Search results for " Cell"

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WB (Western Blot) (Western Blot analysis of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody (M03), clone 1B11.Lane 1: HMGB1 transfected lysate (25 KDa).Lane 2: Non-transfected lysate.)

HMGB1, Monoclonal Antibody (Cat# AAA26381)

Full Name

HMGB1 (High-Mobility Group Box 1, DKFZp686A04236, HMG1, HMG3, SBP-1) (FITC)

Gene Names

HMGB1; HMG1; HMG3; HMG-1; SBP-1

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

Clara Cell Protein 16 (CC16), ELISA Kit (Cat# AAA20486)

Full Name

Human Clara Cell Protein 16 (CC16) ELISA Kit

Reactivity

Human

Pricing

Chymotrypsin-like elastase family member 2A, Recombinant Protein (Cat# AAA18604)

Full Name

Recombinant Human Chymotrypsin-like elastase family member 2A

Gene Names

CELA2A; PE-1; ELA2A

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

Tetanus toxin (tetX), Recombinant Protein (Cat# AAA18711)

Full Name

Recombinant Clostridium tetani Tetanus toxin (tetX), partial

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

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IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to ABL2 on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 1.5 ug/ml])

ABL2, Monoclonal Antibody (Cat# AAA26347)

Full Name

ABL2 (v-abl Abelson murine Leukemia Viral OncoGene Homolog 2 (arg, Abelson-Related Gene), ABLL, ARG, FLJ22224, FLJ31718, FLJ41441) (FITC)

Gene Names

ABL2; ARG; ABLL

Applications

IF, IHC, WB

Purity

Purified

Pricing

Cytolysin (vvhA), Recombinant Protein (Cat# AAA18605)

Full Name

Recombinant Vibrio vulnificus Cytolysin (vvhA)

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

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IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 1.2 ug/ml])

TAF11, Monoclonal Antibody (Cat# AAA26084)

Full Name

TAF11 (TAF11 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 28kD, MGC:15243, PRO2134, TAF2I, TAFII28) (APC)

Gene Names

TAF11; TAF2I; PRO2134; TAFII28; MGC:15243

Applications

IF, IHC, WB

Purity

Purified

Pricing

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Application Data (Detection limit for recombinant GST tagged TAF11 is approximately 0.1ng/ml as a capture antibody.)

TAF11, Monoclonal Antibody (Cat# AAA26351)

Full Name

TAF11 (TAF11 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 28kD, MGC:15243, PRO2134, TAF2I, TAFII28) (FITC)

Gene Names

TAF11; TAF2I; PRO2134; TAFII28; MGC:15243

Applications

IF, IHC, WB

Purity

Purified

Pricing

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Application Data (Detection limit for recombinant GST tagged TAF11 is approximately 0.1ng/ml as a capture antibody.)

TAF11, Monoclonal Antibody (Cat# AAA26616)

Full Name

TAF11 (TAF11 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 28kD, MGC:15243, PRO2134, TAF2I, TAFII28) (PE)

Gene Names

TAF11; TAF2I; PRO2134; TAFII28; MGC:15243

Applications

IF, IHC, WB

Purity

Purified

Pricing

Annexin A2 (ANXA2), ELISA Kit (Cat# AAA20335)

Full Name

Mouse Annexin A2 (ANXA2) ELISA Kit

Reactivity

Mouse

Pricing

Cytosolic 5'-nucleotidase 1A (NT5C1A), Recombinant Protein (Cat# AAA18721)

Full Name

Recombinant Human Cytosolic 5'-nucleotidase 1A (NT5C1A)

Gene Names

NT5C1A; CN1; CNI; CN-I; CN1A; CN-IA

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

Pneumolysin (ply), Recombinant Protein (Cat# AAA18677)

Full Name

Recombinant Streptococcus pneumoniae serotype 2 Pneumolysin (ply)

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

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WB (Western Blot) (Western blot analysis of SNX1 over-expressed 293 cell line, cotransfected with SNX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SNX1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

SNX1, Monoclonal Antibody (Cat# AAA25258)

Full Name

SNX1 (Sorting Nexin-1, MGC8664) (FITC)

Gene Names

SNX1; VPS5; HsT17379

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

NADPH--cytochrome P450 reductase (POR), Recombinant Protein (Cat# AAA18522)

Full Name

Recombinant Human NADPH--cytochrome P450 reductase (POR), partial

Gene Names

POR; CPR; CYPOR; P450R

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

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WB (Western Blot) (Western blot analysis of NDN over-expressed 293 cell line, cotransfected with NDN Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NDN monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

NDN, Monoclonal Antibody (Cat# AAA25761)

Full Name

NDN (Necdin) (PE)

Gene Names

NDN; PWCR; HsT16328

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (TXNDC4 monoclonal antibody Western Blot analysis of TXNDC4 expression in K-562.)

