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Application
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2304 results for " secondary" - showing 850-900
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using CDH16 antibody at dilution of 1:100 (40x lens).)
CDH16, Polyclonal Antibody (Cat# AAA28211)
Full Name
CDH16 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
Application Data
(Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)
EMR1, Monoclonal Antibody (Cat# AAA26749)
Full Name
EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (FITC)
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse liver using POP4 antibody at dilution of 1:100 (40x lens).)
POP4, Polyclonal Antibody (Cat# AAA28182)
Full Name
POP4 Polyclonal Antibody
Gene Names
POP4; RPP29
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using ANXA8L2 antibody. Blue: DAPI for nuclear staining.)
ANXA8L1, Polyclonal Antibody (Cat# AAA28204)
Full Name
ANXA8L1 Polyclonal Antibody
Gene Names
ANXA8; ANX8; CH17-360D5.2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
Application Data
(Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)
EMR1, Monoclonal Antibody (Cat# AAA26772)
Full Name
EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (PE)
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using CHAF1B antibody. Blue: DAPI for nuclear staining.)
CHAF1B, Polyclonal Antibody (Cat# AAA28173)
Full Name
CHAF1B Polyclonal Antibody
Gene Names
CHAF1B; CAF1; MPP7; CAF-1; CAF1A; CAF1P60; CAF-IP60; MPHOSPH7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using SOX5 antibody. Blue: DAPI for nuclear staining.)
SOX5, Polyclonal Antibody (Cat# AAA10779)
Full Name
SOX5 Polyclonal Antibody
Gene Names
SOX5; L-SOX5; L-SOX5B; L-SOX5F
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of 293T cells with Syntaxin 3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Syntaxin 3, Monoclonal Antibody (Cat# AAA30530)
Full Name
Syntaxin 3 Antibody
Gene Names
STX3; STX3A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of A549 cells with Nucleostemin antibody at 1/50 dilution (Fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Nucleostemin, Monoclonal Antibody (Cat# AAA30502)
Full Name
Nucleostemin Antibody
Gene Names
GNL3; NS; E2IG3; NNP47; C77032
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with DDIT3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
DDIT3, Monoclonal Antibody (Cat# AAA30300)
Full Name
DDIT3 Antibody
Gene Names
DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Bax antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
BAX, Monoclonal Antibody (Cat# AAA30009)
Full Name
BAX Antibody
Gene Names
BAX; BCL2L4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with CD11a antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
CD11a, Monoclonal Antibody (Cat# AAA30112)
Full Name
CD11a Antibody
Gene Names
ITGAL; CD11A; LFA-1; LFA1A
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of U-87MG cells using 3ug MAPK14 antibody. Western blot was performed from the immunoprecipitate using MAPK14 antibody at a dilition of 1:1000.)
MAPK14, Polyclonal Antibody (Cat# AAA28274)
Full Name
MAPK14 Polyclonal Antibody
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Affinity Purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-CD81 antibody. Counter stained with hematoxylin.)
TAPA1/CD81, Monoclonal Antibody (Cat# AAA30181)
Full Name
TAPA1/CD81 Antibody
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with FGFR3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FGFR3, Monoclonal Antibody (Cat# AAA30315)
Full Name
FGFR3 Antibody
Gene Names
FGFR3; ACH; CEK2; JTK4; CD333; HSFGFR3EX
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with CYP27A1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
CYP27A1, Monoclonal Antibody (Cat# AAA30522)
Full Name
CYP27A1 Antibody
Gene Names
CYP27A1; CTX; CP27; CYP27
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
EEF1A2, Polyclonal Antibody (Cat# AAA31289)
Full Name
Phospho-EEF1A2 (Ser358) Antibody
Gene Names
EEF1A2; HS1; STN; EF1A; STNL; EEF1AL; EF-1-alpha-2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Histone H4 (acetyl K16) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Histone H4, Monoclonal Antibody (Cat# AAA30539)
Full Name
Histone H4 (Acetyl K16) Antibody
Gene Names
HIST2H4B; H4/o
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Hsp70 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Hsp70, Monoclonal Antibody (Cat# AAA29972)
Full Name
Hsp70 Antibody
Gene Names
HSPA1B; HSP72; HSPA1; HSX70; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of C6 cells using anti-Arp2/ACTR2 antibody (AAA19284).Overlay histogram showing C6 cells stained with AAA19284 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Arp2/ACTR2 Antibody (AAA19284,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Arp2/ACTR2, Polyclonal Antibody (Cat# AAA19284)
Full Name
Anti-Arp2/ACTR2 Antibody
Gene Names
ACTR2; ARP2
Reactivity
Human, Mouse, Monkey, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human-breast-cancer using antibody diluted at 1:50.)
