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898 results for "HRP Conjugated Secondary Antibody" - showing 800-850
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Cyclin E1, Polyclonal Antibody (Cat# AAA31362)
Full Name
Phospho-Cyclin E1 (Ser73) Antibody
Gene Names
CCNE1; CCNE
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA31275 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31275 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
LIF, Polyclonal Antibody (Cat# AAA31275)
Full Name
LIF Antibody
Gene Names
LIF; CDF; DIA; HILDA; MLPLI
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Bovine(88%), Sheep(88%), Dog(88%)
Predicted Reactivity: Pig(88%), Bovine(88%), Sheep(88%), Dog(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31029 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CDC25A, Polyclonal Antibody (Cat# AAA31029)
Full Name
Phospho-CDC25A (Ser75) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various tissue sample, using Phospho-PLCG1 (Tyr771) Antibody.)
PLCG1, Polyclonal Antibody (Cat# AAA31021)
Full Name
Phospho-PLCG1 (Tyr771) Antibody
Gene Names
PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30992 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
SP1, Polyclonal Antibody (Cat# AAA30992)
Full Name
Phospho-SP1 (Thr739) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31043 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
STAT1, Polyclonal Antibody (Cat# AAA31043)
Full Name
Phospho-STAT1 (Ser727) Antibody
Gene Names
STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunoflouorescence)
(AAA31056 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
LIMK2, Polyclonal Antibody (Cat# AAA31056)
Full Name
Phospho-LIMK2 (Ser283) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of FOXO4 phosphorylation expression in EGF treated HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
FOXO4, Polyclonal Antibody (Cat# AAA31061)
Full Name
Phospho-FOXO4 (Thr451) Antibody
Gene Names
FOXO4; AFX; AFX1; MLLT7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31127 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31127 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab.The nuclear counter stain is DAPI(blue).)
PRDX6, Polyclonal Antibody (Cat# AAA31127)
Full Name
PRDX6 Antibody
Gene Names
PRDX6; PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Peptide affinity purification
Pricing
IHC (Immunohistchemistry)
(AAA31049 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Caspase 3, Polyclonal Antibody (Cat# AAA31049)
Full Name
Phospho-Caspase 3 (Ser150) Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31054 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
JNK1/2/3, Polyclonal Antibody (Cat# AAA31054)
Full Name
Phospho-JNK1/2/3 (Thr183+Tyr185) Antibody
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Ezrin phosphorylation expression in Na3VO4 treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Ezrin, Polyclonal Antibody (Cat# AAA31010)
Full Name
Phospho-Ezrin (Tyr478) Antibody
Gene Names
EZR; CVL; CVIL; VIL2; HEL-S-105
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30987 at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
NPM, Polyclonal Antibody (Cat# AAA30987)
Full Name
Phospho-NPM (Thr199) Antibody
Gene Names
NPM1; B23; NPM
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term) (Cat.#AAA28756), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
EN1 (Engrailed 1), Polyclonal Antibody (Cat# AAA28756)
Full Name
EN1 (Engrailed 1) Antibody (N-term)
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Dnmt1, Polyclonal Antibody (Cat# AAA31301)
Full Name
Phospho-Dnmt1 (Ser714) Antibody
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
anti-Human IgG+HRP, Polyclonal Secondary Antibody (Cat# AAA13852)
Full Name
Goat anti-Human IgG (H+L) +HRP
Reactivity
Human
Applications
Western Blot
Pricing
anti-Mouse IgG+HRP, Secondary Antibody (Cat# AAA13853)
Full Name
anti-Mouse IgG+HRP
Reactivity
Mouse
Applications
Western Blot
Pricing
WB (Western Blot)
(Western blot: primary antibody, anti-envelope (Zika virus) rabbit polyclonal antibody (1:2000 dilution); secondary antibody, HRP-conjugated goat anti-rabbit IgG (1:4000)A, full length envelope protein of Zika virus)
Envelope (E) (Zika Virus), Antibody (Cat# AAA13695)
Full Name
Anti-Envelope (E) (Zika Virus) Polyclonal Antibody, Rabbit Serum
Applications
Western Blot
Pricing
ELISA
(ELISA plate was coated by the 2019-nCoV S1 protein, 100 uL/cell at 5ug/ml. The indirect ELISA analysis was performed by loading 100 uL per well of the anti-2019-nCoV S1 mAb (S1) at various concentrations. The plate was incubated for 1 hours at 37 degree C, then washed 5 times. An anti hFc HRP conjugated mAb at a concentration of 1:2000, 100uL/well was used as the secondary antibody. Again, the plate was incubated for 1 hours at 37 degree C, then washed 5 times. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Signal was read on a spectrophotometer at 450 nm.)
COVID 19 Spike S1 Protein Coronavirus, Monoclonal Antibody (Cat# AAA27959)
Full Name
human anti-2019-nCoV Spike S1 mAb (S1)
Applications
ELISA
Purity
Purity: ≥ 95% as determined by SDS-PAGE
Purification: Protein A
Purification: Protein A
Pricing
IHC (Immunohistochemistry)
(AAA31139 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
POLD4, Polyclonal Antibody (Cat# AAA31139)
Full Name
POLD4 Antibody
Gene Names
POLD4; p12; POLDS
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Aquaporin 4 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Aquaporin 4, Monoclonal Antibody (Cat# AAA27803)
Full Name
Anti-Aquaporin 4 Antibody
Gene Names
AQP4; MIWC; WCH4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Pricing
IHC (Immunohistochemistry)
(AAA31140 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
HOXC13, Polyclonal Antibody (Cat# AAA31140)
Full Name
HOXC13 Antibody
Gene Names
HOXC13; HOX3; ECTD9; HOX3G
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Peptide affinity purification
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31231 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H1.5, Polyclonal Antibody (Cat# AAA31231)
Full Name
Histone H1.5 Antibody
Gene Names
H1-5; H1; H1B; H1.5; H1F5; H1s-3; HIST1H1B
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(Western blot analysis of lysates from 293T cell line human brain and mouse brain tissue lysate(from left to right), using STRADA Antibody (C-term). AAA28673 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
STRADA, Polyclonal Antibody (Cat# AAA28673)
Full Name
STRADA Antibody (C-term)
Gene Names
STRADA; LYK5; PMSE; Stlk; STRAD; NY-BR-96
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Applications
Western Blot, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(AAA30934 at 1/100 staining human TB tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
I kappaB alpha, Polyclonal Antibody (Cat# AAA30934)
Full Name
Phospho-I kappaB alpha (Ser32/Ser36) Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-HDAC3 (Ser424) Antibody expression in mouse lung and mouse brain tissues lysates.The lane on the right is treated with the antigen-specific peptide.)
HDAC3, Polyclonal Antibody (Cat# AAA30951)
Full Name
Phospho-HDAC3 (Ser424) Antibody
Gene Names
HDAC3; HD3; RPD3; RPD3-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of lysate from mouse heart tissue lysate, using MYBPC3 Antibody (N-term). AAA28794 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
MYBPC3, Polyclonal Antibody (Cat# AAA28794)
Full Name
MYBPC3 Antibody (N-term)
Gene Names
MYBPC3; FHC; CMH4; CMD1MM; LVNC10; MYBP-C
Reactivity
Human, Mouse, Rat
(Predicted Reactivity: Rat)
(Predicted Reactivity: Rat)
Applications
Immunohistochemistry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(AAA31265 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31265) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
PPP2R5C, Polyclonal Antibody (Cat# AAA31265)
Full Name
PPP2R5C Antibody
Gene Names
PPP2R5C; B56G; PR61G; B56gamma
Reactivity
Human, Rat
Predicted Reactivity: Rabbit(88%)
Predicted Reactivity: Rabbit(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(AAA31059 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Smad2/3, Polyclonal Antibody (Cat# AAA31059)
Full Name
Phospho-Smad2/3 (Thr8) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide affinity columns
Pricing
IHC (Immunohistochemistry)
(AAA30948 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk1, Polyclonal Antibody (Cat# AAA30948)
Full Name
Phospho-Chk1 (Ser317) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of Chk2 phosphorylation expression in Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Chk2, Polyclonal Antibody (Cat# AAA30957)
Full Name
Phospho-Chk2 (Thr387) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(RSPO2 Antibody (C-term) (AAA28708) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
RSPO2, Polyclonal Antibody (Cat# AAA28708)
Full Name
RSPO2 Antibody (C-term)
Gene Names
RSPO2; CRISTIN2
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Westen Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(AAA31053 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
GATA4, Polyclonal Antibody (Cat# AAA31053)
Full Name
Phospho-GATA4 (Ser262) Antibody
Gene Names
GATA4; TOF; ASD2; VSD1; TACHD
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of XIAP phosphorylation expression in Anisomycin treated HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
XIAP, Polyclonal Antibody (Cat# AAA31060)
Full Name
Phospho-XIAP (Ser87) Antibody
Gene Names
XIAP; API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30995 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
BCL-2, Polyclonal Antibody (Cat# AAA30995)
Full Name
Phospho-BCL-2 (Ser70) Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(staining A549 cells cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Cox2, Polyclonal Antibody (Cat# AAA31143)
Full Name
Cox2 Antibody
Gene Names
PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
Tau, Polyclonal Antibody (Cat# AAA31002)
Full Name
Phospho-Tau (Thr231) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho- peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of p53 phosphorylation expression in HU treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
p53, Polyclonal Antibody (Cat# AAA30977)
Full Name
Phospho-p53 (Ser15) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of LoVo cell lysate, using PIBF1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
PIBF1, Polyclonal Antibody (Cat# AAA31130)
Full Name
PIBF1 Antibody
Gene Names
PIBF1; PIBF; CEP90; JBTS33; C13orf24
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-Tau (Ser214) expression in various lysates)
Tau, Polyclonal Antibody (Cat# AAA30996)
Full Name
Phospho-Tau (Ser214) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PAR4, Polyclonal Antibody (Cat# AAA31375)
Full Name
Phospho-PAR4 (Thr163) Antibody
Gene Names
PAWR; PAR4; Par-4
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U20S cells using anti-Calpastatin antibody (AAA11679).Overlay histogram showing U20S cells stained with AAA11679 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calpastatin Antibody (AAA11679,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Calpastatin, Polyclonal Antibody (Cat# AAA11679)
Full Name
Anti-Calpastatin Antibody
Gene Names
CAST; BS-17; PLACK
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U251 cells using anti-PNP antibody (AAA11680).Overlay histogram showing U251 cells stained with AAA11680 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNP Antibody (AAA11680,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PNP, Polyclonal Antibody (Cat# AAA11680)
Full Name
Anti-PNP Antibody
Gene Names
PNP; NP; PUNP; PRO1837
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 7. IF analysis of CD68 using anti- CD68 antibody CD68 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti- CD68 Antibody overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
CD68, Polyclonal Antibody (Cat# AAA11512)
Full Name
Anti-CD68 antibody
Gene Names
Cd68; Lamp4; gp110; Scard1
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence (IFC) analysis of PC3 cells using AAA14697 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Ribonuclease inhibitor 1, Monoclonal Antibody (Cat# AAA14697)
Full Name
Ribonuclease inhibitor 1 (RNH1)
Gene Names
RNH1; RAI; RNH; MGC4569; MGC18200; MGC54054
Applications
Immunofluorescence, Western Blot
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
IHC (Immunohistochemistry)
(AAA30941 at 1/100 staining Human lung cancer by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
AC-FKHR, Polyclonal Antibody (Cat# AAA30941)
Full Name
AC-FKHR(K294) Antibody
Gene Names
FOXO1; FKH1; FKHR; FOXO1A
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Cytokeratin 20 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Cytokeratin 20, Polyclonal Antibody (Cat# AAA27786)
Full Name
Anti-Cytokeratin 20 Antibody
Gene Names
KRT20; K20; CD20; CK20; CK-20; KRT21
Reactivity
Human, Mouse, Rat, Bovine, Pig
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(AAA30955 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
JAK2, Polyclonal Antibody (Cat# AAA30955)
Full Name
Phospho-JAK2 (Tyr931) Antibody
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30966 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Myc, Polyclonal Antibody (Cat# AAA30966)
Full Name
Phospho-Myc (Thr58) Antibody
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31211 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31211 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
SEPT4, Polyclonal Antibody (Cat# AAA31211)
Full Name
SEPT4 Antibody
Gene Names
SEPTIN4; H5; ARTS; MART; SEP4; CE5B3; SEPT4; PNUTL2; hucep-7; BRADEION; C17orf47; hCDCREL-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing