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Application
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1005 results for " HRP conjugated" - showing 900-950
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: IGSF16 knockout HepG2 cell lysate; Predicted MW: 25kd Observed MW: 37kd Primary Ab: 2ug/ml Rabbit Anti-Human IGSF16 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Immunoglobulin Superfamily, Member 16, Polyclonal Antibody (Cat# AAA21021)
Full Name
Immunoglobulin Superfamily, Member 16 (IGSF16) Polyclonal Antibody
Gene Names
CD300C; LIR; CLM-6; CMRF35; IGSF16; CMRF-35; CMRF35A; CMRF-35A; CMRF35A1; CMRF35-A1
Reactivity
Human, Rat, Pig
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
Affinity Chromatography
Pricing
WB (Western Blot)
(Western blot analysis of lysate from mouse heart tissue lysate, using MYBPC3 Antibody (N-term). AAA28794 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
MYBPC3, Polyclonal Antibody (Cat# AAA28794)
Full Name
MYBPC3 Antibody (N-term)
Gene Names
MYBPC3; FHC; CMH4; CMD1MM; LVNC10; MYBP-C
Reactivity
Human, Mouse, Rat
(Predicted Reactivity: Rat)
(Predicted Reactivity: Rat)
Applications
Immunohistochemistry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(AAA31265 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31265) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
PPP2R5C, Polyclonal Antibody (Cat# AAA31265)
Full Name
PPP2R5C Antibody
Gene Names
PPP2R5C; B56G; PR61G; B56gamma
Reactivity
Human, Rat
Predicted Reactivity: Rabbit(88%)
Predicted Reactivity: Rabbit(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(AAA31059 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Smad2/3, Polyclonal Antibody (Cat# AAA31059)
Full Name
Phospho-Smad2/3 (Thr8) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide affinity columns
Pricing
IHC (Immunohistochemistry)
(AAA30948 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk1, Polyclonal Antibody (Cat# AAA30948)
Full Name
Phospho-Chk1 (Ser317) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
FCM (Flow Cytometry)
(RSPO2 Antibody (C-term) (AAA28708) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
RSPO2, Polyclonal Antibody (Cat# AAA28708)
Full Name
RSPO2 Antibody (C-term)
Gene Names
RSPO2; CRISTIN2
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Westen Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(AAA31053 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
GATA4, Polyclonal Antibody (Cat# AAA31053)
Full Name
Phospho-GATA4 (Ser262) Antibody
Gene Names
GATA4; TOF; ASD2; VSD1; TACHD
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of XIAP phosphorylation expression in Anisomycin treated HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
XIAP, Polyclonal Antibody (Cat# AAA31060)
Full Name
Phospho-XIAP (Ser87) Antibody
Gene Names
XIAP; API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30995 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
BCL-2, Polyclonal Antibody (Cat# AAA30995)
Full Name
Phospho-BCL-2 (Ser70) Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(staining A549 cells cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Cox2, Polyclonal Antibody (Cat# AAA31143)
Full Name
Cox2 Antibody
Gene Names
PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-HDAC3 (Ser424) Antibody expression in mouse lung and mouse brain tissues lysates.The lane on the right is treated with the antigen-specific peptide.)
