Search results for "cancer"

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using ID3 antibody.)

ID3, Polyclonal Antibody (Cat# AAA28175)

Full Name

ID3 Polyclonal Antibody

Gene Names

ID3; HEIR-1; bHLHb25

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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DNAJB1, Polyclonal Antibody (Cat# AAA29711)

Full Name

DNAJB1 Antibody

Gene Names

DNAJB1; Hdj1; Sis1; HSPF1; Hsp40; RSPH16B

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

Steroid 21-hydroxylase (CYP21A2), Recombinant Protein (Cat# AAA18457)

Full Name

Recombinant Human Steroid 21-hydroxylase (CYP21A2)

Gene Names

CYP21A2; CAH1; CPS1; CA21H; CYP21; CYP21B; P450c21B

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using GAPDH Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

GAPDH, Polyclonal Antibody (Cat# AAA28397)

Full Name

GAPDH Rabbit pAb

Gene Names

GAPDH; G3PD; GAPD

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of Raji cells using anti-CD38 antibody (AAA19217).Overlay histogram showing Raji cells stained with AAA19217 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD38 Antibody (AAA19217, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

CD38, Polyclonal Antibody (Cat# AAA19217)

Full Name

Anti-CD38 Antibody

Gene Names

CD38; T10; ADPRC 1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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SUZ12, Polyclonal Antibody (Cat# AAA29851)

Full Name

SUZ12 Polyclonal Antibody

Gene Names

SUZ12; CHET9; JJAZ1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity purification

Pricing

SGK269, Antibody (Cat# AAA17976)

Full Name

SGK269 Antibody

Gene Names

PEAK1; SGK269

Reactivity

Human

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with Fragilis antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Fragilis, Monoclonal Antibody (Cat# AAA30394)

Full Name

Fragilis Antibody

Gene Names

IFITM3; 1-8U; IP15; DSPA2b

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using UHRF1 antibody. Blue: DAPI for nuclear staining.)

UHRF1, Polyclonal Antibody (Cat# AAA10713)

Full Name

UHRF1 Polyclonal Antibody

Gene Names

UHRF1; Np95; hNP95; ICBP90; RNF106; hUHRF1; huNp95

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Western blot analysis of MRPL12 over-expressed 293 cell line, cotransfected with MRPL12 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MRPL12 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

MRPL12, Monoclonal Antibody (Cat# AAA24575)

Full Name

MRPL12 (Mitochondrial Ribosomal Protein L12, 39S Ribosomal Protein L12, Mitochondrial, 5c5-2, FLJ60124, L12mt, MGC8610, MRP-L12, MRP-L31/34, MRPL7, MRPL7/L12, RPML12) APC

Gene Names

MRPL12; 5c5-2; L12mt; MRPL7; RPML12; MRPL7/L12; MRP-L31/34

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of K562 cells using anti-IRF3 antibody (AAA19216).Overlay histogram showing K562 cells stained with AAA19216 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IRF3 Antibody (AAA19216, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IRF3, Polyclonal Antibody (Cat# AAA19216)

Full Name

Anti-IRF3 Antibody

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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LSM8, Polyclonal Antibody (Cat# AAA19337)

Full Name

Anti-LSM8 Antibody

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of SiHa cells using anti-Aconitase 1/ACO1 antibody (AAA19267).Overlay histogram showing SiHa cells stained with AAA19267 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aconitase 1/ACO1 Antibody (AAA19267,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Aconitase 1/ACO1, Polyclonal Antibody (Cat# AAA19267)

Full Name

Anti-Aconitase 1/ACO1 Antibody

Gene Names

ACO1; IRP1; ACONS; IREB1; IREBP; IREBP1

Reactivity

Human, Mouse, Monkey, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

Transforming Growth Factor Alpha, ELISA Kit (Cat# AAA16894)

Full Name

Bovine Transforming Growth Factor Alpha ELISA Kit

Gene Names

TGFA; TFGA

Reactivity

Bovine

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat bronchus cells using KRT7 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

KRT7, Polyclonal Antibody (Cat# AAA22219)

Full Name

KRT7 Polyclonal Antibody

Gene Names

KRT7; K7; CK7; SCL; K2C7

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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eIF2alpha, Antibody (Cat# AAA17981)

Full Name

eIF2alpha (Ab-51) Antibody

Gene Names

EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cell using MAPK1 antibody. Blue: DAPI for nuclear staining.)