Thioredoxin Domain-containing Protein 4, Monoclonal Antibody (Cat# AAA24990)

Full Name

Thioredoxin Domain-containing Protein 4 (TXNDC4, Endoplasmic Reticulum Resident Protein 44, ER Protein 44, ERp44, KIAA0573, PDIA10, UNQ532/PRO1075) (Biotin)

Gene Names

ERP44; PDIA10; TXNDC4

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (NEK11 monoclonal antibody. Western Blot analysis of NEK11 expression in A-431.)

NEK11, Monoclonal Antibody (Cat# AAA25766)

Full Name

NEK11 (Serine/Threonine-protein Kinase Nek11, Never in Mitosis A-related Kinase 11, NimA-related Protein Kinase 11, FLJ23495) (PE)

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged MPG is ~0.03ng/ml as a capture antibody.)

MPG, Monoclonal Antibody (Cat# AAA24278)

Full Name

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (AP)

Gene Names

MPG; AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DUSP6 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

DUSP6, Monoclonal Antibody (Cat# AAA24785)

Full Name

DUSP6 (Dual Specificity Protein Phosphatase 6, Dual Specificity Protein Phosphatase PYST1, Mitogen-activated Protein Kinase Phosphatase 3, MAP Kinase Phosphatase 3, MKP3, MKP-3, PYST1) (Biotin)

Gene Names

DUSP6; HH19; MKP3; PYST1

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (ALS2CR8 monoclonal antibody, Western Blot analysis of ALS2CR8 expression in Hela NE.)

ALS2CR8, Monoclonal Antibody (Cat# AAA25320)

Full Name

ALS2CR8 (CARF, Amyotrophic Lateral Sclerosis 2 Chromosomal Region Candidate Gene 8 Protein, Calcium-response Factor, Testis Development Protein NYD-SP24, DKFZp667N246, FLJ12675, FLJ21579) (HRP)

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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DB (Dot Blot) (Dot blot analysis using Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507). Tissue: Cell lysates. Species: Human. Primary Antibody: Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507) at 1:500, 1:5000. Beta Amyloid HEPES-NaCl aggregation, showing 1:500 (L) and 1:5000 (R) time lapse dot blot.)

Amyloid Fibrils (OC), Polyclonal Antibody (Cat# AAA17798)

Full Name

Amyloid Fibrils (OC) Antibody: ATTO 390

Reactivity

Human. Potentially mouse and rat based on species homology.

Applications

IP, ICC, IHC, EIA, WB, DB

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)

Hsp70/Hsc70, Monoclonal Antibody (Cat# AAA17780)

Full Name

Hsp70/Hsc70 Antibody: Biotin

Gene Names

HSPA2; HSP70

Reactivity

Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast

Applications

WB, IP, IHC

Pricing

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WB (Western Blot) (Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.)

Hsp90, Monoclonal Antibody (Cat# AAA17787)

Full Name

Hsp90 Antibody: RPE

Gene Names

HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B

Reactivity

Human (beta-specific), Rabbit (Beta Specific), Chicken (Alpha/Beta), Rat, Canine

Applications

WB, IP, EIA, IHC, IF

Pricing

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WB (Western Blot) (BUB1B monoclonal antibody Western Blot analysis of BUB1B expression in Hela NE.)

BUBR1, Monoclonal Antibody (Cat# AAA24739)

Full Name

BUBR1 (Mitotic Checkpoint Serine/Threonine-protein Kinase BUB1 beta, MAD3/BUB1-related Protein Kinase, hBUBR1, Mitotic Checkpoint Kinase MAD3L, Protein SSK1, BUB1B, MAD3L, SSK1) (Biotin)

Gene Names

BUB1B; MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon cancer using AAA18859 at dilution of 1:100)

Cellular tumor antigen p53, Polyclonal Antibody (Cat# AAA18859)

Full Name

Rabbit anti-human Cellular tumor antigen p53 polyclonal Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

EIA, WB, IHC

Purity

Caprylic Acid Ammonium Sulfate Precipitation Purified

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (AAA19172).Musashi 1/Msi1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Musashi 1/Msi1 Antibody (AAA19172) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Musashi 1/Msi1, Polyclonal Antibody (Cat# AAA19172)

Full Name

Anti-Musashi 1/Msi1 Picoband Antibody

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins

Applications

IHC, WB

Purity

Immunogen affinity purified

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HEPA1-6 cells using anti-PAR4/Pawr antibody (AAA19278).Overlay histogram showing HEPA1-6 cells stained with AAA19278 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAR4/Pawr Antibody (AAA19278, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PAR4/Pawr, Polyclonal Antibody (Cat# AAA19278)

Full Name

Anti-PAR4/Pawr Antibody

Gene Names

Pawr; PAR4; Par-4; 2310001G03Rik

Reactivity

Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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CDC27, Polyclonal Antibody (Cat# AAA19285)

Full Name

Anti-CDC27 Antibody

Gene Names

CDC27; APC3; HNUC; NUC2; ANAPC3; CDC27Hs; D0S1430E; D17S978E

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of TAGLN/Transgelin using anti-TAGLN/Transgelin antibody (AAA19286).TAGLN/Transgelin was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TAGLN/Transgelin Antibody (AAA19286) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

TAGLN/Transgelin, Polyclonal Antibody (Cat# AAA19286)

Full Name

Anti-TAGLN/Transgelin Antibody

Gene Names

TAGLN; SM22; SMCC; TAGLN1; WS3-10

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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Application Data (Published customer image: Mouse anti Human CD49d antibody, clone HP2/1 used for binding efficiency determinationImage caption:Binding efficiencies (BE) of different a4beta7 molecules composed of distinct a4 mutants to monoclonal antibodies against a4, beta7 or the a4beta7 heterodimer. Binding efficiency is determined by the ratio between the mean fluorescence of antibody binding to each a4 molecule and of the binding in a mock-transfected cell culture (see Materials and Methods for details). Dark gray bars represent binding to the human (wild type) a4 clone, whereas light gray bars are those of binding to the different a4 mutants (as shown in the x-axis). a4 mutants which included substitutions at codon 201 are boxed. A, binding of anti-a4 2b4 antibody. B, binding of anti-a4 HP2/1 antibody. C, BE of different anti-a4 and beta7 antibodies to the human a4 and the quintuple a4 mutant (5 aa mut). Bars represent the range of standard errors deduced from triplicate experiments. p-values of Student's t tests are shown above each comparison. NS, non-significant (> 0.05).From:Darc M, Hait SH, Soares EA, Cicala C, Seuanez HN, et al. (2011) Polymorphisms in the a4 Integrin of Neotropical Primates: Insights for Binding of Natural Ligands and HIV-1 gp120 to the Human a4beta7. PLoS ONE 6(9): e24461.)

Application Data (Published customer image: Mouse anti Human CD49d antibody, clone HP2/1 used for western blotting and immunoprecipitationImage caption:Tetraspanins CD81, CD82 and CD151 are associated with a4beta1 throughout erythroid maturation and with beta3 in proerythroblasts and basophilic erythroblasts. A. CD81, CD82 and CD151 precipitates from Mn2+-activated proerythroblasts (ProEB, day 5), basophilic (BasoEB, day 8) and polychromatic (PolyEB, day 12) erythroblasts were successively probed with anti-a4, anti-beta1 and anti-beta3 antibodies; tetraspanin controls from each time point are also illustrated. All tetraspanins co-precipitated a4 and beta1 from erythroblasts B. Tetraspanin precipitates from day 6 proerythroblasts (ProEB) solubilised in the presence of EDTA or different cations, and from Mn2+-activated basophilic erythroblasts (BasoEB, day 8) were probed with a mix of antibodies to a5, beta1, beta2 and beta3 integrins while the control samples were probed with the relevant tetraspanin antibodies. For clarity, integrin controls are illustrated for the EDTA blot but were present on all blots. beta1 and beta3 integrins were precipitated well only in the presence of Mn2+. C. CD81 and CD82 precipitates from day 5 proerythroblasts were successively probed with different anti-integrin subunit antibodies and demonstrate co-precipitation of beta1 and beta3 but not a5 or beta2 integrins. D. CD81 (454720) and CD82 (53H5) precipitates from day 6 proerythroblasts (ProEB) and HEL cells (HEL) solubilised in the presence of EDTA, Ca2++Mg2+ or Mn2+ probed with anti-CD82 and anti-CD81 antibodies. Each tetraspanin co-precipitates the other most strongly in the presence of Mn2+ from proerythroblasts while any cation permits co-precipitation in HEL cells. Integrins were analysed on 7.5% gels, tetraspanins on 12% gels; non-reducing conditions. Unless stated, the following clones were used: CD53, MEM-53; CD63, MEM-259; CD81, 454720; CD82, TS82b; CD151, IIG5a; a4, HP2/1; a5, IIA1; aL, TS1/22; aIIb, PAB-1. All day 5 and 6 cultures comprised 90 -95% proerythroblasts; day 8 culture comprised 5% proerythroblasts, 81% basophilic erythroblasts and 14% polychromatic erythroblasts; day 12 culture comprised 41% polychromatic erythroblasts, 15% orthochromatic erythroblasts and 41% reticulocytes. In the day 5 and 6 cultures 15 -34% of cells were GPA+ and 28 -35% of cells were aIIb+. Day 8 and day 12 cultures had 77% and 97% GPA+ cells, respectively, and 9% and 0% aIIb+ cells, respectively.From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, et al. (2013) Tetraspanins CD81 and CD82 Facilitate a4beta1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1. PLoS ONE 8(5): e62654.)