Search results for " Programmed Cell Death"

57 results for " Programmed Cell Death" - showing 50-57

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Standard Curve (Sample)

CD274 molecule, ELISA Kit (Cat# AAA15413)

Full Name

Human Programmed Death Ligand-1, PD-L1/CD274 ELISA Kit

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Reactivity

Human

Pricing

Standard Curve (Sample)

programmed cell death 1, ELISA Kit (Cat# AAA14992)

Full Name

Human Programmed Death 1, PD-1 ELISA Kit

Gene Names

PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1

Reactivity

Human

Pricing

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IF (Immunofluorescence) (Figure 12 Immunohistochemistry Validation of PD-L1in Human Tumors (Gadiot et al., 2011)Immunohistochemical analysis of patient tumors labeling PD-L1 with anti-PD-L1 antibodies (AAA10941). Several anti-PD-L1 antibodies were tested for staining, “Only 1 antibody gave no background staining and was competitively blocked by the addition of PD-L1Fc protein (AAA10941)”.)

IF (Immunofluorescence) (Figure 11 Immunofluorescence Validation of PD-L1 intumors in Human Cells (Dhar et al., 2018)(A) Several antibody brands were first tested with RBCs,WBCs, and HeLa cells. (AAA10941) was chosen as it provided the highest staining intensity. (B, C) Using the optimal conditions of anti-PDL1 at a concentration of 50ug/mL, following by goat anti-rabbit Alexa Fluor 647, PDL-1 staining was tested on several lung cancer cell lines: (adenocarcinoma), adenocarcinoma), (squamous) and WBCs asa control. (D) Once validated, patient samples were stained for PD-L1, CK, CD45, DAPI.)

IF (Immunofluorescence) (Figure 10 Immunofluorescence Validation of PD-L1 in Rat HeartImmunofluorescence analysis of 4% paraformaldehyde-fixed rat heart tissue labeling PD-L1 with AAA10941 at 20 ug/ml,followed by goat anti-rabbit IgG secondary antibody at 1/250 dilution (red).)

IHC (Immunohistchemistry) (Figure 9 Immunohistochemistry Validation of PD-L1 in Human HeartImmunohistochemical analysis of paraffin-embedded human heart tissue using anti-PD-L1 antibody (AAA10941) at 2.5 °g/ml. Tissue was fixed with formalde hyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used assecondary. Counter stained with Hematoxylin.)

IHC (Immunohistochemistry) (Figure 8 Immunohistochemistry Validation of PD-L1 in Rat HeartImmunohistochemical analysis of paraffin-embedded rat heart tissue using anti-PD-L1 antibody (AAA10941) at 5 ug/ml.Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goatanti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin)

FCM (Flow Cytometry) (Figure 7 Flow Cytometry Validation of PD-L1Overlay histogram showing A-20 cells stained with AAA10941 (red line, 1ug/1x106 cells). 1 h incubation at 4°C in 2% FBS/PBS. Followed by secondary antibody 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 1 h 4°C. Isotype control antibody (Green line) was mouse IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10,000 events was performed.)

IF (Immunofluorescence) (Figure 6 Immunofluorescence Validation of PD-L1 in Human HeartImmunofluorescent analysis of 4% paraformaldehyde-fixed human heart tissue labeling PD-L1 with AAA10941 at 20μg/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human heart tissue.)

IHC (Immunohistochemistry) (Figure 5 Immunohistochemistry Validation of PD-L1 in Human Tonsil CellsImmunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-L1 antibody (AAA10941) at 5ug/ml. Tissue was fixed with formalde hyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

WB (Western Blot) (Figure 4 Validation with PD-L1 over expression in 293 cellsLoading: Lysates/proteins at 15 μg per lane. Lane 1: non-transfected 293 cells Lane 2: PD-L1 overexpressed 293 cells Antibodies: AAA10941 (1 μg/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgGHRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 3 Validation with PD-L1 siRNA Knockdown in HeLa CellsHeLa cells were transfected with control siRNAs (lane 1) orPD-L1 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: (2 ug/mL) and GAPDH (, 0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.)

WB (Western Blot) (Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse cell linesLoading: 15 ug of lysates per lane. Antibodies: AAA10941 (2ug/mL), (2 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit and or anti-mouse IgG HRP conjugate at 1:10000 and 1:5000 dilution, respectively.)

WB (Western Blot) (Figure 1 Western Blot Validation of PD-L1 in HeLa CellsLoading: 15ug of lysates per lane. Antibodies: AAA10941 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

PDL-1, Polyclonal Antibody (Cat# AAA10941)

Full Name

PDL-1 Antibody

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

PD-L1 Antibody is affinity chromatography purified via peptide column.

