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8519 results for " Protein" - showing 8200-8250
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12169)
Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
EM, FC/FACS, IF, IP, RIA, RE, WB
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
RSK1 p90, Polyclonal Antibody (Cat# AAA31374)
Full Name
Phospho-RSK1 p90 (Thr573) Antibody
Gene Names
RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12173)
Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
EM, FC/FACS, IF, IP, RIA, RE, WB
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of Hela cells using anti-CD147/Emmprin antibody (AAA11678).Overlay histogram showing Hela cells stained with AAA11678 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD147/Emmprin Antibody (AAA11678,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CD147/Emmprin, Polyclonal Antibody (Cat# AAA11678)
Full Name
Anti-CD147/Emmprin Antibody
Gene Names
BSG; OK; 5F7; TCSF; CD147; EMMPRIN
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of U20S cells using anti-NFIA antibody (AAA11691).Overlay histogram showing U20S cells stained with AAA11691 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFIA Antibody (AAA11691,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NFIA, Polyclonal Antibody (Cat# AAA11691)
Full Name
Anti-NFIA Antibody
Gene Names
NFIA; CTF; NF1-A; NFI-A; NFI-L; NF-I/A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
IP (Immunoprecipitation)
(Figure 13 Immunoprecipitation Validation in HEK293 cells (Kawai et al., 2004)HEK293 cells were transiently transfected with DYKDDDDK-IRF7. Ccell lysates were immunoprecipitated with control rabbit anti-mouse immunoglobulin serum (IgG) or anti-MyD88 (Ab1 and Ab2), followed by immunoblotting with anti-DYKDDDDK.)
MYD88, Polyclonal Antibody (Cat# AAA10930)
Full Name
MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Human, Mouse
Applications
EIA, WB, IHC, IF
Purity
MYD88 Antibody is affinity chromatography purified via peptide column.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
PKC theta, Polyclonal Antibody (Cat# AAA31410)
Full Name
Phospho-PKC theta (Tyr90) Antibody
Gene Names
PRKCQ; PRKCT; nPKC-theta
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (91%), Dog (91%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (91%), Dog (91%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Figure 10 Immunofluorescence images of PUMA in the P14 rat retina (Wakabayashi et al., 2012)PUMA expression in the rat retina detected by anti-PUMA antibodies (3043). The specimens were counterstained with Hoechst 33258 to visualize nuclei (+DNA). GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; P, postnatal day.)
PUMA, Polyclonal Antibody (Cat# AAA10934)
Full Name
PUMA Antibody
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human
Applications
EIA, WB, IHC, IF
Purity
PUMA Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
(Figure 9 KD Validation in HeLa Cells (Horinaka et al., 2005)HeLa cells were transfected with DR4siRNA or LacZ control siRNA. At 24 h after transfection, the cells were treated with or without 20 μM luteolin for 24 h. Western blot analysis was carried out with anti-DR4 antibodies. DR4 expression was markedly reduced after DR4 knockdown.)
DR4, Polyclonal Antibody (Cat# AAA10950)
Full Name
DR4 Antibody
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Reactivity
Human
Applications
EIA, WB
Purity
DR4 Antibody is affinity chromatography purified via peptide column.
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA11969)
Full Name
MOUSE ANTI RAT CD11b
Gene Names
ITGAM; CD11B
Applications
FC/FACS, IF, IP
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U20S cells using anti-PPID antibody (AAA19159).Overlay histogram showing U20S cells stained with AAA19159 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPID Antibody (AAA19159,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PPID/Cyclophilin 40, Polyclonal Antibody (Cat# AAA19159)
Full Name
Anti-PPID/Cyclophilin 40 Picoband antibody
Gene Names
PPID; CYPD; CYP-40
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Abi 1, Polyclonal Antibody (Cat# AAA31442)
Full Name
Phospho-Abi 1 (Tyr213) Antibody
Gene Names
ABI1; E3B1; ABI-1; ABLBP4; NAP1BP; SSH3BP; SSH3BP1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
MCM2, Polyclonal Antibody (Cat# AAA31459)
Full Name
Phospho-MCM2 (Ser139) Antibody
Gene Names
MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (80%), Bovine (83%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (80%), Bovine (83%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of PC-3 cells using anti-ADK antibody (AAA11690).Overlay histogram showing PC-3 cells stained with AAA11690 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADK Antibody (AAA11690,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ADK, Polyclonal Antibody (Cat# AAA11690)
Full Name
Anti-ADK Antibody
Gene Names
ADK; AK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Lyn using anti-Lyn antibody (AAA19150). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 293T whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lyn antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Lyn at approximately 59KD. The expected band size for Lyn is at 59KD.)
