Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
93 results for "Heat Shock Proteins" - showing 50-93
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
PDIA6, Recombinant Protein (Cat# AAA11803)
Full Name
PDIA6, 20-440aa, Human, His tag, E Coli
Gene Names
PDIA6; P5; ERP5; TXNDC7
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
IP (Immunoprecipitation)
(Hspb7 was immunoprecipitated using: Lane A:0.5 mg NIH-3T3 Whole Cell Lysate Lane B:0.5 mg A549 Whole Cell Lysate 4 uL anti-Hspb7 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Hspb7 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 19 kDa Observed band size: 22 kDa)
HSPB7, Polyclonal Antibody (Cat# AAA27732)
Full Name
Anti-HSPB7 Antibody, Rabbit Polyclonal
Gene Names
Hspb7; 27kDa; cvHsp; Hsp25-2
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Western Blot detection against Immunogen (85.84kD).)
STIP1, Monoclonal Antibody (Cat# AAA24969)
Full Name
STIP1 (Stress-induced-phosphoprotein 1, STI1, Hsc70/Hsp90-organizing Protein, Hop, P60, Renal Carcinoma Antigen NY-REN-11, STI1L, Transformation-sensitive Protein IEF SSP 3521, IEF-SSP-3521) (Biotin)
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Reactivity
Human
Applications
EIA, IF, IHC, IP, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Detection limit for recombinant GST tagged DNAJB6 is ~1ng/ml as a capture antibody.)
DNAJB6, Monoclonal Antibody (Cat# AAA25080)
Full Name
DNAJB6 (HSJ2, MRJ, MSJ1, DnaJ Homolog Subfamily B Member 6, HHDJ1, Heat Shock Protein J2, HSJ-2, MSJ-1, MGC1152, MGC117297, DKFZp566D0824, FLJ42837) (FITC)
Gene Names
DNAJB6; DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E; LGMDD1
Reactivity
Human
Applications
EIA, IF, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western Blot detection against Immunogen (85.84kD).)
STIP1, Monoclonal Antibody (Cat# AAA25559)
Full Name
STIP1 (Stress-induced-phosphoprotein 1, STI1, Hsc70/Hsp90-organizing Protein, Hop, P60, Renal Carcinoma Antigen NY-REN-11, STI1L, Transformation-sensitive Protein IEF SSP 3521, IEF-SSP-3521) (HRP)
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Reactivity
Human
Applications
EIA, IHC, IP, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
FCM (Flow Cytometry)
(Flow cytometry analysis of Hsp105alpha in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
HSP105alpha, Monoclonal Antibody (Cat# AAA11727)
Full Name
HSP105alpha antibody
Gene Names
HSPH1; HSP105; HSP105A; HSP105B; NY-CO-25
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry
Purity
By protein-G affinity chromatography
Pricing
WB (Western Blot)
(HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in PC-12.)
HSPA1L, Monoclonal Antibody (Cat# AAA24242)
Full Name
HSPA1L (Heat Shock 70kD Protein 1-like, HSP70-Hom, Heat shock 70kD protein 1L, Heat Shock 70kD Protein 1-Hom) (AP)
Gene Names
HSPA1L; HSP70T; hum70t; HSP70-1L; HSP70-HOM
Reactivity
Human, Rat
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western Blot detection against Immunogen (37.84kD).)
HSPB1, Monoclonal Antibody (Cat# AAA24243)
Full Name
HSPB1 (Heat Shock Protein beta-1, HspB1, Heat Shock 27kD Protein, HSP 27, Stress-responsive Protein 27, SRP27, Estrogen-regulated 24kD Protein, 28kD Heat Shock Protein, HSPB1, HSP27) (AP)
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human
Applications
EIA, IP, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Detection limit for recombinant GST tagged DNAJB6 is ~1ng/ml as a capture antibody.)
DNAJB6, Monoclonal Antibody (Cat# AAA24488)
Full Name
DNAJB6 (HSJ2, MRJ, MSJ1, DnaJ Homolog Subfamily B Member 6, HHDJ1, Heat Shock Protein J2, HSJ-2, MSJ-1, MGC1152, MGC117297, DKFZp566D0824, FLJ42837) APC
Gene Names
DNAJB6; DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E; LGMDD1
Reactivity
Human
Applications
EIA, IF, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
FCM (Flow Cytometry)
(Figure 13. Flow Cytometry analysis of THP-1 cells using anti-Alpha B Crystallin/CRYAB antibody (AAA19277).Overlay histogram showing THP-1 cells stained with AAA19277 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha B Crystallin/CRYAB Antibody (AAA19277, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Alpha B Crystallin/CRYAB, Polyclonal Antibody (Cat# AAA19277)
Full Name
Anti-Alpha B Crystallin/CRYAB Antibody
Gene Names
CRYAB; CRYA2; CTPP2; HSPB5; CMD1II
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western Blot detection against Immunogen (37.84kD).)
HSPB1, Monoclonal Antibody (Cat# AAA24539)
Full Name
HSPB1 (Heat Shock Protein beta-1, HspB1, Heat Shock 27kD Protein, HSP 27, Stress-responsive Protein 27, SRP27, Estrogen-regulated 24kD Protein, 28kD Heat Shock Protein, HSPB1, HSP27) APC
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human
Applications
EIA, IF, IP, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in HeLa.)
DNAJC10, Monoclonal Antibody (Cat# AAA24784)
Full Name
DNAJC10 (DnaJ Homolog Subfamily C Member 10, ER-resident Protein ERdj5, Macrothioredoxin, MTHr, ERDJ5, UNQ495/PRO1012, DKFZp434J1813, MGC104194) (Biotin)
Gene Names
DNAJC10; JPDI; MTHr; ERdj5; PDIA19
Reactivity
Human, Mouse, Rat
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in PC-12.)
HSPA1L, Monoclonal Antibody (Cat# AAA24538)
Full Name
HSPA1L (Heat Shock 70kD Protein 1-like, HSP70-Hom, Heat shock 70kD protein 1L, Heat Shock 70kD Protein 1-Hom) APC
Gene Names
HSPA1L; HSP70T; hum70t; HSP70-1L; HSP70-HOM
Reactivity
Human, Rat
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western Blot detection against Immunogen (85.84kD).)
STIP1, Monoclonal Antibody (Cat# AAA25265)
Full Name
STIP1 (Stress-induced-phosphoprotein 1, STI1, Hsc70/Hsp90-organizing Protein, Hop, P60, Renal Carcinoma Antigen NY-REN-11, STI1L, Transformation-sensitive Protein IEF SSP 3521, IEF-SSP-3521) (FITC)
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Reactivity
Human
Applications
EIA, IF, IHC, IP, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using Bip/GRP78 (6H7) Mouse mAb.)
GRP78 BiP, Monoclonal Antibody (Cat# AAA23991)
Full Name
GRP78 BiP (6H7) Mouse mAb
Gene Names
HSPA5; BIP; MIF2; GRP78
Reactivity
Human, Rat
Applications
WB, IHC
Purity
Affinity purified
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HELA cells using anti-DNAJC10 antibody (AAA19320).Overlay histogram showing HELA cells stained with AAA19320 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNAJC10 Antibody (AAA19320, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DNAJC10, Polyclonal Antibody (Cat# AAA19320)
Full Name
Anti-DNAJC10 Antibody
Gene Names
DNAJC10; JPDI; MTHr; ERdj5; PDIA19
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in HeLa.)
DNAJC10, Monoclonal Antibody (Cat# AAA14783)
Full Name
DNAJC10 (DnaJ Homolog Subfamily C Member 10, ER-resident Protein ERdj5, Macrothioredoxin, MTHr, ERDJ5, UNQ495/PRO1012, DKFZp434J1813, MGC104194)
Gene Names
DNAJC10; JPDI; MTHr; ERdj5; PDIA19
Reactivity
Human, Mouse, Rat
Applications
EL/EIA, WB, IHC, IF
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
Application Data
(Detection limit for recombinant GST tagged DNAJB6 is ~1ng/ml as a capture antibody.)
