Search results for "Cell Tissues Lysates"

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of PC-3 cells using anti-ADK antibody (AAA11690).Overlay histogram showing PC-3 cells stained with AAA11690 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADK Antibody (AAA11690,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ADK, Polyclonal Antibody (Cat# AAA11690)

Full Name

Anti-ADK Antibody

Gene Names

ADK; AK

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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MYD88, Polyclonal Antibody (Cat# AAA10930)

Full Name

MYD88 Antibody

Gene Names

MYD88; MYD88D

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

MYD88 Antibody is affinity chromatography purified via peptide column.

Pricing

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

p27 Kip1, Polyclonal Antibody (Cat# AAA31388)

Full Name

Phospho-p27 Kip1 (Ser178) Antibody

Gene Names

CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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hRIP3, Polyclonal Antibody (Cat# AAA11031)

Full Name

hRIP3 Antibody

Gene Names

RIPK3; RIP3

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

hRIP3 Antibody is Protein A purified.

Pricing

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

TLK1, Polyclonal Antibody (Cat# AAA31385)

Full Name

Phospho-TLK1 (Ser741) Antibody

Gene Names

TLK1; PKU-beta

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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RAD17, Polyclonal Antibody (Cat# AAA31431)

Full Name

Phospho-RAD17 (Ser646) Antibody

Gene Names

RAD17; CCYC; R24L; RAD24; HRAD17; RAD17SP

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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CD227/MUC1, Polyclonal Antibody (Cat# AAA31426)

Full Name

Phospho-CD227/MUC1 (Ser1227) Antibody

Gene Names

MUC1; EMA; PEM; PUM; KL-6; MAM6; PEMT; CD227; H23AG; MUC-1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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PDX1, Polyclonal Antibody (Cat# AAA31430)

Full Name

Phospho-PDX1 (Ser66) Antibody

Gene Names

PDX1; GSF; IPF1; IUF1; IDX-1; MODY4; PDX-1; STF-1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (88%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (88%), Xenopus (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (TECTA was detected in paraffin-embedded sections of human testis tissues using rabbit anti- TECTA Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

TECTA, Polyclonal Antibody (Cat# AAA11686)

Full Name

Anti-TECTA Antibody

Gene Names

TECTA; DFNA8; DFNA12; DFNB21

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TWF1 mouse mAb with DAB staining.)

Twinfilin-1, Monoclonal Antibody (Cat# AAA14123)

Full Name

Anti-Twinfilin-1 Mouse mAb

Gene Names

TWF1; A6; PTK9

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

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IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

CAMKK1/2, Polyclonal Antibody (Cat# AAA31402)

Full Name

Phospho-CAMKK1/2 (Ser458/Ser495) Antibody

Gene Names

CAMKK1; CAMKKA

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)

JAK3, Polyclonal Antibody (Cat# AAA31404)

Full Name

Phospho-JAK3 (Tyr904) Antibody

Gene Names

JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Sheep (100%), Dog (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using B2M mouse mAb with DAB staining.)

beta2-microglobulin, Monoclonal Antibody (Cat# AAA14146)

Full Name

Anti-beta2-microglobulin Mouse mAb

Gene Names

B2M; IMD43

Reactivity

Human

Applications

Western Blot

Pricing

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CAMK2G mouse mAb with DAB staining.)

CaMKII, Monoclonal Antibody (Cat# AAA14148)

Full Name

Anti-CaMKII Mouse mAb

Gene Names

CAMK2G; CAMK; CAMKG; CAMK-II

Reactivity

Human, Rat

Applications

Western Blot

Pricing

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NR6A1 mouse mAb with DAB staining.)

GCNF, Monoclonal Antibody (Cat# AAA14121)

Full Name

Anti-GCNF Mouse mAb

Gene Names

NR6A1; RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF

Reactivity

Human

Applications

Western Blot, Immunofluorescence

Pricing

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WB (Western Blot) (Western blot of Latexin (arrow) in 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LXN gene (Lane 2))

Latexin / MUM, Polyclonal Antibody (Cat# AAA12323)

Full Name

Rabbit Polyclonal to Human Latexin / MUM

Gene Names

LXN; ECI; TCI

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Flow Cytometry

Purity

Immunoaffinity Purified

Pricing

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WB (Western Blot) (Western blot analysis of LIN28B Antibody (N-term) in HL60 cell line lysates (35 ug/lane). LIN28B (arrow) was detected using the purified Pab.)

LIN28B, Polyclonal Antibody (Cat# AAA12320)

Full Name

Rabbit Polyclonal to Human LIN28B

Gene Names

LIN28B; CSDD2

Reactivity

Human

Applications

Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry

Purity

Ammonium sulfate precipitation

Pricing

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IHC (Immunohistchemistry) (At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

TOPBP1, Polyclonal Antibody (Cat# AAA31467)

Full Name

Phospho-TOPBP1 (Ser1159) Antibody

Gene Names

TOPBP1; TOP2BP1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (80%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (80%), Xenopus (80%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

p27 Kip1, Polyclonal Antibody (Cat# AAA31387)

Full Name

Phospho-p27 Kip1 (Thr157) Antibody

Gene Names

CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (89%), Sheep (89%), Rabbit (100%), Dog (100%), Chicken (89%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IF (Immunofluorescence) (Figure 7 Immunofluorescence Validation of TMPRSS2 in Rat BrainImmunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling TMPRSS2 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

TMPRSS2, Polyclonal Antibody (Cat# AAA11038)

Full Name

TMPRSS2 (IN) Antibody

Gene Names

TMPRSS2; PP9284; PRSS10

Reactivity

Human, Mouse, Rat
Predicted species reactivity based on immunogen sequence: Horse (100%); Rabbit (100%); Monkey (100%); Sheep (100%); Gorilla (100%); Cat (100%).

Applications

Immunofluorescence, Western Blot

Purity

TMPRSS2 Antibody is affinity chromatography purified via peptide column.

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of ADA using anti-ADA antibody (AAA19140).ADA was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ADA Antibody (AAA19140) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

ADA/Adenosine Deaminase, Polyclonal Antibody (Cat# AAA19140)

Full Name

Anti-ADA/Adenosine Deaminase Picoband antibody

Reactivity

Mouse, Rat
No cross reactivity with other proteins.

Applications

EIA, IHC, WB

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U20S cells using anti-NFIA antibody (AAA11691).Overlay histogram showing U20S cells stained with AAA11691 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFIA Antibody (AAA11691,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

NFIA, Polyclonal Antibody (Cat# AAA11691)

Full Name

Anti-NFIA Antibody

Gene Names

NFIA; CTF; NF1-A; NFI-A; NFI-L; NF-I/A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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TCP1 delta, Polyclonal Antibody (Cat# AAA11672)

Full Name

Anti-TCP1 delta Antibody

Gene Names

CCT4; SRB; Cctd; CCT-DELTA

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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JAK3, Polyclonal Antibody (Cat# AAA31405)

Full Name

Phospho-JAK3 (Tyr981) Antibody

Gene Names

JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN

Reactivity

Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (100%), Bovine (91%), Horse (100%), Sheep (100%), Dog (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of CRM1 using anti-CRM1 antibody (AAA11673).CRM1 was detected in frozen section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CRM1 Antibody (AAA11673) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

CRM1, Polyclonal Antibody (Cat# AAA11673)

Full Name

Anti-CRM1 Antibody

Gene Names

XPO1; emb; CRM1; exp1

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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CHRNA5, Polyclonal Antibody (Cat# AAA11685)

Full Name

Anti-CHRNA5 Antibody

Gene Names

CHRNA5; LNCR2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Periplakin was detected in paraffin-embedded sections of human oesophagus squama cancer tissues using rabbit anti- Periplakin Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

Periplakin, Polyclonal Antibody (Cat# AAA11684)

Full Name

Anti-Periplakin Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RANBP9 mouse mAb with DAB staining.)

RanBP9, Monoclonal Antibody (Cat# AAA14156)

Full Name

Anti-RanBP9 Mouse mAb

Gene Names

RANBP9; BPM-L; BPM90; RANBPM; RanBP7

Reactivity

Human, Rat

Applications

Western Blot

Pricing

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using UBE2I mouse mAb with DAB staining.)

Ubc9, Monoclonal Antibody (Cat# AAA14109)

Full Name

Anti-Ubc9 Mouse mAb

Gene Names

UBE2I; P18; UBC9; C358B7.1

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of Cytoglobin using anti- Cytoglobin antibody (AAA11681).Cytoglobin was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Cytoglobin Antibody (AAA11681) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Cytoglobin, Polyclonal Antibody (Cat# AAA11681)

Full Name

Anti-Cytoglobin Antibody

Gene Names

CYGB; HGB; STAP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of CPI17 alpha using anti- CPI17 alpha antibody (AAA19176).CPI17 alpha was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- CPI17 alpha Antibody (AAA19176) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

CPI17 alpha, Polyclonal Antibody (Cat# AAA19176)

Full Name

Anti-CPI17 alpha Picoband Antibody

Gene Names

PPP1R14A; CPI17; CPI-17; PPP1INL

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins

Applications

IHC, WB

Purity

Immunogen affinity purified

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (AAA19172).Musashi 1/Msi1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Musashi 1/Msi1 Antibody (AAA19172) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Musashi 1/Msi1, Polyclonal Antibody (Cat# AAA19172)

Full Name

Anti-Musashi 1/Msi1 Picoband Antibody

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins

Applications

IHC, WB

Purity

Immunogen affinity purified

Pricing

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IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling DLL3 with (1:25), followed by Dylight 488- conjugated goat anti- rabbit IgG secondary antibody at (1:200) (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OScell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (1:100) (red).)

