Search results for "Mouse"

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ACE2, Polyclonal Antibody (Cat# AAA10928)

Full Name

ACE2 Antibody

Gene Names

ACE2; ACEH

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

ACE2 Antibody is affinity chromatography purified via peptide column.

Pricing

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IHC (Immunohistchemistry) (At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

p38 gamma/delta, Polyclonal Antibody (Cat# AAA31407)

Full Name

Phospho-p38 gamma/delta (Tyr185/Tyr182) Antibody

Gene Names

MAPK12; ERK3; ERK6; SAPK3; PRKM12; SAPK-3; P38GAMMA

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Cleaved-Caspase 1, Polyclonal Antibody (Cat# AAA31336)

Full Name

Cleaved-Caspase 1 (Ala317), p10 Antibody

Gene Names

CASP1; ICE; P45; IL1BC

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

GDF15, Monoclonal Antibody (Cat# AAA12357)

Full Name

Rat Monoclonal [clone 6D12.H10.E4] (IgG) to Human GDF15

Gene Names

GDF15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15

Reactivity

Chimpanzee, Macaque

Applications

Immunohistochemistry, Immunohistochemistry, Western Blot

Purity

Protein G Purified chromatography

Pricing

HLA Class 1 Antigen A1,A36, Monoclonal Antibody (Cat# AAA14672)

Full Name

HLA Class 1 Antigen A1,A36 (Biotin)

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified by Ion Exchange chromatography.

Pricing

MLZE, Polyclonal Antibody (Cat# AAA27810)

Full Name

Anti-MLZE Antibody

Gene Names

GSDMC; MLZE

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

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BCMA, Monoclonal Antibody (Cat# AAA14516)

Full Name

BCMA (mouse) monoclonal antibody (Vicky-2)

Gene Names

Tnfrsf17; BCM; BCMA; Tnfrsf13; Tnfrsf13a

Reactivity

Mouse

Applications

Flow Cytometry, Immunoprecipitation, Western Blot

Purity

Protein G-affinity purified.

Pricing

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MT2A, Polyclonal Antibody (Cat# AAA10681)

Full Name

MT2A Polyclonal Antibody

Gene Names

MT2A; MT2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Flow cytometry analysis of LIGHT overexpressing HEK293 cells using LIGHT antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: LIGHT overexpressing HEK293 cells.)

LIGHT, Monoclonal Antibody (Cat# AAA11008)

Full Name

LIGHT Antibody [7B9F12]

Gene Names

TNFSF14; LTg; CD258; HVEML; LIGHT

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Protein A purified

Pricing

ADRM1, Monoclonal Antibody (Cat# AAA24126)

Full Name

ADRM1 (GP110, Proteasomal Ubiquitin Receptor ADRM1, 110kD Cell Membrane Glycoprotein, Adhesion-regulating Molecule 1, Proteasome Regulatory Particle Non-ATPase 13, Rpn13 Homolog, MGC29536) (AP)

Gene Names

ADRM1; ARM1; ARM-1; GP110

Reactivity

Human

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TYR antibody (AAA10704) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

TYR, Polyclonal Antibody (Cat# AAA10704)

Full Name

TYR Polyclonal Antibody

Gene Names

TYR; ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

ACBD3, Monoclonal Antibody (Cat# AAA24118)

Full Name

ACBD3 (Golgi Resident Protein GCP60, Acyl-CoA-binding Domain-containing Protein 3, Golgi Complex-associated Protein 1, GOCAP1, Golgi Phosphoprotein 1, GOLPH1, PBR- and PKA-associated Protein 7, Peripheral Benzodiazepine Receptor-associated Protein PAP7, G

Gene Names

ACBD3; PAP7; GCP60; GOCAP1; GOLPH1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

RLIM, Monoclonal Antibody (Cat# AAA24339)

Full Name

RLIM (RING Finger LIM Domain-binding Protein, R-LIM, E3 Ubiquitin-protein Ligase RLIM, LIM Domain-interacting RING Finger Protein, RING Finger Protein 12, RNF12, Renal Carcinoma Antigen NY-REN-43) (AP)

Gene Names

RLIM; MRX61; RNF12; TOKAS; NY-REN-43

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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CD19, Monoclonal Antibody (Cat# AAA12082)

Full Name

RAT ANTI MOUSE CD19

Gene Names

Cd19; AW495831

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Pricing

UGP2, Monoclonal Antibody (Cat# AAA24401)