DNAJB6, Monoclonal Antibody (Cat# AAA24783)
Full Name
DNAJB6 (HSJ2, MRJ, MSJ1, DnaJ Homolog Subfamily B Member 6, HHDJ1, Heat Shock Protein J2, HSJ-2, MSJ-1, MGC1152, MGC117297, DKFZp566D0824, FLJ42837) (Biotin)
Gene Names
DNAJB6; DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E; LGMDD1
Reactivity
Human
Applications
EIA, IF, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in HeLa.)
DNAJC10, Monoclonal Antibody (Cat# AAA24489)
Full Name
DNAJC10 (DnaJ Homolog Subfamily C Member 10, ER-resident Protein ERdj5, Macrothioredoxin, MTHr, ERDJ5, UNQ495/PRO1012, DKFZp434J1813, MGC104194) APC
Gene Names
DNAJC10; JPDI; MTHr; ERdj5; PDIA19
Reactivity
Human, Mouse, Rat
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in HeLa.)
DNAJC10, Monoclonal Antibody (Cat# AAA25081)
Full Name
DNAJC10 (DnaJ Homolog Subfamily C Member 10, ER-resident Protein ERdj5, Macrothioredoxin, MTHr, ERDJ5, UNQ495/PRO1012, DKFZp434J1813, MGC104194) (FITC)
Gene Names
DNAJC10; JPDI; MTHr; ERdj5; PDIA19
Reactivity
Human, Mouse, Rat
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western Blot detection against Immunogen (85.84kD).)
STIP1, Monoclonal Antibody (Cat# AAA25855)
Full Name
STIP1 (Stress-induced-phosphoprotein 1, STI1, Hsc70/Hsp90-organizing Protein, Hop, P60, Renal Carcinoma Antigen NY-REN-11, STI1L, Transformation-sensitive Protein IEF SSP 3521, IEF-SSP-3521) (PE)
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Reactivity
Human
Applications
EIA, IF, IHC, IP, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of A549 cells using anti-Hsp90 alpha antibody (AAA19359).Overlay histogram showing A549 cells stained with AAA19359 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Hsp90 alpha Antibody (AAA19359, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Hsp90 alpha, Monoclonal Antibody (Cat# AAA19359)
Full Name
Anti-Hsp90 alpha Antibody (monoclonal, 6B5)
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Paraffin embedded sections of human urothelium were incubated with anti-human Hsp90 (1:100)for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detectedusing Diaminobenzidine (DAB))
Hsp90, Monoclonal Antibody (Cat# AAA11726)
Full Name
Hsp90 antibody
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
Reactivity
Human,
Applications
Western Blot, Immunohistochemistry
Purity
By protein-G affinity chromatography
Pricing
FCM (Flow Cytometry)
(Flow cytometry analysis of Crystallin alpha A in Balb/3T3 cells. The cell was stained with AAA11723 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
A crystallin A, Monoclonal Antibody (Cat# AAA11723)
Full Name
A crystallin A antibody
Gene Names
CRYAA; CRYA1; HSPB4; CTRCT9
Reactivity
Human
Applications
Western Blot, Flow Cytometry
Purity
By protein-G affinity chromatography
Pricing
SDS-PAGE
HSP70, Recombinant Protein (Cat# AAA11739)
Full Name
HSP70, 1-641aa, Human, His tag, E Coli
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
Heat Shock 70kDa Protein-8, Recombinant Protein (Cat# AAA10869)
Full Name
Recombinant Human Heat Shock 70kDa Protein-8
Gene Names
HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
Purity
Greater than 90.0% as determined by SDS-PAGE.
Pricing
WB (Western Blot)
(HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in PC-12.)