Pricing

WB (Western Blot) (Western blot analysis of lysate from HT-29 cell line, using ERN2 Antibody (N-term). AAA28749 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)

ERN2, Polyclonal Antibody (Cat# AAA28749)

Full Name

ERN2 Antibody (N-term)

Gene Names

ERN2; IRE1b; IRE2p; hIRE2p; IRE1-BETA

Reactivity

Human

Applications

Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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FCM (Flow Cytometry) (Overlay histogram showing Hela cells stained with AAA27016 (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)

FCM (Flow Cytometry) (Overlay histogram showing A549 cells stained with AAA27016 (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)

Application Data (Overlay histogram showing 293 cells stained with AAA27016 (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)

IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with AAA27016 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)

IF (Immunofluorescence) (Immunofluorescence staining of A549 cells with AAA27016 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)

IF (Immunofluorescence) (Immunofluorescence staining of 293 cells with AAA27016 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)

IHC (Immunohistochemistry) (IHC image of AAA27016 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistochemistry) (IHC image of AAA27016 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western BlotPositive WB detected in: Hela whole cell lysateAll lanes: PD-L1 antibody at 1:1000, 1:2000, 1:4000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 34, 21 kDaObserved band size: 55 kDa)

WB (Western Blot) (Western BlotPositive WB detected in: A549 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysateAll lanes: PD-L1 antibody at 1:2500SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 34, 21 kDaObserved band size: 55 kDa)

PD-L1, Monoclonal Antibody (Cat# AAA27016)

Full Name

PD-L1 Monoclonal Antibody

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

>95%, Protein G purified

Pricing

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WB (Western Blot) (Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 2 ug/ml on h Spleen lysate.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of PD-L1 in Human Lung Cancer tissue using PD-L1 antibody (Clone: ABM4E54).)

WB (Western Blot) (Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 0.5 ug/ml on (1) HepG2, (2) SKBR3, (3) A431, (4) THP1, (5) NCCIT, (6) PC3, (7) PANC-1, (8) U87 and (9) KATO-111 lysates.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of PD-L1 in Human Tonsil tissue using PD-L1 antibody (Clone: ABM4E54) at 5 ug/ml.)

WB (Western Blot) (Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 2 ug/ml on (1) A549, (2) MCF-7, (3) 293, (4) HCT-116, (5) Saos2 and (6) Hela lysates.)

WB (Western Blot) (Western blot analysis of PD-L1. Anti-PD-L1 antibody (Clone: ABM4E54) was tested at 0.5 ug/ml on Recombinat lysates.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of PD-L1 in Hodkin's Lymphoma tissue using PDL1 antibody (Clone: ABM4E54) at 5 ug/ml.)

FCM (Flow Cytometry) (Cell Surface FLOW analysis of PD-L1 in PHA treated human PBMC using 1 ug of PD-L1 antibody (Clone:ABM4E54). Green represents isotype control; red represents anti-PD-L1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)

PD-L1, Monoclonal Antibody (Cat# AAA14873)

Full Name

Monoclonal Antibody to PD-L1 (Clone: ABM4E54)

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry, Western Blot

Purity

Purified
Protein G Chromatography

Pricing

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WB (Western Blot)

IHC (Immunohistochemistry)

IHC (Immunohistchemistry)

IHC (Immunohistochemistry)

IF (Immunofluorescence)

FCM (Flow Cytometry)

ALIX/PDCD6IP, Monoclonal Antibody (Cat# AAA19212)

Full Name

Anti-ALIX/PDCD6IP Antibody (Monoclonal, 14D10)

Gene Names

PDCD6IP; AIP1; ALIX; HP95; DRIP4

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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57 results for " Programmed Cell Death" - showing 50-57

CD274 molecule, ELISA Kit (Cat# AAA15413)

programmed cell death 1, ELISA Kit (Cat# AAA14992)

PDL-1, Polyclonal Antibody (Cat# AAA10941)

ERN2, Polyclonal Antibody (Cat# AAA28749)

PD-L1, Monoclonal Antibody (Cat# AAA27016)

PD-L1, Monoclonal Antibody (Cat# AAA14873)

ALIX/PDCD6IP, Monoclonal Antibody (Cat# AAA19212)

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57 results for " Programmed Cell Death" - showing 50-57

CD274 molecule, ELISA Kit (Cat# AAA15413)

programmed cell death 1, ELISA Kit (Cat# AAA14992)

PDL-1, Polyclonal Antibody (Cat# AAA10941)

ERN2, Polyclonal Antibody (Cat# AAA28749)

PD-L1, Monoclonal Antibody (Cat# AAA27016)

PD-L1, Monoclonal Antibody (Cat# AAA14873)

ALIX/PDCD6IP, Monoclonal Antibody (Cat# AAA19212)

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