Full Name

UGP2 (UTP--glucose-1-phosphate Uridylyltransferase, UDP-glucose Pyrophosphorylase, UDPGP, UGPase, UGP1) (AP)

Gene Names

UGP2; UDPG; UGP1; UDPGP; UGPP1; UGPP2; UDPGP2; pHC379

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (AAA31118 staining 293T by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

GPR133, Polyclonal Antibody (Cat# AAA31118)

Full Name

GPR133 Antibody

Gene Names

ADGRD1; PGR25; GPR133

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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NF kappaB p65, Polyclonal Antibody (Cat# AAA31063)

Full Name

Phospho-NF kappaB p65 (Ser281) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

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CA15-3, Monoclonal Antibody (Cat# AAA13555)

Full Name

CA15-3

Reactivity

Paraffin, frozen

Applications

Immunohistochemistry, Immunohistochemistry

Pricing

HIV-1 gp120, Strain IIIB, Monoclonal Antibody (Cat# AAA14731)

Full Name

Mouse anti-HIV-1 gp120, Strain IIIB (Human Immunodeficiency Virus Type 1) (FITC)

Applications

Dot Blot, Flow Cytometry, Immunohistochemistry, Immunofluorescence

Purity

>95% Purified by gel exclusion chromatography.

Pricing

Prion PrPsc, Monoclonal Antibody (Cat# AAA14607)

Full Name

Prion PrPsc, mAb 1.5D7

Applications

Immunohistochemistry, Immunohistochemistry, Immunoassay, Western Blot

Purity

Protein G

Pricing

SAV1, Monoclonal Antibody (Cat# AAA24353)

Full Name

SAV1 (Protein Salvador Homolog 1, SAV, 45kD WW Domain Protein, WW45, hWW45, WWP4) (AP)

Gene Names

SAV1; SAV; WW45; WWP4

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Kidney Tissue)

Integrin Beta 3 (ITGb3), Polyclonal Antibody (Cat# AAA20173)

Full Name

Polyclonal Antibody to Integrin Beta 3 (ITGb3)

Gene Names

ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Rat testis using LETMD1 Rabbit pAb (AAA10722) at dilution of 1:100 (40x lens).)

LETMD1, Polyclonal Antibody (Cat# AAA10722)

Full Name

LETMD1 Polyclonal Antibody

Gene Names

LETMD1; HCCR1; HCCR-1; HCCR-2; HCRR-2; 1110019O13Rik

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

Cytochrome P450 Aldosterone Synthase, Monoclonal Antibody (Cat# AAA14663)

Full Name

Cytochrome P450 Aldosterone Synthase (CYP11b2)

Gene Names

CYP11B2; CPN2; ALDOS; CYP11B; CYP11BL; CYPXIB2; P450C18; P-450C18; P450aldo

Reactivity

Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Supernatant

Pricing

CDC25C, Monoclonal Antibody (Cat# AAA24164)

Full Name

CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) (AP)

Gene Names

CDC25C; CDC25; PPP1R60

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

RSK3, Monoclonal Antibody (Cat# AAA24347)

Full Name

RSK3, CT (Ribosomal S6 Kinase 3, RSK-3, pp90RSK3, 90kD Ribosomal Protein S6 Kinase 2, p90-RSK 2, p90RSK2, MAP Kinase-activated Protein Kinase 1c, MAPK-activated Protein Kinase 1c, MAPKAP Kinase 1c, MAPKAPK1C, MAPKAPK-1c, Ribosomal Protein S6 Kinase alpha-

Gene Names

RPS6KA2; RSK; HU-2; RSK3; p90RSK2; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (AAA31262 staining H2O2 treated Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31262) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

VGluT1, Polyclonal Antibody (Cat# AAA31262)

Full Name

VGluT1 Antibody

Gene Names

SLC17A7; BNPI; VGLUT1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(80%), Bovine(80%), Horse(80%), Sheep(80%), Rabbit(80%), Dog(80%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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IF (Immunofluorescence) (AAA31240 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31240) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Anillin, Polyclonal Antibody (Cat# AAA31240)

Full Name

Anillin Antibody

Gene Names

ANLN; scra; FSGS8; Scraps

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Dog(100%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

ACTH, Monoclonal Antibody (Cat# AAA12344)

Full Name

Mouse Monoclonal [clone 57] (IgG1) to Human ACTH

Reactivity

Human, Rat

Applications

Immunohistochemistry

Purity

Protein A Purified

Pricing

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IHC (Immunohistochemistry) (Paraffin embedded sections of human urothelium were incubated with anti-human Hsp90 (1:100)for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detectedusing Diaminobenzidine (DAB))

Hsp90, Monoclonal Antibody (Cat# AAA11726)

Full Name

Hsp90 antibody

Gene Names

HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p

Reactivity

Human,

Applications

Western Blot, Immunohistochemistry

Purity

By protein-G affinity chromatography

Pricing

Phospho-SMAD4 (T277), Polyclonal Antibody (Cat# AAA28715)

Full Name

Phospho-SMAD4 (T277) Antibody

Gene Names

SMAD4; JIP; DPC4; MADH4; MYHRS

Reactivity

Human, Mouse
Predicted Species Reactivity: Rat

Applications

Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

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FAK, Polyclonal Antibody (Cat# AAA31066)

Full Name

Phospho-FAK (Tyr576) Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

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FAK, Polyclonal Antibody (Cat# AAA31446)

Full Name

Phospho-FAK (Ser722) Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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AHR, Polyclonal Antibody (Cat# AAA10754)

Full Name

AHR Polyclonal Antibody

Gene Names

AHR; bHLHe76

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity Purification

Pricing

NMNAT-1, Monoclonal Antibody (Cat# AAA11734)

Full Name

NMNAT-1

Gene Names

NMNAT1; LCA9; NMNAT; PNAT1

Reactivity

Human

Applications

Western Blot

Purity

Protein-A affinity chromatography

Pricing

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE)

CD14, Monoclonal Antibody (Cat# AAA12178)

Full Name

MOUSE ANTI HUMAN CD14:APC

Applications

Flow Cytometry

Pricing

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IF (Immunofluorescence) (AAA31122 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31122) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)

IL-6, Polyclonal Antibody (Cat# AAA31122)

Full Name

IL-6 Antibody

Gene Names

IL6; CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Pricing

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IF (Immunofluorescence) (AAA31134 staining HepG2 cells(30min of 4uM Forskolin treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (AAA31134) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)

PERK, Polyclonal Antibody (Cat# AAA31134)

Full Name

Phospho-PERK (Thr982) Antibody

Gene Names

EIF2AK3; PEK; WRS; PERK

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Peptide ELISA

Purity

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns

Pricing

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IF (Immunofluorescence) (Figure 3. IF analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody PC1/3/PCSK1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-PC1/3/PCSK1 Antibody overnight at 4 degree C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight 488 Conjugated Avidin. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

PC1/3, Polyclonal Antibody (Cat# AAA11585)

Full Name

Anti-PC1/3 antibody

Gene Names

PCSK1; PC1; PC3; NEC1; SPC3; BMIQ12

Reactivity

Reacts with: Human, rat; Predicted to work with: Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Immunogen affinity purified.

Pricing

c-Met, Monoclonal Antibody (Cat# AAA12343)

Full Name

Mouse Monoclonal (IgG2a) to Human c-Met

Gene Names

MET; HGFR; AUTS9; RCCP2; c-Met

Reactivity

Human

Applications

Immunohistochemistry

Purity

Purified

Pricing

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CD8 ALPHA, Monoclonal Antibody (Cat# AAA12049)

Full Name

MOUSE ANTI RAT CD8 ALPHA:RPE

Applications

Flow Cytometry

Pricing

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alpha Tubulin, Polyclonal Antibody (Cat# AAA31344)

Full Name

Acetyl-alpha Tubulin (Lys40) Antibody

Gene Names

TUBA1B; K-ALPHA-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

CD45, Monoclonal Antibody (Cat# AAA12111)

Full Name

MOUSE ANTI DOG CD45

Gene Names

PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180

Reactivity

Dog

Applications

Immunohistochemistry, Flow Cytometry, Immunohistochemistry

Pricing

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IHC (Immunohistchemistry) (DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue)

Cold Inducible RNA Binding Protein, Polyclonal Antibody (Cat# AAA20852)

Full Name

Polyclonal Antibody to Cold Inducible RNA Binding Protein (CIRBP)

Gene Names

CIRBP; CIRP

Reactivity

Human, Mouse, Pig

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using ACVR1C antibody at dilution of 1:100 (40x lens).)

ACVR1C, Polyclonal Antibody (Cat# AAA10695)

Full Name

ACVR1C Polyclonal Antibody

Gene Names

ACVR1C; ALK7; ACVRLK7

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (TSPAN1 Antibody (Center) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)