MAPK1, Polyclonal Antibody (Cat# AAA29678)

Full Name

MAPK1 Antibody

Gene Names

MAPK1; ERK; p38; p40; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug HIST3H3 antibody. Western blot was performed from the immunoprecipitate using HIST3H3 antibody at a dilition of 1:500.)

HIST3H3, Polyclonal Antibody (Cat# AAA28284)

Full Name

HIST3H3 Polyclonal Antibody

Gene Names

HIST3H3; H3t; H3.4; H3/g; H3FT

Reactivity

Human, Mouse, Rat, Other (Wide Range)

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with CK5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)

CK5, Monoclonal Antibody (Cat# AAA29898)

Full Name

CK5 Antibody

Gene Names

KRT5; K5; CK5; DDD; EBS2; KRT5A

Reactivity

Human, Mouse, Monkey

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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WB (Western Blot) (Western blot of anti-CD44 mAb against HeLa (1) and HUVE-12(2) cell lysate.)

CD44, Monoclonal Antibody (Cat# AAA12358)

Full Name

Mouse Monoclonal [clone 8E2F3] (IgG1) to Human CD44

Gene Names

CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry

Purity

Ascites

Pricing

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IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)

OPN-a/b, Polyclonal Antibody (Cat# AAA26857)

Full Name

OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (APC)

Gene Names

SPP1; OPN; BNSP; BSPI; ETA-1

Reactivity

Human

Applications

WB, IHC, IF

Purity

Purified by Protein A and Peptide Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of PC-3 cells using anti-MitoNEET/CISD1 antibody (AAA19293).Overlay histogram showing PC-3 cells stained with AAA19293 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MitoNEET/CISD1 Antibody (AAA19293,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MitoNEET/CISD1, Polyclonal Antibody (Cat# AAA19293)

Full Name

Anti-MitoNEET/CISD1 Antibody

Gene Names

CISD1; ZCD1; MDS029; C10orf70; mitoNEET

Reactivity

Human, Mouse, Rat, Monkey

Applications

WB, IHC-P, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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ICC (Immunocytochemistry) (ICC staining VAMP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

VAMP1, Monoclonal Antibody (Cat# AAA30520)

Full Name

VAMP1 Antibody

Gene Names

VAMP1; SYB1; SPAX1; VAMP-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

ProA affinity purified

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with Huntingtin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Huntingtin, Monoclonal Antibody (Cat# AAA30460)

Full Name

Huntingtin Antibody

Gene Names

HTT; HD; IT15

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS, ICC

Purity

ProA affinity purified

Pricing

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ICC (Immunocytochemistry) (ICC staining RANTES in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

RANTES, Monoclonal Antibody (Cat# AAA30380)

Full Name

RANTES Antibody

Gene Names

CCL5; SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Purity

ProA affinity purified

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)

Hsp70/Hsc70, Monoclonal Antibody (Cat# AAA17791)

Full Name

Hsp70/Hsc70 Antibody: PerCP

Gene Names

HSPA2; HSP70

Reactivity

Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast

Applications

WB, IP, IHC

Pricing

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Liver Cancer Tissue.)

Glycoprotein 39, Cartilage (GP39), Polyclonal Antibody (Cat# AAA20103)

Full Name

Polyclonal Antibody to Glycoprotein 39, Cartilage (GP39)

Gene Names

CHI3L1; GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39

Reactivity

Human

Applications

ICC, IHC, EIA, WB

Purity

Affinity Chromatography

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using ZNF433 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

ZNF433, Polyclonal Antibody (Cat# AAA22186)

Full Name

ZNF433 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

IHC, IF

Purity

Affinity purification

Pricing

Beclin 1, ELISA Kit (Cat# AAA16637)

Full Name

Human Beclin 1 ELISA Kit

Gene Names

BECN1; ATG6; VPS30; beclin1

Reactivity

Human

Pricing

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Application Data (Detection limit for recombinant GST tagged NODAL is ~0.3ng/ml as a capture antibody.)

NODAL, Monoclonal Antibody (Cat# AAA25180)

Full Name

NODAL (Nodal Homolog, MGC138230) (FITC)

Gene Names

NODAL; HTX5

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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SMARCE1, Polyclonal Antibody (Cat# AAA28107)

Full Name

SMARCE1 Polyclonal Antibody

Gene Names

SMARCE1; BAF57

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation

Purity

Affinity Purification

Pricing

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IHC (Immunohistchemistry) (DAB staining on IHC-P; Samples: Human Pancreas Tissue.)