HDAC3, Polyclonal Antibody (Cat# AAA30951)
Full Name
Phospho-HDAC3 (Ser424) Antibody
Gene Names
HDAC3; HD3; RPD3; RPD3-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Tau, Polyclonal Antibody (Cat# AAA31002)
Full Name
Phospho-Tau (Thr231) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho- peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of LoVo cell lysate, using PIBF1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
PIBF1, Polyclonal Antibody (Cat# AAA31130)
Full Name
PIBF1 Antibody
Gene Names
PIBF1; PIBF; CEP90; JBTS33; C13orf24
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-Tau (Ser214) expression in various lysates)
Tau, Polyclonal Antibody (Cat# AAA30996)
Full Name
Phospho-Tau (Ser214) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30934 at 1/100 staining human TB tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
I kappaB alpha, Polyclonal Antibody (Cat# AAA30934)
Full Name
Phospho-I kappaB alpha (Ser32/Ser36) Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PAR4, Polyclonal Antibody (Cat# AAA31375)
Full Name
Phospho-PAR4 (Thr163) Antibody
Gene Names
PAWR; PAR4; Par-4
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Chk2 phosphorylation expression in Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Chk2, Polyclonal Antibody (Cat# AAA30957)
Full Name
Phospho-Chk2 (Thr387) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U20S cells using anti-Calpastatin antibody (AAA11679).Overlay histogram showing U20S cells stained with AAA11679 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calpastatin Antibody (AAA11679,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Calpastatin, Polyclonal Antibody (Cat# AAA11679)
Full Name
Anti-Calpastatin Antibody
Gene Names
CAST; BS-17; PLACK
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U251 cells using anti-PNP antibody (AAA11680).Overlay histogram showing U251 cells stained with AAA11680 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNP Antibody (AAA11680,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PNP, Polyclonal Antibody (Cat# AAA11680)
Full Name
Anti-PNP Antibody
Gene Names
PNP; NP; PUNP; PRO1837
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 7. IF analysis of CD68 using anti- CD68 antibody CD68 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti- CD68 Antibody overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
CD68, Polyclonal Antibody (Cat# AAA11512)
Full Name
Anti-CD68 antibody
Gene Names
Cd68; Lamp4; gp110; Scard1
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of p53 phosphorylation expression in HU treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
p53, Polyclonal Antibody (Cat# AAA30977)
Full Name
Phospho-p53 (Ser15) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
anti-Cotton Rat IgG h+l, Polyclonal Antibody (Cat# AAA14444)
Full Name
HRP Conjugated Chicken anti-Cotton Rat IgG h+l
Applications
Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Immunofluorescence (IFC) analysis of PC3 cells using AAA14697 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Ribonuclease inhibitor 1, Monoclonal Antibody (Cat# AAA14697)
Full Name
Ribonuclease inhibitor 1 (RNH1)
Gene Names
RNH1; RAI; RNH; MGC4569; MGC18200; MGC54054
Applications
Immunofluorescence, Western Blot
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
SDS-PAGE
(Sample (30 ug of whole cell lysate)A: HelaB: JurKat5% SDS PAGEPrimary antibody diluted at 1: 1000)
LTBP-4, Polyclonal Antibody (Cat# AAA29648)
Full Name
LTBP-4 antibody
Gene Names
LTBP4; ARCL1C; LTBP-4; LTBP4L; LTBP4S
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
Antigen-affinity purification
Pricing
Alanine Aminotransferase (ALT), Polyclonal Antibody (Cat# AAA20296)
Full Name
Polyclonal Antibody to Alanine Aminotransferase (ALT)
Gene Names
Gpt; ALT; ALT1; Gpt1; Gpt-1; 1300007J06Rik; 2310022B03Rik
Reactivity
Mouse
Applications
Westen Blot, Immunohistochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IHC (Immunohistochemistry)
(AAA30941 at 1/100 staining Human lung cancer by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
AC-FKHR, Polyclonal Antibody (Cat# AAA30941)
Full Name
AC-FKHR(K294) Antibody
Gene Names
FOXO1; FKH1; FKHR; FOXO1A
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Cytokeratin 20 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Cytokeratin 20, Polyclonal Antibody (Cat# AAA27786)
Full Name
Anti-Cytokeratin 20 Antibody
Gene Names
KRT20; K20; CD20; CK20; CK-20; KRT21
Reactivity
Human, Mouse, Rat, Bovine, Pig
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(AAA30955 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
JAK2, Polyclonal Antibody (Cat# AAA30955)
Full Name
Phospho-JAK2 (Tyr931) Antibody
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31211 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31211 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
SEPT4, Polyclonal Antibody (Cat# AAA31211)
Full Name
SEPT4 Antibody
Gene Names
SEPTIN4; H5; ARTS; MART; SEP4; CE5B3; SEPT4; PNUTL2; hucep-7; BRADEION; C17orf47; hCDCREL-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
Application Data
(Overlay histogram showing U-2OS cells stained with AP10510c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated n 2% bovine serum albumin to block non-specificprotein-protein interactions followed by the antibody (AP10510c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used wasGoat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events wasperformed)
CF150, Polyclonal Antibody (Cat# AAA28789)
Full Name
CF150 Antibody (Center)
Gene Names
MB21D1; cGAS; h-cGAS; C6orf150
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(AAA31046 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
STAT6, Polyclonal Antibody (Cat# AAA31046)
Full Name
Phospho-STAT6 (Thr645) Antibody
Gene Names
STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using Phospho-PDK1 (Ser241) Antibody.)
PDK1, Polyclonal Antibody (Cat# AAA30952)
Full Name
Phospho-PDK1 (Ser241) Antibody
Gene Names
PDPK1; PDK1; PDPK2; PDPK2P; PRO0461
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30939 at 1/100 staining rat appendiceal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk1, Polyclonal Antibody (Cat# AAA30939)
Full Name
Phospho-Chk1 (Ser280) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31015 at 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
CREB, Polyclonal Antibody (Cat# AAA31015)
Full Name
Phospho-CREB (Ser142) Antibody
Gene Names
CREB1; CREB; CREB-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30966 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Myc, Polyclonal Antibody (Cat# AAA30966)
Full Name
Phospho-Myc (Thr58) Antibody
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing U87 cells stained with AAA28063 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
CD9, Monoclonal Antibody (Cat# AAA28063)
Full Name
CD9 Monoclonal Antibody
Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
>95%, Protein G purified
Pricing
IF (Immunofluorescence)
(AAA30990 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Lyn, Polyclonal Antibody (Cat# AAA30990)
Full Name
Phospho-Lyn (Tyr508) Antibody
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HL-60 cells using anti-METTL3 antibody (AAA19247).Overlay histogram showing HL-60 cells stained with AAA19247 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-METTL3 Antibody (AAA19247, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
METTL3, Polyclonal Antibody (Cat# AAA19247)
Full Name
Anti-METTL3 Antibody
Gene Names
METTL3; M6A; IME4; Spo8; MT-A70
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(AAA30924 staining MCF-7 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Ki67, Polyclonal Antibody (Cat# AAA30924)
Full Name
Ki67 Antibody
Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientifiv).
Pricing
IHC (Immunohistochemistry)
(AAA30967 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Lamin A/C, Polyclonal Antibody (Cat# AAA30967)
Full Name
Phospho-Lamin A/C (Ser392) Antibody
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H3, Polyclonal Antibody (Cat# AAA31353)
Full Name
Acetyl-Histone H3 (Lys122) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%)
Predicted Reactivity: Bovine (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of Collagen 2 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Collagen 2 alpha 1, Polyclonal Antibody (Cat# AAA27787)
Full Name
Anti-Collagen 2 alpha 1 Antibody
Gene Names
COL2A1; AOM; ANFH; SEDC; STL1; COL11A3
Reactivity
Human, Mouse, Rat, Bovine, Dog, Porcine, Rabbit
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Ryanodine Receptor 2 (phospho-Ser2808) staining in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Ryanodine Receptor 2 (phospho-Ser2808), Polyclonal Antibody (Cat# AAA29761)
Full Name
Ryanodine Receptor 2 (phospho-Ser2808) Antibody
Gene Names
RYR2; RyR; ARVC2; ARVD2; RYR-2; VTSIP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Immunogen affinity chromatography
Pricing
WB (Western Blot)
(Western blot analysis of c-Jun phosphorylation expression in UV treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
c-Jun, Polyclonal Antibody (Cat# AAA30983)
Full Name
Phospho-c-Jun (Thr93) Antibody
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of c-Jun phosphorylation expression in UV treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
c-Jun, Polyclonal Antibody (Cat# AAA30984)
Full Name
Phospho-c-Jun (Tyr170) Antibody
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of c-Jun phosphorylation expression in UV treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
c-Jun, Polyclonal Antibody (Cat# AAA30982)
Full Name
Phospho-c-Jun (Thr239) Antibody
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of c-Jun phosphorylation expression in UV treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
c-Jun, Polyclonal Antibody (Cat# AAA30981)
Full Name
Phospho-c-Jun (Ser243) Antibody
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31055 at 1/200 staining Mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
LIMK1, Polyclonal Antibody (Cat# AAA31055)
Full Name
Phospho-LIMK1 (Thr508) Antibody
Gene Names
LIMK1; LIMK; LIMK-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(AAA30994 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IGF1R, Polyclonal Antibody (Cat# AAA30994)
Full Name
Phospho-IGF1R (Tyr1161) Antibody
Gene Names
IGF1R; IGFR; CD221; IGFIR; JTK13
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-Tau (Ser404) expression in various lysates)
Tau, Polyclonal Antibody (Cat# AAA30999)
Full Name
Phospho-Tau (Ser404) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing