Search results for "Cell Culture"

1-Desamino 8D Arginine Vasopressin (DDAVP), ELISA Kit (Cat# AAA22844)

Full Name

General 1-Desamino 8D Arginine Vasopressin (DDAVP) ELISA Kit

Reactivity

General

Pricing

Myeloid/lymphoid or mixed lineage leukemia 1, ELISA Kit (Cat# AAA17243)

Full Name

Human Myeloid/lymphoid or mixed lineage leukemia 1 ELISA Kit

Gene Names

MLLT1; ENL; LTG19; YEATS1

Reactivity

Human

Pricing

Folic Acid (FA), ELISA Kit (Cat# AAA20065)

Full Name

General Folic Acid (FA) ELISA Kit

Reactivity

General

Pricing

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Application Data (Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD83:RPE)

CD83, Monoclonal Antibody (Cat# AAA12220)

Full Name

RAT ANTI MOUSE CD83:FITC

Applications

Flow Cytometry

Pricing

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CD4, Monoclonal Antibody (Cat# AAA11926)

Full Name

MOUSE ANTI HUMAN CD4

Gene Names

CD4; CD4mut

Applications

Immunohistochemistry, Flow Cytometry

Pricing

Protein Disulfide-Isomerase A3 (PDIA3), ELISA Kit (Cat# AAA31491)

Full Name

Human Protein Disulfide-Isomerase A3 (PDIA3) ELISA Kit

Gene Names

PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n

Reactivity

Human

Pricing

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Application Data (Published customer image: Mouse anti Human CD49d antibody, clone HP2/1 used for binding efficiency determinationImage caption:Binding efficiencies (BE) of different a4beta7 molecules composed of distinct a4 mutants to monoclonal antibodies against a4, beta7 or the a4beta7 heterodimer. Binding efficiency is determined by the ratio between the mean fluorescence of antibody binding to each a4 molecule and of the binding in a mock-transfected cell culture (see Materials and Methods for details). Dark gray bars represent binding to the human (wild type) a4 clone, whereas light gray bars are those of binding to the different a4 mutants (as shown in the x-axis). a4 mutants which included substitutions at codon 201 are boxed. A, binding of anti-a4 2b4 antibody. B, binding of anti-a4 HP2/1 antibody. C, BE of different anti-a4 and beta7 antibodies to the human a4 and the quintuple a4 mutant (5 aa mut). Bars represent the range of standard errors deduced from triplicate experiments. p-values of Student's t tests are shown above each comparison. NS, non-significant (> 0.05).From:Darc M, Hait SH, Soares EA, Cicala C, Seuanez HN, et al. (2011) Polymorphisms in the a4 Integrin of Neotropical Primates: Insights for Binding of Natural Ligands and HIV-1 gp120 to the Human a4beta7. PLoS ONE 6(9): e24461.)

Application Data (Published customer image: Mouse anti Human CD49d antibody, clone HP2/1 used for western blotting and immunoprecipitationImage caption:Tetraspanins CD81, CD82 and CD151 are associated with a4beta1 throughout erythroid maturation and with beta3 in proerythroblasts and basophilic erythroblasts. A. CD81, CD82 and CD151 precipitates from Mn2+-activated proerythroblasts (ProEB, day 5), basophilic (BasoEB, day 8) and polychromatic (PolyEB, day 12) erythroblasts were successively probed with anti-a4, anti-beta1 and anti-beta3 antibodies; tetraspanin controls from each time point are also illustrated. All tetraspanins co-precipitated a4 and beta1 from erythroblasts B. Tetraspanin precipitates from day 6 proerythroblasts (ProEB) solubilised in the presence of EDTA or different cations, and from Mn2+-activated basophilic erythroblasts (BasoEB, day 8) were probed with a mix of antibodies to a5, beta1, beta2 and beta3 integrins while the control samples were probed with the relevant tetraspanin antibodies. For clarity, integrin controls are illustrated for the EDTA blot but were present on all blots. beta1 and beta3 integrins were precipitated well only in the presence of Mn2+. C. CD81 and CD82 precipitates from day 5 proerythroblasts were successively probed with different anti-integrin subunit antibodies and demonstrate co-precipitation of beta1 and beta3 but not a5 or beta2 integrins. D. CD81 (454720) and CD82 (53H5) precipitates from day 6 proerythroblasts (ProEB) and HEL cells (HEL) solubilised in the presence of EDTA, Ca2++Mg2+ or Mn2+ probed with anti-CD82 and anti-CD81 antibodies. Each tetraspanin co-precipitates the other most strongly in the presence of Mn2+ from proerythroblasts while any cation permits co-precipitation in HEL cells. Integrins were analysed on 7.5% gels, tetraspanins on 12% gels; non-reducing conditions. Unless stated, the following clones were used: CD53, MEM-53; CD63, MEM-259; CD81, 454720; CD82, TS82b; CD151, IIG5a; a4, HP2/1; a5, IIA1; aL, TS1/22; aIIb, PAB-1. All day 5 and 6 cultures comprised 90 -95% proerythroblasts; day 8 culture comprised 5% proerythroblasts, 81% basophilic erythroblasts and 14% polychromatic erythroblasts; day 12 culture comprised 41% polychromatic erythroblasts, 15% orthochromatic erythroblasts and 41% reticulocytes. In the day 5 and 6 cultures 15 -34% of cells were GPA+ and 28 -35% of cells were aIIb+. Day 8 and day 12 cultures had 77% and 97% GPA+ cells, respectively, and 9% and 0% aIIb+ cells, respectively.From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, et al. (2013) Tetraspanins CD81 and CD82 Facilitate a4beta1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1. PLoS ONE 8(5): e62654.)

CD49d, Monoclonal Antibody (Cat# AAA12003)

Full Name

MOUSE ANTI HUMAN CD49d

Gene Names

ITGA4; IA4; CD49D

Applications

Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation

Pricing

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CD11b, Monoclonal Antibody (Cat# AAA12181)

Full Name

RAT ANTI MOUSE CD11b

Gene Names

Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik

Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation

Pricing

Vitamin D Receptor, ELISA Kit (Cat# AAA16660)

Full Name

Mouse Vitamin D Receptor ELISA Kit

Reactivity

Mouse

Pricing

15-deoxy-Prostaglandin J2, ELISA Kit (Cat# AAA16799)

Full Name

Rat 15-deoxy-Prostaglandin J2 ELISA Kit

Reactivity

Rat

Pricing

Immunoglobulin A (IgA), ELISA Kit (Cat# AAA12895)

Full Name

Porcine Immunoglobulin A (IgA) ELISA Kit

Reactivity

Porcine

Pricing

Protein Carbonyl, PC, ELISA Kit (Cat# AAA11148)

Full Name

Human Protein Carbonyl, PC ELISA Kit

Reactivity

Human

Pricing

angiopoietin 1, ELISA Kit (Cat# AAA18050)

Full Name

Human Angiopoietin-1, ANGPT1 ELISA Kit

Gene Names

ANGPT1; AGP1; AGPT; ANG1

Reactivity

Human

Pricing

Ovalbumin specific Immunoglobulin G, ELISA Kit (Cat# AAA17043)

Full Name

Porcine Ovalbumin specific Immunoglobulin G ELISA Kit

Reactivity

Porcine

Pricing

Interleukin 4 (IL-4), ELISA Kit (Cat# AAA12904)

Full Name

Guinea pig Interleukin 4 (IL-4) ELISA Kit

Gene Names

IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1

Reactivity

Guinea Pig

Pricing

Undercarboxylated Osteocalcin (ucOC), ELISA Kit (Cat# AAA15847)

Full Name

Mouse Undercarboxylated Osteocalcin, ucOC ELISA Kit

Reactivity

Mouse

Pricing

25 Hydroxy Vitamin D3, ELISA Kit (Cat# AAA17051)

Full Name

Porcine 25 Hydroxy Vitamin D3 ELISA Kit

Reactivity

Porcine

Pricing

TNF alpha, ELISA Kit (Cat# AAA17896)

Full Name

Human TNF alpha ELISA Kit

Gene Names

TNF; DIF; TNFA; TNFSF2; TNF-alpha

Reactivity

Human

Applications

SE

Pricing

Procollagen Type III N-Terminal Propeptide, ELISA Kit (Cat# AAA17006)

Full Name

Porcine Procollagen Type III N-Terminal Propeptide ELISA Kit

Reactivity

Porcine

Pricing

Interleukin 12 p40, ELISA Kit (Cat# AAA17245)

Full Name

Canine Interleukin 12 p40 ELISA Kit

Gene Names

Il12b; p40; Il-12b; Il12p40; Il-12p40

Reactivity

Canine

Pricing

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:RPE)

CD33, Monoclonal Antibody (Cat# AAA12027)

Full Name

MOUSE ANTI HUMAN CD33:RPE

Gene Names

CD33; p67; SIGLEC3; SIGLEC-3

Applications

Flow Cytometry

Pricing

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Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . Medium power)

CD13, Monoclonal Antibody (Cat# AAA12114)

Full Name

RAT ANTI MOUSE CD13:FITC

Gene Names

Anpep; Apn; AP-M; AP-N; Cd13; P150

Applications

Flow Cytometry

Pricing

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CD204, Monoclonal Antibody (Cat# AAA12075)

Full Name

RAT ANTI MOUSE CD204:Low Endotoxin

Gene Names

Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1

Applications

Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot

Pricing

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Application Data (Published clone specific image: Flow cytometric analysis of AM from NO2-exposed and control rats. Rats were exposed to NO2 for the indicated times and BAL cells were stained with antibodies to ED7, ED9, RM-4, and OX-6. To overcome autofluorescence signals, primary antibodies were detected using a biotin-PE/streptavidin-anti-streptavidin enhancing system and labeling of AM was analyzed by flow cytometry following gating by help of forward and sideward scatter properties. Shown are representative results of at least six animals per group.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)

CD172a, Monoclonal Antibody (Cat# AAA12001)

Full Name

MOUSE ANTI RAT CD172a

Gene Names

Sirpa; Bit; Ptpns1; SHPS-1

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot

Pricing

Blood Urea Nitrogen (BUN), ELISA Kit (Cat# AAA22765)

Full Name

Mouse Blood Urea Nitrogen (BUN) ELISA Kit

Reactivity

Mouse

Pricing

Rat Glucagon Like Peptide 1 ELISA Kit

Reactivity

Rat

Pricing

Interferon gamma, ELISA Kit (Cat# AAA16295)

Full Name

Human Interferon gamma ELISA Kit

Gene Names

IFNG; IFG; IFI

Reactivity

Human

Pricing

Complement fragment 4a, ELISA Kit (Cat# AAA17176)

Full Name

Monkey Complement fragment 4a ELISA Kit

Gene Names

C4A; C4; RG; C4S; CO4; C4A2; C4A3; C4A4; C4A6; C4AD; CPAMD2

Reactivity

Monkey

Pricing

cyclooxygenase-2, COX-2, ELISA Kit (Cat# AAA11259)

Full Name

Human cyclooxygenase-2, COX-2 ELISA Kit

Gene Names

PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS

Reactivity

Human

Pricing

apoprotein B100, apo-B100, ELISA Kit (Cat# AAA11089)

Full Name

Mouse apoprotein B100, apo-B100 ELISA Kit

Gene Names

APOB; FLDB; LDLCQ4; apoB-48; apoB-100

Reactivity

Mouse

Pricing

Cyclooxygenase 2, ELISA Kit (Cat# AAA16277)

Full Name

Rat Cyclooxygenase 2 ELISA Kit

Gene Names

PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS

Reactivity

Rat

Pricing

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Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . Low power)

CD86, Monoclonal Antibody (Cat# AAA12222)

Full Name

MOUSE ANTI RAT CD86

Gene Names

CD86; B70; B7-2; B7.2; LAB72; CD28LG2

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Pricing

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Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Medium power)