HSPA1L, Monoclonal Antibody (Cat# AAA24833)
Full Name
HSPA1L (Heat Shock 70kD Protein 1-like, HSP70-Hom, Heat shock 70kD protein 1L, Heat Shock 70kD Protein 1-Hom) (Biotin)
Gene Names
HSPA1L; HSP70T; hum70t; HSP70-1L; HSP70-HOM
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western Blot Analysis of human MCF-7 cell lysate using ER-beta1 Mouse Monoclonal Antibody (ERb455).)
ER-beta1 (Estrogen Receptor beta-1), Monoclonal Antibody (Cat# AAA13814)
Full Name
ER-beta1 (Estrogen Receptor beta-1) Mouse Monoclonal Antibody
Gene Names
ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12081)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
Western Blot
Pricing
SDS-PAGE
(3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.)
GroEL, Recombinant Protein (Cat# AAA11737)
Full Name
GroEL, 1-548aa, E Coli, E Coli
Gene Names
groL; ECK4137; groEL; JW4103; mopA
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11864)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
Immunofluorescence
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11930)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
SDS-PAGE
(3 ug by SDS-PAGE under reducing condition and visualized by coomasie blue stain)
HSP10, Recombinant Protein (Cat# AAA11744)
Full Name
HSP10, 1-102aa, Human, E Coli
Gene Names
HSPE1; EPF; CPN10; GROES; HSP10
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12211)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
WB (Western Blot)
(HSPA1B monoclonal antibody (M05), clone 2D11. Western Blot analysis of HSPA1B expression in PC-12.)
HSPA1B, Monoclonal Antibody (Cat# AAA26112)
Full Name
HSPA1B (Heat Shock 70kD Protein 1B, FLJ54328, HSP70-1B, HSP70-2, HSPA1A) (APC)
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
Applications
Immunohistochemistry, Western Blot
Purity
Purified
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11850)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
Immunohistochemistry, Western Blot
Pricing
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
HSP90B1 (GRP94), Recombinant Protein (Cat# AAA11758)
Full Name
HSP90B1 (GRP94), 22-803aa, Human, His tag, E Coli
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
SDS-PAGE
Heme oxygenase1, Recombinant Protein (Cat# AAA11763)
Full Name
Heme oxygenase1,1-266aa, Human, His tag, E Coli
Gene Names
HMOX1; HO-1; HSP32; HMOX1D; bK286B10
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
HSP47, Recombinant Protein (Cat# AAA11757)
Full Name
HSP47 (Colligin), 18-418aa, Human, His tag, E Coli
Gene Names
SERPINH1; CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
PDIA3, Recombinant Protein (Cat# AAA11788)
Full Name
PDIA3, 25-505aa, Human, His tag, E Coli
Gene Names
PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
WB (Western Blot)
(HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in Raw 264.7.)
HSPA1A, Monoclonal Antibody (Cat# AAA24832)
Full Name
HSPA1A (Heat Shock 70kD Protein 1A/1B, Heat Shock 70kD Protein 1/2, HSP70.1/HSP70.2, HSP70-1/HSP70-2, HSPA1, HSPA1B) (Biotin)
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
DNAJC10, Monoclonal Antibody (Cat# AAA24193)
Full Name
DNAJC10 (DnaJ Homolog Subfamily C Member 10, ER-resident Protein ERdj5, Macrothioredoxin, MTHr, ERDJ5, UNQ495/PRO1012, DKFZp434J1813, MGC104194) (AP)
Gene Names
DNAJC10; JPDI; MTHr; ERdj5; PDIA19
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
DNAJB6, Monoclonal Antibody (Cat# AAA24192)
Full Name
DNAJB6 (HSJ2, MRJ, MSJ1, DnaJ Homolog Subfamily B Member 6, HHDJ1, Heat Shock Protein J2, HSJ-2, MSJ-1, MGC1152, MGC117297, DKFZp566D0824, FLJ42837) (AP)
Gene Names
DNAJB6; DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E; LGMDD1
Reactivity
Human
Applications
EIA, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing