Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
1056 results for "Conjugated Primary Antibody" - showing 750-800
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse Beta-glucan Receptor: FITC)
DECTIN-1, Monoclonal Antibody (Cat# AAA12126)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5Y cells with PrP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Prion Protein (PrP), Monoclonal Antibody (Cat# AAA30117)
Full Name
Prion Protein (PrP) Antibody
Gene Names
PRNP; CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31201 at 1/100 staining Human lung cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
PICALM, Polyclonal Antibody (Cat# AAA31201)
Full Name
PICALM Antibody
Gene Names
PICALM; LAP; CALM; CLTH
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry-Paraffin)
(AAA31252 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
KDM5C/Jarid1C/SMCX, Polyclonal Antibody (Cat# AAA31252)
Full Name
KDM5C/Jarid1C/SMCX Antibody
Gene Names
KDM5C; MRXJ; SMCX; MRX13; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31253 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31253 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Connexin 40/GJA5, Polyclonal Antibody (Cat# AAA31253)
Full Name
Connexin 40/GJA5 Antibody
Gene Names
GJA5; CX40; ATFB11
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31189 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31189 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Lgi1, Polyclonal Antibody (Cat# AAA31189)
Full Name
Lgi1 Antibody
Gene Names
LGI1; EPT; ETL1; ADLTE; ADPAEF; ADPEAF; IB1099; EPITEMPIN
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31195 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31195 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
P2X3, Polyclonal Antibody (Cat# AAA31195)
Full Name
P2X3 Antibody
Gene Names
P2RX3; P2X3
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31116 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31116 1:200) and mouse antibeta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
PLCB2, Polyclonal Antibody (Cat# AAA31116)
Full Name
PLCB2 Antibody
Gene Names
PLCB2; PLC-beta-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific)
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
ENSA, Polyclonal Antibody (Cat# AAA31358)
Full Name
Phospho-ENSA (Ser67/Ser62) Antibody
Gene Names
ENSA; ARPP-19e
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31079 at 1/200 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
MCL1, Polyclonal Antibody (Cat# AAA31079)
Full Name
MCL1 Antibody
Gene Names
MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of Akt phosphorylation expression in B2C Cell line lysates, The lane on the right is treated with the antigen-specific peptide.)
Akt, Polyclonal Antibody (Cat# AAA31035)
Full Name
Phospho-Akt (Ser124) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse Beta-glucan Receptor: FITC)
DECTIN-1, Monoclonal Antibody (Cat# AAA12134)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
WB (Western Blot)
(Western blot analysis of ZO-1 using various lysatesLanes 1 - 2: Merged signal (red and green). Green - AAA31075 observed at 195 kDa. Red - loading control, , observed at 55 kDa. Blots were developed with Goat Anti- Rabbit IgG(H+L) FITC–conjugated and Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated secondary antibodies)
ZO 1, Polyclonal Antibody (Cat# AAA31075)
Full Name
ZO 1 Antibody
Gene Names
TJP1; ZO-1
Reactivity
Human, Mouse, Rat, Pig, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil.)
CCR2, Monoclonal Antibody (Cat# AAA30174)
Full Name
CCR2 Antibody
Gene Names
CCR2; CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
Affinity-chromatography
Pricing
IHC (Immunohistchemistry-Paraffin)
(AAA31218 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Siglec 7, Polyclonal Antibody (Cat# AAA31218)
Full Name
Siglec 7 Antibody
Gene Names
SIGLEC7; p75; QA79; AIRM1; CD328; CDw328; D-siglec; SIGLEC-7; SIGLECP2; SIGLEC19P; p75/AIRM1
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(AAA31119 at 1/100 staining mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.)
GPRC5A, Polyclonal Antibody (Cat# AAA31119)
Full Name
GPRC5A Antibody
Gene Names
GPRC5A; RAI3; TIG1; RAIG1; GPCR5A; PEIG-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31236 at 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
OVOL2, Polyclonal Antibody (Cat# AAA31236)
Full Name
OVOL2 Antibody
Gene Names
OVOL2; CHED; CHED1; CHED2; PPCD1; ZNF339; EUROIMAGE566589
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31178 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31178) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
CENPB, Polyclonal Antibody (Cat# AAA31178)
Full Name
CENPB Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%)
Predicted Reactivity: Pig(100%), Bovine(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31193 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31193 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
CHD4, Polyclonal Antibody (Cat# AAA31193)
Full Name
CHD4 Antibody
Gene Names
CHD4; CHD-4; Mi-2b; SIHIWES; Mi2-BETA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31215 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31215 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PDE11A, Polyclonal Antibody (Cat# AAA31215)
Full Name
PDE11A Antibody
Gene Names
PDE11A; PPNAD2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Predicted Reactivity: Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31225 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31225) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
KCNK9, Polyclonal Antibody (Cat# AAA31225)
Full Name
KCNK9 Antibody
Gene Names
KCNK9; KT3.2; TASK3; K2p9.1; TASK-3
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Chicken(83%), Xenopus(83%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Chicken(83%), Xenopus(83%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31141 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31141 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
MUC5B, Polyclonal Antibody (Cat# AAA31141)
Full Name
MUC5B Antibody
Gene Names
MUC5B; MG1; MUC5; MUC9; MUC-5B
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
MAP2, Polyclonal Antibody (Cat# AAA31338)
Full Name
MAP2 Antibody
Gene Names
MAP2; MAP2A; MAP2B; MAP2C
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-KIT (Tyr703) expression in various lysates)
KIT, Polyclonal Antibody (Cat# AAA31006)
Full Name
Phospho-KIT (Tyr703) Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117; MASTC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IRAK1, Polyclonal Antibody (Cat# AAA31368)
Full Name
Phospho-IRAK1 (Thr209) Antibody
Gene Names
IRAK1; IRAK; pelle
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse (100%), Dog (82%)
Predicted Reactivity: Horse (100%), Dog (82%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12081)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
Western Blot
Pricing
IHC (Immunohistochemistry)
(AAA31106 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
GPR141, Polyclonal Antibody (Cat# AAA31106)
Full Name
GPR141 Antibody
Gene Names
GPR141; PGR13
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31258 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
L3MBTL1, Polyclonal Antibody (Cat# AAA31258)
Full Name
L3MBTL1 Antibody
Gene Names
L3MBTL1; L3MBTL; ZC2HC3; H-L(3)MBT; dJ138B7.3
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Predicted Reactivity: Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry)
(AAA31038 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IRS-1, Polyclonal Antibody (Cat# AAA31038)
Full Name
Phospho-IRS-1 (Ser639) Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31245 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Nesprin 1, Polyclonal Antibody (Cat# AAA31245)
Full Name
Nesprin 1 Antibody
Gene Names
SYNE1; 8B; AMCM; CPG2; ARCA1; EDMD4; KASH1; MYNE1; Nesp1; SCAR8; C6orf98; dJ45H2.2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse(88%), Sheep(100%), Dog(88%)
Predicted Reactivity: Horse(88%), Sheep(100%), Dog(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells, using Cytochrome P450 39A1 Antibody. The lane on the left was treated with blocking peptide.)
Cytochrome P450 39A1, Polyclonal Antibody (Cat# AAA31117)
Full Name
Cytochrome P450 39A1 Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IF (Immunofluorescence)
(AAA31272 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31272 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
DERL2, Polyclonal Antibody (Cat# AAA31272)
Full Name
DERL2 Antibody
Gene Names
DERL2; FLANa; F-LANa; CGI-101; F-LAN-1; derlin-2; DERtrin-2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31246 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31246) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
TROY, Polyclonal Antibody (Cat# AAA31246)
Full Name
TROY Antibody
Gene Names
TNFRSF19; TAJ; TROY; TRADE; TAJ-alpha
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse(88%), Sheep(86%)
Predicted Reactivity: Horse(88%), Sheep(86%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31254 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Hhip, Polyclonal Antibody (Cat# AAA31254)
Full Name
Hhip Antibody
Gene Names
HHIP; HIP
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry
Purity
Peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Ephrin B1/B2/B3, Polyclonal Antibody (Cat# AAA31292)
Full Name
Phospho-Ephrin B1/B2/B3 (Tyr324) Antibody
Gene Names
EFNB1; CFND; CFNS; EFL3; EPLG2; Elk-L; LERK2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA31213 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31213 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Exportin-5, Polyclonal Antibody (Cat# AAA31213)
Full Name
Exportin-5 Antibody
Gene Names
XPO5; exp5
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(100%)
Predicted Reactivity: Pig(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31179 at 1/100 staining human breast carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
CD36, Polyclonal Antibody (Cat# AAA31179)
Full Name
CD36 Antibody
Gene Names
CD36; FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA31230 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31230 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
ACTR1A, Polyclonal Antibody (Cat# AAA31230)
Full Name
ACTR1A Antibody
Gene Names
ACTR1A; ARP1; Arp1A; CTRN1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Ntera-2 cells stained with CSB-MA018403A0m (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
OCT4, Monoclonal Antibody (Cat# AAA27015)
Full Name
OCT4 Monoclonal Antibody
Gene Names
POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
>95%
Protein G Purified
Protein G Purified
Pricing
IF (Immunofluorescence)
(AAA31177 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31177 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Proteasome 20S LMP7, Polyclonal Antibody (Cat# AAA31177)
Full Name
Proteasome 20S LMP7 Antibody
Gene Names
PSMB8; JMP; ALDD; LMP7; NKJO; D6S216; PRAAS1; PSMB5i; RING10; D6S216E
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse(86%), Rabbit(100%), Dog(88%)
Predicted Reactivity: Horse(86%), Rabbit(100%), Dog(88%)
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC-P (Immunohistochemistry-Paraffin)
(At 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediatedantigen retrieval step in citrate buffer was performed. Thetissue was then blocked and incubated with the antibody for1.5 hours at 22°C. An HRP conjugated goat anti-rabbitantibody was used as the secondary antibody.)
c-Kit, Polyclonal Antibody (Cat# AAA31007)
Full Name
Phospho-c-Kit (Tyr721) Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117; MASTC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
Application Data
(AAA31020 staining HepG2 cells(30min of 4uM Forskolin treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31020) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue))
VEGFR1, Polyclonal Antibody (Cat# AAA31020)
Full Name
Phospho-VEGFR1 (Tyr1213) Antibody
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31051 at 1/200 staining Human bladder cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
SYK, Polyclonal Antibody (Cat# AAA31051)
Full Name
Phospho-SYK (Tyr348) Antibody
Gene Names
SYK; p72-Syk
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(HHLA2 Antibody (N-term) western blot analysis in MDA-MB453,CEM,HL-60,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HHLA2 antibody detected the HHLA2 protein (arrow).)
HHLA2, Polyclonal Antibody (Cat# AAA28722)
Full Name
HHLA2 Antibody (N-term)
Gene Names
HHLA2; B7H7; B7-H7
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using AAA28677 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
ATG7, Polyclonal Antibody (Cat# AAA28677)
Full Name
ATG7 Antibody (C-term)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human, Mouse
Predicted reactivity: Chicken, Rat
Predicted reactivity: Chicken, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western blot analysis of p53 phosphorylation expression in Etoposide treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
p53, Polyclonal Antibody (Cat# AAA30976)
Full Name
Phospho-p53 (Ser392) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(TGFBR2 Antibody western blot of mouse lung tissue lysates (35 ug/lane). The TGFBR2 antibody detected the TGFBR2 protein (arrow).)
TGFBR2, Polyclonal Antibody (Cat# AAA21269)
Full Name
Anti-TGFBR2 Antibody (aa13-40) IHC-plus
Gene Names
TGFBR2; AAT3; FAA3; LDS2; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TGFR-2; TGFbeta-RII
Reactivity
Mouse, Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Protein A purified
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse Beta-glucan Receptor: FITC)
DECTIN-1, Monoclonal Antibody (Cat# AAA12137)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H2B, Polyclonal Antibody (Cat# AAA31352)
Full Name
Acetyl-Histone H2B (Lys20) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12095)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:FITC
Applications
Flow Cytometry
Pricing