Search results for " Membrane Protein"

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (AAA11670).Lipocalin 2 was detected in frozen section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Lipocalin 2 Antibody (AAA11670) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Lipocalin 2, Polyclonal Antibody (Cat# AAA11670)

Full Name

Anti-Lipocalin 2 Antibody

Gene Names

Lcn2; 24p3; Sip24; AW212229

Reactivity

Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen Affinity Purified

Pricing

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IF (Immunofluorescence) (IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-Band 3 Antibody overnight at 4 degree C. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

Band 3, Polyclonal Antibody (Cat# AAA11648)

Full Name

Anti-Band 3 Antibody

Gene Names

SLC4A1; DI; FR; SW; WD; WR; AE1; CHC; SAO; WD1; BND3; EPB3; SPH4; CD233; EMPB3; RTA1A

Reactivity

Human, Mouse , Rat.

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen Affinity Purified

Pricing

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IF (Immunofluorescence) (AAA30932 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA30932 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)

TGF beta1, Polyclonal Antibody (Cat# AAA30932)

Full Name

TGF beta1 Antibody

Gene Names

TGFB1; CED; LAP; DPD1; TGFB; TGFbeta

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Pricing

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GSK3 beta, Polyclonal Antibody (Cat# AAA30940)

Full Name

Phospho-GSK3 beta (Ser9) Antibody

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation

Purity

Peptide affinity purification

Pricing

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IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human testis tissue reacted with ACE2 (SARS Receptor) Antibody (C-term) (AAA28762), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

ACE2 (SARS Receptor), Polyclonal Antibody (Cat# AAA28762)

Full Name

ACE2 (SARS Receptor) Antibody (C-term)

Gene Names

ACE2; ACEH

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

Varicella Zoster Virus Glycoprotein E, Recombinant Protein (Cat# AAA14424)

Full Name

Varicella Zoster Virus Glycoprotein E Recombinant

Applications

ELISA

Purity

>90% by SDS-PAGE.

Pricing

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FCM (Flow Cytometry) (AAA13649 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)

Arrestin beta 2, Antibody (Cat# AAA13649)

Full Name

Goat anti-Arrestin beta 2 Antibody

Gene Names

ARRB2; ARB2; ARR2; BARR2

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat

Applications

Peptide ELISA, Western Blot

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27676)

Full Name

VPS35 Antibody, Clone 5A9: ATTO 594

Gene Names

VPS35; MEM3; PARK17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein G Purified

Pricing

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WB (Western Blot) (All lanes : Anti-DHCR7 Antibody (C-term) at 1:2000 dilution Lane 1: Caco2 whole cell lysateLane 2: mouse testis lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (please inquire) at 1/15000 dilution.Observed band size : 55kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

DHCR7, Polyclonal Antibody (Cat# AAA28753)

Full Name

DHCR7 Antibody (C-term)

Gene Names

DHCR7; SLOS

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of K562 cells using anti-CASP3 antibody (AAA11633).Overlay histogram showing K562 cells stained with AAA11633 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CASP3 Antibody (AAA11633,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Caspase-3, Polyclonal Antibody (Cat# AAA11633)

Full Name

Anti-Caspase-3 Antibody

Gene Names

CASP3; CPP32; SCA-1; CPP32B

Reactivity

Human, Mouse, Rat. No cross reactivity with other proteins.

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (SCNN1A Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

SCNN1A, Polyclonal Antibody (Cat# AAA28784)

Full Name

SCNN1A Antibody (Center)

Gene Names

SCNN1A; BESC2; ENaCa; SCNEA; SCNN1; ENaCalpha

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

ompT, Polyclonal Antibody (Cat# AAA26977)

Full Name

ompT Antibody

Reactivity

Escherichia coli

Applications

Western Blot

Purity

>95%, Protein G purified

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry: Human tonsil tissue (FFPE) was stained with Anti-CD20 antibody, (Cat# AAA14082) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)

CD20, Polyclonal Antibody (Cat# AAA14082)

Full Name

Rabbit anti CD20 antibody

Reactivity

Human

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry

Purity

The Rabbit IgG is purified by Epitope Affinity Purification.