CD10, Monoclonal Antibody (Cat# AAA23996)
Full Name
CD10 (7C7) Mouse mAb
Gene Names
MME; NEP; SFE; CD10; CALLA
Reactivity
Human, Rat, Mouse
Applications
IHC-P
Purity
Affinity purified
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using S100A10 antibody at dilution of 1:100 (40x lens).)
S100A10, Polyclonal Antibody (Cat# AAA10765)
Full Name
S100A10 Polyclonal Antibody
Gene Names
S100A10; 42C; P11; p10; GP11; ANX2L; CAL1L; CLP11; Ca[1]; ANX2LG
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
ELISA
(ELISA plate was coated by 2019-nCoV NP, 100 uL/cell at 5ug/ml. The indirect ELISA analysis was performed by loading 100 uL per well of the anti-2019-nCoV N mAb (BN9) at various concentrations. The plate was incubated for 1 hours at 37 degree C, then washed 5 times. An anti hFc HRP conjugated mAb at a concentration of 1:2000, 100uL/well was used as the secondary antibody. Again, the plate was incubated for 1 hours at 37 degree C, then washed 5 times. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Signal was read on a spectrophotometer at 450 nm.)
COVID 19 Nucleocapsid (NP) (BN9) Coronavirus, Monoclonal Antibody (Cat# AAA27957)
Full Name
Human anti-2019-nCoV N mAb (BN9)
Applications
ELISA
Purity
Purity: > = 95% as determined by SDS-PAGE
Purification: Protein A
Purification: Protein A
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H4-K20 antibody (AAA10655) at dilution of 1:200 (40x lens).)
H4K20me2, Antibody (Cat# AAA10655)
Full Name
Histone H4K20me2 Polyclonal Antibody
Gene Names
HIST2H4A; H4; H4/n; H4F2; H4FN; FO108; HIST2H4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene.)
Bid, Polyclonal Antibody (Cat# AAA28769)
Full Name
Bid Antibody (BH3 Domain Specific)
Gene Names
BID; FP497
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Peroxiredoxin 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Peroxiredoxin 2, Monoclonal Antibody (Cat# AAA30240)
Full Name
Peroxiredoxin 2 Antibody
Gene Names
PRDX2; PRP; TSA; PRX2; PTX1; TPX1; NKEFB; PRXII; TDPX1; NKEF-B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
WB (Western Blot)
(Western blot of Latexin (arrow) in 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LXN gene (Lane 2))
Latexin / MUM, Polyclonal Antibody (Cat# AAA12323)
Full Name
Rabbit Polyclonal to Human Latexin / MUM
Gene Names
LXN; ECI; TCI
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry
Purity
Immunoaffinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-SOD2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Placenta)
SOD2, Polyclonal Antibody (Cat# AAA23583)
Full Name
SOD2 antibody - N-terminal region
Gene Names
SOD2; IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD
Reactivity
Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
Western Blot
Purity
Affinity Purified
Pricing
WB (Western Blot)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with OLR1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
OLR1, Polyclonal Antibody (Cat# AAA28737)
Full Name
OLR1 Antibody (Center)
Gene Names
OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)
Hsp70/Hsc70, Monoclonal Antibody (Cat# AAA17793)
Full Name
Hsp70/Hsc70 Antibody: HRP
Gene Names
HSPA2; HSP70
Reactivity
Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast
Applications
WB, IP, IHC
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
eIF4B, Polyclonal Antibody (Cat# AAA31302)
Full Name
Phospho-eIF4B (Ser504) Antibody
Gene Names
EIF4B; EIF-4B; PRO1843
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
EIF2S2, Polyclonal Antibody (Cat# AAA31456)
Full Name
Phospho-EIF2S2 (Ser2) Antibody
Gene Names
EIF2S2; EIF2; EIF2B; PPP1R67; EIF2beta; eIF-2-beta
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (83%), Rabbit (100%), Dog (83%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (83%), Rabbit (100%), Dog (83%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of LIN28B Antibody (N-term) in HL60 cell line lysates (35 ug/lane). LIN28B (arrow) was detected using the purified Pab.)
