Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
939 results for " B Cells" - showing 750-800
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse Beta-glucan Receptor: FITC)
DECTIN-1, Monoclonal Antibody (Cat# AAA12134)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
Application Data
(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody , red an A and Mouse anti Rat CD8 MCA48), green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA11983)
Full Name
MOUSE ANTI RAT CD8 ALPHA
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12093)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA
Reactivity
Dog, Dolphin, Ferret, Fin Whale, Goat, Horse, Human, Mink, Rabbit, Pig, Sheep.
Based on sequence similarity, is expected to react with: Mustelid
N.B. Antibody reactivity and working conditions may vary between species.
Based on sequence similarity, is expected to react with: Mustelid
N.B. Antibody reactivity and working conditions may vary between species.
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12194)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12198)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
Flow Cytometry
Pricing
Phospholipase B-Like 2 (PLBL2), Recombinant Protein (Cat# AAA14457)
Full Name
Purified Phospholipase B-Like 2 Protein (PLBL2) CHO-S
Applications
Western Blot
Pricing
CD105, Monoclonal Antibody (Cat# AAA14292)
Full Name
CD105 antibody (biotin)
Reactivity
To be determined by end-user.
Applications
Flow Cytometry, Immunohistochemistry
Purity
>95% pure
CD105 antibody (biotin) was purified by Protein A chromatography.
CD105 antibody (biotin) was purified by Protein A chromatography.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27685)
Full Name
VPS35 Antibody, Clone 8A3: ATTO 594
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IF (Immunofluorescence)
(AAA31178 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31178) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
CENPB, Polyclonal Antibody (Cat# AAA31178)
Full Name
CENPB Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%)
Predicted Reactivity: Pig(100%), Bovine(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
Application Data
(Published customer image: Dynamics of Th17 cells. D -F) Photographs of double immunolabelled sections showing a representative CD4+ROR?+ cell (arrow) observed around blood vessels (BV). Bar scale = 30 um.Almolda B, Costa M, Montoya M, Gonz¡lez B, Castellano B (2011) Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat. PLoS ONE 6(11): e27473.)
CD4, Monoclonal Antibody (Cat# AAA11994)
Full Name
MOUSE ANTI RAT CD4 (DOMAIN 1)
Gene Names
Cd4; p55; W3/25
Applications
Immunohistochemistry, Flow Cytometry, Immunohistochemistry
Pricing
S100A9, Monoclonal Antibody (Cat# AAA13837)
Full Name
S100A9 (Macrophage Marker) Mouse Monoclonal Antibody
Gene Names
S100A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
Reactivity
Human and Rat. Others not tested.
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(AAA31141 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31141 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
MUC5B, Polyclonal Antibody (Cat# AAA31141)
Full Name
MUC5B Antibody
Gene Names
MUC5B; MG1; MUC5; MUC9; MUC-5B
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
Application Data
(Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Medium power)
CD1a, Monoclonal Antibody (Cat# AAA12011)
Full Name
MOUSE ANTI HUMAN CD1a
Gene Names
CD1A; R4; T6; CD1; FCB6; HTA1
Reactivity
Cynomolgus monkey, Dog
Applications
Immunohistochemistry, Flow Cytometry
Pricing
WB (Western Blot)
(Western Blot Analysis of human MCF-7 cell lysate using ER-beta1 Mouse Monoclonal Antibody (ERb455).)
ER-beta1 (Estrogen Receptor beta-1), Monoclonal Antibody (Cat# AAA13814)
Full Name
ER-beta1 (Estrogen Receptor beta-1) Mouse Monoclonal Antibody
Gene Names
ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
Application Data
(Published customer image: Effect of clustered ephrin-A1-Fc on vascular formation in the rat striatum. Clustered ephrin-A1-Fc was injected into the lesioned side of the lateral ventricle in the unilaterally lesioned rats. Brains taken 6 weeks after injection were sectioned coronally and stained for GFAP (green) and RECA-1 (red) and with DAPI (nuclei; blue). The rectangular insets are shown in Fig. 8B. Scale bar: 100 um.From: Jing X, Miwa H, Sawada T, Nakanishi I, Kondo T, et al. (2012) Ephrin-A1-Mediated Dopaminergic Neurogenesis and Angiogenesis in a Rat Model of Parkinson's Disease. PLoS ONE 7(2): e32019.)