Lyn, Polyclonal Antibody (Cat# AAA19150)
Full Name
Anti-Lyn Picoband Antibody
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of MED15 using anti-MED15 antibody (AAA19169).MED15 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED15 Antibody (AAA19169) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
MED15/Pcqap, Polyclonal Antibody (Cat# AAA19169)
Full Name
Anti-MED15/Pcqap Picoband Antibody
Gene Names
MED15; TIG1; CAG7A; CTG7A; PCQAP; TIG-1; TNRC7; ARC105
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Human mammary cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
PRC1, Polyclonal Antibody (Cat# AAA31449)
Full Name
Phospho-PRC1 (Thr470) Antibody
Gene Names
PRC1; ASE1
Reactivity
Human, Mouse
Predicted Reactivity: Pig (100%), Horse (89%), Sheep (89%)
Predicted Reactivity: Pig (100%), Horse (89%), Sheep (89%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
ATP1A1, Polyclonal Antibody (Cat# AAA31454)
Full Name
Phospho-ATP1A1 (Tyr260) Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (90%), Dog (100%), Xenopus (90%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (90%), Dog (100%), Xenopus (90%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
RBL2, Polyclonal Antibody (Cat# AAA31451)
Full Name
Phospho-RBL2 (Thr642) Antibody
Gene Names
RBL2; Rb2; P130
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (89%), Sheep (100%), Rabbit (89%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (89%), Sheep (100%), Rabbit (89%), Dog (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
TOPBP1, Polyclonal Antibody (Cat# AAA31467)
Full Name
Phospho-TOPBP1 (Ser1159) Antibody
Gene Names
TOPBP1; TOP2BP1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (80%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (80%), Xenopus (80%)
Predicted Reactivity: Pig (100%), Zebrafish (80%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (80%), Xenopus (80%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published clone specific image: Flow cytometric analysis of AM from NO2-exposed and control rats. Rats were exposed to NO2 for the indicated times and BAL cells were stained with antibodies to ED7, ED9, RM-4, and OX-6. To overcome autofluorescence signals, primary antibodies were detected using a biotin-PE/streptavidin-anti-streptavidin enhancing system and labeling of AM was analyzed by flow cytometry following gating by help of forward and sideward scatter properties. Shown are representative results of at least six animals per group.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)
CD172a, Monoclonal Antibody (Cat# AAA12001)
Full Name
MOUSE ANTI RAT CD172a
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
FC/FACS, IP, WB
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of Integrin beta 4/ITGB4 using anti-Integrin beta 4/ITGB4 antibody (AAA19269).Integrin beta 4/ITGB4 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Integrin beta 4/ITGB4 Antibody (AAA19269) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
mGluR1/GRM1, Polyclonal Antibody (Cat# AAA19269)
Full Name
Anti-mGluR1/GRM1 Antibody
Gene Names
GRM1; MGLU1; GPRC1A; MGLUR1; SCAR13; PPP1R85
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of PC-3 cells using anti-APP antibody (AAA11597).Overlay histogram showing PC-3 cells stained with AAA11597 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APP Antibody (AAA11597,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
beta Amyloid, Polyclonal Antibody (Cat# AAA11597)
Full Name
Anti-beta Amyloid Antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HEPA1-6 cells using anti-PAR4/Pawr antibody (AAA19278).Overlay histogram showing HEPA1-6 cells stained with AAA19278 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAR4/Pawr Antibody (AAA19278, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PAR4/Pawr, Polyclonal Antibody (Cat# AAA19278)
Full Name
Anti-PAR4/Pawr Antibody
Gene Names
Pawr; PAR4; Par-4; 2310001G03Rik
Reactivity
Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of CACO-2 cells using anti-Galectin 2/LGALS2 antibody (AAA19287).Overlay histogram showing CACO-2 cells stained with AAA19287 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Galectin 2/LGALS2 Antibody (AAA19287, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Galectin 2/LGALS2, Polyclonal Antibody (Cat# AAA19287)
Full Name
Anti-Galectin 2/LGALS2 Antibody
Gene Names
LGALS2; HL14
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HEPA1-6 cells using anti-Rad9/Rad9a antibody (AAA19288).Overlay histogram showing HEPA1-6 cells stained with AAA19288 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rad9/Rad9a Antibody (AAA19288,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rad9/Rad9a, Polyclonal Antibody (Cat# AAA19288)
Full Name
Anti-Rad9/Rad9a Antibody
Gene Names
Rad9a; Rad9
Reactivity
Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of A549 cells using anti-AGO3 antibody (AAA19290).Overlay histogram showing A549 cells stained with AAA19290 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AGO3 Antibody (AAA19290, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
AGO3, Polyclonal Antibody (Cat# AAA19290)
Full Name
Anti-AGO3 Antibody
Gene Names
AGO3; EIF2C3
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HELA cells using anti-PDE6 alpha/PDE6A antibody (AAA19318).Overlay histogram showing HELA cells stained with AAA19318 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDE6 alpha/PDE6A Antibody (AAA19318, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PDE6 alpha/PDE6A, Polyclonal Antibody (Cat# AAA19318)
Full Name
Anti-PDE6 alpha/PDE6A Antibody
Gene Names
PDE6A; PDEA; RP43; CGPR-A; PDE6A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of Hela cells using anti-Caspase-9/CASP9 antibody (AAA19215).Overlay histogram showing Hela cells stained with AAA19215 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-9/CASP9 Antibody (AAA19215, 1 μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Caspase-9/CASP9, Polyclonal Antibody (Cat# AAA19215)
Full Name
Anti-Caspase-9/CASP9 Antibody
Gene Names
CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
Reactivity
Human
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U251 cells using anti-Serum Response Factor/SRF antibody (AAA19221).Overlay histogram showing U251 cells stained with AAA19221 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Serum Response Factor/SRF Antibody (AAA19221, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Serum Response Factor/SRF, Polyclonal Antibody (Cat# AAA19221)
Full Name
Anti-Serum Response Factor/SRF Antibody
Gene Names
SRF; MCM1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-Caspase-7/CASP7 antibody (AAA19232).Overlay histogram showing MCF-7 cells stained with AAA19232 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-7/CASP7 Antibody (AAA19232, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Caspase-7/CASP7, Polyclonal Antibody (Cat# AAA19232)
Full Name
Anti-Caspase-7/CASP7 Antibody
Gene Names
CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
Reactivity
Human, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of RH35 cells using anti-Prohibitin/PHB antibody (AAA19234).Overlay histogram showing RH35 cells stained with AAA19234 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Prohibitin/PHB Antibody (AAA19234, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Prohibitin/PHB, Polyclonal Antibody (Cat# AAA19234)
Full Name
Anti-Prohibitin/PHB Antibody
Gene Names
PHB; PHB1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of U251 cells using anti-GABARAP antibody (AAA19250).Overlay histogram showing U251 cells stained with AAA19250 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GABARAP Antibody (AAA19250, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GABARAP, Polyclonal Antibody (Cat# AAA19250)
Full Name
Anti-GABARAP Antibody
Gene Names
GABARAP; MM46; ATG8A; GABARAP-a
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of U937 cells using anti-GNG7 antibody (AAA19335).Overlay histogram showing U937 cells stained with AAA19335 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GNG7 Antibody (AAA19335, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GNG7, Polyclonal Antibody (Cat# AAA19335)
Full Name
Anti-GNG7 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of MCF-7 cells using anti-DDX1 antibody (AAA19378).Overlay histogram showing MCF-7 cells stained with AAA19378 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- DDX1 Antibody (AAA19378, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DDX1, Monoclonal Antibody (Cat# AAA19378)
Full Name
Anti-DDX1 Antibody (monoclonal, 3I10)
Gene Names
DDX1; DBP-RB; UKVH5d
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of MCF-7 cells using anti- PCK2 antibody (AAA19384).Overlay histogram showing MCF-7 cells stained with AAA19384 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PCK2 Antibody (AAA19384, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PCK2, Monoclonal Antibody (Cat# AAA19384)
Full Name
Anti-PCK2 Antibody (monoclonal, 3F7)
Gene Names
PCK2; PEPCK; PEPCK2; PEPCK-M
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of SiHa cells using anti- Aldolase/ALDOA antibody (AAA19385).Overlay histogram showing SiHa cells stained with AAA19385 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Aldolase/ALDOA Antibody (AAA19385, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Aldolase/ALDOA, Monoclonal Antibody (Cat# AAA19385)
Full Name
Anti-Aldolase/ALDOA Antibody (monoclonal, 6H8)
Gene Names
ALDOA; ALDA; GSD12
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of A549 cells using anti-Ch TOG/CKAP5 antibody (AAA19386).Overlay histogram showing A549 cells stained with AAA19386 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Ch TOG/CKAP5 Antibody (AAA19386, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ch TOG/CKAP5, Monoclonal Antibody (Cat# AAA19386)
Full Name
Anti-ch TOG/CKAP5 Antibody (monoclonal, 3C13)
Gene Names
CKAP5; TOG; MSPS; TOGp; CHTOG; ch-TOG
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(PCNA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
PCNA, Polyclonal Antibody (Cat# AAA28705)
Full Name
PCNA Antibody (C-term)
Gene Names
PCNA; ATLD2
Reactivity
Human (Predicted Reactivity: Bovine, Hamster, Monkey, Rat)
Applications
EIA, IHC, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(Analysis of Protein Array containing >19, 000 full-length human proteins using EpCAM Mouse Monoclonal Antibody (EGP40/1372) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Ep-CAM/CD326, Monoclonal Antibody (Cat# AAA23890)
Full Name
Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker)
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human. Others not known.
Applications
FC/FACS, IF, WB, IHC
Pricing
WB (Western Blot)
(Western BlotSample: Recombinant GAL9, Human;Antibody: Rabbit Anti-Human GAL9 Ab)
Galectin 9 (GAL9), Active Protein (Cat# AAA21103)
Full Name
Active Galectin 9 (GAL9)
Gene Names
LGALS9; HUAT; LGALS9A
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>90%
Pricing
IHC (Immunohistochemistry)
(AAA31085 at 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
C-Kit, Polyclonal Antibody (Cat# AAA31085)
Full Name
C-Kit Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117; MASTC
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
Application Data
(Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
CD11b, Monoclonal Antibody (Cat# AAA12146)
Full Name
MOUSE ANTI RAT CD11b:FITC
Gene Names
ITGAM; CD11B
Applications
FC/FACS
Pricing
WB (Western Blot)
(Western blot analysis of PAK1 expression in Etoposide treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
PAK1, Polyclonal Antibody (Cat# AAA31096)
Full Name
PAK1 Antibody
Gene Names
PAK1; PAKalpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12170)
Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
EM, FC/FACS, IF, IP, RIA, RE, WB
Pricing
Application Data
(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
F4/80, Monoclonal Antibody (Cat# AAA12165)
Full Name
RAT ANTI MOUSE F4/80:Low Endotoxin
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
FC/FACS, FN, IP, RIA, RE, WB
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA27026 (red line) at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
CD44, Monoclonal Antibody (Cat# AAA27026)
Full Name
CD44 Monoclonal Antibody
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Human
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
>95%, Protein G Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence Analysis of PFA fixed U87 cells labeling VCL-Monospecific Mouse Monoclonal Antibody (VCL/3617) followed by Goat anti-mouse IgG-CF488 (Green).)
Vinculin, Monoclonal Antibody (Cat# AAA23953)
Full Name
Vinculin (Marker of Age-related Macular Degeneration)
Gene Names
VCL; MV; MVCL; CMD1W; CMH15; HEL114
Reactivity
Human, Mouse, Rat, Cow, Pig, Rabbit, Frog, Fish, Bird
Applications
FC/FACS, IF, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Application Data
(Detect RNase residue by agarose gel electrophores)
Proteinase K-lyophilization, Protein (Cat# AAA28067)
Full Name
Proteinase K-lyophilization
Purity
>=95% by SDS-PAGE
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
JAK3, Polyclonal Antibody (Cat# AAA31404)
Full Name
Phospho-JAK3 (Tyr904) Antibody
Gene Names
JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Sheep (100%), Dog (100%)
Predicted Reactivity: Bovine (100%), Sheep (100%), Dog (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing