Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
683 results for " D" - showing 600-650
IHC (Immunohistochemistry)
(At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H2B, Polyclonal Antibody (Cat# AAA31352)
Full Name
Acetyl-Histone H2B (Lys20) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Application Data
(Mouse anti Human CD14 antibody, clone Tük4 used to identify bovine monocytes in the skin and subcutaneous tissue of adjuvant injected calves by immunofluorescence.Image caption:Cellular recruitment to skin and subcutaneous tissues. (A) HE stained sections of skin with subcutaneous tissue from the side injected with adjuvant and the contralateral side, at 24 h post-injection. Scale bars: 200 ?m. (B) Enlargement of outlined areas in A, as indicated. Scale bars: 20 ?m. (C) Immunofluorescent labeling of subcutaneous tissue on the injected side with antibody against CD14 (green). Scale bar: 20 ?m.From: Lund H, Boysen P, Åkesson CP, Lewandowska-Sabat AM and Storset AK (2016)Transient Migration of Large Numbers of CD14++ CD16+ Monocytes to the Draining Lymph Node after Onset of Inflammation.Front. Immunol. 7:322.This is from an open access article distributed under the terms of the Creative Commons Attribution License.)
CD14, Monoclonal Antibody (Cat# AAA12266)
Full Name
Mouse Anti Human CD14: Amethyst Orange
Reactivity
Human
Applications
Flow Cytometry
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12096)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:RPE
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12095)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:FITC
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 488)
CD81, Monoclonal Antibody (Cat# AAA12033)
Full Name
HAMSTER ANTI MOUSE CD81:RPE
Gene Names
Cd81; Tapa1; Tapa-1; Tspan28
Applications
Flow Cytometry
Pricing
Application Data
(PE conjugatedMouse anti Human CD169 antibody, clone 7-239 used to block CD169 function on myeloid cells.Image caption:Siglec-1 mediates HIV-1 uptake into a storage compartment and enhances HIV-1 trans-infection specially in IFN?-treated monocytes and DCs. A. Uptake of HIV-1NL4–3 by different myeloid cells exposed to IFN?. Cells were cultured with HIV-1 to measure p24Gag by ELISA. Mean values and SEM from four experiments include cells from 12 donors. B. Fold change in HIV-1NL4–3 uptake of cells treated with bafilomycin A1 compared to untreated cells. Mean values and SEM include cells from three donors. C. Relative uptake of HIV-1NL4–3 by IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 uptake by mock-treated cells (set at 100%). Mean values and SEM from two experiments include cells from six donors. D. Confocal microscopy analysis of different IFN?-treated myeloid cells pulsed with HIV-1Cherry and stained for Siglec-1 (Alexa 488), HLA-DR (Alexa 647) and DAPI. (Top) Representative viral pattern for each kind of myeloid cell analyzed, showing maximum fluorescence intensity of four channels. (Bottom) Percentage of myeloid cells with distinct viral patterns: random distribution, polarized accumulation, and sac-like compartment formation, as illustrated in the left drawing. Mean values of 50 cells from two different donors are shown. E. HIV-1 transmission from IFN?-treated myeloid cells to a luciferase reporter CD4+ cell line. HIV-1 infection was determined by induced luciferase activity in relative light units (RLUs). Mean values and SEM from four experiments include cells from 12 donors. F. Relative HIV-1 transmission from IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 trans-infected by mock-treated cells. Mean values and SEM from two experiments include cells from six donors. Statistical differences were assessed with a paired t test in A and E, and with a one sample t-test in B, C and F.From: Pino M, Erkizia I, Benet S, Erikson E, Fernández-Figueras MT, Guerrero D, Dalmau J, Ouchi D, Rausell A, Ciuffi A, Keppler OT, Telenti A, Kräusslich HG, Martinez-Picado J, Izquierdo-Useros N.HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells.Retrovirology. 2015 May 7;12:37.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
CD169, Monoclonal Antibody (Cat# AAA12265)
Full Name
Mouse Anti Human CD169: RPE
Gene Names
SIGLEC1; SN; CD169; SIGLEC-1
Reactivity
Human
Applications
Flow Cytometry
Purity
>95% by SDS PAGE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
Application Data
(Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 488)
CD81, Monoclonal Antibody (Cat# AAA11869)
Full Name
