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Type
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Gene Name
Isotype
Host
Application
Clone
10000 results for " Cell" - showing 9700-9750
Application Data
(Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)
TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12009)
Full Name
RAT ANTI TUBULIN ALPHA
Applications
Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western Blot
Pricing
Application Data
(Published customer image: Effect of clustered ephrin-A1-Fc on vascular formation in the rat striatum. Clustered ephrin-A1-Fc was injected into the lesioned side of the lateral ventricle in the unilaterally lesioned rats. Brains taken 6 weeks after injection were sectioned coronally and stained for GFAP (green) and RECA-1 (red) and with DAPI (nuclei; blue). The rectangular insets are shown in Fig. 8B. Scale bar: 100 um.From: Jing X, Miwa H, Sawada T, Nakanishi I, Kondo T, et al. (2012) Ephrin-A1-Mediated Dopaminergic Neurogenesis and Angiogenesis in a Rat Model of Parkinson's Disease. PLoS ONE 7(2): e32019.)
RECA-1, Monoclonal Antibody (Cat# AAA12018)
Full Name
MOUSE ANTI RAT RECA-1
Applications
Immunohistochemistry, Immunofluorescence
Pricing
Application Data
(Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detaction of aromatase in human tissues by Immunohistochemistry on paraffin sectionsImage caption:Morphology and P450 arom immunoreactivity of tumoral region in human testis with seminoma. A-B: Haematoxylin-eosin staining. C-D: Strong P450 arom immunoreactivity in cytoplasm of neoplastic cells (Nc) and unstained lymphocytes (L). Insert: absorption control. Scale bars: A, 20 um; B-C, 12.5 um; D, 5 um.From: Rago V, Romeo F, Aquila S, Montanaro D, And S, Carpino A. Cytochrome P450 aromatase expression in human seminoma. Reprod Biol Endocrinol. 2005 Dec 22;3:72.)
CYTOCHROME P450 AROMATASE, Monoclonal Antibody (Cat# AAA12113)
Full Name
MOUSE ANTI HUMAN CYTOCHROME P450 AROMATASE
Gene Names
CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12081)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
Western Blot
Pricing
Api m 1, Recombinant Protein (Cat# AAA20033)
Full Name
rApi m 1 (Apis mellifera 1.0101)
Applications
ELISA
Purity
Purified by sequential steps of ion exchange and affinity chromatography.
Purified Azide Free
Purified Azide Free
Pricing
WB (Western Blot)
(OMP Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the OMP antibody detected the OMP protein (arrow).)
OMP, Polyclonal Antibody (Cat# AAA28786)
Full Name
OMP Antibody (N-term)
Reactivity
Human
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data
(Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.)
FGF21, Polyclonal Antibody (Cat# AAA23563)
Full Name
FGF21 antibody - N-terminal region
Reactivity
Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: A549) using a 12 % SDS PAGE gel and NKG2D antibody at a dilution of 1:1000)
NKG2D, Polyclonal Antibody (Cat# AAA17934)
Full Name
NKG2D antibody
Applications
Immunohistochemistry, Western Blot
Purity
NKG2D antibody was purified by antigen-affinity chromatography
Pricing
Application Data
(ELISA plate was coated by N protein, 100 uL/cell, at various concentrations. The direct ELISA analysis was performed by loading 100 uL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hours at 37 degree C, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.)
COVID 19 Nucleocapsid (NP) Coronavirus, Recombinant Protein (Cat# AAA27948)
Full Name
2019-nCov Nucleocapsid Recombinant Protein (full length)
Applications
ELISA
Purity
Purity: >95% by SDS-page
Purification: Ni column
Purification: Ni column
Pricing
WB (Western Blot)
(STK33 monoclonal antibody, Western Blot analysis of STK33 expression in HeLa.)
STK33, Monoclonal Antibody (Cat# AAA25856)
Full Name
STK33 (Serine/Threonine Kinase 33) (PE)
Reactivity
Human
Applications
Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GPC3 mouse mAb with DAB staining.)
GPC3, Monoclonal Antibody (Cat# AAA14118)
Full Name
Anti-GPC3 Mouse mAb
Gene Names
GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Cyclin Dependent Kinase Inhibitor 2A (CDKN2A), Polyclonal Antibody (Cat# AAA20299)
Full Name
Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
Westen Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
Application Data
(Staining of mouse spleen cells with Rat anti Mouse CD3 Epsilon (T3): APC)
CD3, Monoclonal Antibody (Cat# AAA11984)
Full Name
RAT ANTI MOUSE CD3
Gene Names
Cd3e; CD3; T3e; AI504783; CD3epsilon
Applications
Immunohistochemistry, Flow Cytometry
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using PP2A-B56δ/PR61δ/PP2A-B56δ/PR61δ/PPP2R5D antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
PP2A-B56delta/PR61delta/PPP2R5D, Polyclonal Antibody (Cat# AAA28319)
Full Name
PP2A-B56delta/PR61delta/PPP2R5D Rabbit pAb
Gene Names
PPP2R5D; B56D
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(AAA31106 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
GPR141, Polyclonal Antibody (Cat# AAA31106)
Full Name
GPR141 Antibody
Gene Names
GPR141; PGR13
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
NFkappaB p65, Monoclonal Antibody (Cat# AAA29642)
Full Name
NFkappaB p65 Mouse Monoclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification using immunogen.
Pricing
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with VEGF Monoclonal Antibody (SPM225).)
VEGF (Vascular Endothelial Growth Factor), Monoclonal Antibody (Cat# AAA13827)
Full Name
VEGF (Vascular Endothelial Growth Factor) Mouse Monoclonal Antibody
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human, Mouse, Rat, Rabbit, Dog. Others not known.
Applications
Immunofluorescence, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(AAA31121 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
FABP4, Polyclonal Antibody (Cat# AAA31121)
Full Name
FABP4 Antibody
Gene Names
FABP4; aP2; ALBP; AFABP; A-FABP; HEL-S-104
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IF (Immunofluorescence)
(AAA13648 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
AKAP9/AKAP450/CG-NAP, Polyclonal Antibody (Cat# AAA13648)
Full Name
Goat anti-AKAP9/AKAP450/CG-NAP Antibody
Gene Names
AKAP9; LQT11; PRKA9; AKAP-9; CG-NAP; YOTIAO; AKAP350; AKAP450; PPP1R45; HYPERION; MU-RMS-40.16A
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse
Applications
Peptide ELISA, Western Blot, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27698)
Full Name
VPS35 Antibody, Clone 10A8: PerCP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
FCM (Flow Cytometry)
(Figure 1. Elisa plate pre-coated by 2 ug/ml (100ul/well) SARS-CoV-2 RBD protein can bind Rabbit Anti-SARS-CoV-2 RBD monoclonal antibody ( clone:DM35) in a linear range of 0.19-200 ng/ml.)
COVID 19 Spike RBD (DM35) Coronavirus, Monoclonal Antibody (Cat# AAA11707)
Full Name
Anti-SARS-CoV-2 RBD antibody (DM35), Rabbit mAb
Reactivity
SARS-CoV-2
Applications
Flow Cytometry
Purity
Purified from cell culture supernatant by affinity chromatography
Pricing
FCM (Flow Cytometry)
(Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using CD31 Mouse Monoclonal Antibody (JC/70A) followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).)
CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA13818)
Full Name
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human, Cynomolgus Monkey, Rabbit.
Does not react with Rat and Pig
Does not react with Rat and Pig
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (AAA11664).Flotillin 2 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Flotillin 2 Antibody (AAA11664) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Flotillin 2, Polyclonal Antibody (Cat# AAA11664)
Full Name
Anti-Flotillin 2 Antibody
Gene Names
FLOT2; ESA; ECS1; ESA1; ECS-1; M17S1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Ntera-2 cells stained with CSB-MA018403A0m (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
OCT4, Monoclonal Antibody (Cat# AAA27015)
Full Name
OCT4 Monoclonal Antibody
Gene Names
POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
>95%
Protein G Purified
Protein G Purified
Pricing
IHC-P (Immunohistochemistry-Paraffin)
(At 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediatedantigen retrieval step in citrate buffer was performed. Thetissue was then blocked and incubated with the antibody for1.5 hours at 22°C. An HRP conjugated goat anti-rabbitantibody was used as the secondary antibody.)
c-Kit, Polyclonal Antibody (Cat# AAA31007)
Full Name
Phospho-c-Kit (Tyr721) Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117; MASTC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
Nucleolin, Monoclonal Antibody (Cat# AAA13823)
Full Name
Nucleolin (Marker of Human Cells) Mouse Monoclonal Antibody
Gene Names
NCL; C23
Reactivity
Human.
Does not react with Mouse, Rat and Cow
Does not react with Mouse, Rat and Cow
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(AAA31051 at 1/200 staining Human bladder cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
SYK, Polyclonal Antibody (Cat# AAA31051)
Full Name
Phospho-SYK (Tyr348) Antibody
Gene Names
SYK; p72-Syk
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(AAA31020 staining HepG2 cells(30min of 4uM Forskolin treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31020) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue))
VEGFR1, Polyclonal Antibody (Cat# AAA31020)
Full Name
Phospho-VEGFR1 (Tyr1213) Antibody
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(HHLA2 Antibody (N-term) western blot analysis in MDA-MB453,CEM,HL-60,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HHLA2 antibody detected the HHLA2 protein (arrow).)