RECA-1, Monoclonal Antibody (Cat# AAA12018)
Full Name
MOUSE ANTI RAT RECA-1
Applications
Immunohistochemistry, Immunofluorescence
Pricing
Application Data
(Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detaction of aromatase in human tissues by Immunohistochemistry on paraffin sectionsImage caption:Morphology and P450 arom immunoreactivity of tumoral region in human testis with seminoma. A-B: Haematoxylin-eosin staining. C-D: Strong P450 arom immunoreactivity in cytoplasm of neoplastic cells (Nc) and unstained lymphocytes (L). Insert: absorption control. Scale bars: A, 20 um; B-C, 12.5 um; D, 5 um.From: Rago V, Romeo F, Aquila S, Montanaro D, And S, Carpino A. Cytochrome P450 aromatase expression in human seminoma. Reprod Biol Endocrinol. 2005 Dec 22;3:72.)
CYTOCHROME P450 AROMATASE, Monoclonal Antibody (Cat# AAA12113)
Full Name
MOUSE ANTI HUMAN CYTOCHROME P450 AROMATASE
Gene Names
CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12081)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
Western Blot
Pricing
Application Data
(Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)
TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12232)
Full Name
RAT ANTI TUBULIN ALPHA:HRP
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
(Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)
TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12009)
Full Name
RAT ANTI TUBULIN ALPHA
Applications
Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western Blot
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12195)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using PP2A-B56δ/PR61δ/PP2A-B56δ/PR61δ/PPP2R5D antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
PP2A-B56delta/PR61delta/PPP2R5D, Polyclonal Antibody (Cat# AAA28319)
Full Name
PP2A-B56delta/PR61delta/PPP2R5D Rabbit pAb
Gene Names
PPP2R5D; B56D
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
NFkappaB p65, Monoclonal Antibody (Cat# AAA29642)
Full Name
NFkappaB p65 Mouse Monoclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification using immunogen.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27698)
Full Name
VPS35 Antibody, Clone 10A8: PerCP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
WB (Western Blot)
(HHLA2 Antibody (N-term) western blot analysis in MDA-MB453,CEM,HL-60,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HHLA2 antibody detected the HHLA2 protein (arrow).)
HHLA2, Polyclonal Antibody (Cat# AAA28722)
Full Name
HHLA2 Antibody (N-term)
Gene Names
HHLA2; B7H7; B7-H7
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(Published customer image: Effect of clustered ephrin-A1-Fc on vascular formation in the rat striatum. Clustered ephrin-A1-Fc was injected into the lesioned side of the lateral ventricle in the unilaterally lesioned rats. Brains taken 6 weeks after injection were sectioned coronally and stained for GFAP (green) and RECA-1 (red) and with DAPI (nuclei; blue). The rectangular insets are shown in Fig. 8B. Scale bar: 100 um.From: Jing X, Miwa H, Sawada T, Nakanishi I, Kondo T, et al. (2012) Ephrin-A1-Mediated Dopaminergic Neurogenesis and Angiogenesis in a Rat Model of Parkinson's Disease. PLoS ONE 7(2): e32019.)
RECA-1, Monoclonal Antibody (Cat# AAA12019)
Full Name
MOUSE ANTI RAT RECA-1
Applications
Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistochemistry)
(AAA31051 at 1/200 staining Human bladder cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
SYK, Polyclonal Antibody (Cat# AAA31051)
Full Name
Phospho-SYK (Tyr348) Antibody
Gene Names
SYK; p72-Syk
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using AAA28677 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
ATG7, Polyclonal Antibody (Cat# AAA28677)
Full Name
ATG7 Antibody (C-term)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human, Mouse
Predicted reactivity: Chicken, Rat
Predicted reactivity: Chicken, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse Beta-glucan Receptor: FITC)
DECTIN-1, Monoclonal Antibody (Cat# AAA12137)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H2B, Polyclonal Antibody (Cat# AAA31352)
Full Name
Acetyl-Histone H2B (Lys20) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Application Data
(Mouse anti Human CD14 antibody, clone Tük4 used to identify bovine monocytes in the skin and subcutaneous tissue of adjuvant injected calves by immunofluorescence.Image caption:Cellular recruitment to skin and subcutaneous tissues. (A) HE stained sections of skin with subcutaneous tissue from the side injected with adjuvant and the contralateral side, at 24 h post-injection. Scale bars: 200 ?m. (B) Enlargement of outlined areas in A, as indicated. Scale bars: 20 ?m. (C) Immunofluorescent labeling of subcutaneous tissue on the injected side with antibody against CD14 (green). Scale bar: 20 ?m.From: Lund H, Boysen P, Åkesson CP, Lewandowska-Sabat AM and Storset AK (2016)Transient Migration of Large Numbers of CD14++ CD16+ Monocytes to the Draining Lymph Node after Onset of Inflammation.Front. Immunol. 7:322.This is from an open access article distributed under the terms of the Creative Commons Attribution License.)
CD14, Monoclonal Antibody (Cat# AAA12266)
Full Name
Mouse Anti Human CD14: Amethyst Orange
Reactivity
Human
Applications
Flow Cytometry
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12095)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:FITC
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12096)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:RPE
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 488)
CD81, Monoclonal Antibody (Cat# AAA11869)
Full Name
HAMSTER ANTI MOUSE CD81:FITC
Gene Names
Cd81; Tapa1; Tapa-1; Tspan28
Applications
Flow Cytometry
Pricing
Application Data
(PE conjugatedMouse anti Human CD169 antibody, clone 7-239 used to block CD169 function on myeloid cells.Image caption:Siglec-1 mediates HIV-1 uptake into a storage compartment and enhances HIV-1 trans-infection specially in IFN?-treated monocytes and DCs. A. Uptake of HIV-1NL4–3 by different myeloid cells exposed to IFN?. Cells were cultured with HIV-1 to measure p24Gag by ELISA. Mean values and SEM from four experiments include cells from 12 donors. B. Fold change in HIV-1NL4–3 uptake of cells treated with bafilomycin A1 compared to untreated cells. Mean values and SEM include cells from three donors. C. Relative uptake of HIV-1NL4–3 by IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 uptake by mock-treated cells (set at 100%). Mean values and SEM from two experiments include cells from six donors. D. Confocal microscopy analysis of different IFN?-treated myeloid cells pulsed with HIV-1Cherry and stained for Siglec-1 (Alexa 488), HLA-DR (Alexa 647) and DAPI. (Top) Representative viral pattern for each kind of myeloid cell analyzed, showing maximum fluorescence intensity of four channels. (Bottom) Percentage of myeloid cells with distinct viral patterns: random distribution, polarized accumulation, and sac-like compartment formation, as illustrated in the left drawing. Mean values of 50 cells from two different donors are shown. E. HIV-1 transmission from IFN?-treated myeloid cells to a luciferase reporter CD4+ cell line. HIV-1 infection was determined by induced luciferase activity in relative light units (RLUs). Mean values and SEM from four experiments include cells from 12 donors. F. Relative HIV-1 transmission from IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 trans-infected by mock-treated cells. Mean values and SEM from two experiments include cells from six donors. Statistical differences were assessed with a paired t test in A and E, and with a one sample t-test in B, C and F.From: Pino M, Erkizia I, Benet S, Erikson E, Fernández-Figueras MT, Guerrero D, Dalmau J, Ouchi D, Rausell A, Ciuffi A, Keppler OT, Telenti A, Kräusslich HG, Martinez-Picado J, Izquierdo-Useros N.HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells.Retrovirology. 2015 May 7;12:37.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
CD169, Monoclonal Antibody (Cat# AAA12265)
Full Name
Mouse Anti Human CD169: RPE
Gene Names
SIGLEC1; SN; CD169; SIGLEC-1
Reactivity
Human
Applications
Flow Cytometry
Purity
>95% by SDS PAGE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
Application Data
(Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 488)
CD81, Monoclonal Antibody (Cat# AAA12033)
Full Name
HAMSTER ANTI MOUSE CD81:RPE
Gene Names
Cd81; Tapa1; Tapa-1; Tspan28
Applications
Flow Cytometry
Pricing
Application Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . Low power)
CD163, Monoclonal Antibody (Cat# AAA11943)
Full Name
MOUSE ANTI HUMAN CD163
Gene Names
CD163; M130; MM130
Reactivity
Guinea Pig, Pig, Rhesus Monkey, Sheep
Applications
Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoassay, Western Blot
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12190)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12189)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:Low Endotoxin
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC AAA12191)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12191)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
Flow Cytometry
Pricing
PAI-1, Monoclonal Antibody (Cat# AAA27728)
Full Name
Anti-PAI-1 Antibody, Mouse Monoclonal
Gene Names
SERPINE1; PAI; PAI1; PAI-1; PLANH1
Applications
ELISA
Purity
Protein A affinity chromatography.
Pricing
FCM (Flow Cytometry)
(Flow Cytometric Analysis of Human Raji Cells. using HLA-Mouse Monoclonal Antibody followed by Goat anti-mouse IgG (Blue); Isotype control (Red).)
HLA-A, Monoclonal Antibody (Cat# AAA13821)
Full Name
HLA-A (MHC I) Mouse Monoclonal Antibody
Gene Names
HLA-B; AS; HLAB; Bw-47; Bw-50; SPDA1; B-4901; B-5001; HLA-Cw; HLA-B15; HLA-B39; HLA-B49; HLA-B50; HLA-B55; HLA-B59; HLA-B61; HEL-S-83; HLA-DRB1; HLA-B*45ZJ; HLA-B-3506; HLA-B-3905; HLA-B-5502; HLA-B-5602
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence
Pricing
Insulin C-terminal Pentapeptide of B-chain, Monoclonal Antibody (Cat# AAA13370)
Full Name
MAb to Insulin C-terminal
Gene Names
INS; ILPR; IRDN; IDDM2; MODY10
Applications
ELISA
Purity
>90% pure (SDS-PAGE). Protein A chromatography
Pricing
ICC (Immunocytochemistry)
(Immunofluorescence staining of U87MG cells using CD68 Mouse Monoclonal Antibody (C68/684) followed by goat anti-Mouse IgG conjugated to (green). Nuclei are stained with Red dot)
CD68, Antibody (Cat# AAA13807)
Full Name
CD68 (Macrophage Marker) Mouse Monoclonal Antibody
Gene Names
CD68; GP110; LAMP4; SCARD1
Reactivity
Human, Monkey, Mouse, and Rat. Others not known.
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27696)
Full Name
VPS35 Antibody, Clone 10A8: FITC
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5Y cells with Tyrosine Hydroxylase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was use as the secondary antibody)
Tyrosine Hydroxylase, Monoclonal Antibody (Cat# AAA30209)
Full Name
Tyrosine Hydroxylase Antibody
Gene Names
TH; TYH; DYT14; DYT5b
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IF (Immunofluorescence)
(AAA31164 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31164 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Pericentrin - Centrosome Marker, Polyclonal Antibody (Cat# AAA31164)
Full Name
Pericentrin - Centrosome Marker Antibody
Gene Names
PCNT; KEN; PCN; MOPD2; PCNT2; PCNTB; PCTN2; SCKL4
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
FCM (Flow Cytometry)
(CB2 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
CB2, Polyclonal Antibody (Cat# AAA28672)
Full Name
CB2 Antibody (C-term)
Gene Names
CNR2; CB2; CX5; CB-2
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data
(Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Medium power)
CD1a, Monoclonal Antibody (Cat# AAA11890)
Full Name
MOUSE ANTI HUMAN CD1a:FITC
Gene Names
CD1A; R4; T6; CD1; FCB6; HTA1
Applications
Flow Cytometry
Pricing
Application Data
(AAA31382 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (AAA31382 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
NF kappaB p105/p50, Polyclonal Antibody (Cat# AAA31382)
Full Name
Phospho-NF kappaB p105/p50 (Ser923) Antibody
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
IHC (Immunohistochemistry)
(AAA31050 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
ZAP-70, Polyclonal Antibody (Cat# AAA31050)
Full Name
Phospho-ZAP-70 (Tyr319) Antibody
Gene Names
ZAP70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing