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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
1420 results for " Primary IHC" - showing 1350-1400
FCM (Flow Cytometry)
(Overlay histogram showing U87 cells stained with AAA28063 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
CD9, Monoclonal Antibody (Cat# AAA28063)
Full Name
CD9 Monoclonal Antibody
Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
>95%, Protein G purified
Pricing
IF (Immunofluorescence)
(AAA30990 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Lyn, Polyclonal Antibody (Cat# AAA30990)
Full Name
Phospho-Lyn (Tyr508) Antibody
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30967 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Lamin A/C, Polyclonal Antibody (Cat# AAA30967)
Full Name
Phospho-Lamin A/C (Ser392) Antibody
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30924 staining MCF-7 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Ki67, Polyclonal Antibody (Cat# AAA30924)
Full Name
Ki67 Antibody
Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientifiv).
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H3, Polyclonal Antibody (Cat# AAA31353)
Full Name
Acetyl-Histone H3 (Lys122) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%)
Predicted Reactivity: Bovine (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistochemistry)
Calponin, Polyclonal Antibody (Cat# AAA14404)
Full Name
Calponin Antibody
Gene Names
CNN1; SMCC; Sm-Calp; HEL-S-14
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/25.)
CD63, Polyclonal Antibody (Cat# AAA29720)
Full Name
CD63 Antibody
Gene Names
CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min))
MET, Polyclonal Antibody (Cat# AAA29724)
Full Name
MET Antibody
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antigen Affinity Purification
Pricing
IHC (Immunohistochemistry)
(IHC of Mouse kidney using Z01 antibody and FFPE tissue after heat-induced retrieval. Anti-Z01 Ab at 1:750/DAB detection.)
ZO-1, Polyclonal Antibody (Cat# AAA13861)
Full Name
ZO-1 Polyclonal Antibody
Gene Names
Tjp1; ZO1
Reactivity
Reacts against Human, Rat, Mouse, Canine and Monkey proteins.
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Epitope-affinity purified from goat antiserum
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Ryanodine Receptor 2 (phospho-Ser2808) staining in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Ryanodine Receptor 2 (phospho-Ser2808), Polyclonal Antibody (Cat# AAA29761)
Full Name
Ryanodine Receptor 2 (phospho-Ser2808) Antibody
Gene Names
RYR2; RyR; ARVC2; ARVD2; RYR-2; VTSIP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Immunogen affinity chromatography
Pricing
WB (Western Blot)
(CHAK1 Antibody (AAA28787) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHAK1 antibody detected the CHAK1 protein (arrow).)
TRPM7 (CHAK1), Polyclonal Antibody (Cat# AAA28787)
Full Name
TRPM7 (CHAK1) Antibody (C-term)
Gene Names
TRPM7; CHAK; CHAK1; ALSPDC; LTRPC7; LTrpC-7; TRP-PLIK
Reactivity
Human, Mouse
Predicted: Rat
Predicted: Rat
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IS (Immunostaining)
(Immunostaining of 25?m thick cryosections of PFA-perfused Mouse Amygdala, antigen retrieval withcitrate buffer Ph 6 at 80C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.)
Androgen Receptor, Polyclonal Antibody (Cat# AAA13656)
Full Name
Goat anti-Androgen Receptor Antibody
Gene Names
AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
Peptide ELISA, Western Blot, Immunohistochemistry, Chromatin Immunoprecipitation
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistochemistry)
(AAA31055 at 1/200 staining Mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
LIMK1, Polyclonal Antibody (Cat# AAA31055)
Full Name
Phospho-LIMK1 (Thr508) Antibody
Gene Names
LIMK1; LIMK; LIMK-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MMP12 Antibody (C-term) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
MMP12, Polyclonal Antibody (Cat# AAA28713)
Full Name
MMP12 Antibody (C-term)
Gene Names
MMP12; ME; HME; MME; MMP-12
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(AAA30994 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IGF1R, Polyclonal Antibody (Cat# AAA30994)
Full Name
Phospho-IGF1R (Tyr1161) Antibody
Gene Names
IGF1R; IGFR; CD221; IGFIR; JTK13
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30959 at 1/100 staining rat testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk2, Polyclonal Antibody (Cat# AAA30959)
Full Name
Phospho-Chk2 (Ser516) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30991 at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary Ab.)