HHLA2, Polyclonal Antibody (Cat# AAA28722)
Full Name
HHLA2 Antibody (N-term)
Gene Names
HHLA2; B7H7; B7-H7
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using AAA28677 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
ATG7, Polyclonal Antibody (Cat# AAA28677)
Full Name
ATG7 Antibody (C-term)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human, Mouse
Predicted reactivity: Chicken, Rat
Predicted reactivity: Chicken, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(Fig-5: Cell Surface staining of human ACE2 in the ACE2/HEK293 stable cell line. ATTO 488-conjugated anti-hACE2 antibody (clone AC18F; was used at 1ug/ 1x10^6 cells (Red). ATTO 488-conjugated mouse IgG1 was used as isotype control at 1ug/ 1x10^6 cells (Green).)
ACE2, Cell Line (Cat# AAA14888)
Full Name
ACE2/HEK293 Stable Cell Line
Gene Names
ACE2; ACEH
Applications
Functional Assay
Pricing
WB (Western Blot)
(Western blot analysis of p53 phosphorylation expression in Etoposide treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
p53, Polyclonal Antibody (Cat# AAA30976)
Full Name
Phospho-p53 (Ser392) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse Beta-glucan Receptor: FITC)
DECTIN-1, Monoclonal Antibody (Cat# AAA12137)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
WB (Western Blot)
(TGFBR2 Antibody western blot of mouse lung tissue lysates (35 ug/lane). The TGFBR2 antibody detected the TGFBR2 protein (arrow).)
TGFBR2, Polyclonal Antibody (Cat# AAA21269)
Full Name
Anti-TGFBR2 Antibody (aa13-40) IHC-plus
Gene Names
TGFBR2; AAT3; FAA3; LDS2; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TGFR-2; TGFbeta-RII
Reactivity
Mouse, Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Protein A purified
Pricing
Application Data
(Mouse anti Human CD14 antibody, clone Tük4 used to identify bovine monocytes in the skin and subcutaneous tissue of adjuvant injected calves by immunofluorescence.Image caption:Cellular recruitment to skin and subcutaneous tissues. (A) HE stained sections of skin with subcutaneous tissue from the side injected with adjuvant and the contralateral side, at 24 h post-injection. Scale bars: 200 ?m. (B) Enlargement of outlined areas in A, as indicated. Scale bars: 20 ?m. (C) Immunofluorescent labeling of subcutaneous tissue on the injected side with antibody against CD14 (green). Scale bar: 20 ?m.From: Lund H, Boysen P, Åkesson CP, Lewandowska-Sabat AM and Storset AK (2016)Transient Migration of Large Numbers of CD14++ CD16+ Monocytes to the Draining Lymph Node after Onset of Inflammation.Front. Immunol. 7:322.This is from an open access article distributed under the terms of the Creative Commons Attribution License.)
CD14, Monoclonal Antibody (Cat# AAA12266)
Full Name
Mouse Anti Human CD14: Amethyst Orange
Reactivity
Human
Applications
Flow Cytometry
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12095)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:FITC
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12096)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:RPE
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 488)
CD81, Monoclonal Antibody (Cat# AAA11869)
Full Name
HAMSTER ANTI MOUSE CD81:FITC
Gene Names
Cd81; Tapa1; Tapa-1; Tspan28
Applications
Flow Cytometry
Pricing
Application Data
(PE conjugatedMouse anti Human CD169 antibody, clone 7-239 used to block CD169 function on myeloid cells.Image caption:Siglec-1 mediates HIV-1 uptake into a storage compartment and enhances HIV-1 trans-infection specially in IFN?-treated monocytes and DCs. A. Uptake of HIV-1NL4–3 by different myeloid cells exposed to IFN?. Cells were cultured with HIV-1 to measure p24Gag by ELISA. Mean values and SEM from four experiments include cells from 12 donors. B. Fold change in HIV-1NL4–3 uptake of cells treated with bafilomycin A1 compared to untreated cells. Mean values and SEM include cells from three donors. C. Relative uptake of HIV-1NL4–3 by IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 uptake by mock-treated cells (set at 100%). Mean values and SEM from two experiments include cells from six donors. D. Confocal microscopy analysis of different IFN?-treated myeloid cells pulsed with HIV-1Cherry and stained for Siglec-1 (Alexa 488), HLA-DR (Alexa 647) and DAPI. (Top) Representative viral pattern for each kind of myeloid cell analyzed, showing maximum fluorescence intensity of four channels. (Bottom) Percentage of myeloid cells with distinct viral patterns: random distribution, polarized accumulation, and sac-like compartment formation, as illustrated in the left drawing. Mean values of 50 cells from two different donors are shown. E. HIV-1 transmission from IFN?-treated myeloid cells to a luciferase reporter CD4+ cell line. HIV-1 infection was determined by induced luciferase activity in relative light units (RLUs). Mean values and SEM from four experiments include cells from 12 donors. F. Relative HIV-1 transmission from IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 trans-infected by mock-treated cells. Mean values and SEM from two experiments include cells from six donors. Statistical differences were assessed with a paired t test in A and E, and with a one sample t-test in B, C and F.From: Pino M, Erkizia I, Benet S, Erikson E, Fernández-Figueras MT, Guerrero D, Dalmau J, Ouchi D, Rausell A, Ciuffi A, Keppler OT, Telenti A, Kräusslich HG, Martinez-Picado J, Izquierdo-Useros N.HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells.Retrovirology. 2015 May 7;12:37.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
CD169, Monoclonal Antibody (Cat# AAA12265)
Full Name
Mouse Anti Human CD169: RPE
Gene Names
SIGLEC1; SN; CD169; SIGLEC-1
Reactivity
Human
Applications
Flow Cytometry
Purity
>95% by SDS PAGE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
Application Data
(Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 488)
CD81, Monoclonal Antibody (Cat# AAA12033)
Full Name
HAMSTER ANTI MOUSE CD81:RPE
Gene Names
Cd81; Tapa1; Tapa-1; Tspan28
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Effect of clustered ephrin-A1-Fc on vascular formation in the rat striatum. Clustered ephrin-A1-Fc was injected into the lesioned side of the lateral ventricle in the unilaterally lesioned rats. Brains taken 6 weeks after injection were sectioned coronally and stained for GFAP (green) and RECA-1 (red) and with DAPI (nuclei; blue). The rectangular insets are shown in Fig. 8B. Scale bar: 100 um.From: Jing X, Miwa H, Sawada T, Nakanishi I, Kondo T, et al. (2012) Ephrin-A1-Mediated Dopaminergic Neurogenesis and Angiogenesis in a Rat Model of Parkinson's Disease. PLoS ONE 7(2): e32019.)
RECA-1, Monoclonal Antibody (Cat# AAA12019)
Full Name
MOUSE ANTI RAT RECA-1
Applications
Immunohistochemistry, Immunofluorescence
Pricing
Application Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . Low power)
CD163, Monoclonal Antibody (Cat# AAA11943)
Full Name
MOUSE ANTI HUMAN CD163
Gene Names
CD163; M130; MM130
Reactivity
Guinea Pig, Pig, Rhesus Monkey, Sheep
Applications
Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoassay, Western Blot
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC AAA12191)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12191)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
Flow Cytometry
Pricing
WB (Western Blot)
NEDD4, Polyclonal Antibody (Cat# AAA14401)
Full Name
NEDD4 Antibody Affinity Purified
Gene Names
Nedd4; Nedd4a
Reactivity
Mouse, Rat
Applications
Western Blot
Pricing
WB (Western Blot)
(Western blot analysis MT-ND6 using HepG2 whole cell lysates)
MT-ND6, Antibody (Cat# AAA27922)
Full Name
MT-ND6 Antibody
Gene Names
MT-ND6; MTND6; ND6
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
PAI-1, Monoclonal Antibody (Cat# AAA27728)
Full Name
Anti-PAI-1 Antibody, Mouse Monoclonal
Gene Names
SERPINE1; PAI; PAI1; PAI-1; PLANH1
Applications
ELISA
Purity
Protein A affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using CAD antibody (AAA28198) at dilution of 1:100. Blue: DAPI for nuclear staining.)
CAD, Polyclonal Antibody (Cat# AAA28198)
Full Name
CAD Polyclonal Antibody
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Host: RabbitTarget Name: OMPSample Tissue: Human U937 Whole CellAntibody Dilution: 5ug/ml)
OMP, Polyclonal Antibody (Cat# AAA23571)
Full Name
OMP antibody - middle region
Reactivity
Tested Reactivity: Human, Rat
Predicted Species Reactivity: Goat, Human, Mouse, Rat
Predicted Species Reactivity: Goat, Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purified
Pricing
Cyfra-21-1, Cytokeratin 19 Fragment Calibrator Grade (Low Cross Reactivity), Native Protein (Cat# AAA13416)
Full Name
Cyfra-21-1 Antigen
Applications
Chemiluminescent Immunoassay
Purity
Ion-Exchange Chromatography
Product is 0.2 um filtered.
Product is 0.2 um filtered.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using TAS1R3 antibody (AAA28219) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1s.)
TAS1R3, Polyclonal Antibody (Cat# AAA28219)
Full Name
TAS1R3 Polyclonal Antibody
Gene Names
TAS1R3; T1R3
Applications
Western Blot
Purity
Affinity purification
Pricing