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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
61 results for "Culture Media" - showing 50-61
Staining Procedure
(1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides.2. Air dry for 2 hours at 58° C.3. Deparaffinize, dehydrate and rehydrate tissues.4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.5. Any of three heating methods may be used:a. TintoRetriever Pressure Cooker or EquivalentPlace tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. TintoRetriever PT Module or Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.c. Conventional Steamer MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. 8. Wash slides with ImmunoDNA washer or DI water. 9. Continue IHC staining protocol.)
Podoplanin/D2-40, Monoclonal Antibody (Cat# AAA13558)
Full Name
Podoplanin/D2-40
Reactivity
Human, Rat, Mouse
Applications
Immunohistochemistry
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12093)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA
Reactivity
Dog, Dolphin, Ferret, Fin Whale, Goat, Horse, Human, Mink, Rabbit, Pig, Sheep.
Based on sequence similarity, is expected to react with: Mustelid
N.B. Antibody reactivity and working conditions may vary between species.
Based on sequence similarity, is expected to react with: Mustelid
N.B. Antibody reactivity and working conditions may vary between species.
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12096)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:RPE
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12095)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:FITC
Applications
Flow Cytometry
Pricing
Quality Control
(This antibody has been quality control tested by Immunohistochemistry as follows.)
Application Data
TCR Beta F1, Monoclonal Antibody (Cat# AAA13568)
Full Name
TCR Beta F1
Reactivity
Human
Applications
Immunohistochemistry
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12094)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:Biotin
Applications
Flow Cytometry
Pricing
6xHIS, Monoclonal Antibody (Cat# AAA14456)
Full Name
Purified Mouse anti-6xHIS (CLONE 7B8)-Monoclonal
Applications
Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Purified Mouse anti- 6xHIS Tag .
Application Data
IgE, Monoclonal Antibody (Cat# AAA14454)
Full Name
Purified Mouse anti-Dog IgE (Clone E671)
Reactivity
Dog
Applications
Western Blot
Standard Curve (Sample)
Glucoamylase, Assay Kit (Cat# AAA27904)
Full Name
Glucoamylase Microplate Assay Kit
Reactivity
General
Applications
Functional Assay
Pricing
IHC (Immunohistochemistry)
(IHC of Parvovirus B19 on a FFPE Infected Placenta Tissue)
Parvovirus, Monoclonal Antibody (Cat# AAA13563)
Full Name
Parvovirus
Reactivity
Human
Applications
Immunohistochemistry
Pricing