SP1, Polyclonal Antibody (Cat# AAA30991)
Full Name
Phospho-SP1 (Thr453) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-EGFR (Ser1071) expression in various lysates)
EGFR, Polyclonal Antibody (Cat# AAA30964)
Full Name
Phospho-EGFR (Ser1071) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(All lanes : Anti-LAG3 Antibody (Center) at 1:1000 dilutionLane 1: human ovary lysateLane 2: mouse liver lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 57 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
LAG3, Polyclonal Antibody (Cat# AAA28766)
Full Name
LAG3 Antibody (Center)
Gene Names
LAG3; CD223
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(AAA30935 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
GR, Polyclonal Antibody (Cat# AAA30935)
Full Name
Phospho-GR (Ser211) Antibody
Gene Names
NR3C1; GR; GCR; GRL; GCCR; GCRST
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot of BAFF/BLyS in mouse and human cell lysates. Lane A: mouse testis, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human).)
BAFF, Polyclonal Antibody (Cat# AAA12314)
Full Name
Rabbit Polyclonal (IgG) to Human BAFF
Gene Names
TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Protein G Purified
Pricing
WB (Western Blot)
(Western blot analysis of STAT3 phosphorylation expression in HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
STAT3, Polyclonal Antibody (Cat# AAA31042)
Full Name
Phospho-STAT3 (Ser727) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cell using MMP16 antibody (AAA28228) at dilution of 1:100. Blue: DAPI for nuclear staining)
MMP16, Polyclonal Antibody (Cat# AAA28228)
Full Name
MMP16 Polyclonal Antibody
Gene Names
MMP16; MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(AAA13655 Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody.)
CCKBR, Polyclonal Antibody (Cat# AAA13655)
Full Name
Goat anti-CCKBR Antibody
Gene Names
CCKBR; GASR; CCK-B; CCK2R
Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow
Expected from sequence similarity: Human, Mouse, Rat, Cow
Applications
Peptide ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-Tyrosine Hydroxylase (Ser19) expression in various lysates)
Tyrosine Hydroxylase, Polyclonal Antibody (Cat# AAA30988)
Full Name
Phospho-Tyrosine Hydroxylase (Ser19) Antibody
Gene Names
TH; TYH; DYT14; DYT5b
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30944 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
HSP20, Polyclonal Antibody (Cat# AAA30944)
Full Name
Phospho-HSP20 (Ser16) Antibody
Gene Names
HSPB6; HEL55; Hsp20; PPP1R91
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30932 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA30932 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
TGF beta1, Polyclonal Antibody (Cat# AAA30932)
Full Name
TGF beta1 Antibody
Gene Names
TGFB1; CED; LAP; DPD1; TGFB; TGFbeta
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry)
(Phospho-ERK1/2 (Thr202/Tyr204) Antibody for IHC in human testis)
ERK1/2, Polyclonal Antibody (Cat# AAA30930)
Full Name
Phospho-ERK1/2 (Thr202/Tyr204) Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30946 at 1/100 staining rat cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk1, Polyclonal Antibody (Cat# AAA30946)
Full Name
Phospho-Chk1 (Ser286) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30940 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
GSK3 beta, Polyclonal Antibody (Cat# AAA30940)
Full Name
Phospho-GSK3 beta (Ser9) Antibody
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
Peptide affinity purification
Pricing
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with ACE2 (SARS Receptor) Antibody (C-term) (AAA28762), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
ACE2 (SARS Receptor), Polyclonal Antibody (Cat# AAA28762)
Full Name
ACE2 (SARS Receptor) Antibody (C-term)
Gene Names
ACE2; ACEH
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of methanol-fixed HeLa, using Histamine H2 Receptor antibody at 1: 200 dilution.)
Histamine H2 Receptor, Polyclonal Antibody (Cat# AAA29716)
Full Name
Histamine H2 Receptor antibody
Gene Names
HRH2; H2R
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified by antigen-affinity chromatography.
Pricing
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Sample: Human Cerebrum TissuePrimary Ab: 20ug/ml Mouse Anti-HumanAQP4 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked CaprineAnti-Mouse IgG Polyclonal Antibody (Immunohistochemistry))
Aquaporin 4 (AQP4), Monoclonal Antibody (Cat# AAA21051)
Full Name
Monoclonal Antibody to Aquaporin 4 (AQP4)
Gene Names
AQP4; MIWC; WCH4
Reactivity
Human, Rat
Applications
Westen Blot, Immunohistochemistry
Purity
Purification: Protein A + Protein G affinity chromatography
Pricing
FCM (Flow Cytometry)
(AAA13649 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Arrestin beta 2, Antibody (Cat# AAA13649)
Full Name
Goat anti-Arrestin beta 2 Antibody
Gene Names
ARRB2; ARB2; ARR2; BARR2
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with mouse Prodh Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
PRODH, Polyclonal Antibody (Cat# AAA28717)
Full Name
PRODH Antibody (Center)
Gene Names
Prodh; Pro1; Pro-1; Ym24d07
Reactivity
Human, mouse
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western blot analysis of extracts from rat brain, using MMP14 Ab.)
MMP14, Polyclonal Antibody (Cat# AAA30926)
Full Name
MMP14 Antibody
Gene Names
MMP14; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(AAA30949 at 1/50 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IKK alpha, Polyclonal Antibody (Cat# AAA30949)
Full Name
Phospho-IKK alpha (Ser176)/IKK beta (Ser177) Antibody
Gene Names
CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30965 at 1/100 staining mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Myc, Polyclonal Antibody (Cat# AAA30965)
Full Name
Phospho-Myc (Ser62) Antibody
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Bovine Cardiac Muscle Tissue;Primary Ab: 20ug/ml Rabbit Anti-Bovine CSN3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Casein Kappa (CSN3), Polyclonal Antibody (Cat# AAA21054)
Full Name
Polyclonal Antibody to Casein Kappa (CSN3)
Gene Names
CSN3; CSNK; CSN10; CSN3K
Reactivity
Human, Bovine
Applications
Westen Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
WB (Western Blot)
(Human Brain and cell HomogenatesDilution : 1:1000/ 1:2000)
APOE, Polyclonal Antibody (Cat# AAA23552)
Full Name
APOE antibody - N-terminal region
Gene Names
APOE; AD2; LPG; APO-E; ApoE4; LDLCQ5
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody)
MYD88, Polyclonal Antibody (Cat# AAA13652)
Full Name
Goat anti-MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Peptide ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing HeLa cells stained with AAA28782(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28782, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
HNRNPR, Polyclonal Antibody (Cat# AAA28782)
Full Name
HNRNPR Antibody (N-term)
Gene Names
HNRNPR; HNRPR; hnRNP-R
Reactivity
Human
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Glutathione S Transferase Mu 4, Polyclonal Antibody (Cat# AAA21016)
Full Name
Glutathione S Transferase Mu 4 (GSTm4) Polyclonal Antibody
Gene Names
Gstm7; Gstm3
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
Affinity Chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix™ coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
AIF1/IBA1, Polyclonal Antibody (Cat# AAA13659)
Full Name
Goat anti-AIF1/IBA1 (isoform 3) Antibody
Gene Names
AIF1; IBA1; IRT1; AIF-1; IRT-1
Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog
Expected from sequence similarity: Human, Mouse, Dog
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
Application Data
(Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.)
SLC26A3, Polyclonal Antibody (Cat# AAA23387)
Full Name
SLC26A3 antibody - middle region
Gene Names
SLC26A3; CLD; DRA
Reactivity
Tested Reactivity: Human
Predicted Reactivity: Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep
Predicted Reactivity: Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Pricing
Knockout Validation
(Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: CALML3 knockout A431 cell lysate; Predicted MW: 16kd Observed MW: 16kd Primary Ab: 1ug/ml Rabbit Anti-Human CALML3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Human.)
Calmodulin Like Protein 3, Polyclonal Antibody (Cat# AAA21024)
Full Name
Calmodulin Like Protein 3 (CALML3) Polyclonal Antibody
Gene Names
CALML3; CLP
Reactivity
Human, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
Affinity Chromatography
Pricing
IHC (Immunohistchemistry)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Kininogen 1, Polyclonal Antibody (Cat# AAA21045)
Full Name
Polyclonal Antibody to Kininogen 1 (KNG1)
Gene Names
Kng2l1; Kng1; Kng2; Kngk; KINKG; KINKH; kininogen
Reactivity
Rat, Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
Affinity Chromatography
Pricing
IHC (Immunohistochemistry)
(AAA30943 at 1/100 staining rat testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
I kappaB epsilon, Polyclonal Antibody (Cat# AAA30943)
Full Name
Phospho-I kappaB epsilon (Ser22) Antibody
Gene Names
NFKBIE; IKBE
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from K562 cells(H2O2 treatment), using Phospho-EGFR (Tyr992)[Tyr1016] Ab. The lane on the left was treated with blocking peptide.)
EGFR, Polyclonal Antibody (Cat# AAA30961)
Full Name
Phospho-EGFR (Tyr1016) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Knockout Validation
(Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: SMN2 knockout 293T cell lysate; Predicted MW: 32,30,29,27kd Observed MW: 37kd Primary Ab: 1ug/ml Rabbit Anti-Human SMN2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Survival Of Motor Neuron 2, Polyclonal Antibody (Cat# AAA21031)
Full Name
Survival Of Motor Neuron 2, Centromeric (SMN2) Polyclonal Antibody
Gene Names
SMN1; SMA; SMN; SMA1; SMA2; SMA3; SMA4; SMA@; SMNT; BCD541; GEMIN1; TDRD16A; T-BCD541
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
Affinity Chromatography
Pricing