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Reactivity
Gene Name
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Application
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2304 results for "Secondary Antibody" - showing 500-550
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of GAPDH Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
GAPDH, Polyclonal Antibody (Cat# AAA28776)
Full Name
GAPDH Antibody (N-term)
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(Figure 8. IHC analysis of FOXP1 using anti-FOXP1 antibody (AAA19227).FOXP1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-FOXP1 Antibody (AAA19227) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
FOXP1, Polyclonal Antibody (Cat# AAA19227)
Full Name
Anti-FOXP1 Antibody
Gene Names
FOXP1; QRF1; 12CC4; hFKH1B; HSPC215
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Caspase 3 Antibody for IHC in human colon tissue)
Caspase 3, Polyclonal Antibody (Cat# AAA31093)
Full Name
Caspase 3 Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of SiHa cells using anti-POR antibody (AAA11661).Overlay histogram showing SiHa cells stained with AAA11661 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POR Antibody (AAA11661,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
POR, Polyclonal Antibody (Cat# AAA11661)
Full Name
Anti-POR Antibody
Gene Names
POR; CPR; CYPOR; P450R
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human mammary cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Jak2, Polyclonal Antibody (Cat# AAA31311)
Full Name
Phospho-Jak2 (Tyr1008) Antibody
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Strength of Signal
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin (VIM) Mouse Monoclonal Antibody (VM452)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Vimentin, Monoclonal Antibody (Cat# AAA13810)
Full Name
Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody
Gene Names
VIM; HEL113; CTRCT30
Reactivity
Human, Cow, Dog, Cat, Pig, Goat, Chicken.
Does not react with Mouse and Rat
Does not react with Mouse and Rat
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H2B, Polyclonal Antibody (Cat# AAA31351)
Full Name
Phospho-Histone H2B (Tyr43) Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
ERK1/2, Polyclonal Antibody (Cat# AAA31300)
Full Name
Phospho-ERK1/2 (Thr177/Thr160) Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
NFAT2, Polyclonal Antibody (Cat# AAA31428)
Full Name
Phospho-NFAT2 (Ser172) Antibody
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Nucleophosmin, Polyclonal Antibody (Cat# AAA31308)
Full Name
Phospho-Nucleophosmin (Ser125) Antibody
Gene Names
NPM1; B23; NPM
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)
EMR1, Monoclonal Antibody (Cat# AAA26723)
Full Name
EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (AP)
Reactivity
Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded Human gastric using Lamin A/C antibody at dilution of 1:100 (40x lens).)
LMNA, Polyclonal Antibody (Cat# AAA10641)
Full Name
LMNA Polyclonal Antibody
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of N2A cells with Choline Acetyltransferase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Choline Acetyltransferase, Monoclonal Antibody (Cat# AAA30341)
Full Name
Choline Acetyltransferase Antibody
Gene Names
CHAT; CMS1A; CMS1A2; CHOACTASE
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-Zyxin (Tyr316) in lysates of Jurkat paclitaxel 1uM 24h, using Phospho-Zyxin (Tyr316) Antibody.)
Zyxin, Polyclonal Antibody (Cat# AAA31158)
Full Name
Phospho-Zyxin (Tyr316) Antibody
Gene Names
ZYX; ESP-2; HED-2
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HL-60 cells with GEF H1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
GEF H1, Monoclonal Antibody (Cat# AAA30493)
Full Name
GEF H1 Antibody
Gene Names
ARHGEF2; GEF; P40; GEFH1; LFP40; GEF-H1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Niban, Polyclonal Antibody (Cat# AAA31335)
Full Name
Niban Antibody
Gene Names
FAM129A; GIG39; NIBAN; C1orf24
Reactivity
Human, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (92%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (92%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
HP1 alpha, Polyclonal Antibody (Cat# AAA31332)
Full Name
Phospho-HP1 alpha (Ser92) Antibody
Gene Names
CBX5; HP1; HP1A
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
RSK1/2/3/4, Polyclonal Antibody (Cat# AAA31315)
Full Name
Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody
Gene Names
RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
CREB, Polyclonal Antibody (Cat# AAA31303)
Full Name
Phospho-CREB (Ser129) Antibody
Gene Names
CREB1; CREB
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
TDP43, Polyclonal Antibody (Cat# AAA31323)
Full Name
Phospho-TDP43 (Ser409/Ser410) Antibody
Gene Names
TARDBP; ALS10; TDP-43
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
S100A9, Polyclonal Antibody (Cat# AAA31466)
Full Name
Phospho-S100A9 (Thr113) Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western BlotPositive WB detected in Recombinant proteinAll lanes: dnaK antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionpredicted band size: 70 kDaobserved band size: 70 kDa)
dnaK, Polyclonal Antibody (Cat# AAA26988)
Full Name
dnaK Antibody
Reactivity
Escherichia coli
Applications
Western Blot
Purity
> 95%, Protein G purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of Jurkat cells using CASP3 antibody at dilution of 1:100. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours (left). Blue: DAPI for nuclear staining.)
CASP3, Polyclonal Antibody (Cat# AAA28248)
Full Name
CASP3 Polyclonal Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using MCL1 antibody at dilution of 1:100 (40x lens).)
MCL1, Polyclonal Antibody (Cat# AAA10630)
Full Name
MCL1 Polyclonal Antibody
Gene Names
MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5Y cells with PDK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
PDK2, Monoclonal Antibody (Cat# AAA30453)
Full Name
PDK2 Antibody
Gene Names
PDK2; PDHK2; PDKII
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with PKC beta 2 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
PKC beta 2, Monoclonal Antibody (Cat# AAA30098)
Full Name
PKC beta 2 Antibody
Gene Names
PRKCB; PKCB; PRKCB1; PRKCB2; PKC-beta
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of 293T cells with XBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
XBP1, Monoclonal Antibody (Cat# AAA30306)
Full Name
XBP1 Antibody
Gene Names
XBP1; XBP2; TREB5; XBP-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5Y cells with MYLK antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Myosin Light Chain Kinase, Monoclonal Antibody (Cat# AAA30080)
Full Name
Myosin Light Chain Kinase Antibody
Gene Names
MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of NIH/3T3 cells with eIF4EBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
eIF4EBP1, Monoclonal Antibody (Cat# AAA30251)
Full Name
eIF4EBP1 Antibody
Gene Names
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells with SCF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
SCF, Monoclonal Antibody (Cat# AAA30142)
Full Name
SCF Antibody
Gene Names
KITLG; SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
USF1, Polyclonal Antibody (Cat# AAA31437)
Full Name
Phospho-USF1 (Thr153) Antibody
Gene Names
USF1; UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (100%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
((3.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
KCNN4/KCa3.1, Antibody (Cat# AAA13678)
Full Name
Goat Anti-KCNN4/KCa3.1 Antibody
Gene Names
KCNN4; IK; IK1; SK4; DHS2; KCA4; hSK4; IKCA1; hKCa4; KCa3.1; hIKCa1
Reactivity
Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog
Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Peptide ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
(Anti-HA Tag mouse monoclonal antibody at 1:1000 dilutionLane A: HA-GST (Recombinant protein)(30ng)Lane B: GST-HA (Recombinant protein)(10ng)Lane C: HA-ARG1-myc transfected 293 cell lysate (2ug)Lane D: HA-mFABP4-myc transfected 293 cell lysate (2ug)Lane E: myc-mFABP4-HA transfected 293 cell lysate (0.5ug)Lane F: myc-ARG1-HA transfected 293 cell lysate (2ug)SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)
HA, Monoclonal Antibody (Cat# AAA27712)
Full Name
Anti-HA tag Antibody, Mouse Monoclonal
Reactivity
HA tag
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HL-60 cells using anti-Sumo 1/SUMO1 antibody (AAA19224).Overlay histogram showing HL-60 cells stained with AAA19224 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sumo 1/SUMO1 Antibody (AAA19224, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Sumo 1/SUMO1, Polyclonal Antibody (Cat# AAA19224)
Full Name
Anti-Sumo 1/SUMO1 Antibody
Gene Names
SUMO1; DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H2A, Polyclonal Antibody (Cat# AAA31347)
Full Name
Acetyl-Histone H2A (Lys5) Antibody
Gene Names
HIST1H2AB; H2A/m; H2AFM
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using PRKCA antibody.)
PRKCA , Monoclonal Antibody (Cat# AAA28235)
Full Name
PRKCA Monoclonal Antibody
Gene Names
PRKCA; AAG6; PKCA; PRKACA; PKC-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Overlay Peak curve showing 293F cells transfected with GST stained with AAA28065 (red line) at 1:189. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1?g/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1?g/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
GST, Monoclonal Antibody (Cat# AAA28065)
Full Name
GST Monoclonal Antibody
Reactivity
All
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein A purified
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of C9JLR9 Antibody (C-term) (AAA28790) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
C9JLR9, Polyclonal Antibody (Cat# AAA28790)
Full Name
C9JLR9 Antibody (C-term)
Reactivity
Human
Predicted Reactivity: Mouse
Predicted Reactivity: Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Transferrin Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Transferrin (CD71), Monoclonal Antibody (Cat# AAA30263)
Full Name
Transferrin Receptor (CD71) Antibody
Gene Names
TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation analysis extracts of 293 cell line, using TriMethyl-Histone H3-K4 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
H3K4me3, Antibody (Cat# AAA10662)
Full Name
Histone H3K4me3 Polyclonal Antibody
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse lung using ABCB7 antibody at dilution of 1:100 (40x lens).)
ABCB7, Polyclonal Antibody (Cat# AAA28279)
Full Name
ABCB7 Polyclonal Antibody
Gene Names
ABCB7; ABC7; ASAT; Atm1p; EST140535
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Smad5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
SMAD5, Monoclonal Antibody (Cat# AAA30038)
Full Name
SMAD5 Antibody
Gene Names
SMAD5; DWFC; JV5-1; MADH5
Reactivity
Human, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(HSPA5 Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
HSPA5, Polyclonal Antibody (Cat# AAA28738)
Full Name
HSPA5 Antibody
Gene Names
HSPA5; BIP; MIF2; GRP78; HEL-S-89n
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(Immunochemical staining MSH6 in Ferret spleen with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
MSH6, Monoclonal Antibody (Cat# AAA27721)
Full Name
Recombinant Anti-MSH6 Antibody, Rabbit Monoclonal
Gene Names
MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5
Reactivity
Human, Cynomolgus, Mouse, Rat, Ferret
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with USP14 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Usp14, Monoclonal Antibody (Cat# AAA30408)
Full Name
Usp14 Antibody
Gene Names
USP14; TGT
Reactivity
Human, Zebrafish
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various celllines, using GSK3 alpha/beta Antibody.)
GSK3 alpha/beta, Polyclonal Antibody (Cat# AAA31094)
Full Name
GSK3 alpha/beta Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of PC-3M cells with hUPF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
hUPF1, Monoclonal Antibody (Cat# AAA30506)
Full Name
hUPF1 Antibody
Gene Names
UPF1; HUPF1; NORF1; RENT1; smg-2; pNORF1
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
OTUB1, Polyclonal Antibody (Cat# AAA31284)
Full Name
Phospho-OTUB1 (Ser16) Antibody
Gene Names
OTUB1; OTB1; OTU1; HSPC263
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of A431 cells using anti-BIK antibody (AAA11666).Overlay histogram showing A431 cells stained with AAA11666 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BIK Antibody (AAA11666,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Bik, Polyclonal Antibody (Cat# AAA11666)
Full Name
Anti-Bik Antibody
Gene Names
BIK; BP4; NBK; BIP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using CD10 Mouse Monoclonal Antibody (MME/1870)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
CD10, Monoclonal Antibody (Cat# AAA23901)
Full Name
CD10 (Membrane Metalloendopeptidase)
Gene Names
MME; NEP; SFE; CD10; CALLA; CMT2T; SCA43
Reactivity
Human. Others not tested.
Applications
Immunohistochemistry
Pricing