Pricing

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APOE, Polyclonal Antibody (Cat# AAA23552)

Full Name

APOE antibody - N-terminal region

Gene Names

APOE; AD2; LPG; APO-E; ApoE4; LDLCQ5

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purified

Pricing

Cd90, Monoclonal Antibody (Cat# AAA13815)

Full Name

Cd90

Gene Names

THY1; CD90

Reactivity

Human, Monkey. Others not known.

Applications

Flow Cytometry, Immunofluorescence, Functional Assay

Pricing

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Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Annexin A1, Monoclonal Antibody (Cat# AAA23918)

Full Name

Annexin A1/(Hairy Cell Leukemia Marker)

Gene Names

ANXA1; ANX1; LPC1

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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CD14, Monoclonal Antibody (Cat# AAA12224)

Full Name

MOUSE ANTI HUMAN CD14:Biotin

Applications

Flow Cytometry

Pricing

Bordetella pertussis Pertactin autotransporter, Polyclonal Antibody (Cat# AAA18844)

Full Name

Rabbit anti-Bordetella pertussis Pertactin autotransporter polyclonal Antibody

Reactivity

Bordetella pertussis

Applications

EIA

Purity

>95%, Protein G purified

Pricing

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PLLP, Polyclonal Antibody (Cat# AAA26925)

Full Name

PLLP Antibody

Gene Names

PLLP; PMLP; TM4SF11

Reactivity

Human, Mouse

Applications

Western Blot, Immunofluorescence

Purity

>95%, Protein G purified

Pricing

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Application Data (Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.)

SLC26A3, Polyclonal Antibody (Cat# AAA23387)

Full Name

SLC26A3 antibody - middle region

Gene Names

SLC26A3; CLD; DRA

Reactivity

Tested Reactivity: Human
Predicted Reactivity: Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Western blot analysis of extracts from K562 cells(H2O2 treatment), using Phospho-EGFR (Tyr992)[Tyr1016] Ab. The lane on the left was treated with blocking peptide.)

EGFR, Polyclonal Antibody (Cat# AAA30961)

Full Name

Phospho-EGFR (Tyr1016) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

COMPLEMENT FACTOR I, Monoclonal Antibody (Cat# AAA11964)

Full Name

MOUSE ANTI HUMAN COMPLEMENT FACTOR I

Gene Names

CFI; FI; IF; KAF; AHUS3; ARMD13; C3BINA; C3b-INA

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry, Western Blot, Functional Assay

Pricing

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CD14, Monoclonal Antibody (Cat# AAA12087)

Full Name

MOUSE ANTI HUMAN CD14:RPE

Applications

Flow Cytometry

Pricing

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Structural Maintenance Of Chromosomes Protein 3 (SMC3), Polyclonal Antibody (Cat# AAA20309)

Full Name

Polyclonal Antibody to Structural Maintenance Of Chromosomes Protein 3 (SMC3)

Gene Names

SMC3; BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1

Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U87 cells using anti-JUNB antibody (AAA19249)Overlay histogram showing U87 cells stained with AAA19249 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JUNB Antibody (AAA19249, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

JUNB, Polyclonal Antibody (Cat# AAA19249)

Full Name

Anti-JUNB Antibody

Gene Names

JUNB; AP-1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

MYT1, ELISA Kit (Cat# AAA30732)

Full Name

MYT1 Colorimetric Cell-Based ELISA Kit

Gene Names

PKMYT1; MYT1; PPP1R126

Reactivity

Human

Pricing

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CD14, Monoclonal Antibody (Cat# AAA12086)

Full Name

MOUSE ANTI HUMAN CD14:FITC

Applications

Flow Cytometry

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of C6 cells using anti-KCNQ2 antibody. Overlay histogram showing C6 cells stained with (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNQ2 Antibody (1 ug/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

KCNQ2, Polyclonal Antibody (Cat# AAA19238)

Full Name

Anti-KCNQ2 Antibody

Gene Names

KCNQ2; EBN; BFNC; EBN1; ENB1; BFNS1; EIEE7; HNSPC; KV7.2; KCNA11; KVEBN1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of METTL3 using anti-METTL3 antibody (AAA19248).METTL3 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL3 Antibody (AAA19248) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)