Butyrylcholinesterase (BCHE), Polyclonal Antibody (Cat# AAA20094)

Full Name

Polyclonal Antibody to Butyrylcholinesterase (BCHE)

Gene Names

BCHE; E1; CHE1; CHE2

Reactivity

Human, Rat

Applications

ICC, IHC, EIA, WB

Purity

Affinity Chromatography

Pricing

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MTUS1, Polyclonal Antibody (Cat# AAA23518)

Full Name

MTUS1 antibody - middle region

Gene Names

MTUS1; ATBP; ATIP; ICIS; MP44; ATIP3; MTSG1

Reactivity

Cow, Dog, Guinea Pig, Human, Mouse, Rabbit, Rat

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of HP1 gamma using anti-HP1 gamma antibody (AAA19145).HP1 gamma was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HP1 gamma Antibody (AAA19145) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

HP1 gamma, Polyclonal Antibody (Cat# AAA19145)

Full Name

Anti-HP1 gamma Picoband antibody

Gene Names

CBX3; HECH; HP1-GAMMA; HP1Hs-gamma

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

EIA, IHC, WB

Pricing

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IF (Immunofluorescence) (Figure 6. IF analysis of GRSF1 using anti- GRSF1 antibody (AAA19315).GRSF1 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- GRSF1 Antibody (AAA19315) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

GRSF1, Polyclonal Antibody (Cat# AAA19315)

Full Name

Anti-GRSF1 Antibody

Reactivity

Human

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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TIMM8A/DDP, Polyclonal Antibody (Cat# AAA19317)

Full Name

Anti-TIMM8A/DDP Antibody

Gene Names

TIMM8A; DDP; MTS; DDP1; DFN1; TIM8

Reactivity

Human, Mouse, Rat, Monkey

Applications

WB, IHC-P, ICC, IF, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of K562 cells using anti-HP1 alpha/CBX5 antibody (AAA19266).Overlay histogram showing K562 cells stained with AAA19266 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HP1 alpha/CBX5 Antibody (AAA19266,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

HP1 alpha/CBX5, Polyclonal Antibody (Cat# AAA19266)

Full Name

Anti-HP1 alpha/CBX5 Antibody

Gene Names

CBX5; HP1; HP1A

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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VPRBP/DCAF1, Polyclonal Antibody (Cat# AAA19343)

Full Name

Anti-VPRBP/DCAF1 Antibody

Gene Names

DCAF1; RIP; VPRBP

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

Tumor Necrosis Factor alpha, ELISA Kit (Cat# AAA16584)

Full Name

Guinea pig Tumor Necrosis Factor alpha ELISA Kit

Gene Names

TNF; DIF; TNFA; TNFSF2; TNF-alpha

Reactivity

Guinea Pig

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)

MAGEA12, Monoclonal Antibody (Cat# AAA24558)

Full Name

MAGEA12 (Melanoma-associated Antigen 12, MAGE-12 Antigen, MAGE-12F Antigen, Cancer/Testis Antigen 1.12, CT1.12, MAGE12) APC

Gene Names

MAGEA12; CT1.12; MAGE12

Reactivity

Human, Mouse

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

Yorkie homolog (YAP1), Recombinant Protein (Cat# AAA15932)

Full Name

Recombinant Human Yorkie homolog (YAP1)

Gene Names

YAP1; YAP; YKI; COB1; YAP2; YAP65

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.)

Calcyclin, Polyclonal Antibody (Cat# AAA20888)

Full Name

Polyclonal Antibody to Calcyclin Binding Protein (CACYBP)

Gene Names

CACYBP; SIP; GIG5; PNAS-107; S100A6BP

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, ICC, IHC, EIA

Purity

Affinity Chromatography

Pricing

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IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)

OPN-a/b, Polyclonal Antibody (Cat# AAA26861)

Full Name

OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (MaxLight 490)

Gene Names

SPP1; OPN; BNSP; BSPI; ETA-1

Reactivity

Human

Applications

WB, IHC, IF

Purity

Purified by Protein A and Peptide Affinity Chromatography.

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of Cytoglobin using anti- Cytoglobin antibody (AAA11681).Cytoglobin was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Cytoglobin Antibody (AAA11681) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Cytoglobin, Polyclonal Antibody (Cat# AAA11681)

Full Name

Anti-Cytoglobin Antibody

Gene Names

CYGB; HGB; STAP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HL-60 cells using anti- MCM2 antibody (AAA19351).Overlay histogram showing HL-60 cells stained with AAA19351 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-MCM2 Antibody (AAA19351, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)