Search results for " Stem cell"

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eIF2alpha, Antibody (Cat# AAA17981)

Full Name

eIF2alpha (Ab-51) Antibody

Gene Names

EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody (AAA19130).Flt3 / CD135 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Flt3 / CD135 Antibody (AAA19130) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Flt3/CD135, Polyclonal Antibody (Cat# AAA19130)

Full Name

Anti-Flt3/CD135 Picoband Antibody

Reactivity

Mouse, Rat
No cross reactivity with other proteins.

Applications

EIA, IHC, WB

Purity

Immunogen affinity purified

Pricing

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Application Data (Detection limit for recombinant GST tagged NODAL is ~0.3ng/ml as a capture antibody.)

NODAL, Monoclonal Antibody (Cat# AAA25180)

Full Name

NODAL (Nodal Homolog, MGC138230) (FITC)

Gene Names

NODAL; HTX5

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged NODAL is ~0.3ng/ml as a capture antibody.)

NODAL, Monoclonal Antibody (Cat# AAA24883)

Full Name

NODAL (Nodal Homolog, MGC138230) (Biotin)

Gene Names

NODAL; HTX5

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Vimentin, Monoclonal Antibody (Cat# AAA28380)

Full Name

[KO Validated] Vimentin Rabbit mAb

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

Von Willebrand Factor Antigen (VWF:Ag), ELISA Kit (Cat# AAA27452)

Full Name

Sheep Von Willebrand Factor Antigen (VWF:Ag) ELISA Kit

Gene Names

VWF; VWD; F8VWF

Reactivity

Sheep

Pricing

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Src, Antibody (Cat# AAA17990)

Full Name

Src (Phospho-Tyr529) Antibody

Gene Names

SRC; ASV; SRC1; THC6; c-SRC; p60-Src

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

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WB (Western Blot) (WB Suggested Anti-SOX2 antibody Titration: 1 ug/mLSample Type: Human OVCAR-3)

SOX2, Polyclonal Antibody (Cat# AAA23461)

Full Name

SOX2 antibody - middle region

Gene Names

SOX2; ANOP3; MCOPS3

Reactivity

Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

IHC, WB

Purity

Affinity Purified

Pricing

Protein Wnt-7b (WNT7B), Recombinant Protein (Cat# AAA18497)

Full Name

Recombinant Human Protein Wnt-7b (WNT7B)

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

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SCF, Active Protein (Cat# AAA14944)

Full Name

Mouse SCF

Gene Names

Kitl; Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; blz; Kitlg; contrasted

Reactivity

Mouse

Purity

> 95% by SDS-PAGE & silver stain

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using LLGL1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

LLGL1, Polyclonal Antibody (Cat# AAA28385)

Full Name

LLGL1 Rabbit pAb

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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ZNF595, Polyclonal Antibody (Cat# AAA17999)

Full Name

ZNF595 Antibody

Reactivity

Reacts with: Human
Predicted: Rat, Mouse.
Not yet tested in other species.

Applications

EIA, WB, IHC

Pricing

Kv4.2/KCND2, Antibody (Cat# AAA18001)

Full Name

Kv4.2/KCND2 (Phospho-Ser616) Antibody

Gene Names

KCND2; RK5; KV4.2

Reactivity

Human, Mouse, Rat

Applications

IHC, EIA

Pricing

TGF beta Receptor I, Antibody (Cat# AAA18002)

Full Name

TGF beta Receptor I (Phospho-Ser165) Antibody

Gene Names

TGFBR1; AAT5; ALK5; ESS1; LDS1; MSSE; SKR4; TBRI; ALK-5; LDS1A; LDS2A; TBR-i; TGFR-1; ACVRLK4; tbetaR-I

Reactivity

Human, Mouse, Rat

Applications

IF, EIA, FC/FACS

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

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WB (Western Blot) (ZEB2 antibody - middle region validated by WB using H9 human embryonic stem cells at 1:1000.)

ZEB2, Polyclonal Antibody (Cat# AAA23465)

Full Name

ZEB2 antibody - middle region

Gene Names

ZEB2; SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1

Reactivity

Cow, Guinea Pig, Horse, Human, Mouse, Pig, Rat, Zebrafish

Applications

IHC, WB

Purity

Affinity Purified

Pricing

CD133, Antibody (Cat# AAA17982)

Full Name

CD133 Antibody

Gene Names

PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061

Reactivity

Human

Applications

Western Blot

Purity

Affinity Purification

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (AAA19172).Musashi 1/Msi1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Musashi 1/Msi1 Antibody (AAA19172) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Musashi 1/Msi1, Polyclonal Antibody (Cat# AAA19172)

Full Name

Anti-Musashi 1/Msi1 Picoband Antibody

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins

Applications

IHC, WB

Purity

Immunogen affinity purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of Raw264.7 cells using CD44 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

CD44, Polyclonal Antibody (Cat# AAA22201)

Full Name

CD44 Polyclonal Antibody

Gene Names

CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III

Reactivity

Human, Mouse, Rat

Applications

IHC, IF

Purity

Affinity purification

Pricing

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WB (Western Blot) (TRAPPC4 monoclonal antibody. Western Blot analysis of TRAPPC4 expression in NIH/3T3.)