HAMSTER ANTI MOUSE CD81:FITC
Gene Names
Cd81; Tapa1; Tapa-1; Tspan28
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12189)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:Low Endotoxin
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
Application Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . Low power)
CD163, Monoclonal Antibody (Cat# AAA11943)
Full Name
MOUSE ANTI HUMAN CD163
Gene Names
CD163; M130; MM130
Reactivity
Guinea Pig, Pig, Rhesus Monkey, Sheep
Applications
Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoassay, Western Blot
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12190)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC AAA12191)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12191)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
Flow Cytometry
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis using the Antibody at 1:50 dilution)
MGEA5, Monoclonal Antibody (Cat# AAA30518)
Full Name
MGEA5 Antibody
Gene Names
MGEA5; OGA; MEA5; NCOAT
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
Purity
Affinity-chromatography
Pricing
ICC (Immunocytochemistry)
(Immunofluorescence staining of U87MG cells using CD68 Mouse Monoclonal Antibody (C68/684) followed by goat anti-Mouse IgG conjugated to (green). Nuclei are stained with Red dot)
CD68, Antibody (Cat# AAA13807)
Full Name
CD68 (Macrophage Marker) Mouse Monoclonal Antibody
Gene Names
CD68; GP110; LAMP4; SCARD1
Reactivity
Human, Monkey, Mouse, and Rat. Others not known.
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry
Pricing
Application Data
(Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Medium power)
CD1a, Monoclonal Antibody (Cat# AAA11890)
Full Name
MOUSE ANTI HUMAN CD1a:FITC
Gene Names
CD1A; R4; T6; CD1; FCB6; HTA1
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA12084)
Full Name
MOUSE ANTI HUMAN CD105:RPE
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
Application Data
(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody , red an A and Mouse anti Rat CD8 MCA48), green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA11981)
Full Name
MOUSE ANTI RAT CD8 ALPHA
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12188)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:Biotin
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA11933)
Full Name
MOUSE ANTI HUMAN CD105
Gene Names
ENG; END; HHT1; ORW1
Reactivity
Cynomolgus monkey, Horse, Monkey, Primate, Rhesus Monkey
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot
Pricing
Double-stranded RNA (dsRNA), Monoclonal ELISA Kit (Cat# AAA14576)
Full Name
Double-stranded RNA (dsRNA) ELISA kit (J2 based)
Reactivity
General
Applications
ELISA
Pricing
Application Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
IFN GAMMA, Monoclonal Antibody (Cat# AAA12094)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:Biotin
Applications
Flow Cytometry
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12196)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
Application Data
(Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12199)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
Flow Cytometry
Pricing
Vitamin D (25 OH Vitamin D), Monoclonal Antibody (Cat# AAA13443)
Full Name
Monoclonal Antibody to Human Vitamin D (25 OH Vitamin D)
Applications
Chemiluminescent Immunoassay, Lateral Flow, Radioimmunoassay
Purity
Protein A Chromatography
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of LPHN2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
LPHN2, Polyclonal Antibody (Cat# AAA27788)
Full Name
Anti-LPHN2 Antibody
Gene Names
ADGRL2; CL2; LEC1; CIRL2; LPHH1; LPHN2
Reactivity
Human, Mouse, Rat, Bovine
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohistochemistry)
(IHC image of AAA27004 diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
GBA, Polyclonal Antibody (Cat# AAA27004)
Full Name
GBA Antibody
Gene Names
GBA; GCB; GBA1; GLUC
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
>95%, Protein G purified
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11930)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11864)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
Immunofluorescence
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Ki67 Mouse Monoclonal Antibody (MKI67/2466). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Ki-67, Monoclonal Antibody (Cat# AAA23909)
Full Name
Ki-67 (Proliferating Cell Marker)
Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human. Others not known.
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA12085)
Full Name
MOUSE ANTI HUMAN CD105
Gene Names
ENG; END; HHT1; ORW1
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot
Pricing
TCR gamma delta, Monoclonal Antibody (Cat# AAA17922)
Full Name
TCR gamma delta antibody
Reactivity
Recognizes the gamma/delta T-cell receptor (TCR).
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Pricing
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
CRYGD, Recombinant Protein (Cat# AAA11809)
Full Name
CRYGD, 1-174aa, Human, His tag, E Coli
Gene Names
CRYGD; CCP; PCC; CACA; CCA3; CRYG4; CTRCT4; cry-g-D
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
HSV1 gD, Recombinant Protein (Cat# AAA14363)
Full Name
HSV1 gD protein
Applications
Western Blot
Purity
> 95% by SDS PAGE
Pricing
WB (Western Blot)
(Western blot analysis of CD298 expression in HEK293T (A), Hela (B), A2780 (C), mouse heart (D), mouse liver (E), rat heart (F), rat liver (G) whole cell lysates.(Predicted band size: 31 kD; Observed band size: 40 kD))
CD298, Polyclonal Antibody (Cat# AAA27791)
Full Name
Anti-CD298 Antibody
Gene Names
ATP1B3; CD298; ATPB-3
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: CYPD knockout A549 cell lysate; Predicted MW: 41kd Observed MW: 43kd Primary Ab: 3ug/ml Rabbit Anti-Human CYPD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Cyclophilin D (CYPD), Polyclonal Antibody (Cat# AAA20302)
Full Name
Polyclonal Antibody to Cyclophilin D (CYPD)
Gene Names
PPID; CYPD; CYP-40
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Affinity Chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells cells using AAA15913 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Alpha-enolase, Polyclonal Antibody (Cat# AAA15913)
Full Name
Rabbit anti- human Alpha-enolase polyclonal Antibody
Gene Names
ENO1; NNE; PPH; MPB1; ENO1L1; HEL-S-17
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12211)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA12029)
Full Name
MOUSE ANTI HUMAN CD105:RPE
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA12083)
Full Name
MOUSE ANTI HUMAN CD105:FITC
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)
CD105, Monoclonal Antibody (Cat# AAA11865)
Full Name
MOUSE ANTI HUMAN CD105:FITC
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
Application Data
(Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 488)
CD81, Monoclonal Antibody (Cat# AAA11942)
Full Name
HAMSTER ANTI MOUSE CD81
Gene Names
Cd81; Tapa1; Tapa-1; Tspan28
Reactivity
Rat
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin embedded rat thymus sections using AAA14692 at a 1:250 dilution and detected by Alexa-488, which appears fluorescent green. 8-oxo-dG is present in the cytoplasm and nucleus of most but not all cells. Sections were counter-stained with 7-Aminoactinomycin D (7AAD), which labels nuclear DNA. Images were captured at 40X magnification.)
8-Hydroxy-2'-deoxyguanosine, Monoclonal Antibody (Cat# AAA14692)
Full Name
Mouse anti-8-Hydroxy-2'-deoxyguanosine (8-oxo-dG)
Applications
Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Purity
Purified from ascites.
Pricing
WB (Western Blot)
(Western Blot analysis for galactocerebroside in PC12 lysate. PC12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with AAA14685 at 1:500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein Galactocerebroside (~75kD).)
Galactocerebroside, Monoclonal Antibody (Cat# AAA14685)
Full Name
Mouse anti-Bovine Galactocerebroside
Applications
Immunohistochemistry, Immunocytochemistry
Purity
Purified by Protein A affinity chromatography.
Pricing
IF (Immunofluorescence)
(AAA31275 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31275 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
LIF, Polyclonal Antibody (Cat# AAA31275)
Full Name
LIF Antibody
Gene Names
LIF; CDF; DIA; HILDA; MLPLI
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Bovine(88%), Sheep(88%), Dog(88%)
Predicted Reactivity: Pig(88%), Bovine(88%), Sheep(88%), Dog(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various tissue sample, using Phospho-PLCG1 (Tyr771) Antibody.)
PLCG1, Polyclonal Antibody (Cat# AAA31021)
Full Name
Phospho-PLCG1 (Tyr771) Antibody
Gene Names
PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Beta-3 Adrenergic Receptor expression in HEK293T (A), Hela (B), HepG2 (C), A2780 (D), mouse kidney (E), rat liver (F) whole cell lysates.)
Beta-3 Adrenergic Receptor, Polyclonal Antibody (Cat# AAA27792)
Full Name
Anti-Beta-3 Adrenergic Receptor Antibody
Gene Names
ADRB3; BETA3AR
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
Application Data
(TNFR2/TNFRSF1B-mediated inhibition of cytotoxicity was Neutralized by Human TNFR2 Antibody. Recombinant Human TNFR2/TNFRSF1B inhibits Recombinant Human TNFa induced cytotoxicity in the L-929 mouse fibroblast cell line. Inhibition of Recombinant Human TNFa (0.25 ng/mL) activity elicited by Recombinant Human TNFR2/TNFRSF1B (1 ug/mL) is neutralized by increasing concentrations of Human TNFR2/TNFRSF1B Monoclonal Antibody. The IC50 is typically 1-3 ug/mL in the presence of the metabolic inhibitor actinomycin D (1 ug/mL).)
TNFR2/CD120b/TNFRSF1B, Monoclonal Antibody (Cat# AAA27733)
Full Name
TNFR2/CD120b/TNFRSF1B Neutralizing Antibody
Gene Names
TNFRSF1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
Reactivity
Human
Applications
Blocking, Neutralization
Purity
>95% SDS-PAGE
Pricing
Application Data
(The extracellular domain is required for Sln1 kinase activation by wall perturbations. A, representative field showing localization of SLN1-GFP (pSS1881) or sln1ΔECD-GFP (pSS1904). BF, bright field. B, Western analysis showing reduced levels of sln1ΔECD compared with full-length SLN1. SLN1, JF2007 (sln1Δ) carrying full-length SLN1-myc (pCLM994); sln1ΔECD, JF2007 carrying sln1ΔECD-myc (pCLM1774). C, comparison of Hog1 phosphorylation kinetics in SLN1 (JF2008 (sln1Δ sho1Δ) carrying full-length (pCLM994)) and sln1ΔECD (JF2008 (sln1Δ sho1Δ) carrying sln1ΔECD (pCLM1774)) strains following addition of 0.4 M NaCl. D, Northern (RNA) hybridization analysis of RNA prepared from wild type cultures from the sln1Δ strain, JF2007, or sln1Δ ccw12Δ strain, JF2368, transformed with the SLN1 expression plasmids, pCLM994 (SLN1) or pCLM1774 (sln1ΔECD), and treated with 1 unit/ml zymolyase (+) where indicated. NCA3 expression was normalized to expression of the SLN1-SKN7-independent CDC33 gene. The extent of induction by zymolyase is shown relative to the untreated SLN1 or sln1ΔECD strains.)
Zymolyase 100T Concentrate 10mg/ml, 0.1M Sorbitol, Enzyme (Cat# AAA14837)
Full Name
Zymolyase 100T Concentrate 10mg/ml, 0.1M Sorbitol (Lyticase, Yeast Lytic Enzyme)
Pricing
IF (Immunofluorescence)
(Figure 7. IF analysis of CD68 using anti- CD68 antibody CD68 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti- CD68 Antibody overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
CD68, Polyclonal Antibody (Cat# AAA11512)
Full Name
Anti-CD68 antibody
Gene Names
Cd68; Lamp4; gp110; Scard1
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11850)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
Immunohistochemistry, Western Blot
Pricing
Hepatitis D Virus Antigen (HDVAg), ELISA Kit (Cat# AAA10551)
Full Name
Qualitative Human Hepatitis D Virus Antigen (HDVAg) ELISA Kit
Reactivity
Human
Pricing