LIN28B, Polyclonal Antibody (Cat# AAA12320)
Full Name
Rabbit Polyclonal to Human LIN28B
Gene Names
LIN28B; CSDD2
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Ammonium sulfate precipitation
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of Hela cells using anti-Aquaporin 3 antibody (AAA11516).Overlay histogram showing Hela cells stained with AAA11516 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aquaporin 3 Antibody (AAA11516,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Aquaporin 3, Polyclonal Antibody (Cat# AAA11516)
Full Name
Anti-Aquaporin 3 antibody
Gene Names
AQP3; GIL; AQP-3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 6. IF analysis of SGK1 using anti- SGK1 antibody (AAA19225).SGK1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL rabbit anti- SGK1 Antibody (AAA19225) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
SGK1, Polyclonal Antibody (Cat# AAA19225)
Full Name
Anti-SGK1 Antibody
Gene Names
SGK1; SGK
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (AAA19171).SAE2/UBA2 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SAE2/UBA2 Antibody (AAA19171) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
SAE2/UBA2, Polyclonal Antibody (Cat# AAA19171)
Full Name
Anti-SAE2/UBA2 Picoband antibody
Gene Names
UBA2; ARX; SAE2; HRIHFB2115
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
TOPBP1, Polyclonal Antibody (Cat# AAA31467)
Full Name
Phospho-TOPBP1 (Ser1159) Antibody
Gene Names
TOPBP1; TOP2BP1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (80%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (80%), Xenopus (80%)
Predicted Reactivity: Pig (100%), Zebrafish (80%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (80%), Xenopus (80%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody (N-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).BHLH3 immunoreactivity is localized to nucleus significantly.)
BHLH3, Polyclonal Antibody (Cat# AAA28734)
Full Name
BHLH3 Antibody (N-term)
Gene Names
BHLHE41; DEC2; hDEC2; BHLHB3; SHARP1
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HEPA1-6 cells using anti-PI-16/PI16 antibody (AAA19330).Overlay histogram showing HEPA1-6 cells stained with AAA19330 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PI-16/PI16 Antibody (AAA19330, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PI-16/PI16, Polyclonal Antibody (Cat# AAA19330)
Full Name
Anti-PI-16/PI16 Antibody
Gene Names
PI16; PSPBP; CRISP9; MSMBBP
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
p27 Kip1, Polyclonal Antibody (Cat# AAA31387)
Full Name
Phospho-p27 Kip1 (Thr157) Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (89%), Sheep (89%), Rabbit (100%), Dog (100%), Chicken (89%)
Predicted Reactivity: Bovine (89%), Sheep (89%), Rabbit (100%), Dog (100%), Chicken (89%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse spinal cord using BRCA1 antibody at dilution of 1:100 (40x lens).)
BRCA1, Polyclonal Antibody (Cat# AAA28245)
Full Name
BRCA1 Polyclonal Antibody
Gene Names
BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells, untreated(right) and Chloroquine treated(50 ?M, 37? for 20 hours;left) using LC3B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
LC3B, Monoclonal Antibody (Cat# AAA28305)
Full Name
[KO Validated] LC3B Rabbit mAb
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells with PCK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
PCK2, Monoclonal Antibody (Cat# AAA30447)
Full Name
PCK2 Antibody
Gene Names
PCK2; PEPCK; PEPCK2; PEPCK-M
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug EIF3H antibody. Western blot was performed from the immunoprecipitate using EIF3H antibody at a dilition of 1:1000.)
EIF3H, Polyclonal Antibody (Cat# AAA28265)
Full Name
EIF3H Polyclonal Antibody
Gene Names
EIF3H; EIF3S3; eIF3-p40; eIF3-gamma
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Pricing
WB (Western Blot)
(FOLR2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human spleen followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FOLR2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
FOLR2, Polyclonal Antibody (Cat# AAA28783)
Full Name
FOLR2 Antibody (N-term)
Gene Names
FOLR2; FBP; FR-P3; FR-BETA; BETA-HFR; FBP/PL-1
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with HNF-4-alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
HNF-4-alpha, Monoclonal Antibody (Cat# AAA30162)
Full Name
HNF-4-alpha Antibody
Gene Names
HNF4A; TCF; HNF4; MODY; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of FABP7 in PC3 cells. The cell was stained with AAA11731 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
FABP7, Monoclonal Antibody (Cat# AAA11731)
Full Name
FABP7 antibody
Gene Names
FABP7; MRG; BLBP; FABPB; B-FABP
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
By protein-G affinity chromatography
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with ERK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ERK2, Monoclonal Antibody (Cat# AAA30006)
Full Name
ERK2 Antibody
Gene Names
MAPK1; ERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of 293T cells with BRD2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
BRD2, Monoclonal Antibody (Cat# AAA30523)
Full Name
BRD2 Antibody
Gene Names
BRD2; FSH; NAT; RNF3; FSRG1; RING3; D6S113E
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (AAA19144).14-3-3 zeta/delta was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-14-3-3 zeta/delta Antibody (AAA19144) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
14-3-3 zeta/delta, Polyclonal Antibody (Cat# AAA19144)
Full Name
Anti-14-3-3 zeta/delta Picoband antibody
Gene Names
YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Pricing