TRAPPC4, Monoclonal Antibody (Cat# AAA24056)

Full Name

TRAPPC4 (Trafficking Protein Particle Complex Subunit 4, Synbindin, TRS23 Homolog, Hematopoietic Stem/Progenitor Cell Protein 172, SBDN, CGI-104, HSPC172, PTD009)

Gene Names

TRAPPC4; SBDN; TRS23; PTD009; CGI-104; HSPC172; SYNBINDIN

Reactivity

Human, Mouse

Applications

EL/EIA, WB, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of HUVEC cells with CD133 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)

CD133, Polyclonal Antibody (Cat# AAA29951)

Full Name

CD133 Antibody

Gene Names

PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061

Reactivity

Human

Applications

WB, ICC, IHC, FC/FACS

Purity

Peptide affinity purified

Pricing

Embryonic Stem MESPU30, ELISA Kit (Cat# AAA17194)

Full Name

Porcine Embryonic Stem MESPU30 ELISA Kit

Reactivity

Porcine

Pricing

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Application Data (Detection limit for recombinant GST tagged NODAL is ~0.3ng/ml as a capture antibody.)

NODAL, Monoclonal Antibody (Cat# AAA24292)

Full Name

NODAL (Nodal Homolog, MGC138230) (AP)

Gene Names

NODAL; HTX5

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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SDS-PAGE (SDS-PAGE Analysis Purified CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R). Confirmation of Purity and Integrity of Antibody.)

CD34, Monoclonal Antibody (Cat# AAA23910)

Full Name

CD34 (Hematopoietic Stem Cell & Endothelial Marker)

Reactivity

Human, Rat. Others not known.

Applications

Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

C-type lectin domain family 11 member A (CLEC11A), ELISA Kit (Cat# AAA27357)

Full Name

Mouse C-type lectin domain family 11 member A (CLEC11A) ELISA Kit

Gene Names

CLEC11A; P47; SCGF; LSLCL; CLECSF3

Reactivity

Mouse

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SOX9 antibody . Western blot was performed from the immunoprecipitate using SOX9 antibody at a dilition of 1:1000.)

SOX9, Monoclonal Antibody (Cat# AAA28384)

Full Name

SOX9 Rabbit mAb

Gene Names

SOX9; CMD1; SRA1; CMPD1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

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WB (Western Blot) (When PSF1 gene was deleted by RNAi, no immunoblot was detected by this mAb, suggesting specificity of this mAb for PSF1)

PSF1, Monoclonal Antibody (Cat# AAA17916)

Full Name

PSF1 antibody

Gene Names

Psf1; CG9187; CG9187-PA; DmelCG9187; psf1

Reactivity

Mouse

Applications

ICC, IHC, WB

Pricing

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WB (Western Blot) (TRAPPC4 monoclonal antibody. Western Blot analysis of TRAPPC4 expression in NIH/3T3.)

TRAPPC4, Monoclonal Antibody (Cat# AAA24983)

Full Name

TRAPPC4 (Trafficking Protein Particle Complex Subunit 4, Synbindin, TRS23 Homolog, Hematopoietic Stem/Progenitor Cell Protein 172, SBDN, CGI-104, HSPC172, PTD009) (Biotin)

Gene Names

TRAPPC4; SBDN; TRS23; PTD009; CGI-104; HSPC172; SYNBINDIN

Reactivity

Human, Mouse

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of rat brain using GFAP Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

GFAP, Monoclonal Antibody (Cat# AAA28378)

Full Name

GFAP Rabbit mAb

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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WB (Western Blot) (TRAPPC4 monoclonal antibody. Western Blot analysis of TRAPPC4 expression in NIH/3T3.)

TRAPPC4, Monoclonal Antibody (Cat# AAA25279)

Full Name

TRAPPC4 (Trafficking Protein Particle Complex Subunit 4, Synbindin, TRS23 Homolog, Hematopoietic Stem/Progenitor Cell Protein 172, SBDN, CGI-104, HSPC172, PTD009) (FITC)

Gene Names

TRAPPC4; SBDN; TRS23; PTD009; CGI-104; HSPC172; SYNBINDIN

Reactivity

Human, Mouse

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Src, Antibody (Cat# AAA17987)

Full Name

Src (Phospho-Tyr418) Antibody

Gene Names

SRC; ASV; SRC1; THC6; c-SRC; p60-Src

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

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Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Cytokeratin 15, Monoclonal Antibody (Cat# AAA23924)

Full Name

Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker)

Gene Names

KRT15; K15; CK15; K1CO

Reactivity

Human

Applications

IF, WB, IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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WB (Western Blot) (WB Suggested Anti-HGF Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 293T cell lysateThere is BioGPS gene expression data showing that HGF is expressed in HEK293T)

HGF, Polyclonal Antibody (Cat# AAA23517)

Full Name

HGF antibody - N-terminal region

Gene Names

HGF; SF; HGFB; HPTA; F-TCF; DFNB39

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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WB (Western Blot) (TRAPPC4 monoclonal antibody. Western Blot analysis of TRAPPC4 expression in NIH/3T3.)

TRAPPC4, Monoclonal Antibody (Cat# AAA25572)

Full Name

TRAPPC4 (Trafficking Protein Particle Complex Subunit 4, Synbindin, TRS23 Homolog, Hematopoietic Stem/Progenitor Cell Protein 172, SBDN, CGI-104, HSPC172, PTD009) (HRP)

Gene Names

TRAPPC4; SBDN; TRS23; PTD009; CGI-104; HSPC172; SYNBINDIN

Reactivity

Human, Mouse

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (WB Suggested Anti-UBE2M Antibody Titration: 0.2-1 ug/mlPositive Control: 721_B cell lysateUBE2M is supported by BioGPS gene expression data to be expressed in 721_B)

UBE2M, Polyclonal Antibody (Cat# AAA23508)

Full Name

UBE2M antibody - N-terminal region

Gene Names

UBE2M; UBC12; hUbc12; UBC-RS2

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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Application Data (Detection limit for recombinant GST tagged NODAL is ~0.3ng/ml as a capture antibody.)

NODAL, Monoclonal Antibody (Cat# AAA25770)

Full Name

NODAL (Nodal Homolog, MGC138230) (PE)

Gene Names

NODAL; HTX5

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Cytokeratin 15, Monoclonal Antibody (Cat# AAA23923)

Full Name

Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker)

Gene Names

KRT15; K15; CK15; K1CO

Reactivity

Human

Applications

FC/FACS, IF, WB, IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

SCF, Active Protein (Cat# AAA14382)

Full Name

SCF protein (Mouse)

Gene Names

KITLG; SF; MGF; SCF; FPH2; FPHH; KL-1; Kitl; SHEP7

Purity

> 98% pure

Pricing

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Lin28a, Recombinant Protein (Cat# AAA14519)

Full Name

Lin28a (full-length) (human), (recombinant)

Gene Names

LIN28A; CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A

Applications

Western Blot

Purity

>95% (SDS-PAGE)
Purified by multi-step chromatography

Pricing

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SDS-PAGE (SDS-PAGE Analysis Purified Podocalyxin Mouse Monoclonal Antibody (3D3). Confirmation of Integrity and Purity of Antibody.)

Podocalyxin (PODXL), Monoclonal Antibody (Cat# AAA13811)

Full Name

Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Mouse Monoclonal Antibody

Gene Names

PODXL; PC; PCLP; Gp200; PCLP-1

Reactivity

Human, Rabbit, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry

Pricing

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SDS-PAGE (SDS-PAGE Analysis Purified EGFR Mouse Monoclonal Antibody (GFR/2596).Confirmation of Integrity and Purity of Antibody)

GFAP, Monoclonal Antibody (Cat# AAA13808)

Full Name

GFAP (Astrocyte & Neural Stem Cell Marker) Mouse Monoclonal Antibody

Gene Names

GFAP; ALXDRD

Reactivity

Human, Mouse, Rat, Cow, Pig, Rabbit, Chicken

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Purity

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry: Human kidney tissue stained with rabbit Anti-CD133 antibody (Cat# AAA14080) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)

CD133, Antibody (Cat# AAA14080)

Full Name

Rabbit anti CD133 antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunoprecipitation

Purity

The Rabbit IgG is purified by Epitope affinity purification

Pricing

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KIT, Polyclonal Antibody (Cat# AAA31006)

Full Name

Phospho-KIT (Tyr703) Antibody

Gene Names

KIT; PBT; SCFR; C-Kit; CD117; MASTC

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

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Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

Application Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12232)

Full Name

RAT ANTI TUBULIN ALPHA:HRP

Applications

Immunohistochemistry, Western Blot

Pricing

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Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

Application Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12009)

Full Name

RAT ANTI TUBULIN ALPHA

Applications

Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western Blot

Pricing

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FCM (Flow Cytometry) (Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using CD31 Mouse Monoclonal Antibody (JC/70A) followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).)