DLL3, Polyclonal Antibody (Cat# AAA26850)

Full Name

DLL3, CT (DLL3, Delta-like protein 3, Drosophila Delta homolog 3) (APC)

Gene Names

DLL3; SCDO1

Reactivity

Human

Applications

IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of MYLK using anti-MYLK antibody (AAA19154).MYLK was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYLK Antibody (AAA19154) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

MYLK, Polyclonal Antibody (Cat# AAA19154)

Full Name

Anti-MYLK Picoband antibody

Gene Names

MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

EIA, IHC, WB

Pricing

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TMPRSS2, Polyclonal Antibody (Cat# AAA28646)

Full Name

TMPRSS2 Antibody (Center)

Gene Names

TMPRSS2; PP9284; PRSS10

Reactivity

Reactivity: Human, Mouse
Predicted: Mouse

Applications

WB, EIA, FC/FACS

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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WB (Western Blot) (FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src Mab (AAA28639) was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4 degree C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.)

SRC, Monoclonal Antibody (Cat# AAA28639)

Full Name

SRC Antibody

Gene Names

SRC; ASV; SRC1; c-SRC; p60-Src

Reactivity

Human, mouse

Applications

WB, EIA, IF

Purity

This antibody is purified through a protein G column, followed by dialysis against PBS.

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of MED15 using anti-MED15 antibody (AAA19169).MED15 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED15 Antibody (AAA19169) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

MED15/Pcqap, Polyclonal Antibody (Cat# AAA19169)

Full Name

Anti-MED15/Pcqap Picoband Antibody

Gene Names

MED15; TIG1; CAG7A; CTG7A; PCQAP; TIG-1; TNRC7; ARC105

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

EIA, IHC, WB

Purity

Immunogen affinity purified

Pricing

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IF (Immunofluorescence) (AAA31072 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

LKB1, Polyclonal Antibody (Cat# AAA31072)

Full Name

Phospho-LKB1 (Ser428) Antibody

Gene Names

STK11; PJS; LKB1; hLKB1

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

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IGF1R, Polyclonal Antibody (Cat# AAA30993)

Full Name

Phospho-IGF1R (Tyr1346) Antibody

Gene Names

IGF1R; IGFR; CD221; IGFIR; JTK13

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

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Tau, Polyclonal Antibody (Cat# AAA31005)

Full Name

Phospho-Tau (Ser262) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Cleaved-Caspase 1, Polyclonal Antibody (Cat# AAA31336)

Full Name

Cleaved-Caspase 1 (Ala317), p10 Antibody

Gene Names

CASP1; ICE; P45; IL1BC

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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FAK, Polyclonal Antibody (Cat# AAA31066)

Full Name

Phospho-FAK (Tyr576) Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using C-CBL mouse mAb with DAB staining.)

c-Cbl, Monoclonal Antibody (Cat# AAA14105)

Full Name

Anti-c-Cbl

Gene Names

CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

Ascitic Fluid

Pricing

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WB (Western Blot) (Western blot analysis of extracts from Hela and MCF cells using NRF2 Mouse mAb at 1:1000 dilution. Predicted band size: 68KDa. Observed band size: 68kDa.)

NRF2, Monoclonal Antibody (Cat# AAA14162)

Full Name

Anti-NRF2 Mouse mAb

Gene Names

NFE2L2; NRF2; HEBP1

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purified

Pricing

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Chk2, Polyclonal Antibody (Cat# AAA30960)

Full Name

Phospho-Chk2 (Thr68) Antibody

Gene Names

CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

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FCM (Flow Cytometry) (CCR7 Antibody (N-term) (AAA28701) flow cytometric analysis of 293 cells (right histogram) compared to a negative controlcell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

CCR7, Polyclonal Antibody (Cat# AAA28701)

Full Name

CCR7 Antibody (N-term)

Gene Names

CCR7; BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7

Reactivity

Human, mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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MITF, Polyclonal Antibody (Cat# AAA30956)

Full Name

Phospho-MITF (Ser180/73) Antibody

Gene Names

MITF; MI; WS2; CMM8; WS2A; COMMAD; bHLHe32

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Peptide affinity puriification

Pricing

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FCM (Flow Cytometry) (LTF Antibody flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (lefthistogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for theanalysis.)