histone H3 Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Histone H3, Polyclonal Antibody (Cat# AAA31322)
Full Name
Phospho-Histone H3 (Thr3) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Detection limit for recombinant GST tagged PCMT1 is ~0.1ng/ml as a capture antibody.)
PCMT1, Monoclonal Antibody (Cat# AAA25486)
Full Name
PCMT1 (Protein-L-isoaspartate(D-aspartate) O-methyltransferase, PIMT, Protein-beta-Aspartate Methyltransferase, Protein L-isoaspartyl/D-aspartyl Methyltransferase, L-isoaspartyl Protein Carboxyl Methyltransferase) (HRP)
Gene Names
PCMT1; PIMT
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells with Cytokeratin 18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Cytokeratin 18, Monoclonal Antibody (Cat# AAA30013)
Full Name
Cytokeratin 18 Antibody
Gene Names
KRT18; K18; CYK18
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12163)
Full Name
RAT ANTI MOUSE F4/80:Biotin
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
Flow Cytometry
Pricing
Standard Curve (Sample)
Lipopolysaccharide Binding Protein (LBP), ELISA Kit (Cat# AAA13045)
Full Name
Human Lipopolysaccharide Binding Protein (LBP) ELISA Kit
Gene Names
LBP; BPIFD2
Reactivity
Human
Pricing
Standard Curve (Sample)
1,3-Beta-D-glucan (BDG), ELISA Kit (Cat# AAA17296)
Full Name
Human 1,3-Beta-D-glucan (BDG) ELISA Kit
Reactivity
Human
Pricing
WB (Western Blot)
(Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Daudi Whole Cell Lysate Lane C: MOLT-4 Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lane E: 293T Whole Cell Lysate Lane F: HelaS3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)
Beta-Tubulin, Monoclonal Antibody (Cat# AAA27713)
Full Name
Beta-Tubulin Loading Control Antibody, Mouse MAb
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A
Pricing
SDS-PAGE
(SDS-PAGE Analysis Purified HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1). Confirmation of Purity and Integrity of Antibody.)
HSP60 (Heat Shock Protein 60), Monoclonal Antibody (Cat# AAA23919)
Full Name
HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing